Streptococcus phage P9853

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Brussowvirus; unclassified Brussowvirus

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286QRR3|A0A286QRR3_9CAUD Antireceptor OS=Streptococcus phage P9853 OX=1971445 GN=P9853_19 PE=4 SV=1
MM1 pKa = 7.13VNWVDD6 pKa = 4.44ANGNDD11 pKa = 4.02LSDD14 pKa = 4.99GADD17 pKa = 3.16QDD19 pKa = 4.45FKK21 pKa = 11.75AGMFFSFAGDD31 pKa = 3.45EE32 pKa = 4.32VNITDD37 pKa = 3.77TGEE40 pKa = 3.65GGYY43 pKa = 10.61YY44 pKa = 9.76GGYY47 pKa = 8.0YY48 pKa = 9.41YY49 pKa = 10.76RR50 pKa = 11.84RR51 pKa = 11.84FEE53 pKa = 4.06FGQFGTVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286QT79|A0A286QT79_9CAUD Uncharacterized protein OS=Streptococcus phage P9853 OX=1971445 GN=P9853_44 PE=4 SV=1
MM1 pKa = 7.25LTFIKK6 pKa = 10.16CLQLFLPKK14 pKa = 10.17KK15 pKa = 7.71QTLRR19 pKa = 11.84GYY21 pKa = 9.2EE22 pKa = 4.1KK23 pKa = 10.16IRR25 pKa = 11.84VFVLGSFITPII36 pKa = 3.64

Molecular weight: 4.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

10676

35

1517

237.2

26.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.746 ± 1.322

0.543 ± 0.146

6.07 ± 0.479

6.248 ± 0.722

4.131 ± 0.161

7.1 ± 0.516

1.208 ± 0.171

6.847 ± 0.414

7.746 ± 0.658

7.559 ± 0.41

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.501 ± 0.317

5.62 ± 0.438

2.96 ± 0.278

3.756 ± 0.246

4.253 ± 0.39

7.119 ± 0.658

6.679 ± 0.349

6.725 ± 0.23

1.218 ± 0.21

3.972 ± 0.499
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski