bacterium HR37

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2382 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H6APR4|A0A2H6APR4_9BACT SPOR domain-containing protein OS=bacterium HR37 OX=2035432 GN=HRbin37_02215 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.5ALGYY6 pKa = 10.7LVFCVLIVSGLGCDD20 pKa = 4.17GEE22 pKa = 4.46LEE24 pKa = 4.48SIIEE28 pKa = 4.48GPDD31 pKa = 3.32SKK33 pKa = 10.33ITLEE37 pKa = 4.76DD38 pKa = 4.15ALTQNYY44 pKa = 9.59NLQTPFYY51 pKa = 8.68GIKK54 pKa = 9.41VAIRR58 pKa = 11.84EE59 pKa = 4.21DD60 pKa = 3.47VGLDD64 pKa = 2.86ADD66 pKa = 4.61TIVNLLDD73 pKa = 3.63EE74 pKa = 5.03DD75 pKa = 4.38AVDD78 pKa = 5.83FLDD81 pKa = 4.13CQFEE85 pKa = 4.02RR86 pKa = 11.84GAAIGFEE93 pKa = 4.47EE94 pKa = 4.97FRR96 pKa = 11.84LDD98 pKa = 5.0DD99 pKa = 4.07GTLVPPLSEE108 pKa = 3.53LRR110 pKa = 11.84VFVVPRR116 pKa = 11.84NFEE119 pKa = 3.87CDD121 pKa = 3.13AVDD124 pKa = 4.19RR125 pKa = 11.84DD126 pKa = 3.87VCSGIFYY133 pKa = 10.0PSSDD137 pKa = 4.08LIIIAEE143 pKa = 4.23RR144 pKa = 11.84GIGSCGDD151 pKa = 3.33LSFWKK156 pKa = 10.4HH157 pKa = 4.97EE158 pKa = 4.14LGHH161 pKa = 6.48RR162 pKa = 11.84YY163 pKa = 9.81GMALDD168 pKa = 4.36HH169 pKa = 6.88SNQEE173 pKa = 3.97EE174 pKa = 4.45FEE176 pKa = 4.33PCIDD180 pKa = 4.95PPDD183 pKa = 3.97CFF185 pKa = 4.71

Molecular weight: 20.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H6AIN1|A0A2H6AIN1_9BACT Response regulator protein VraR OS=bacterium HR37 OX=2035432 GN=vraR PE=4 SV=1
MM1 pKa = 7.06QEE3 pKa = 4.1KK4 pKa = 8.78GTVDD8 pKa = 3.47TVNSARR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84LLQIGVYY23 pKa = 10.12SIPAVLLIGTASQARR38 pKa = 11.84TSRR41 pKa = 11.84RR42 pKa = 11.84FGRR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 9.54RR48 pKa = 11.84RR49 pKa = 11.84SFTPGGRR56 pKa = 11.84PTGGGSGLRR65 pKa = 11.84SGRR68 pKa = 11.84GSSFFNRR75 pKa = 11.84FRR77 pKa = 11.84RR78 pKa = 11.84GRR80 pKa = 3.49

Molecular weight: 8.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2382

0

2382

740518

35

1775

310.9

35.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.338 ± 0.049

0.956 ± 0.017

4.733 ± 0.03

8.013 ± 0.06

4.837 ± 0.042

7.422 ± 0.049

1.613 ± 0.02

7.981 ± 0.04

6.9 ± 0.045

10.182 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.015 ± 0.02

3.537 ± 0.028

4.164 ± 0.031

2.256 ± 0.023

6.192 ± 0.041

6.17 ± 0.038

4.618 ± 0.034

7.757 ± 0.049

1.012 ± 0.017

3.304 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski