Paenibacillaceae bacterium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; unclassified Paenibacillaceae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6717 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q2LXI0|A0A4Q2LXI0_9BACL Transcriptional regulator OS=Paenibacillaceae bacterium OX=2003592 GN=EBB07_03410 PE=4 SV=1
MM1 pKa = 7.8LKK3 pKa = 10.23RR4 pKa = 11.84NKK6 pKa = 9.56IKK8 pKa = 10.67LVAVMLMLGALFLAGCSEE26 pKa = 3.85WLEE29 pKa = 4.31EE30 pKa = 4.91NSEE33 pKa = 4.0LWEE36 pKa = 4.08EE37 pKa = 4.65AEE39 pKa = 4.32LQAAADD45 pKa = 3.76NDD47 pKa = 4.03EE48 pKa = 4.41EE49 pKa = 5.04NGLSSVEE56 pKa = 3.87TDD58 pKa = 3.62CYY60 pKa = 10.92VDD62 pKa = 4.37EE63 pKa = 5.55IYY65 pKa = 10.85LSEE68 pKa = 4.23EE69 pKa = 4.09SRR71 pKa = 11.84CITEE75 pKa = 4.05WTCDD79 pKa = 3.68DD80 pKa = 5.52LEE82 pKa = 4.58SCEE85 pKa = 4.1NLGNEE90 pKa = 4.46LMSNLIEE97 pKa = 4.43NYY99 pKa = 10.11GSFVNLEE106 pKa = 3.77EE107 pKa = 4.98SEE109 pKa = 4.09IEE111 pKa = 4.17EE112 pKa = 4.15NEE114 pKa = 4.03KK115 pKa = 10.93LSALEE120 pKa = 4.04EE121 pKa = 4.15QPKK124 pKa = 9.83ASYY127 pKa = 8.89WVEE130 pKa = 3.86EE131 pKa = 4.29EE132 pKa = 4.26EE133 pKa = 5.73LVLEE137 pKa = 5.55DD138 pKa = 4.43GDD140 pKa = 4.06EE141 pKa = 4.54SDD143 pKa = 4.44DD144 pKa = 5.32HH145 pKa = 7.59RR146 pKa = 11.84EE147 pKa = 3.77LWEE150 pKa = 4.37GFAAIIPPHH159 pKa = 5.81EE160 pKa = 4.84RR161 pKa = 11.84EE162 pKa = 4.38DD163 pKa = 3.54VTLFTVFSHH172 pKa = 6.64YY173 pKa = 9.01DD174 pKa = 3.05TLGFVRR180 pKa = 11.84QNDD183 pKa = 3.84DD184 pKa = 5.55DD185 pKa = 4.98YY186 pKa = 11.42STWTFGLNIEE196 pKa = 4.73GAASLNEE203 pKa = 3.77AVNTNIHH210 pKa = 5.96EE211 pKa = 4.71FGHH214 pKa = 6.96LLTLRR219 pKa = 11.84TQQVDD224 pKa = 3.43PDD226 pKa = 4.19AEE228 pKa = 4.01EE229 pKa = 4.52HH230 pKa = 6.48LCNTYY235 pKa = 10.8YY236 pKa = 10.99VDD238 pKa = 4.78DD239 pKa = 4.71GCAEE243 pKa = 3.95SDD245 pKa = 3.29AYY247 pKa = 9.87IYY249 pKa = 11.18AFYY252 pKa = 10.13QQFWSDD258 pKa = 3.63DD259 pKa = 3.71QQDD262 pKa = 3.46EE263 pKa = 4.73DD264 pKa = 3.93EE265 pKa = 5.19DD266 pKa = 4.89SFVSEE271 pKa = 4.22YY272 pKa = 11.37ALTNILEE279 pKa = 5.15DD280 pKa = 3.87IAEE283 pKa = 4.04SWAFFVLADD292 pKa = 4.57RR293 pKa = 11.84PDD295 pKa = 3.69GDD297 pKa = 3.8TVVEE301 pKa = 3.87QKK303 pKa = 10.55ILFFYY308 pKa = 10.3EE309 pKa = 3.86YY310 pKa = 10.69EE311 pKa = 4.1EE312 pKa = 4.16LVKK315 pKa = 10.9LRR317 pKa = 11.84TEE319 pKa = 3.97LLARR323 pKa = 11.84ITSWRR328 pKa = 11.84EE329 pKa = 3.17RR330 pKa = 11.84AASS333 pKa = 3.33

Molecular weight: 38.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q2M2V6|A0A4Q2M2V6_9BACL Gfo/Idh/MocA family oxidoreductase OS=Paenibacillaceae bacterium OX=2003592 GN=EBB07_03175 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PTFRR6 pKa = 11.84PNVSKK11 pKa = 10.72RR12 pKa = 11.84KK13 pKa = 8.96KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 9.99NGRR28 pKa = 11.84KK29 pKa = 8.83VLSARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.26GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6717

0

6717

2160698

19

2710

321.7

35.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.461 ± 0.039

0.765 ± 0.01

5.171 ± 0.025

6.749 ± 0.038

4.15 ± 0.023

7.24 ± 0.033

2.139 ± 0.016

6.756 ± 0.03

5.134 ± 0.03

10.166 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.015

4.0 ± 0.027

3.92 ± 0.017

4.132 ± 0.021

5.104 ± 0.03

6.305 ± 0.023

5.282 ± 0.026

6.842 ± 0.027

1.338 ± 0.013

3.57 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski