Maribacter sp. 4U21

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Maribacter; unclassified Maribacter

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3145 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G5KKG6|A0A2G5KKG6_9FLAO Fis family transcriptional regulator OS=Maribacter sp. 4U21 OX=1889779 GN=BFP77_06105 PE=4 SV=1
MM1 pKa = 7.44LFIASCSTDD10 pKa = 2.91TDD12 pKa = 3.75VLTDD16 pKa = 3.61EE17 pKa = 5.59LLDD20 pKa = 3.86NEE22 pKa = 4.54VTNDD26 pKa = 3.1EE27 pKa = 4.46TASDD31 pKa = 4.1GQNNAPRR38 pKa = 11.84IEE40 pKa = 4.13VTAFQIPEE48 pKa = 3.96HH49 pKa = 5.95STGGTAIGTVTASDD63 pKa = 3.72ADD65 pKa = 3.86EE66 pKa = 4.93DD67 pKa = 4.34RR68 pKa = 11.84LTFTIDD74 pKa = 2.92SNYY77 pKa = 11.06DD78 pKa = 3.33LVIDD82 pKa = 4.4EE83 pKa = 4.64NTGEE87 pKa = 4.09LSVGEE92 pKa = 3.91NLQLDD97 pKa = 4.61FEE99 pKa = 5.19TNNEE103 pKa = 3.79IPFTVSVFDD112 pKa = 4.32GSTIADD118 pKa = 3.36WDD120 pKa = 3.76ATLTITDD127 pKa = 3.32INEE130 pKa = 3.89YY131 pKa = 10.46QILSQAQKK139 pKa = 11.25DD140 pKa = 3.65LVEE143 pKa = 4.51YY144 pKa = 9.35YY145 pKa = 10.57TFLTLRR151 pKa = 11.84KK152 pKa = 8.81SASSPRR158 pKa = 11.84TTNLKK163 pKa = 8.9WGEE166 pKa = 4.2SIKK169 pKa = 10.81LYY171 pKa = 10.57LDD173 pKa = 3.73GAITPAYY180 pKa = 10.23RR181 pKa = 11.84EE182 pKa = 4.14MVTTALAEE190 pKa = 4.13INDD193 pKa = 4.13YY194 pKa = 8.94FTVGDD199 pKa = 3.79FTISLTNNQSDD210 pKa = 4.11ANATLYY216 pKa = 11.04LGDD219 pKa = 3.44VSEE222 pKa = 6.41IEE224 pKa = 4.41TLWPDD229 pKa = 3.03IFDD232 pKa = 3.96IANGSNNQGYY242 pKa = 10.78ASTSFSNNLIDD253 pKa = 6.03DD254 pKa = 3.62ARR256 pKa = 11.84IWLSIDD262 pKa = 3.28SEE264 pKa = 4.24ILFKK268 pKa = 11.17HH269 pKa = 5.57EE270 pKa = 4.71LGHH273 pKa = 6.79GLGLGHH279 pKa = 6.68SNRR282 pKa = 11.84CGSSEE287 pKa = 3.89PVGSFMCSTVSPNHH301 pKa = 6.48NILPSEE307 pKa = 4.34VEE309 pKa = 3.98VLRR312 pKa = 11.84YY313 pKa = 9.54LYY315 pKa = 10.73HH316 pKa = 7.16EE317 pKa = 5.27DD318 pKa = 3.5MPSGLNEE325 pKa = 4.1QEE327 pKa = 4.02IEE329 pKa = 4.32DD330 pKa = 4.0YY331 pKa = 10.98LSNFILLDD339 pKa = 3.43MM340 pKa = 5.59

Molecular weight: 37.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G5KAZ6|A0A2G5KAZ6_9FLAO Pyrroline-5-carboxylate reductase OS=Maribacter sp. 4U21 OX=1889779 GN=proC PE=3 SV=1
MM1 pKa = 7.14NALYY5 pKa = 10.79NLLHH9 pKa = 5.96YY10 pKa = 9.26LQKK13 pKa = 10.67RR14 pKa = 11.84GFEE17 pKa = 4.03VCRR20 pKa = 11.84RR21 pKa = 11.84IAEE24 pKa = 4.14RR25 pKa = 11.84LGIRR29 pKa = 11.84ARR31 pKa = 11.84VVRR34 pKa = 11.84TSFIYY39 pKa = 9.42LTFVTLGFGFALYY52 pKa = 10.63LFLAFWLKK60 pKa = 10.58IKK62 pKa = 10.7DD63 pKa = 3.6LVYY66 pKa = 10.49TKK68 pKa = 9.72RR69 pKa = 11.84TSVFDD74 pKa = 3.66LL75 pKa = 4.18

Molecular weight: 8.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3145

0

3145

1053939

42

2897

335.1

37.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.79 ± 0.042

0.718 ± 0.013

5.711 ± 0.039

6.697 ± 0.043

5.163 ± 0.038

6.786 ± 0.051

1.78 ± 0.021

7.444 ± 0.038

7.448 ± 0.05

9.563 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.3 ± 0.024

5.662 ± 0.046

3.486 ± 0.024

3.273 ± 0.024

3.754 ± 0.027

6.303 ± 0.032

5.745 ± 0.039

6.364 ± 0.036

1.091 ± 0.017

3.921 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski