Maribacter cobaltidurans

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Maribacter

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4004 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223VA18|A0A223VA18_9FLAO OsmC family peroxiredoxin OS=Maribacter cobaltidurans OX=1178778 GN=CJ263_19445 PE=4 SV=1
MM1 pKa = 7.31AQMTFADD8 pKa = 4.4NFDD11 pKa = 3.49TSDD14 pKa = 3.62YY15 pKa = 11.33DD16 pKa = 3.71RR17 pKa = 11.84QDD19 pKa = 3.1GNTYY23 pKa = 9.36WATDD27 pKa = 4.04WIEE30 pKa = 4.48SDD32 pKa = 4.77DD33 pKa = 4.36SNLGPTSQYY42 pKa = 10.75IRR44 pKa = 11.84IQSQQLYY51 pKa = 8.76MYY53 pKa = 8.47YY54 pKa = 10.62LWTEE58 pKa = 4.67DD59 pKa = 2.97IRR61 pKa = 11.84RR62 pKa = 11.84TANLTGATSATLSFDD77 pKa = 3.34YY78 pKa = 7.9TTNNLGGTRR87 pKa = 11.84RR88 pKa = 11.84LGVYY92 pKa = 10.18ISSTGGAPYY101 pKa = 8.96TQIGTLSGNGTFTQDD116 pKa = 2.05ISAYY120 pKa = 9.13ISANTVIRR128 pKa = 11.84FAKK131 pKa = 10.53SNANWDD137 pKa = 4.01SNHH140 pKa = 6.06NASIDD145 pKa = 3.65NVLISAVTPVDD156 pKa = 3.27TDD158 pKa = 3.21GDD160 pKa = 4.33GIEE163 pKa = 5.48DD164 pKa = 4.57VADD167 pKa = 4.31LDD169 pKa = 4.05NDD171 pKa = 3.48NDD173 pKa = 5.9GILDD177 pKa = 3.54WDD179 pKa = 3.66EE180 pKa = 4.3CTFVATTTQNFNANGGTSVTNTSTSGRR207 pKa = 11.84GVMYY211 pKa = 10.22IDD213 pKa = 6.15FISIDD218 pKa = 3.31NSFNLTVNGTNIAAEE233 pKa = 4.31FQFQPGAPGNFARR246 pKa = 11.84FNTGFTYY253 pKa = 11.01GEE255 pKa = 4.47GGLPQLWSLTGTLANPVLRR274 pKa = 11.84VIVDD278 pKa = 3.55ADD280 pKa = 3.48GHH282 pKa = 6.38LKK284 pKa = 10.83LFGAQSSGGTLEE296 pKa = 4.4PLILDD301 pKa = 4.42TPPEE305 pKa = 4.23TVPWNPSGTNTITIGQFVTGPTNMNGQIRR334 pKa = 11.84FSGEE338 pKa = 3.77CDD340 pKa = 3.09TDD342 pKa = 3.45GDD344 pKa = 4.81GIFNRR349 pKa = 11.84FDD351 pKa = 4.25LDD353 pKa = 3.45SDD355 pKa = 3.62NDD357 pKa = 4.58GIYY360 pKa = 10.79DD361 pKa = 3.63AVEE364 pKa = 4.31AGHH367 pKa = 6.28GQAITNGAINGAVGTDD383 pKa = 3.6GVPNLVQTDD392 pKa = 3.97PNGGSINYY400 pKa = 9.74AYY402 pKa = 9.42TDD404 pKa = 3.49SDD406 pKa = 3.66GDD408 pKa = 4.28GILDD412 pKa = 4.65SIEE415 pKa = 4.76LDD417 pKa = 3.97SDD419 pKa = 3.73NDD421 pKa = 3.63GCNDD425 pKa = 3.14VLEE428 pKa = 5.58AGFTDD433 pKa = 3.68NNADD437 pKa = 3.56GFLGPNPVATDD448 pKa = 3.53GNGVVTSGTDD458 pKa = 3.31GYY460 pKa = 9.1TIPAEE465 pKa = 4.22GDD467 pKa = 3.11LDD469 pKa = 4.24SVFDD473 pKa = 3.9YY474 pKa = 10.5RR475 pKa = 11.84QAGAPPSINTQPVNTIICPGCNGSLSVTATNANLYY510 pKa = 8.28QWQRR514 pKa = 11.84FVGGSWANITDD525 pKa = 3.33NAMFSGANTATLNINNPTPSEE546 pKa = 3.81NGEE549 pKa = 4.01QYY551 pKa = 10.68RR552 pKa = 11.84VVLTNIAYY560 pKa = 10.03SCAQTTSATASLQIRR575 pKa = 11.84VNTIVTNRR583 pKa = 11.84RR584 pKa = 11.84ITYY587 pKa = 9.74RR588 pKa = 11.84VQKK591 pKa = 10.59NN592 pKa = 2.98

Molecular weight: 63.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223V2Y0|A0A223V2Y0_9FLAO 50S ribosomal protein L25 OS=Maribacter cobaltidurans OX=1178778 GN=rplY PE=3 SV=1
MM1 pKa = 7.59QKK3 pKa = 9.07ATWLWRR9 pKa = 11.84VALTRR14 pKa = 11.84YY15 pKa = 9.58AGQEE19 pKa = 3.56LLGAIRR25 pKa = 11.84RR26 pKa = 11.84LFAIAASWEE35 pKa = 4.08KK36 pKa = 11.07CPFFQCGARR45 pKa = 11.84ANFGLFRR52 pKa = 11.84INIGNKK58 pKa = 8.37QSYY61 pKa = 9.49CVAFGGKK68 pKa = 9.12

Molecular weight: 7.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4004

0

4004

1370011

34

4164

342.2

38.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.21 ± 0.036

0.704 ± 0.013

5.758 ± 0.041

6.888 ± 0.048

5.157 ± 0.032

6.836 ± 0.048

1.789 ± 0.021

7.473 ± 0.036

7.412 ± 0.061

9.557 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.383 ± 0.021

5.816 ± 0.037

3.6 ± 0.027

3.355 ± 0.021

3.718 ± 0.026

6.43 ± 0.027

5.58 ± 0.053

6.212 ± 0.034

1.14 ± 0.016

3.983 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski