Xingshan nematode virus 4

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Alphanemrhavirus; Xingshan alphanemrhavirus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KN93|A0A1L3KN93_9RHAB GDP polyribonucleotidyltransferase OS=Xingshan nematode virus 4 OX=1923763 PE=4 SV=1
MM1 pKa = 7.34TEE3 pKa = 3.57GRR5 pKa = 11.84LFFEE9 pKa = 4.79PEE11 pKa = 4.12DD12 pKa = 3.65VKK14 pKa = 10.73STNWIPQQANYY25 pKa = 10.69LKK27 pKa = 9.92TDD29 pKa = 3.29LRR31 pKa = 11.84KK32 pKa = 10.26DD33 pKa = 3.55RR34 pKa = 11.84EE35 pKa = 4.42LTGMDD40 pKa = 4.31DD41 pKa = 4.11LEE43 pKa = 4.65SSNFLDD49 pKa = 4.71DD50 pKa = 5.64DD51 pKa = 4.78VYY53 pKa = 11.51SPPPRR58 pKa = 11.84IPFGEE63 pKa = 4.42LSQDD67 pKa = 3.3TLKK70 pKa = 10.11TVQPVEE76 pKa = 4.23NPSGNTSPGFTTYY89 pKa = 10.91KK90 pKa = 8.91FTPIGDD96 pKa = 3.56NPEE99 pKa = 3.86FQRR102 pKa = 11.84RR103 pKa = 11.84LVSEE107 pKa = 4.11TLKK110 pKa = 11.03DD111 pKa = 3.74LEE113 pKa = 4.41KK114 pKa = 10.95LHH116 pKa = 6.86LFTIIHH122 pKa = 5.64EE123 pKa = 4.19QRR125 pKa = 11.84EE126 pKa = 4.26NQSLVFQLKK135 pKa = 9.78RR136 pKa = 11.84KK137 pKa = 7.17TRR139 pKa = 11.84FPSTHH144 pKa = 6.42SGISEE149 pKa = 3.94INPEE153 pKa = 4.1EE154 pKa = 4.22RR155 pKa = 11.84RR156 pKa = 11.84SSVPTDD162 pKa = 3.18QLRR165 pKa = 11.84EE166 pKa = 3.89IVQEE170 pKa = 3.73KK171 pKa = 9.95SRR173 pKa = 11.84IYY175 pKa = 10.59SVQTKK180 pKa = 9.47ARR182 pKa = 11.84KK183 pKa = 9.31IVDD186 pKa = 4.16DD187 pKa = 4.65FPCPVKK193 pKa = 10.47MIGASGSPFKK203 pKa = 11.1INWKK207 pKa = 9.33EE208 pKa = 3.65INVQLEE214 pKa = 4.19QEE216 pKa = 4.27LMAFTEE222 pKa = 4.46EE223 pKa = 4.09EE224 pKa = 4.24LEE226 pKa = 4.14EE227 pKa = 4.35LGKK230 pKa = 10.73LSHH233 pKa = 7.34DD234 pKa = 4.44RR235 pKa = 11.84ILGKK239 pKa = 10.63LLIKK243 pKa = 10.56NHH245 pKa = 6.28LSSPVTKK252 pKa = 10.15LISYY256 pKa = 9.94IYY258 pKa = 10.51FEE260 pKa = 4.3

Molecular weight: 30.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KNG7|A0A1L3KNG7_9RHAB Uncharacterized protein OS=Xingshan nematode virus 4 OX=1923763 PE=4 SV=1
MM1 pKa = 7.71NYY3 pKa = 10.49LPILRR8 pKa = 11.84RR9 pKa = 11.84LNKK12 pKa = 10.16NGNRR16 pKa = 11.84NEE18 pKa = 3.92NRR20 pKa = 11.84NDD22 pKa = 3.24NTKK25 pKa = 10.56NPSNLGEE32 pKa = 4.1NFKK35 pKa = 10.55PKK37 pKa = 9.95KK38 pKa = 10.22DD39 pKa = 3.54QTFLTDD45 pKa = 4.02FPKK48 pKa = 11.27GEE50 pKa = 4.13FKK52 pKa = 11.0NFNSIQTSSRR62 pKa = 11.84SPQLYY67 pKa = 8.94FDD69 pKa = 5.21KK70 pKa = 10.74IEE72 pKa = 4.18KK73 pKa = 10.1PIVEE77 pKa = 4.7LSCGPKK83 pKa = 10.02FSKK86 pKa = 10.5EE87 pKa = 4.17YY88 pKa = 9.94IEE90 pKa = 4.41KK91 pKa = 10.11VHH93 pKa = 5.48YY94 pKa = 9.02TLNLEE99 pKa = 4.24VNAEE103 pKa = 4.08VTILTTKK110 pKa = 10.64RR111 pKa = 11.84LTIQEE116 pKa = 4.25IAGHH120 pKa = 5.48LLEE123 pKa = 4.59HH124 pKa = 7.05RR125 pKa = 11.84EE126 pKa = 4.0RR127 pKa = 11.84FVGDD131 pKa = 3.1LRR133 pKa = 11.84IYY135 pKa = 9.62PIYY138 pKa = 9.94HH139 pKa = 5.54ITMLLCLHH147 pKa = 6.16NCKK150 pKa = 10.06HH151 pKa = 6.0QDD153 pKa = 2.83ITRR156 pKa = 11.84MEE158 pKa = 4.6FKK160 pKa = 10.38HH161 pKa = 6.1QYY163 pKa = 7.67SNHH166 pKa = 5.84MEE168 pKa = 4.02TVLQINTKK176 pKa = 9.45FSKK179 pKa = 11.06DD180 pKa = 3.32NVISKK185 pKa = 10.56NGVAYY190 pKa = 9.88RR191 pKa = 11.84VEE193 pKa = 4.1WTNLGIDD200 pKa = 4.09YY201 pKa = 10.19KK202 pKa = 9.63LHH204 pKa = 5.0WMFRR208 pKa = 11.84TFLKK212 pKa = 9.49KK213 pKa = 7.81TTVKK217 pKa = 10.58GISPTYY223 pKa = 9.45QQFPLLPYY231 pKa = 10.11FLEE234 pKa = 4.61ASALSVCLKK243 pKa = 7.8EE244 pKa = 4.17TNLIITQQ251 pKa = 3.63

Molecular weight: 29.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3625

251

2169

725.0

83.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.614 ± 0.582

2.207 ± 0.332

4.579 ± 0.342

6.345 ± 0.544

5.241 ± 0.228

5.103 ± 0.259

3.09 ± 0.23

7.531 ± 0.502

6.179 ± 0.786

11.752 ± 0.686

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.848 ± 0.318

5.103 ± 0.681

4.69 ± 0.312

3.476 ± 0.328

4.993 ± 0.462

8.303 ± 0.651

5.71 ± 0.735

4.662 ± 0.241

1.628 ± 0.343

3.945 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski