Amino acid dipepetide frequency for Xanthomonas phage KPhi1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.381AlaAla: 21.381 ± 2.395
1.584AlaCys: 1.584 ± 0.365
7.703AlaAsp: 7.703 ± 0.804
5.471AlaGlu: 5.471 ± 0.702
3.599AlaPhe: 3.599 ± 0.631
9.215AlaGly: 9.215 ± 1.176
2.592AlaHis: 2.592 ± 0.345
5.543AlaIle: 5.543 ± 0.746
4.535AlaLys: 4.535 ± 0.599
12.598AlaLeu: 12.598 ± 1.109
4.247AlaMet: 4.247 ± 0.455
4.103AlaAsn: 4.103 ± 0.599
6.407AlaPro: 6.407 ± 0.586
6.767AlaGln: 6.767 ± 0.865
7.919AlaArg: 7.919 ± 0.845
6.191AlaSer: 6.191 ± 0.705
8.423AlaThr: 8.423 ± 0.943
8.207AlaVal: 8.207 ± 0.71
2.016AlaTrp: 2.016 ± 0.378
2.736AlaTyr: 2.736 ± 0.437
0.072AlaXaa: 0.072 ± 0.076
Cys
1.296CysAla: 1.296 ± 0.399
0.072CysCys: 0.072 ± 0.072
1.008CysAsp: 1.008 ± 0.27
0.504CysGlu: 0.504 ± 0.211
0.216CysPhe: 0.216 ± 0.123
0.936CysGly: 0.936 ± 0.233
0.216CysHis: 0.216 ± 0.106
0.648CysIle: 0.648 ± 0.218
0.216CysLys: 0.216 ± 0.119
0.576CysLeu: 0.576 ± 0.194
0.36CysMet: 0.36 ± 0.168
0.072CysAsn: 0.072 ± 0.066
0.504CysPro: 0.504 ± 0.225
0.432CysGln: 0.432 ± 0.201
1.152CysArg: 1.152 ± 0.251
1.008CysSer: 1.008 ± 0.247
0.0CysThr: 0.0 ± 0.0
0.432CysVal: 0.432 ± 0.17
0.288CysTrp: 0.288 ± 0.179
0.216CysTyr: 0.216 ± 0.125
0.0CysXaa: 0.0 ± 0.0
Asp
8.999AspAla: 8.999 ± 0.893
0.648AspCys: 0.648 ± 0.196
2.304AspAsp: 2.304 ± 0.413
3.24AspGlu: 3.24 ± 0.518
2.088AspPhe: 2.088 ± 0.417
3.959AspGly: 3.959 ± 0.421
1.08AspHis: 1.08 ± 0.301
2.88AspIle: 2.88 ± 0.475
2.16AspLys: 2.16 ± 0.413
6.119AspLeu: 6.119 ± 0.703
1.152AspMet: 1.152 ± 0.334
1.44AspAsn: 1.44 ± 0.349
3.743AspPro: 3.743 ± 0.584
2.232AspGln: 2.232 ± 0.459
3.671AspArg: 3.671 ± 0.483
3.168AspSer: 3.168 ± 0.555
3.168AspThr: 3.168 ± 0.53
3.959AspVal: 3.959 ± 0.448
1.152AspTrp: 1.152 ± 0.336
1.872AspTyr: 1.872 ± 0.348
0.0AspXaa: 0.0 ± 0.0
Glu
6.119GluAla: 6.119 ± 0.807
0.288GluCys: 0.288 ± 0.134
2.736GluAsp: 2.736 ± 0.393
2.16GluGlu: 2.16 ± 0.35
1.656GluPhe: 1.656 ± 0.318
3.527GluGly: 3.527 ± 0.456
0.576GluHis: 0.576 ± 0.178
2.376GluIle: 2.376 ± 0.458
1.368GluLys: 1.368 ± 0.303
5.255GluLeu: 5.255 ± 0.794
1.296GluMet: 1.296 ± 0.249
0.792GluAsn: 0.792 ± 0.306
2.16GluPro: 2.16 ± 0.379
2.304GluGln: 2.304 ± 0.423
4.319GluArg: 4.319 ± 0.54
2.736GluSer: 2.736 ± 0.612
2.592GluThr: 2.592 ± 0.44
3.096GluVal: 3.096 ± 0.453
1.224GluTrp: 1.224 ± 0.262
1.368GluTyr: 1.368 ± 0.311
0.0GluXaa: 0.0 ± 0.0
Phe
3.455PheAla: 3.455 ± 0.468
0.576PheCys: 0.576 ± 0.202
2.232PheAsp: 2.232 ± 0.37
2.232PheGlu: 2.232 ± 0.441
0.72PhePhe: 0.72 ± 0.212
3.096PheGly: 3.096 ± 0.51
0.432PheHis: 0.432 ± 0.238
0.936PheIle: 0.936 ± 0.295
0.72PheLys: 0.72 ± 0.203
1.872PheLeu: 1.872 ± 0.408
0.576PheMet: 0.576 ± 0.193
1.44PheAsn: 1.44 ± 0.356
1.656PhePro: 1.656 ± 0.339
1.152PheGln: 1.152 ± 0.304
2.232PheArg: 2.232 ± 0.405
1.512PheSer: 1.512 ± 0.384
2.232PheThr: 2.232 ± 0.329
3.24PheVal: 3.24 ± 0.443
0.576PheTrp: 0.576 ± 0.186
0.36PheTyr: 0.36 ± 0.178
0.0PheXaa: 0.0 ± 0.0
Gly
8.423GlyAla: 8.423 ± 1.608
1.08GlyCys: 1.08 ± 0.256
3.743GlyAsp: 3.743 ± 0.512
3.383GlyGlu: 3.383 ± 0.439
3.599GlyPhe: 3.599 ± 0.574
9.071GlyGly: 9.071 ± 3.698
1.08GlyHis: 1.08 ± 0.283
3.024GlyIle: 3.024 ± 0.446
2.736GlyLys: 2.736 ± 0.428
6.551GlyLeu: 6.551 ± 0.605
1.656GlyMet: 1.656 ± 0.328
2.52GlyAsn: 2.52 ± 0.497
2.088GlyPro: 2.088 ± 0.43
3.311GlyGln: 3.311 ± 0.574
6.335GlyArg: 6.335 ± 0.634
4.679GlySer: 4.679 ± 0.911
5.255GlyThr: 5.255 ± 0.778
6.623GlyVal: 6.623 ± 0.646
1.008GlyTrp: 1.008 ± 0.227
2.448GlyTyr: 2.448 ± 0.352
0.0GlyXaa: 0.0 ± 0.0
His
1.44HisAla: 1.44 ± 0.291
0.072HisCys: 0.072 ± 0.085
0.648HisAsp: 0.648 ± 0.191
1.008HisGlu: 1.008 ± 0.256
0.72HisPhe: 0.72 ± 0.217
1.728HisGly: 1.728 ± 0.38
0.288HisHis: 0.288 ± 0.13
0.72HisIle: 0.72 ± 0.245
0.504HisLys: 0.504 ± 0.2
1.224HisLeu: 1.224 ± 0.271
0.432HisMet: 0.432 ± 0.154
0.36HisAsn: 0.36 ± 0.183
2.016HisPro: 2.016 ± 0.421
0.144HisGln: 0.144 ± 0.098
1.656HisArg: 1.656 ± 0.356
1.224HisSer: 1.224 ± 0.295
1.224HisThr: 1.224 ± 0.26
2.016HisVal: 2.016 ± 0.462
0.072HisTrp: 0.072 ± 0.067
0.288HisTyr: 0.288 ± 0.15
0.0HisXaa: 0.0 ± 0.0
Ile
5.111IleAla: 5.111 ± 0.68
0.288IleCys: 0.288 ± 0.177
3.959IleAsp: 3.959 ± 0.44
2.736IleGlu: 2.736 ± 0.408
1.008IlePhe: 1.008 ± 0.318
3.527IleGly: 3.527 ± 0.494
0.576IleHis: 0.576 ± 0.218
1.656IleIle: 1.656 ± 0.352
1.656IleLys: 1.656 ± 0.371
3.096IleLeu: 3.096 ± 0.474
0.576IleMet: 0.576 ± 0.207
1.656IleAsn: 1.656 ± 0.379
2.664IlePro: 2.664 ± 0.44
1.728IleGln: 1.728 ± 0.371
2.952IleArg: 2.952 ± 0.445
1.944IleSer: 1.944 ± 0.312
3.383IleThr: 3.383 ± 0.583
2.808IleVal: 2.808 ± 0.561
0.432IleTrp: 0.432 ± 0.157
1.44IleTyr: 1.44 ± 0.382
0.0IleXaa: 0.0 ± 0.0
Lys
4.391LysAla: 4.391 ± 0.714
0.216LysCys: 0.216 ± 0.122
1.44LysAsp: 1.44 ± 0.305
1.872LysGlu: 1.872 ± 0.395
0.792LysPhe: 0.792 ± 0.221
1.368LysGly: 1.368 ± 0.28
0.72LysHis: 0.72 ± 0.207
1.872LysIle: 1.872 ± 0.297
1.224LysLys: 1.224 ± 0.266
3.096LysLeu: 3.096 ± 0.464
0.936LysMet: 0.936 ± 0.318
0.936LysAsn: 0.936 ± 0.257
2.304LysPro: 2.304 ± 0.402
1.656LysGln: 1.656 ± 0.341
1.944LysArg: 1.944 ± 0.416
1.728LysSer: 1.728 ± 0.462
1.512LysThr: 1.512 ± 0.347
2.808LysVal: 2.808 ± 0.473
0.432LysTrp: 0.432 ± 0.158
1.008LysTyr: 1.008 ± 0.223
0.0LysXaa: 0.0 ± 0.0
Leu
10.006LeuAla: 10.006 ± 0.907
0.72LeuCys: 0.72 ± 0.227
5.615LeuAsp: 5.615 ± 0.69
4.895LeuGlu: 4.895 ± 0.707
2.52LeuPhe: 2.52 ± 0.46
5.687LeuGly: 5.687 ± 0.616
1.584LeuHis: 1.584 ± 0.423
3.599LeuIle: 3.599 ± 0.521
2.448LeuLys: 2.448 ± 0.401
5.255LeuLeu: 5.255 ± 0.748
2.592LeuMet: 2.592 ± 0.447
3.024LeuAsn: 3.024 ± 0.454
5.471LeuPro: 5.471 ± 0.75
4.895LeuGln: 4.895 ± 0.761
5.111LeuArg: 5.111 ± 0.618
6.335LeuSer: 6.335 ± 0.923
6.407LeuThr: 6.407 ± 0.754
5.759LeuVal: 5.759 ± 0.734
1.512LeuTrp: 1.512 ± 0.354
1.8LeuTyr: 1.8 ± 0.335
0.0LeuXaa: 0.0 ± 0.0
Met
3.527MetAla: 3.527 ± 0.533
0.504MetCys: 0.504 ± 0.191
1.512MetAsp: 1.512 ± 0.371
0.72MetGlu: 0.72 ± 0.226
0.432MetPhe: 0.432 ± 0.187
1.368MetGly: 1.368 ± 0.325
0.576MetHis: 0.576 ± 0.188
1.152MetIle: 1.152 ± 0.305
0.936MetLys: 0.936 ± 0.292
1.296MetLeu: 1.296 ± 0.287
0.216MetMet: 0.216 ± 0.123
1.152MetAsn: 1.152 ± 0.377
1.08MetPro: 1.08 ± 0.276
1.368MetGln: 1.368 ± 0.305
1.656MetArg: 1.656 ± 0.363
2.088MetSer: 2.088 ± 0.353
2.304MetThr: 2.304 ± 0.265
1.224MetVal: 1.224 ± 0.244
0.504MetTrp: 0.504 ± 0.155
0.504MetTyr: 0.504 ± 0.165
0.0MetXaa: 0.0 ± 0.0
Asn
4.463AsnAla: 4.463 ± 0.576
0.576AsnCys: 0.576 ± 0.187
1.728AsnAsp: 1.728 ± 0.359
1.008AsnGlu: 1.008 ± 0.302
1.152AsnPhe: 1.152 ± 0.273
3.527AsnGly: 3.527 ± 0.638
0.576AsnHis: 0.576 ± 0.271
1.008AsnIle: 1.008 ± 0.353
1.152AsnLys: 1.152 ± 0.308
2.808AsnLeu: 2.808 ± 0.396
0.648AsnMet: 0.648 ± 0.208
1.224AsnAsn: 1.224 ± 0.257
