Amino acid dipepetide frequency for Bacillus phage BMBtp1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.249AlaAla: 5.249 ± 1.075
0.09AlaCys: 0.09 ± 0.109
3.529AlaAsp: 3.529 ± 0.605
4.253AlaGlu: 4.253 ± 0.726
2.172AlaPhe: 2.172 ± 0.351
2.715AlaGly: 2.715 ± 0.771
0.814AlaHis: 0.814 ± 0.265
4.344AlaIle: 4.344 ± 0.489
5.158AlaLys: 5.158 ± 0.829
4.796AlaLeu: 4.796 ± 0.704
2.262AlaMet: 2.262 ± 0.488
3.348AlaAsn: 3.348 ± 0.489
1.538AlaPro: 1.538 ± 0.341
2.715AlaGln: 2.715 ± 0.456
3.077AlaArg: 3.077 ± 0.649
3.077AlaSer: 3.077 ± 0.458
3.982AlaThr: 3.982 ± 0.759
3.529AlaVal: 3.529 ± 0.524
1.448AlaTrp: 1.448 ± 0.627
2.896AlaTyr: 2.896 ± 0.537
0.0AlaXaa: 0.0 ± 0.0
Cys
0.271CysAla: 0.271 ± 0.191
0.09CysCys: 0.09 ± 0.1
0.724CysAsp: 0.724 ± 0.266
0.995CysGlu: 0.995 ± 0.313
0.362CysPhe: 0.362 ± 0.193
0.362CysGly: 0.362 ± 0.187
0.362CysHis: 0.362 ± 0.243
0.814CysIle: 0.814 ± 0.281
0.543CysLys: 0.543 ± 0.252
0.362CysLeu: 0.362 ± 0.169
0.452CysMet: 0.452 ± 0.193
0.181CysAsn: 0.181 ± 0.175
0.09CysPro: 0.09 ± 0.1
0.362CysGln: 0.362 ± 0.183
0.452CysArg: 0.452 ± 0.231
0.362CysSer: 0.362 ± 0.184
0.271CysThr: 0.271 ± 0.166
0.271CysVal: 0.271 ± 0.16
0.271CysTrp: 0.271 ± 0.16
0.452CysTyr: 0.452 ± 0.23
0.0CysXaa: 0.0 ± 0.0
Asp
3.167AspAla: 3.167 ± 0.634
0.271AspCys: 0.271 ± 0.136
3.439AspAsp: 3.439 ± 0.673
5.339AspGlu: 5.339 ± 0.827
2.172AspPhe: 2.172 ± 0.388
3.529AspGly: 3.529 ± 0.47
0.724AspHis: 0.724 ± 0.295
4.615AspIle: 4.615 ± 0.66
3.801AspLys: 3.801 ± 0.544
4.344AspLeu: 4.344 ± 0.72
2.353AspMet: 2.353 ± 0.534
3.077AspAsn: 3.077 ± 0.569
2.172AspPro: 2.172 ± 0.442
1.448AspGln: 1.448 ± 0.356
2.715AspArg: 2.715 ± 0.466
2.805AspSer: 2.805 ± 0.398
3.077AspThr: 3.077 ± 0.485
3.891AspVal: 3.891 ± 0.577
1.448AspTrp: 1.448 ± 0.411
2.715AspTyr: 2.715 ± 0.539
0.0AspXaa: 0.0 ± 0.0
Glu
6.063GluAla: 6.063 ± 1.05
1.357GluCys: 1.357 ± 0.461
3.71GluAsp: 3.71 ± 0.464
7.783GluGlu: 7.783 ± 1.045
3.529GluPhe: 3.529 ± 0.435
2.896GluGly: 2.896 ± 0.526
0.905GluHis: 0.905 ± 0.282
6.878GluIle: 6.878 ± 0.831
6.878GluLys: 6.878 ± 0.929
8.054GluLeu: 8.054 ± 0.825
2.353GluMet: 2.353 ± 0.409
4.977GluAsn: 4.977 ± 0.611
2.172GluPro: 2.172 ± 0.508
3.982GluGln: 3.982 ± 0.563
3.077GluArg: 3.077 ± 0.7
3.891GluSer: 3.891 ± 0.532
4.434GluThr: 4.434 ± 0.557
4.253GluVal: 4.253 ± 0.806
0.633GluTrp: 0.633 ± 0.22
2.262GluTyr: 2.262 ± 0.417
0.0GluXaa: 0.0 ± 0.0
Phe
2.534PheAla: 2.534 ± 0.399
0.362PheCys: 0.362 ± 0.171
2.353PheAsp: 2.353 ± 0.408
2.624PheGlu: 2.624 ± 0.54
1.086PhePhe: 1.086 ± 0.339
2.805PheGly: 2.805 ± 0.509
0.633PheHis: 0.633 ± 0.289
2.896PheIle: 2.896 ± 0.495
4.072PheLys: 4.072 ± 0.506
2.262PheLeu: 2.262 ± 0.491
0.543PheMet: 0.543 ± 0.21
2.624PheAsn: 2.624 ± 0.416
1.719PhePro: 1.719 ± 0.367
1.086PheGln: 1.086 ± 0.376
1.448PheArg: 1.448 ± 0.331
2.081PheSer: 2.081 ± 0.54
2.624PheThr: 2.624 ± 0.378
3.801PheVal: 3.801 ± 0.817
0.362PheTrp: 0.362 ± 0.195
1.991PheTyr: 1.991 ± 0.424
0.0PheXaa: 0.0 ± 0.0
Gly
3.982GlyAla: 3.982 ± 0.676
0.362GlyCys: 0.362 ± 0.23
2.624GlyAsp: 2.624 ± 0.415
3.982GlyGlu: 3.982 ± 0.704
3.077GlyPhe: 3.077 ± 0.353
2.986GlyGly: 2.986 ± 0.594
0.995GlyHis: 0.995 ± 0.292
4.796GlyIle: 4.796 ± 0.662
5.701GlyLys: 5.701 ± 0.76
4.706GlyLeu: 4.706 ± 0.766
1.991GlyMet: 1.991 ± 0.442
3.348GlyAsn: 3.348 ± 0.542
0.995GlyPro: 0.995 ± 0.257
2.534GlyGln: 2.534 ± 0.613
2.081GlyArg: 2.081 ± 0.484
2.715GlySer: 2.715 ± 0.463
3.077GlyThr: 3.077 ± 0.512
4.344GlyVal: 4.344 ± 0.568
1.086GlyTrp: 1.086 ± 0.424
2.624GlyTyr: 2.624 ± 0.558
0.0GlyXaa: 0.0 ± 0.0
His
0.905HisAla: 0.905 ± 0.333
0.181HisCys: 0.181 ± 0.2
0.905HisAsp: 0.905 ± 0.301
0.905HisGlu: 0.905 ± 0.259
0.633HisPhe: 0.633 ± 0.246
0.995HisGly: 0.995 ± 0.351
0.271HisHis: 0.271 ± 0.223
1.538HisIle: 1.538 ± 0.344
1.719HisLys: 1.719 ± 0.354
1.81HisLeu: 1.81 ± 0.401
0.09HisMet: 0.09 ± 0.08
0.905HisAsn: 0.905 ± 0.235
0.362HisPro: 0.362 ± 0.188
0.543HisGln: 0.543 ± 0.239
0.452HisArg: 0.452 ± 0.197
0.633HisSer: 0.633 ± 0.186
0.633HisThr: 0.633 ± 0.277
1.448HisVal: 1.448 ± 0.356
0.181HisTrp: 0.181 ± 0.109
0.181HisTyr: 0.181 ± 0.107
0.0HisXaa: 0.0 ± 0.0
Ile
4.344IleAla: 4.344 ± 0.667
0.271IleCys: 0.271 ± 0.169
4.615IleAsp: 4.615 ± 0.49
6.787IleGlu: 6.787 ± 0.966
1.9IlePhe: 1.9 ± 0.489
4.344IleGly: 4.344 ± 0.715
1.357IleHis: 1.357 ± 0.409
4.706IleIle: 4.706 ± 0.691
6.154IleLys: 6.154 ± 0.834
5.249IleLeu: 5.249 ± 0.771
1.81IleMet: 1.81 ± 0.494
3.71IleAsn: 3.71 ± 0.519
3.167IlePro: 3.167 ± 0.537
3.439IleGln: 3.439 ± 0.595
3.167IleArg: 3.167 ± 0.635
4.796IleSer: 4.796 ± 0.733
5.339IleThr: 5.339 ± 0.743
5.701IleVal: 5.701 ± 0.773
0.543IleTrp: 0.543 ± 0.26
3.077IleTyr: 3.077 ± 0.498
0.0IleXaa: 0.0 ± 0.0
Lys
5.973LysAla: 5.973 ± 0.83
0.724LysCys: 0.724 ± 0.257
4.525LysAsp: 4.525 ± 0.516
9.231LysGlu: 9.231 ± 1.115
2.896LysPhe: 2.896 ± 0.488
5.52LysGly: 5.52 ± 0.643
1.538LysHis: 1.538 ± 0.438
6.968LysIle: 6.968 ± 0.849
9.864LysLys: 9.864 ± 0.978
6.697LysLeu: 6.697 ± 0.805
2.896LysMet: 2.896 ± 0.47
5.43LysAsn: 5.43 ± 0.73
1.9LysPro: 1.9 ± 0.468
5.249LysGln: 5.249 ± 0.805
5.249LysArg: 5.249 ± 0.617
4.344LysSer: 4.344 ± 0.673
5.792LysThr: 5.792 ± 0.726
6.063LysVal: 6.063 ± 0.882
0.995LysTrp: 0.995 ± 0.261
3.167LysTyr: 3.167 ± 0.639
0.0LysXaa: 0.0 ± 0.0
Leu
5.249LeuAla: 5.249 ± 0.941
0.633LeuCys: 0.633 ± 0.228
4.706LeuAsp: 4.706 ± 0.738
6.968LeuGlu: 6.968 ± 0.784
3.258LeuPhe: 3.258 ± 0.507
6.154LeuGly: 6.154 ± 1.039
1.81LeuHis: 1.81 ± 0.522
3.439LeuIle: 3.439 ± 0.545
6.516LeuLys: 6.516 ± 0.62
5.158LeuLeu: 5.158 ± 0.668
1.991LeuMet: 1.991 ± 0.419
5.973LeuAsn: 5.973 ± 0.633
1.9LeuPro: 1.9 ± 0.301
2.715LeuGln: 2.715 ± 0.556
3.71LeuArg: 3.71 ± 0.593
5.43LeuSer: 5.43 ± 0.725
3.982LeuThr: 3.982 ± 0.514
4.434LeuVal: 4.434 ± 0.608
1.448LeuTrp: 1.448 ± 0.403
2.353LeuTyr: 2.353 ± 0.59
0.0LeuXaa: 0.0 ± 0.0
Met
1.267MetAla: 1.267 ± 0.339
0.09MetCys: 0.09 ± 0.082
2.081MetAsp: 2.081 ± 0.458
1.719MetGlu: 1.719 ± 0.437
0.814MetPhe: 0.814 ± 0.26
1.448MetGly: 1.448 ± 0.395
0.09MetHis: 0.09 ± 0.09
1.538MetIle: 1.538 ± 0.402
2.805MetLys: 2.805 ± 0.582
2.262MetLeu: 2.262 ± 0.49
0.452MetMet: 0.452 ± 0.173
2.443MetAsn: 2.443 ± 0.515
0.724MetPro: 0.724 ± 0.283
0.271MetGln: 0.271 ± 0.15
1.9MetArg: 1.9 ± 0.448
2.443MetSer: 2.443 ± 0.401
1.719MetThr: 1.719 ± 0.387
2.262MetVal: 2.262 ± 0.426
0.09MetTrp: 0.09 ± 0.084
1.176MetTyr: 1.176 ± 0.489
0.0MetXaa: 0.0 ± 0.0
Asn
3.167AsnAla: 3.167 ± 0.75
0.181AsnCys: 0.181 ± 0.118
3.077AsnAsp: 3.077 ± 0.444
3.982AsnGlu: 3.982 ± 0.588
1.719AsnPhe: 1.719 ± 0.279
5.339AsnGly: 5.339 ± 0.758
0.995AsnHis: 0.995 ± 0.282
4.796AsnIle: 4.796 ± 0.647
6.516AsnLys: 6.516 ± 1.182
4.072AsnLeu: 4.072 ± 0.468
2.172AsnMet: 2.172 ± 0.467
2.534AsnAsn: 2.534 ± 0.537
2.172AsnPro: 2.172 ± 0.486
2.172AsnGln: 2.172 ± 0.341
2.081AsnArg: 2.081 ± 0.523
3.258AsnSer: 3.258 ± 0.597
3.529AsnThr: 3.529 ± 0.684
2.986AsnVal: 2.986 ± 0.57
0.814AsnTrp: 0.814 ± 0.284
2.081AsnTyr: 2.081 ± 0.473
0.0AsnXaa: 0.0 ± 0.0
Pro
0.905ProAla: 0.905 ± 0.289
0.0ProCys: 0.0 ± 0.0
1.991ProAsp: 1.991 ± 0.623
1.538ProGlu: 1.538 ± 0.298
1.267ProPhe: 1.267 ± 0.361
1.086ProGly: 1.086 ± 0.284
0.452ProHis: 0.452 ± 0.219
2.715ProIle: 2.715 ± 0.392
3.71ProLys: 3.71 ± 0.536
3.258ProLeu: 3.258 ± 0.516
0.814ProMet: 0.814 ± 0.267
1.267ProAsn: 1.267 ± 0.352
0.995ProPro: 0.995 ± 0.361
0.724ProGln: 0.724 ± 0.231
0.905ProArg: 0.905 ± 0.286
1.629ProSer: 1.629 ± 0.384
2.353ProThr: 2.353 ± 0.456
2.534ProVal: 2.534 ± 0.576
0.09ProTrp: 0.09 ± 0.089
1.267ProTyr: 1.267 ± 0.369
0.0ProXaa: 0.0 ± 0.0
Gln
3.439GlnAla: 3.439 ± 0.625
0.362GlnCys: 0.362 ± 0.18
1.086GlnAsp: 1.086 ± 0.269
3.439GlnGlu: 3.439 ± 0.513
1.991GlnPhe: 1.991 ± 0.443
2.262GlnGly: 2.262 ± 0.494
0.543GlnHis: 0.543 ± 0.188
3.258GlnIle: 3.258 ± 0.478
4.072GlnLys: 4.072 ± 0.583
3.439GlnLeu: 3.439 ± 0.721
1.357GlnMet: 1.357 ± 0.308
1.991GlnAsn: 1.991 ± 0.4
1.086GlnPro: 1.086 ± 0.277
1.719GlnGln: 1.719 ± 0.419
1.448GlnArg: 1.448 ± 0.341
1.81GlnSer: 1.81 ± 0.397
1.9GlnThr: 1.9 ± 0.376
2.262GlnVal: 2.262 ± 0.511
0.905GlnTrp: 0.905 ± 0.26
1.81GlnTyr: 1.81 ± 0.37
0.0GlnXaa: 0.0 ± 0.0
Arg
1.991ArgAla: 1.991 ± 0.462
0.362ArgCys: 0.362 ± 0.183
2.624ArgAsp: 2.624 ± 0.475
3.62ArgGlu: 3.62 ± 0.67
1.629ArgPhe: 1.629 ± 0.383
2.443ArgGly: 2.443 ± 0.498
0.905ArgHis: 0.905 ± 0.268
3.348ArgIle: 3.348 ± 0.638
3.891ArgLys: 3.891 ± 0.621
2.986ArgLeu: 2.986 ± 0.543
1.538ArgMet: 1.538 ± 0.292
2.534ArgAsn: 2.534 ± 0.422
1.357ArgPro: 1.357 ± 0.328
1.176ArgGln: 1.176 ± 0.347
1.719ArgArg: 1.719 ± 0.428
3.348ArgSer: 3.348 ± 0.564
2.262ArgThr: 2.262 ± 0.485
3.529ArgVal: 3.529 ± 0.516
0.724ArgTrp: 0.724 ± 0.213
1.448ArgTyr: 1.448 ± 0.378
0.0ArgXaa: 0.0 ± 0.0
Ser
3.077SerAla: 3.077 ± 0.643
0.362SerCys: 0.362 ± 0.213
3.258SerAsp: 3.258 ± 0.6
3.62SerGlu: 3.62 ± 0.538
3.077SerPhe: 3.077 ± 0.489
3.529SerGly: 3.529 ± 0.464
0.543SerHis: 0.543 ± 0.208
4.525SerIle: 4.525 ± 0.714
5.701SerLys: 5.701 ± 0.627
4.706SerLeu: 4.706 ± 0.638
0.995SerMet: 0.995 ± 0.332
3.62SerAsn: 3.62 ± 0.71
1.448SerPro: 1.448 ± 0.374
2.353SerGln: 2.353 ± 0.459
2.443SerArg: 2.443 ± 0.51
2.805SerSer: 2.805 ± 0.607
3.982SerThr: 3.982 ± 0.781
3.801SerVal: 3.801 ± 0.617
0.995SerTrp: 0.995 ± 0.378
2.624SerTyr: 2.624 ± 0.425
0.0SerXaa: 0.0 ± 0.0
Thr
4.072ThrAla: 4.072 ± 0.842
0.633ThrCys: 0.633 ± 0.198
4.163ThrAsp: 4.163 ± 0.582
3.167ThrGlu: 3.167 ± 0.605
2.353ThrPhe: 2.353 ± 0.397
3.348ThrGly: 3.348 ± 0.631
0.995ThrHis: 0.995 ± 0.297
4.344ThrIle: 4.344 ± 0.725
4.977ThrLys: 4.977 ± 0.8
4.525ThrLeu: 4.525 ± 0.676
0.995ThrMet: 0.995 ± 0.336
2.896ThrAsn: 2.896 ± 0.633
2.443ThrPro: 2.443 ± 0.536
2.534ThrGln: 2.534 ± 0.532
1.9ThrArg: 1.9 ± 0.425
3.348ThrSer: 3.348 ± 0.574
2.534ThrThr: 2.534 ± 0.488
5.339ThrVal: 5.339 ± 0.605
0.543ThrTrp: 0.543 ± 0.202
1.991ThrTyr: 1.991 ± 0.412
0.0ThrXaa: 0.0 ± 0.0
Val
2.805ValAla: 2.805 ± 0.411
0.905ValCys: 0.905 ± 0.324
3.891ValAsp: 3.891 ± 0.594
5.792ValGlu: 5.792 ± 1.028
3.077ValPhe: 3.077 ± 0.599
3.62ValGly: 3.62 ± 0.633
0.633ValHis: 0.633 ± 0.269
5.158ValIle: 5.158 ± 0.648
7.421ValLys: 7.421 ± 0.688
4.887ValLeu: 4.887 ± 0.587
1.357ValMet: 1.357 ± 0.406
3.529ValAsn: 3.529 ± 0.562
2.172ValPro: 2.172 ± 0.378
2.986ValGln: 2.986 ± 0.52
2.986ValArg: 2.986 ± 0.605
5.249ValSer: 5.249 ± 0.615
3.439ValThr: 3.439 ± 0.57
3.982ValVal: 3.982 ± 0.579
0.724ValTrp: 0.724 ± 0.199
2.986ValTyr: 2.986 ± 0.443
0.0ValXaa: 0.0 ± 0.0
Trp
0.452TrpAla: 0.452 ± 0.245
0.0TrpCys: 0.0 ± 0.0
1.267TrpAsp: 1.267 ± 0.349
1.719TrpGlu: 1.719 ± 0.419
0.543TrpPhe: 0.543 ± 0.202
0.905TrpGly: 0.905 ± 0.359
0.09TrpHis: 0.09 ± 0.081
0.633TrpIle: 0.633 ± 0.215
1.176TrpLys: 1.176 ± 0.256
1.448TrpLeu: 1.448 ± 0.402
0.362TrpMet: 0.362 ± 0.166
0.995TrpAsn: 0.995 ± 0.289
0.181TrpPro: 0.181 ± 0.13
0.271TrpGln: 0.271 ± 0.13
0.452TrpArg: 0.452 ± 0.174
1.357TrpSer: 1.357 ± 0.387
0.724TrpThr: 0.724 ± 0.29
0.543TrpVal: 0.543 ± 0.211
0.09TrpTrp: 0.09 ± 0.094
0.452TrpTyr: 0.452 ± 0.243
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.081TyrAla: 2.081 ± 0.564
0.814TyrCys: 0.814 ± 0.326
2.534TyrAsp: 2.534 ± 0.457
2.624TyrGlu: 2.624 ± 0.595
2.353TyrPhe: 2.353 ± 0.423
1.719TyrGly: 1.719 ± 0.328
0.633TyrHis: 0.633 ± 0.213
3.167TyrIle: 3.167 ± 0.45
4.344TyrLys: 4.344 ± 0.662
2.805TyrLeu: 2.805 ± 0.633
0.724TyrMet: 0.724 ± 0.24
2.262TyrAsn: 2.262 ± 0.406
0.905TyrPro: 0.905 ± 0.386
1.9TyrGln: 1.9 ± 0.348
2.172TyrArg: 2.172 ± 0.439
2.172TyrSer: 2.172 ± 0.387
1.448TyrThr: 1.448 ± 0.38
2.624TyrVal: 2.624 ± 0.442
0.271TyrTrp: 0.271 ± 0.144
1.448TyrTyr: 1.448 ± 0.351
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (11051 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski