Salvia hispanica RNA virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; unclassified Amalgaviridae

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A386RWS8|A0A386RWS8_9VIRU Coat protein OS=Salvia hispanica RNA virus 1 OX=2419805 PE=4 SV=1
MM1 pKa = 7.26AAEE4 pKa = 4.23APRR7 pKa = 11.84RR8 pKa = 11.84VLAVKK13 pKa = 8.75TPPNYY18 pKa = 10.81DD19 pKa = 3.25EE20 pKa = 4.96MLTNTLAPLAAEE32 pKa = 4.86AFPVHH37 pKa = 5.42TWTRR41 pKa = 11.84AALLANYY48 pKa = 10.23LDD50 pKa = 3.19IKK52 pKa = 10.87RR53 pKa = 11.84FIDD56 pKa = 3.45HH57 pKa = 6.32VKK59 pKa = 10.56VISGEE64 pKa = 3.83NDD66 pKa = 3.24PVIRR70 pKa = 11.84TAMIRR75 pKa = 11.84KK76 pKa = 9.24GNTSAAWNTQGTCTASQMFKK96 pKa = 10.09FCAWLKK102 pKa = 10.57SPEE105 pKa = 4.07GNKK108 pKa = 10.22FITTEE113 pKa = 3.47RR114 pKa = 11.84RR115 pKa = 11.84KK116 pKa = 10.59RR117 pKa = 11.84NIEE120 pKa = 3.48KK121 pKa = 10.38RR122 pKa = 11.84AFEE125 pKa = 4.27GQQFTDD131 pKa = 3.35SSANAAMEE139 pKa = 4.05AQITEE144 pKa = 3.87HH145 pKa = 6.6AMLVKK150 pKa = 10.58KK151 pKa = 10.66SRR153 pKa = 11.84ATTEE157 pKa = 3.73EE158 pKa = 3.85TLLQLRR164 pKa = 11.84RR165 pKa = 11.84EE166 pKa = 4.26IARR169 pKa = 11.84TQQRR173 pKa = 11.84GEE175 pKa = 3.82ADD177 pKa = 3.26IKK179 pKa = 11.24ALEE182 pKa = 4.3ADD184 pKa = 4.72FAPGSAYY191 pKa = 10.79VPMDD195 pKa = 3.98DD196 pKa = 4.01QDD198 pKa = 3.91LGRR201 pKa = 11.84ACHH204 pKa = 5.85QLYY207 pKa = 10.14LAQCQRR213 pKa = 11.84EE214 pKa = 4.17DD215 pKa = 4.5ADD217 pKa = 4.43PEE219 pKa = 4.33DD220 pKa = 3.81LTEE223 pKa = 5.74EE224 pKa = 3.69IMADD228 pKa = 3.11IKK230 pKa = 11.03ATFGSEE236 pKa = 2.94ATARR240 pKa = 11.84HH241 pKa = 5.49RR242 pKa = 11.84AAFIAEE248 pKa = 3.83GHH250 pKa = 6.09RR251 pKa = 11.84RR252 pKa = 11.84QDD254 pKa = 3.4LLAWTEE260 pKa = 3.9AKK262 pKa = 10.12VQQLAGLGDD271 pKa = 4.53LKK273 pKa = 10.93RR274 pKa = 11.84ADD276 pKa = 3.64TFRR279 pKa = 11.84VLVEE283 pKa = 4.09GMGGEE288 pKa = 4.75LSAQEE293 pKa = 3.78RR294 pKa = 11.84AAAEE298 pKa = 4.03ARR300 pKa = 11.84AVAAGGLGPNRR311 pKa = 11.84RR312 pKa = 11.84RR313 pKa = 11.84RR314 pKa = 11.84RR315 pKa = 11.84ADD317 pKa = 3.07VDD319 pKa = 3.39PQNIIDD325 pKa = 3.62HH326 pKa = 5.93PRR328 pKa = 11.84RR329 pKa = 11.84RR330 pKa = 11.84GNQASVLQVEE340 pKa = 5.07APPPPVVQGGDD351 pKa = 3.0AGSRR355 pKa = 11.84AGSPSNPPTDD365 pKa = 3.07IRR367 pKa = 11.84IASPRR372 pKa = 11.84NEE374 pKa = 4.27GGGEE378 pKa = 3.96NPDD381 pKa = 3.36VQEE384 pKa = 4.01

Molecular weight:
42.11 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A386RWS8|A0A386RWS8_9VIRU Coat protein OS=Salvia hispanica RNA virus 1 OX=2419805 PE=4 SV=1
MM1 pKa = 7.26AAEE4 pKa = 4.23APRR7 pKa = 11.84RR8 pKa = 11.84VLAVKK13 pKa = 8.75TPPNYY18 pKa = 10.81DD19 pKa = 3.25EE20 pKa = 4.96MLTNTLAPLAAEE32 pKa = 4.86AFPVHH37 pKa = 5.42TWTRR41 pKa = 11.84AALLANYY48 pKa = 10.23LDD50 pKa = 3.19IKK52 pKa = 10.87RR53 pKa = 11.84FIDD56 pKa = 3.45HH57 pKa = 6.32VKK59 pKa = 10.56VISGEE64 pKa = 3.83NDD66 pKa = 3.24PVIRR70 pKa = 11.84TAMIRR75 pKa = 11.84KK76 pKa = 9.24GNTSAAWNTQGTCTASQMFKK96 pKa = 10.09FCAWLKK102 pKa = 10.57SPEE105 pKa = 4.07GNKK108 pKa = 10.22FITTEE113 pKa = 3.47RR114 pKa = 11.84RR115 pKa = 11.84KK116 pKa = 10.59RR117 pKa = 11.84NIEE120 pKa = 3.48KK121 pKa = 10.38RR122 pKa = 11.84AFEE125 pKa = 4.27GQQFTDD131 pKa = 3.35SSANAAMEE139 pKa = 4.05AQITEE144 pKa = 3.87HH145 pKa = 6.6AMLVKK150 pKa = 10.58KK151 pKa = 10.66SRR153 pKa = 11.84ATTEE157 pKa = 3.73EE158 pKa = 3.85TLLQLRR164 pKa = 11.84RR165 pKa = 11.84EE166 pKa = 4.26IARR169 pKa = 11.84TQQRR173 pKa = 11.84GEE175 pKa = 3.82ADD177 pKa = 3.26IKK179 pKa = 11.24ALEE182 pKa = 4.3ADD184 pKa = 4.72FAPGSAYY191 pKa = 10.79VPMDD195 pKa = 3.98DD196 pKa = 4.01QDD198 pKa = 3.91LGRR201 pKa = 11.84ACHH204 pKa = 5.85QLYY207 pKa = 10.14LAQCQRR213 pKa = 11.84EE214 pKa = 4.17DD215 pKa = 4.5ADD217 pKa = 4.43PEE219 pKa = 4.33DD220 pKa = 3.81LTEE223 pKa = 5.74EE224 pKa = 3.69IMADD228 pKa = 3.11IKK230 pKa = 11.03ATFGSEE236 pKa = 2.94ATARR240 pKa = 11.84HH241 pKa = 5.49RR242 pKa = 11.84AAFIAEE248 pKa = 3.83GHH250 pKa = 6.09RR251 pKa = 11.84RR252 pKa = 11.84QDD254 pKa = 3.4LLAWTEE260 pKa = 3.9AKK262 pKa = 10.12VQQLAGLGDD271 pKa = 4.53LKK273 pKa = 11.02RR274 pKa = 11.84ADD276 pKa = 3.77TFVSSWKK283 pKa = 9.5EE284 pKa = 3.48WAEE287 pKa = 3.92SFLLKK292 pKa = 10.37RR293 pKa = 11.84EE294 pKa = 3.77PRR296 pKa = 11.84LRR298 pKa = 11.84RR299 pKa = 11.84EE300 pKa = 4.12LLQQVVWGPIAAGGGLTSTHH320 pKa = 7.14RR321 pKa = 11.84ISSIIPADD329 pKa = 3.74VVTKK333 pKa = 7.78PQCSKK338 pKa = 10.74SKK340 pKa = 10.24RR341 pKa = 11.84RR342 pKa = 11.84PPLSSKK348 pKa = 10.89VEE350 pKa = 4.16TLDD353 pKa = 3.78LAPGLLVTHH362 pKa = 6.05QRR364 pKa = 11.84TSVLRR369 pKa = 11.84VPAMKK374 pKa = 10.52GGGRR378 pKa = 11.84IPTSRR383 pKa = 11.84SKK385 pKa = 11.24FEE387 pKa = 3.94AKK389 pKa = 9.08VRR391 pKa = 11.84RR392 pKa = 11.84VIGGGEE398 pKa = 3.7MRR400 pKa = 11.84NWAKK404 pKa = 10.74DD405 pKa = 3.29SNMYY409 pKa = 10.39RR410 pKa = 11.84GGGNYY415 pKa = 9.86SDD417 pKa = 4.29ALKK420 pKa = 10.95LLATARR426 pKa = 11.84TDD428 pKa = 3.09IPGSFLHH435 pKa = 6.77KK436 pKa = 10.32HH437 pKa = 4.58FTVNNARR444 pKa = 11.84SFLKK448 pKa = 10.41LPCGLPVPVGPEE460 pKa = 3.83SVRR463 pKa = 11.84MKK465 pKa = 10.82NFNEE469 pKa = 4.09EE470 pKa = 3.78ATAGPSFRR478 pKa = 11.84AFGIYY483 pKa = 9.49RR484 pKa = 11.84KK485 pKa = 9.9KK486 pKa = 10.53GLKK489 pKa = 10.11AEE491 pKa = 4.48LEE493 pKa = 4.16ATVWKK498 pKa = 10.29SLHH501 pKa = 6.57AYY503 pKa = 10.31AEE505 pKa = 4.55GGDD508 pKa = 4.21AEE510 pKa = 4.49SCLPFIAARR519 pKa = 11.84VGYY522 pKa = 9.64RR523 pKa = 11.84SKK525 pKa = 11.42LLTLEE530 pKa = 4.19KK531 pKa = 10.56AFSKK535 pKa = 9.52LTSGEE540 pKa = 4.11CLGRR544 pKa = 11.84CVMMLDD550 pKa = 4.28AFEE553 pKa = 4.2QTYY556 pKa = 11.16SSALYY561 pKa = 10.28NVISGITHH569 pKa = 7.21RR570 pKa = 11.84GRR572 pKa = 11.84HH573 pKa = 5.11NPGSSFRR580 pKa = 11.84NTTVRR585 pKa = 11.84ASSDD589 pKa = 2.37WGMLFEE595 pKa = 4.36EE596 pKa = 4.79VKK598 pKa = 10.53KK599 pKa = 10.82ASAVIEE605 pKa = 4.2LDD607 pKa = 2.83WKK609 pKa = 11.04KK610 pKa = 10.65FDD612 pKa = 4.63RR613 pKa = 11.84DD614 pKa = 3.58RR615 pKa = 11.84PSDD618 pKa = 4.27DD619 pKa = 3.18IYY621 pKa = 11.37FVIDD625 pKa = 4.45IILSCFQPKK634 pKa = 9.97NGYY637 pKa = 7.39EE638 pKa = 3.82ARR640 pKa = 11.84LLEE643 pKa = 3.9AHH645 pKa = 7.23GIMLRR650 pKa = 11.84RR651 pKa = 11.84ALVEE655 pKa = 4.2RR656 pKa = 11.84PFITDD661 pKa = 3.19DD662 pKa = 3.41GGIFTIEE669 pKa = 3.92GMVPSGSLWTGWLDD683 pKa = 3.45TALNTLYY690 pKa = 10.31IKK692 pKa = 10.71AVLFHH697 pKa = 6.76LGFRR701 pKa = 11.84EE702 pKa = 3.93SDD704 pKa = 3.46ASPKK708 pKa = 10.43CAGDD712 pKa = 4.07DD713 pKa = 3.55NLTLIFKK720 pKa = 9.43EE721 pKa = 4.1ATDD724 pKa = 3.75MQLMEE729 pKa = 4.72VKK731 pKa = 10.43RR732 pKa = 11.84LLNEE736 pKa = 4.15WFRR739 pKa = 11.84AGIDD743 pKa = 3.89DD744 pKa = 4.2EE745 pKa = 5.68DD746 pKa = 5.36FMVHH750 pKa = 6.31HH751 pKa = 7.19PPYY754 pKa = 10.05HH755 pKa = 5.67VEE757 pKa = 3.55RR758 pKa = 11.84RR759 pKa = 11.84QAVFPPGTDD768 pKa = 3.28LSKK771 pKa = 10.65GTSKK775 pKa = 11.04LLDD778 pKa = 3.68LAQWVPFEE786 pKa = 5.12DD787 pKa = 4.24PMTIDD792 pKa = 3.27EE793 pKa = 4.82SKK795 pKa = 10.96GLSHH799 pKa = 6.55RR800 pKa = 11.84WKK802 pKa = 10.84YY803 pKa = 10.52IFEE806 pKa = 4.46NKK808 pKa = 9.54PKK810 pKa = 10.38FLSCYY815 pKa = 8.45WDD817 pKa = 3.59EE818 pKa = 4.44NNNPIRR824 pKa = 11.84PAYY827 pKa = 9.63INLEE831 pKa = 3.91KK832 pKa = 10.85LLWPEE837 pKa = 5.22GIHH840 pKa = 5.58EE841 pKa = 4.5TIEE844 pKa = 4.41DD845 pKa = 3.93YY846 pKa = 10.87EE847 pKa = 4.18AALIGMVVDD856 pKa = 4.66NPFNHH861 pKa = 6.71HH862 pKa = 5.73NVNHH866 pKa = 6.29LMHH869 pKa = 7.4RR870 pKa = 11.84YY871 pKa = 10.01CIVQQVKK878 pKa = 10.09RR879 pKa = 11.84MMITGIKK886 pKa = 10.32AEE888 pKa = 4.58DD889 pKa = 3.45VLDD892 pKa = 4.15FCRR895 pKa = 11.84FKK897 pKa = 10.99DD898 pKa = 3.82DD899 pKa = 3.82KK900 pKa = 11.74GEE902 pKa = 4.15GVPFPMVAQWRR913 pKa = 11.84RR914 pKa = 11.84VNGWVDD920 pKa = 3.39MEE922 pKa = 4.62KK923 pKa = 10.88LPFVDD928 pKa = 5.42RR929 pKa = 11.84YY930 pKa = 9.53IAQFRR935 pKa = 11.84DD936 pKa = 3.8FVTGVTSLYY945 pKa = 10.58SRR947 pKa = 11.84SPTGGLDD954 pKa = 2.6SWRR957 pKa = 11.84FMDD960 pKa = 5.26IIRR963 pKa = 11.84GVGNLNDD970 pKa = 3.69AQFGNDD976 pKa = 2.84MVDD979 pKa = 2.16WVTFLKK985 pKa = 10.56GHH987 pKa = 7.31PLTKK991 pKa = 10.16YY992 pKa = 10.1LKK994 pKa = 7.9PTKK997 pKa = 10.16GSRR1000 pKa = 11.84KK1001 pKa = 9.32RR1002 pKa = 11.84KK1003 pKa = 8.52LHH1005 pKa = 6.36SDD1007 pKa = 3.64PEE1009 pKa = 4.36EE1010 pKa = 3.9EE1011 pKa = 3.76ALTRR1015 pKa = 11.84FKK1017 pKa = 11.2VLNHH1021 pKa = 6.07YY1022 pKa = 10.52LDD1024 pKa = 4.08PTWKK1028 pKa = 10.42KK1029 pKa = 11.38KK1030 pKa = 10.17MFDD1033 pKa = 4.05DD1034 pKa = 3.81VDD1036 pKa = 5.22SYY1038 pKa = 12.15ALWISDD1044 pKa = 3.69LLRR1047 pKa = 11.84NKK1049 pKa = 10.47GPTSS1053 pKa = 3.34

Molecular weight:
119.2 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1437

384

1053

718.5

80.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.299 ± 2.001

1.253 ± 0.093

6.193 ± 0.09

6.75 ± 0.468

4.245 ± 0.608

6.611 ± 0.07

2.366 ± 0.239

4.662 ± 0.104

5.776 ± 0.823

8.49 ± 0.757

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.714 ± 0.163

3.688 ± 0.096

5.15 ± 0.255

3.479 ± 0.876

7.794 ± 0.696

5.289 ± 0.609

5.985 ± 0.117

5.289 ± 0.265

1.949 ± 0.399

2.018 ± 0.43

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski