Thiobacillus denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Thiobacillaceae; Thiobacillus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3078 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A106BP43|A0A106BP43_THIDE Protease OS=Thiobacillus denitrificans OX=36861 GN=ABW22_08315 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.1HH3 pKa = 6.93LIPEE7 pKa = 4.57TPPDD11 pKa = 3.76YY12 pKa = 11.24DD13 pKa = 3.14HH14 pKa = 6.79TRR16 pKa = 11.84IIEE19 pKa = 4.29RR20 pKa = 11.84PDD22 pKa = 2.75GCYY25 pKa = 9.95WIDD28 pKa = 3.45ADD30 pKa = 4.03TGEE33 pKa = 4.62EE34 pKa = 4.14YY35 pKa = 11.27GPFPSMLEE43 pKa = 3.74AVQDD47 pKa = 3.56MEE49 pKa = 4.73YY50 pKa = 10.7NAEE53 pKa = 4.02SDD55 pKa = 4.01FEE57 pKa = 4.85PGEE60 pKa = 4.24TVEE63 pKa = 4.23EE64 pKa = 4.26AEE66 pKa = 4.22EE67 pKa = 4.25EE68 pKa = 4.01IGISDD73 pKa = 5.04WIDD76 pKa = 3.59PDD78 pKa = 3.36TGEE81 pKa = 4.7PGEE84 pKa = 4.17EE85 pKa = 3.35AHH87 pKa = 7.07RR88 pKa = 11.84PVEE91 pKa = 4.03

Molecular weight: 10.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A125BC38|A0A125BC38_THIDE Cytidylate kinase OS=Thiobacillus denitrificans OX=36861 GN=cmk PE=3 SV=1
MM1 pKa = 7.53FEE3 pKa = 4.63HH4 pKa = 7.02FSDD7 pKa = 4.47ALILADD13 pKa = 3.42AEE15 pKa = 4.36ARR17 pKa = 11.84VSYY20 pKa = 10.19LNPAAEE26 pKa = 4.33RR27 pKa = 11.84LLGLPLNNTRR37 pKa = 11.84GHH39 pKa = 6.78ALDD42 pKa = 5.0KK43 pKa = 11.24VLTLQDD49 pKa = 3.21GLTRR53 pKa = 11.84QSIQIGDD60 pKa = 4.25FPTQHH65 pKa = 6.46SPFSGAFHH73 pKa = 6.74LLVRR77 pKa = 11.84NGGSAIPMQCSVALTRR93 pKa = 11.84TGSGATGGYY102 pKa = 9.19MIVLRR107 pKa = 11.84NASDD111 pKa = 3.7LQQHH115 pKa = 6.12IDD117 pKa = 3.47KK118 pKa = 10.92LVTQSMHH125 pKa = 7.11DD126 pKa = 3.3EE127 pKa = 4.04HH128 pKa = 8.44SRR130 pKa = 11.84LLRR133 pKa = 11.84RR134 pKa = 11.84AEE136 pKa = 3.84LVKK139 pKa = 10.73RR140 pKa = 11.84LWRR143 pKa = 11.84LLQEE147 pKa = 4.52ADD149 pKa = 3.41GGEE152 pKa = 4.23PQAFMYY158 pKa = 10.96LDD160 pKa = 4.2LDD162 pKa = 3.81NFKK165 pKa = 10.93SVNDD169 pKa = 4.11MAGHH173 pKa = 6.89AAGDD177 pKa = 3.53LAIRR181 pKa = 11.84QIAARR186 pKa = 11.84LKK188 pKa = 9.15DD189 pKa = 3.59QVRR192 pKa = 11.84GRR194 pKa = 11.84DD195 pKa = 3.2TLARR199 pKa = 11.84LGGMNSACCWSAVRR213 pKa = 11.84RR214 pKa = 11.84SARR217 pKa = 11.84ASVLSNCIGRR227 pKa = 11.84WNPKK231 pKa = 9.43SCAGTAKK238 pKa = 9.35STGWVSASASPFSKK252 pKa = 9.56RR253 pKa = 11.84TSTARR258 pKa = 11.84TAFWPRR264 pKa = 11.84RR265 pKa = 11.84MRR267 pKa = 11.84RR268 pKa = 11.84AIRR271 pKa = 11.84PSVMAVMGPTYY282 pKa = 10.13RR283 pKa = 11.84KK284 pKa = 9.0SHH286 pKa = 4.52STEE289 pKa = 3.62RR290 pKa = 3.59

Molecular weight: 31.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3075

3

3078

919040

27

3500

298.6

32.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.323 ± 0.059

0.887 ± 0.016

5.552 ± 0.034

5.549 ± 0.041

3.603 ± 0.029

7.921 ± 0.043

2.395 ± 0.028

4.816 ± 0.03

3.527 ± 0.042

11.109 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.021

2.831 ± 0.027

5.023 ± 0.032

3.903 ± 0.032

6.688 ± 0.046

5.064 ± 0.033

5.106 ± 0.034

7.381 ± 0.037

1.386 ± 0.02

2.422 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski