Amino acid dipepetide frequency for Leptolyngbya sp. NIES-2104

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.089AlaAla: 8.089 ± 0.089	0.805AlaCys: 0.805 ± 0.024	4.197AlaAsp: 4.197 ± 0.053	5.698AlaGlu: 5.698 ± 0.07	3.377AlaPhe: 3.377 ± 0.044	5.727AlaGly: 5.727 ± 0.078	1.367AlaHis: 1.367 ± 0.031	8.306AlaIle: 8.306 ± 0.066	3.792AlaLys: 3.792 ± 0.051	9.3AlaLeu: 9.3 ± 0.079	1.895AlaMet: 1.895 ± 0.029	3.231AlaAsn: 3.231 ± 0.053	3.617AlaPro: 3.617 ± 0.047	4.863AlaGln: 4.863 ± 0.059	4.348AlaArg: 4.348 ± 0.052	4.907AlaSer: 4.907 ± 0.063	4.967AlaThr: 4.967 ± 0.06	5.704AlaVal: 5.704 ± 0.057	1.098AlaTrp: 1.098 ± 0.021	2.209AlaTyr: 2.209 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	0.686CysAla: 0.686 ± 0.02	0.154CysCys: 0.154 ± 0.01	0.627CysAsp: 0.627 ± 0.017	0.545CysGlu: 0.545 ± 0.017	0.403CysPhe: 0.403 ± 0.015	0.766CysGly: 0.766 ± 0.021	0.243CysHis: 0.243 ± 0.012	0.46CysIle: 0.46 ± 0.017	0.289CysLys: 0.289 ± 0.013	1.017CysLeu: 1.017 ± 0.027	0.141CysMet: 0.141 ± 0.008	0.293CysAsn: 0.293 ± 0.013	0.518CysPro: 0.518 ± 0.017	0.476CysGln: 0.476 ± 0.018	0.576CysArg: 0.576 ± 0.018	0.613CysSer: 0.613 ± 0.017	0.456CysThr: 0.456 ± 0.015	0.61CysVal: 0.61 ± 0.021	0.151CysTrp: 0.151 ± 0.009	0.324CysTyr: 0.324 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.215AspAla: 4.215 ± 0.047	0.536AspCys: 0.536 ± 0.018	2.463AspAsp: 2.463 ± 0.05	3.091AspGlu: 3.091 ± 0.048	2.248AspPhe: 2.248 ± 0.037	3.334AspGly: 3.334 ± 0.054	0.905AspHis: 0.905 ± 0.023	2.64AspIle: 2.64 ± 0.047	1.45AspLys: 1.45 ± 0.03	6.067AspLeu: 6.067 ± 0.073	0.794AspMet: 0.794 ± 0.023	1.421AspAsn: 1.421 ± 0.034	2.97AspPro: 2.97 ± 0.042	2.744AspGln: 2.744 ± 0.037	5.881AspArg: 5.881 ± 0.062	2.987AspSer: 2.987 ± 0.042	2.283AspThr: 2.283 ± 0.048	3.165AspVal: 3.165 ± 0.046	0.973AspTrp: 0.973 ± 0.027	1.659AspTyr: 1.659 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	5.989GluAla: 5.989 ± 0.075	0.446GluCys: 0.446 ± 0.018	2.622GluAsp: 2.622 ± 0.043	3.576GluGlu: 3.576 ± 0.05	2.634GluPhe: 2.634 ± 0.042	3.205GluGly: 3.205 ± 0.045	1.055GluHis: 1.055 ± 0.026	4.39GluIle: 4.39 ± 0.049	2.9GluLys: 2.9 ± 0.047	7.02GluLeu: 7.02 ± 0.075	1.438GluMet: 1.438 ± 0.031	2.261GluAsn: 2.261 ± 0.038	2.668GluPro: 2.668 ± 0.04	3.815GluGln: 3.815 ± 0.053	3.724GluArg: 3.724 ± 0.052	3.691GluSer: 3.691 ± 0.047	3.913GluThr: 3.913 ± 0.047	4.336GluVal: 4.336 ± 0.053	0.801GluTrp: 0.801 ± 0.02	1.537GluTyr: 1.537 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	3.448PheAla: 3.448 ± 0.048	0.521PheCys: 0.521 ± 0.017	2.493PheAsp: 2.493 ± 0.04	2.397PheGlu: 2.397 ± 0.031	1.663PhePhe: 1.663 ± 0.038	3.152PheGly: 3.152 ± 0.045	0.778PheHis: 0.778 ± 0.02	2.014PheIle: 2.014 ± 0.037	1.373PheLys: 1.373 ± 0.027	4.058PheLeu: 4.058 ± 0.054	0.669PheMet: 0.669 ± 0.02	1.586PheAsn: 1.586 ± 0.035	1.862PhePro: 1.862 ± 0.033	1.871PheGln: 1.871 ± 0.031	2.158PheArg: 2.158 ± 0.036	3.081PheSer: 3.081 ± 0.043	2.314PheThr: 2.314 ± 0.045	2.742PheVal: 2.742 ± 0.042	0.739PheTrp: 0.739 ± 0.021	1.309PheTyr: 1.309 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	5.366GlyAla: 5.366 ± 0.071	0.724GlyCys: 0.724 ± 0.022	3.394GlyAsp: 3.394 ± 0.066	3.865GlyGlu: 3.865 ± 0.045	3.159GlyPhe: 3.159 ± 0.047	4.792GlyGly: 4.792 ± 0.091	1.197GlyHis: 1.197 ± 0.032	4.744GlyIle: 4.744 ± 0.057	3.294GlyLys: 3.294 ± 0.047	6.989GlyLeu: 6.989 ± 0.083	1.577GlyMet: 1.577 ± 0.028	2.747GlyAsn: 2.747 ± 0.079	0.924GlyPro: 0.924 ± 0.024	3.174GlyGln: 3.174 ± 0.042	3.751GlyArg: 3.751 ± 0.05	4.336GlySer: 4.336 ± 0.057	4.299GlyThr: 4.299 ± 0.065	4.666GlyVal: 4.666 ± 0.06	1.172GlyTrp: 1.172 ± 0.028	2.178GlyTyr: 2.178 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	1.259HisAla: 1.259 ± 0.032	0.294HisCys: 0.294 ± 0.013	0.89HisAsp: 0.89 ± 0.023	0.93HisGlu: 0.93 ± 0.023	0.839HisPhe: 0.839 ± 0.021	1.075HisGly: 1.075 ± 0.026	0.602HisHis: 0.602 ± 0.023	0.908HisIle: 0.908 ± 0.025	0.522HisLys: 0.522 ± 0.017	2.268HisLeu: 2.268 ± 0.042	0.268HisMet: 0.268 ± 0.014	0.566HisAsn: 0.566 ± 0.019	1.328HisPro: 1.328 ± 0.03	1.166HisGln: 1.166 ± 0.029	1.281HisArg: 1.281 ± 0.028	1.22HisSer: 1.22 ± 0.029	0.895HisThr: 0.895 ± 0.022	0.881HisVal: 0.881 ± 0.02	0.387HisTrp: 0.387 ± 0.014	0.691HisTyr: 0.691 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	8.093IleAla: 8.093 ± 0.074	0.628IleCys: 0.628 ± 0.019	4.293IleAsp: 4.293 ± 0.042	5.025IleGlu: 5.025 ± 0.048	2.08IlePhe: 2.08 ± 0.036	4.667IleGly: 4.667 ± 0.067	1.188IleHis: 1.188 ± 0.023	2.458IleIle: 2.458 ± 0.039	1.995IleLys: 1.995 ± 0.039	6.021IleLeu: 6.021 ± 0.064	0.783IleMet: 0.783 ± 0.023	2.158IleAsn: 2.158 ± 0.039	3.342IlePro: 3.342 ± 0.041	3.275IleGln: 3.275 ± 0.04	3.63IleArg: 3.63 ± 0.047	3.921IleSer: 3.921 ± 0.056	3.284IleThr: 3.284 ± 0.048	5.121IleVal: 5.121 ± 0.066	0.803IleTrp: 0.803 ± 0.023	1.593IleTyr: 1.593 ± 0.028	0.001IleXaa: 0.001 ± 0.001
Lys	3.583LysAla: 3.583 ± 0.047	0.214LysCys: 0.214 ± 0.01	1.794LysAsp: 1.794 ± 0.035	2.118LysGlu: 2.118 ± 0.035	1.474LysPhe: 1.474 ± 0.032	2.243LysGly: 2.243 ± 0.033	0.729LysHis: 0.729 ± 0.019	2.446LysIle: 2.446 ± 0.039	1.545LysLys: 1.545 ± 0.042	4.563LysLeu: 4.563 ± 0.054	0.796LysMet: 0.796 ± 0.024	1.336LysAsn: 1.336 ± 0.031	2.351LysPro: 2.351 ± 0.039	2.368LysGln: 2.368 ± 0.041	2.454LysArg: 2.454 ± 0.034	2.609LysSer: 2.609 ± 0.041	2.562LysThr: 2.562 ± 0.038	2.684LysVal: 2.684 ± 0.038	0.417LysTrp: 0.417 ± 0.017	0.945LysTyr: 0.945 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	9.008LeuAla: 9.008 ± 0.076	1.014LeuCys: 1.014 ± 0.025	5.717LeuAsp: 5.717 ± 0.055	7.039LeuGlu: 7.039 ± 0.067	3.894LeuPhe: 3.894 ± 0.055	7.053LeuGly: 7.053 ± 0.066	1.889LeuHis: 1.889 ± 0.037	6.812LeuIle: 6.812 ± 0.066	5.254LeuLys: 5.254 ± 0.052	10.855LeuLeu: 10.855 ± 0.101	2.339LeuMet: 2.339 ± 0.038	4.521LeuAsn: 4.521 ± 0.053	5.69LeuPro: 5.69 ± 0.053	5.405LeuGln: 5.405 ± 0.059	6.17LeuArg: 6.17 ± 0.067	7.992LeuSer: 7.992 ± 0.068	6.245LeuThr: 6.245 ± 0.055	6.828LeuVal: 6.828 ± 0.074	1.455LeuTrp: 1.455 ± 0.035	2.629LeuTyr: 2.629 ± 0.035	0.0LeuXaa: 0.0 ± 0.0
Met	1.68MetAla: 1.68 ± 0.03	0.132MetCys: 0.132 ± 0.009	0.769MetAsp: 0.769 ± 0.021	0.932MetGlu: 0.932 ± 0.022	0.629MetPhe: 0.629 ± 0.019	1.322MetGly: 1.322 ± 0.031	0.277MetHis: 0.277 ± 0.011	1.269MetIle: 1.269 ± 0.027	0.93MetLys: 0.93 ± 0.023	2.014MetLeu: 2.014 ± 0.03	0.488MetMet: 0.488 ± 0.015	0.936MetAsn: 0.936 ± 0.024	1.082MetPro: 1.082 ± 0.023	1.095MetGln: 1.095 ± 0.024	1.17MetArg: 1.17 ± 0.027	1.57MetSer: 1.57 ± 0.026	1.473MetThr: 1.473 ± 0.024	1.287MetVal: 1.287 ± 0.033	0.173MetTrp: 0.173 ± 0.008	0.357MetTyr: 0.357 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.345AsnAla: 3.345 ± 0.056	0.39AsnCys: 0.39 ± 0.015	1.729AsnAsp: 1.729 ± 0.047	1.707AsnGlu: 1.707 ± 0.034	1.535AsnPhe: 1.535 ± 0.03	2.738AsnGly: 2.738 ± 0.055	0.775AsnHis: 0.775 ± 0.023	1.804AsnIle: 1.804 ± 0.032	0.909AsnLys: 0.909 ± 0.024	4.679AsnLeu: 4.679 ± 0.065	0.521AsnMet: 0.521 ± 0.015	1.223AsnAsn: 1.223 ± 0.032	2.66AsnPro: 2.66 ± 0.04	2.475AsnGln: 2.475 ± 0.037	2.531AsnArg: 2.531 ± 0.037	2.462AsnSer: 2.462 ± 0.039	1.876AsnThr: 1.876 ± 0.035	2.16AsnVal: 2.16 ± 0.037	0.648AsnTrp: 0.648 ± 0.019	1.181AsnTyr: 1.181 ± 0.026	0.001AsnXaa: 0.001 ± 0.001
Pro	3.705ProAla: 3.705 ± 0.047	0.339ProCys: 0.339 ± 0.015	3.222ProAsp: 3.222 ± 0.043	3.979ProGlu: 3.979 ± 0.055	1.835ProPhe: 1.835 ± 0.031	3.072ProGly: 3.072 ± 0.051	0.858ProHis: 0.858 ± 0.025	3.287ProIle: 3.287 ± 0.042	2.058ProLys: 2.058 ± 0.037	4.414ProLeu: 4.414 ± 0.047	0.915ProMet: 0.915 ± 0.022	2.29ProAsn: 2.29 ± 0.035	2.283ProPro: 2.283 ± 0.048	2.301ProGln: 2.301 ± 0.043	2.033ProArg: 2.033 ± 0.037	3.037ProSer: 3.037 ± 0.045	3.11ProThr: 3.11 ± 0.049	3.38ProVal: 3.38 ± 0.048	0.61ProTrp: 0.61 ± 0.017	1.246ProTyr: 1.246 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	5.256GlnAla: 5.256 ± 0.062	0.382GlnCys: 0.382 ± 0.015	2.174GlnAsp: 2.174 ± 0.036	3.047GlnGlu: 3.047 ± 0.047	2.267GlnPhe: 2.267 ± 0.034	3.29GlnGly: 3.29 ± 0.046	1.004GlnHis: 1.004 ± 0.026	3.761GlnIle: 3.761 ± 0.046	2.161GlnLys: 2.161 ± 0.04	5.933GlnLeu: 5.933 ± 0.071	1.08GlnMet: 1.08 ± 0.026	1.959GlnAsn: 1.959 ± 0.032	2.675GlnPro: 2.675 ± 0.046	3.642GlnGln: 3.642 ± 0.057	3.326GlnArg: 3.326 ± 0.045	3.638GlnSer: 3.638 ± 0.051	3.336GlnThr: 3.336 ± 0.044	3.82GlnVal: 3.82 ± 0.051	0.736GlnTrp: 0.736 ± 0.022	1.292GlnTyr: 1.292 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	4.22ArgAla: 4.22 ± 0.05	0.543ArgCys: 0.543 ± 0.017	3.3ArgAsp: 3.3 ± 0.04	3.607ArgGlu: 3.607 ± 0.046	2.771ArgPhe: 2.771 ± 0.041	3.331ArgGly: 3.331 ± 0.049	1.103ArgHis: 1.103 ± 0.025	3.861ArgIle: 3.861 ± 0.04	2.263ArgLys: 2.263 ± 0.037	6.836ArgLeu: 6.836 ± 0.067	1.305ArgMet: 1.305 ± 0.029	2.269ArgAsn: 2.269 ± 0.038	2.412ArgPro: 2.412 ± 0.038	3.48ArgGln: 3.48 ± 0.048	3.573ArgArg: 3.573 ± 0.047	5.521ArgSer: 5.521 ± 0.059	3.067ArgThr: 3.067 ± 0.044	4.006ArgVal: 4.006 ± 0.042	0.942ArgTrp: 0.942 ± 0.024	2.021ArgTyr: 2.021 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	5.396SerAla: 5.396 ± 0.063	0.576SerCys: 0.576 ± 0.016	3.878SerAsp: 3.878 ± 0.047	4.094SerGlu: 4.094 ± 0.054	2.625SerPhe: 2.625 ± 0.04	5.003SerGly: 5.003 ± 0.072	1.267SerHis: 1.267 ± 0.026	4.527SerIle: 4.527 ± 0.05	2.272SerLys: 2.272 ± 0.038	7.118SerLeu: 7.118 ± 0.065	1.379SerMet: 1.379 ± 0.026	2.509SerAsn: 2.509 ± 0.044	3.465SerPro: 3.465 ± 0.05	3.458SerGln: 3.458 ± 0.043	3.785SerArg: 3.785 ± 0.048	4.424SerSer: 4.424 ± 0.06	3.874SerThr: 3.874 ± 0.05	4.266SerVal: 4.266 ± 0.05	0.892SerTrp: 0.892 ± 0.022	1.715SerTyr: 1.715 ± 0.032	0.001SerXaa: 0.001 ± 0.001
Thr	5.308ThrAla: 5.308 ± 0.062	0.485ThrCys: 0.485 ± 0.016	2.71ThrAsp: 2.71 ± 0.042	3.383ThrGlu: 3.383 ± 0.045	2.166ThrPhe: 2.166 ± 0.042	4.322ThrGly: 4.322 ± 0.057	1.036ThrHis: 1.036 ± 0.023	4.233ThrIle: 4.233 ± 0.052	1.883ThrLys: 1.883 ± 0.037	6.362ThrLeu: 6.362 ± 0.06	0.879ThrMet: 0.879 ± 0.026	1.968ThrAsn: 1.968 ± 0.037	3.472ThrPro: 3.472 ± 0.067	2.984ThrGln: 2.984 ± 0.051	2.861ThrArg: 2.861 ± 0.042	3.317ThrSer: 3.317 ± 0.04	3.327ThrThr: 3.327 ± 0.063	4.397ThrVal: 4.397 ± 0.052	0.735ThrTrp: 0.735 ± 0.022	1.482ThrTyr: 1.482 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	5.729ValAla: 5.729 ± 0.052	0.656ValCys: 0.656 ± 0.021	3.487ValAsp: 3.487 ± 0.05	4.503ValGlu: 4.503 ± 0.053	2.721ValPhe: 2.721 ± 0.047	4.474ValGly: 4.474 ± 0.059	1.093ValHis: 1.093 ± 0.024	4.143ValIle: 4.143 ± 0.055	2.82ValLys: 2.82 ± 0.041	7.321ValLeu: 7.321 ± 0.069	1.523ValMet: 1.523 ± 0.031	2.477ValAsn: 2.477 ± 0.046	3.142ValPro: 3.142 ± 0.041	3.392ValGln: 3.392 ± 0.04	4.031ValArg: 4.031 ± 0.043	4.51ValSer: 4.51 ± 0.048	3.935ValThr: 3.935 ± 0.053	4.681ValVal: 4.681 ± 0.071	0.915ValTrp: 0.915 ± 0.024	1.728ValTyr: 1.728 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	0.979TrpAla: 0.979 ± 0.023	0.162TrpCys: 0.162 ± 0.01	0.593TrpAsp: 0.593 ± 0.02	0.812TrpGlu: 0.812 ± 0.021	0.678TrpPhe: 0.678 ± 0.02	0.865TrpGly: 0.865 ± 0.023	0.318TrpHis: 0.318 ± 0.011	1.062TrpIle: 1.062 ± 0.025	0.695TrpLys: 0.695 ± 0.022	1.787TrpLeu: 1.787 ± 0.037	0.42TrpMet: 0.42 ± 0.013	0.699TrpAsn: 0.699 ± 0.021	0.065TrpPro: 0.065 ± 0.006	1.088TrpGln: 1.088 ± 0.025	0.95TrpArg: 0.95 ± 0.027	0.94TrpSer: 0.94 ± 0.025	0.74TrpThr: 0.74 ± 0.019	0.882TrpVal: 0.882 ± 0.024	0.259TrpTrp: 0.259 ± 0.013	0.429TrpTyr: 0.429 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.14TyrAla: 2.14 ± 0.039	0.345TyrCys: 0.345 ± 0.013	1.472TyrAsp: 1.472 ± 0.033	1.669TyrGlu: 1.669 ± 0.037	1.231TyrPhe: 1.231 ± 0.028	1.915TyrGly: 1.915 ± 0.034	0.587TyrHis: 0.587 ± 0.019	1.233TyrIle: 1.233 ± 0.025	0.824TyrLys: 0.824 ± 0.025	3.189TyrLeu: 3.189 ± 0.04	0.376TyrMet: 0.376 ± 0.016	0.9TyrAsn: 0.9 ± 0.025	1.447TyrPro: 1.447 ± 0.028	1.708TyrGln: 1.708 ± 0.03	2.2TyrArg: 2.2 ± 0.04	1.749TyrSer: 1.749 ± 0.033	1.356TyrThr: 1.356 ± 0.031	1.665TyrVal: 1.665 ± 0.032	0.517TyrTrp: 0.517 ± 0.016	0.881TyrTyr: 0.881 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6684 proteins (1864961 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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