Amino acid dipepetide frequency for Rhodococcus sp. C1-24

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.538AlaAla: 18.538 ± 0.145	0.931AlaCys: 0.931 ± 0.029	8.639AlaAsp: 8.639 ± 0.084	7.17AlaGlu: 7.17 ± 0.071	3.546AlaPhe: 3.546 ± 0.046	11.205AlaGly: 11.205 ± 0.085	2.475AlaHis: 2.475 ± 0.045	5.763AlaIle: 5.763 ± 0.07	3.413AlaLys: 3.413 ± 0.06	12.845AlaLeu: 12.845 ± 0.102	2.769AlaMet: 2.769 ± 0.045	2.653AlaAsn: 2.653 ± 0.04	5.864AlaPro: 5.864 ± 0.083	3.942AlaGln: 3.942 ± 0.051	8.022AlaArg: 8.022 ± 0.079	6.375AlaSer: 6.375 ± 0.056	7.575AlaThr: 7.575 ± 0.07	11.412AlaVal: 11.412 ± 0.095	1.538AlaTrp: 1.538 ± 0.031	2.328AlaTyr: 2.328 ± 0.036	0.0AlaXaa: 0.0 ± 0.0
Cys	1.009CysAla: 1.009 ± 0.026	0.108CysCys: 0.108 ± 0.008	0.511CysAsp: 0.511 ± 0.018	0.352CysGlu: 0.352 ± 0.015	0.218CysPhe: 0.218 ± 0.013	0.856CysGly: 0.856 ± 0.025	0.185CysHis: 0.185 ± 0.011	0.278CysIle: 0.278 ± 0.012	0.147CysLys: 0.147 ± 0.01	0.614CysLeu: 0.614 ± 0.019	0.112CysMet: 0.112 ± 0.009	0.16CysAsn: 0.16 ± 0.011	0.397CysPro: 0.397 ± 0.016	0.157CysGln: 0.157 ± 0.011	0.488CysArg: 0.488 ± 0.02	0.502CysSer: 0.502 ± 0.018	0.501CysThr: 0.501 ± 0.017	0.623CysVal: 0.623 ± 0.019	0.102CysTrp: 0.102 ± 0.009	0.137CysTyr: 0.137 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	8.225AspAla: 8.225 ± 0.079	0.423AspCys: 0.423 ± 0.017	4.636AspAsp: 4.636 ± 0.06	3.823AspGlu: 3.823 ± 0.054	1.875AspPhe: 1.875 ± 0.036	6.124AspGly: 6.124 ± 0.072	1.493AspHis: 1.493 ± 0.027	2.688AspIle: 2.688 ± 0.04	1.582AspLys: 1.582 ± 0.034	6.5AspLeu: 6.5 ± 0.082	0.969AspMet: 0.969 ± 0.022	1.395AspAsn: 1.395 ± 0.027	4.173AspPro: 4.173 ± 0.056	1.907AspGln: 1.907 ± 0.035	5.123AspArg: 5.123 ± 0.062	3.467AspSer: 3.467 ± 0.053	3.259AspThr: 3.259 ± 0.048	5.836AspVal: 5.836 ± 0.078	0.967AspTrp: 0.967 ± 0.027	1.323AspTyr: 1.323 ± 0.028	0.0AspXaa: 0.0 ± 0.0
Glu	5.332GluAla: 5.332 ± 0.064	0.365GluCys: 0.365 ± 0.014	2.174GluAsp: 2.174 ± 0.039	2.185GluGlu: 2.185 ± 0.043	1.885GluPhe: 1.885 ± 0.037	3.122GluGly: 3.122 ± 0.05	1.548GluHis: 1.548 ± 0.031	2.646GluIle: 2.646 ± 0.045	1.437GluLys: 1.437 ± 0.037	6.644GluLeu: 6.644 ± 0.066	1.008GluMet: 1.008 ± 0.028	1.187GluAsn: 1.187 ± 0.027	2.859GluPro: 2.859 ± 0.051	2.328GluGln: 2.328 ± 0.04	4.529GluArg: 4.529 ± 0.06	3.245GluSer: 3.245 ± 0.047	2.654GluThr: 2.654 ± 0.039	4.135GluVal: 4.135 ± 0.048	0.778GluTrp: 0.778 ± 0.022	1.133GluTyr: 1.133 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	4.238PheAla: 4.238 ± 0.05	0.273PheCys: 0.273 ± 0.013	2.685PheAsp: 2.685 ± 0.05	1.678PheGlu: 1.678 ± 0.035	1.004PhePhe: 1.004 ± 0.024	3.615PheGly: 3.615 ± 0.056	0.657PheHis: 0.657 ± 0.02	1.012PheIle: 1.012 ± 0.026	0.521PheLys: 0.521 ± 0.019	2.595PheLeu: 2.595 ± 0.046	0.48PheMet: 0.48 ± 0.017	0.657PheAsn: 0.657 ± 0.022	1.372PhePro: 1.372 ± 0.028	0.778PheGln: 0.778 ± 0.023	1.807PheArg: 1.807 ± 0.036	1.623PheSer: 1.623 ± 0.034	2.083PheThr: 2.083 ± 0.035	2.783PheVal: 2.783 ± 0.049	0.412PheTrp: 0.412 ± 0.018	0.688PheTyr: 0.688 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	9.917GlyAla: 9.917 ± 0.094	0.797GlyCys: 0.797 ± 0.026	4.956GlyAsp: 4.956 ± 0.057	4.271GlyGlu: 4.271 ± 0.057	3.118GlyPhe: 3.118 ± 0.052	7.568GlyGly: 7.568 ± 0.102	1.887GlyHis: 1.887 ± 0.036	4.467GlyIle: 4.467 ± 0.055	2.724GlyLys: 2.724 ± 0.048	8.545GlyLeu: 8.545 ± 0.084	2.027GlyMet: 2.027 ± 0.031	2.112GlyAsn: 2.112 ± 0.039	4.141GlyPro: 4.141 ± 0.054	2.433GlyGln: 2.433 ± 0.048	6.048GlyArg: 6.048 ± 0.059	5.671GlySer: 5.671 ± 0.058	5.532GlyThr: 5.532 ± 0.065	7.559GlyVal: 7.559 ± 0.057	1.609GlyTrp: 1.609 ± 0.034	2.253GlyTyr: 2.253 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.369HisAla: 2.369 ± 0.042	0.209HisCys: 0.209 ± 0.01	1.421HisAsp: 1.421 ± 0.025	1.028HisGlu: 1.028 ± 0.028	0.664HisPhe: 0.664 ± 0.022	2.048HisGly: 2.048 ± 0.036	0.674HisHis: 0.674 ± 0.022	0.842HisIle: 0.842 ± 0.022	0.372HisLys: 0.372 ± 0.014	2.019HisLeu: 2.019 ± 0.036	0.375HisMet: 0.375 ± 0.014	0.484HisAsn: 0.484 ± 0.015	1.538HisPro: 1.538 ± 0.029	0.628HisGln: 0.628 ± 0.018	1.793HisArg: 1.793 ± 0.034	1.23HisSer: 1.23 ± 0.029	1.137HisThr: 1.137 ± 0.025	1.695HisVal: 1.695 ± 0.03	0.35HisTrp: 0.35 ± 0.015	0.521HisTyr: 0.521 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	7.029IleAla: 7.029 ± 0.072	0.346IleCys: 0.346 ± 0.014	3.97IleAsp: 3.97 ± 0.05	2.79IleGlu: 2.79 ± 0.045	1.16IlePhe: 1.16 ± 0.028	5.08IleGly: 5.08 ± 0.062	0.799IleHis: 0.799 ± 0.023	1.43IleIle: 1.43 ± 0.03	0.915IleLys: 0.915 ± 0.024	3.449IleLeu: 3.449 ± 0.047	0.618IleMet: 0.618 ± 0.02	1.017IleAsn: 1.017 ± 0.026	2.489IlePro: 2.489 ± 0.043	0.883IleGln: 0.883 ± 0.023	2.703IleArg: 2.703 ± 0.044	2.507IleSer: 2.507 ± 0.036	2.833IleThr: 2.833 ± 0.042	4.347IleVal: 4.347 ± 0.056	0.458IleTrp: 0.458 ± 0.015	0.821IleTyr: 0.821 ± 0.022	0.0IleXaa: 0.0 ± 0.0
Lys	2.926LysAla: 2.926 ± 0.053	0.132LysCys: 0.132 ± 0.008	1.361LysAsp: 1.361 ± 0.035	1.044LysGlu: 1.044 ± 0.03	0.753LysPhe: 0.753 ± 0.022	1.725LysGly: 1.725 ± 0.042	0.515LysHis: 0.515 ± 0.017	1.202LysIle: 1.202 ± 0.034	0.839LysLys: 0.839 ± 0.031	2.426LysLeu: 2.426 ± 0.042	0.526LysMet: 0.526 ± 0.017	0.624LysAsn: 0.624 ± 0.022	1.547LysPro: 1.547 ± 0.041	0.844LysGln: 0.844 ± 0.024	1.615LysArg: 1.615 ± 0.038	1.569LysSer: 1.569 ± 0.033	1.583LysThr: 1.583 ± 0.036	2.125LysVal: 2.125 ± 0.04	0.35LysTrp: 0.35 ± 0.015	0.606LysTyr: 0.606 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	13.747LeuAla: 13.747 ± 0.097	0.748LeuCys: 0.748 ± 0.024	7.24LeuAsp: 7.24 ± 0.086	4.42LeuGlu: 4.42 ± 0.056	2.828LeuPhe: 2.828 ± 0.052	8.816LeuGly: 8.816 ± 0.072	2.05LeuHis: 2.05 ± 0.036	4.613LeuIle: 4.613 ± 0.066	1.816LeuLys: 1.816 ± 0.042	9.789LeuLeu: 9.789 ± 0.105	1.634LeuMet: 1.634 ± 0.034	1.955LeuAsn: 1.955 ± 0.038	5.696LeuPro: 5.696 ± 0.065	2.654LeuGln: 2.654 ± 0.04	7.438LeuArg: 7.438 ± 0.081	5.719LeuSer: 5.719 ± 0.064	6.319LeuThr: 6.319 ± 0.072	8.59LeuVal: 8.59 ± 0.076	1.198LeuTrp: 1.198 ± 0.031	1.675LeuTyr: 1.675 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	2.287MetAla: 2.287 ± 0.036	0.154MetCys: 0.154 ± 0.008	0.854MetAsp: 0.854 ± 0.022	0.704MetGlu: 0.704 ± 0.02	0.645MetPhe: 0.645 ± 0.02	1.396MetGly: 1.396 ± 0.029	0.421MetHis: 0.421 ± 0.016	0.932MetIle: 0.932 ± 0.025	0.489MetLys: 0.489 ± 0.018	2.03MetLeu: 2.03 ± 0.036	0.341MetMet: 0.341 ± 0.015	0.523MetAsn: 0.523 ± 0.017	1.141MetPro: 1.141 ± 0.026	0.556MetGln: 0.556 ± 0.02	1.48MetArg: 1.48 ± 0.034	1.616MetSer: 1.616 ± 0.032	1.737MetThr: 1.737 ± 0.033	1.544MetVal: 1.544 ± 0.027	0.251MetTrp: 0.251 ± 0.012	0.343MetTyr: 0.343 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.761AsnAla: 2.761 ± 0.04	0.181AsnCys: 0.181 ± 0.012	1.353AsnAsp: 1.353 ± 0.028	0.998AsnGlu: 0.998 ± 0.028	0.702AsnPhe: 0.702 ± 0.022	2.195AsnGly: 2.195 ± 0.035	0.466AsnHis: 0.466 ± 0.014	0.996AsnIle: 0.996 ± 0.022	0.567AsnLys: 0.567 ± 0.019	2.042AsnLeu: 2.042 ± 0.04	0.437AsnMet: 0.437 ± 0.016	0.605AsnAsn: 0.605 ± 0.02	1.688AsnPro: 1.688 ± 0.033	0.698AsnGln: 0.698 ± 0.025	1.483AsnArg: 1.483 ± 0.031	1.345AsnSer: 1.345 ± 0.03	1.391AsnThr: 1.391 ± 0.032	1.85AsnVal: 1.85 ± 0.036	0.36AsnTrp: 0.36 ± 0.016	0.572AsnTyr: 0.572 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	6.714ProAla: 6.714 ± 0.076	0.266ProCys: 0.266 ± 0.014	4.357ProAsp: 4.357 ± 0.058	3.38ProGlu: 3.38 ± 0.048	1.616ProPhe: 1.616 ± 0.03	4.881ProGly: 4.881 ± 0.057	1.079ProHis: 1.079 ± 0.029	2.575ProIle: 2.575 ± 0.042	1.473ProLys: 1.473 ± 0.032	4.716ProLeu: 4.716 ± 0.06	1.038ProMet: 1.038 ± 0.024	1.442ProAsn: 1.442 ± 0.03	2.832ProPro: 2.832 ± 0.068	1.627ProGln: 1.627 ± 0.036	3.057ProArg: 3.057 ± 0.045	3.092ProSer: 3.092 ± 0.045	3.647ProThr: 3.647 ± 0.052	4.658ProVal: 4.658 ± 0.06	0.747ProTrp: 0.747 ± 0.024	1.043ProTyr: 1.043 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.341GlnAla: 3.341 ± 0.05	0.195GlnCys: 0.195 ± 0.012	1.246GlnAsp: 1.246 ± 0.03	1.139GlnGlu: 1.139 ± 0.025	1.012GlnPhe: 1.012 ± 0.022	1.958GlnGly: 1.958 ± 0.036	0.729GlnHis: 0.729 ± 0.019	1.58GlnIle: 1.58 ± 0.035	0.691GlnLys: 0.691 ± 0.017	3.651GlnLeu: 3.651 ± 0.046	0.607GlnMet: 0.607 ± 0.018	0.657GlnAsn: 0.657 ± 0.022	1.696GlnPro: 1.696 ± 0.036	1.39GlnGln: 1.39 ± 0.039	2.646GlnArg: 2.646 ± 0.041	1.668GlnSer: 1.668 ± 0.032	1.468GlnThr: 1.468 ± 0.03	2.695GlnVal: 2.695 ± 0.044	0.528GlnTrp: 0.528 ± 0.018	0.653GlnTyr: 0.653 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	7.842ArgAla: 7.842 ± 0.085	0.493ArgCys: 0.493 ± 0.017	4.085ArgAsp: 4.085 ± 0.059	3.838ArgGlu: 3.838 ± 0.047	2.384ArgPhe: 2.384 ± 0.04	5.026ArgGly: 5.026 ± 0.063	1.561ArgHis: 1.561 ± 0.032	3.73ArgIle: 3.73 ± 0.043	1.853ArgLys: 1.853 ± 0.037	7.01ArgLeu: 7.01 ± 0.077	1.758ArgMet: 1.758 ± 0.031	1.772ArgAsn: 1.772 ± 0.035	3.588ArgPro: 3.588 ± 0.047	2.062ArgGln: 2.062 ± 0.037	5.994ArgArg: 5.994 ± 0.071	4.447ArgSer: 4.447 ± 0.063	4.596ArgThr: 4.596 ± 0.061	5.467ArgVal: 5.467 ± 0.059	1.229ArgTrp: 1.229 ± 0.029	1.7ArgTyr: 1.7 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	7.381SerAla: 7.381 ± 0.073	0.392SerCys: 0.392 ± 0.017	3.722SerAsp: 3.722 ± 0.054	2.801SerGlu: 2.801 ± 0.037	1.803SerPhe: 1.803 ± 0.038	5.958SerGly: 5.958 ± 0.068	1.042SerHis: 1.042 ± 0.027	2.681SerIle: 2.681 ± 0.043	1.475SerLys: 1.475 ± 0.034	5.222SerLeu: 5.222 ± 0.063	1.468SerMet: 1.468 ± 0.026	1.393SerAsn: 1.393 ± 0.03	2.998SerPro: 2.998 ± 0.04	1.561SerGln: 1.561 ± 0.034	3.763SerArg: 3.763 ± 0.051	3.714SerSer: 3.714 ± 0.051	3.948SerThr: 3.948 ± 0.048	5.041SerVal: 5.041 ± 0.061	0.945SerTrp: 0.945 ± 0.023	1.253SerTyr: 1.253 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	7.933ThrAla: 7.933 ± 0.083	0.401ThrCys: 0.401 ± 0.015	4.139ThrAsp: 4.139 ± 0.051	3.238ThrGlu: 3.238 ± 0.045	1.888ThrPhe: 1.888 ± 0.038	5.797ThrGly: 5.797 ± 0.068	1.202ThrHis: 1.202 ± 0.026	2.808ThrIle: 2.808 ± 0.043	1.451ThrLys: 1.451 ± 0.033	5.745ThrLeu: 5.745 ± 0.06	1.208ThrMet: 1.208 ± 0.026	1.263ThrAsn: 1.263 ± 0.026	3.726ThrPro: 3.726 ± 0.048	1.599ThrGln: 1.599 ± 0.032	3.617ThrArg: 3.617 ± 0.055	3.573ThrSer: 3.573 ± 0.048	4.193ThrThr: 4.193 ± 0.066	5.973ThrVal: 5.973 ± 0.059	0.821ThrTrp: 0.821 ± 0.023	1.318ThrTyr: 1.318 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	11.632ValAla: 11.632 ± 0.102	0.715ValCys: 0.715 ± 0.024	6.244ValAsp: 6.244 ± 0.063	4.734ValGlu: 4.734 ± 0.054	2.591ValPhe: 2.591 ± 0.047	7.32ValGly: 7.32 ± 0.072	1.888ValHis: 1.888 ± 0.03	4.026ValIle: 4.026 ± 0.055	1.752ValLys: 1.752 ± 0.032	9.178ValLeu: 9.178 ± 0.086	1.455ValMet: 1.455 ± 0.027	1.872ValAsn: 1.872 ± 0.034	4.652ValPro: 4.652 ± 0.064	2.231ValGln: 2.231 ± 0.037	5.978ValArg: 5.978 ± 0.067	4.961ValSer: 4.961 ± 0.059	5.417ValThr: 5.417 ± 0.061	8.882ValVal: 8.882 ± 0.093	1.017ValTrp: 1.017 ± 0.027	1.415ValTyr: 1.415 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.471TrpAla: 1.471 ± 0.031	0.132TrpCys: 0.132 ± 0.008	0.788TrpAsp: 0.788 ± 0.022	0.618TrpGlu: 0.618 ± 0.019	0.532TrpPhe: 0.532 ± 0.021	0.989TrpGly: 0.989 ± 0.026	0.323TrpHis: 0.323 ± 0.013	0.69TrpIle: 0.69 ± 0.018	0.383TrpLys: 0.383 ± 0.014	1.657TrpLeu: 1.657 ± 0.041	0.313TrpMet: 0.313 ± 0.013	0.433TrpAsn: 0.433 ± 0.016	0.695TrpPro: 0.695 ± 0.021	0.614TrpGln: 0.614 ± 0.02	1.125TrpArg: 1.125 ± 0.026	1.011TrpSer: 1.011 ± 0.025	0.879TrpThr: 0.879 ± 0.024	1.079TrpVal: 1.079 ± 0.023	0.326TrpTrp: 0.326 ± 0.016	0.284TrpTyr: 0.284 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.312TyrAla: 2.312 ± 0.042	0.193TyrCys: 0.193 ± 0.012	1.407TyrAsp: 1.407 ± 0.03	0.984TyrGlu: 0.984 ± 0.028	0.735TyrPhe: 0.735 ± 0.021	1.957TyrGly: 1.957 ± 0.035	0.401TyrHis: 0.401 ± 0.016	0.683TyrIle: 0.683 ± 0.018	0.412TyrLys: 0.412 ± 0.016	2.268TyrLeu: 2.268 ± 0.037	0.301TyrMet: 0.301 ± 0.013	0.485TyrAsn: 0.485 ± 0.019	1.152TyrPro: 1.152 ± 0.03	0.66TyrGln: 0.66 ± 0.022	1.723TyrArg: 1.723 ± 0.035	1.228TyrSer: 1.228 ± 0.025	1.139TyrThr: 1.139 ± 0.027	1.667TyrVal: 1.667 ± 0.036	0.357TyrTrp: 0.357 ± 0.015	0.48TyrTyr: 0.48 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5215 proteins (1709589 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---