2.376AsnPro: 2.376 ± 0.407
1.152AsnGln: 1.152 ± 0.259
2.736AsnArg: 2.736 ± 0.405
1.512AsnSer: 1.512 ± 0.315
2.232AsnThr: 2.232 ± 0.32
1.944AsnVal: 1.944 ± 0.413
0.648AsnTrp: 0.648 ± 0.168
0.936AsnTyr: 0.936 ± 0.313
0.0AsnXaa: 0.0 ± 0.0
Pro
6.839ProAla: 6.839 ± 0.888
0.144ProCys: 0.144 ± 0.124
4.031ProAsp: 4.031 ± 0.58
3.743ProGlu: 3.743 ± 0.587
2.088ProPhe: 2.088 ± 0.398
4.607ProGly: 4.607 ± 0.733
0.864ProHis: 0.864 ± 0.332
2.664ProIle: 2.664 ± 0.466
1.008ProLys: 1.008 ± 0.292
4.103ProLeu: 4.103 ± 0.599
1.44ProMet: 1.44 ± 0.291
2.232ProAsn: 2.232 ± 0.444
2.664ProPro: 2.664 ± 0.635
2.736ProGln: 2.736 ± 0.453
2.52ProArg: 2.52 ± 0.376
3.311ProSer: 3.311 ± 0.422
4.607ProThr: 4.607 ± 0.735
3.743ProVal: 3.743 ± 0.474
0.648ProTrp: 0.648 ± 0.231
1.44ProTyr: 1.44 ± 0.309
0.0ProXaa: 0.0 ± 0.0
Gln
6.695GlnAla: 6.695 ± 0.82
0.504GlnCys: 0.504 ± 0.223
2.304GlnAsp: 2.304 ± 0.447
1.512GlnGlu: 1.512 ± 0.407
1.584GlnPhe: 1.584 ± 0.367
2.52GlnGly: 2.52 ± 0.413
1.152GlnHis: 1.152 ± 0.38
2.88GlnIle: 2.88 ± 0.512
1.008GlnLys: 1.008 ± 0.274
4.535GlnLeu: 4.535 ± 0.543
0.648GlnMet: 0.648 ± 0.241
1.152GlnAsn: 1.152 ± 0.313
2.232GlnPro: 2.232 ± 0.348
3.096GlnGln: 3.096 ± 0.68
3.096GlnArg: 3.096 ± 0.548
3.168GlnSer: 3.168 ± 0.534
2.808GlnThr: 2.808 ± 0.452
3.383GlnVal: 3.383 ± 0.459
0.792GlnTrp: 0.792 ± 0.272
1.296GlnTyr: 1.296 ± 0.304
0.0GlnXaa: 0.0 ± 0.0
Arg
7.991ArgAla: 7.991 ± 0.907
0.648ArgCys: 0.648 ± 0.167
4.247ArgAsp: 4.247 ± 0.581
3.671ArgGlu: 3.671 ± 0.537
2.16ArgPhe: 2.16 ± 0.412
4.463ArgGly: 4.463 ± 0.483
1.152ArgHis: 1.152 ± 0.242
2.088ArgIle: 2.088 ± 0.364
2.736ArgLys: 2.736 ± 0.647
6.191ArgLeu: 6.191 ± 0.838
2.16ArgMet: 2.16 ± 0.397
1.872ArgAsn: 1.872 ± 0.407
3.168ArgPro: 3.168 ± 0.542
3.383ArgGln: 3.383 ± 0.613
4.751ArgArg: 4.751 ± 0.713
4.319ArgSer: 4.319 ± 0.808
3.168ArgThr: 3.168 ± 0.471
3.887ArgVal: 3.887 ± 0.533
1.512ArgTrp: 1.512 ± 0.352
2.376ArgTyr: 2.376 ± 0.344
0.0ArgXaa: 0.0 ± 0.0
Ser
8.135SerAla: 8.135 ± 1.233
0.36SerCys: 0.36 ± 0.171
3.311SerAsp: 3.311 ± 0.473
2.592SerGlu: 2.592 ± 0.43
1.872SerPhe: 1.872 ± 0.402
5.759SerGly: 5.759 ± 0.92
1.08SerHis: 1.08 ± 0.369
3.096SerIle: 3.096 ± 0.553
2.304SerLys: 2.304 ± 0.374
4.823SerLeu: 4.823 ± 0.781
1.008SerMet: 1.008 ± 0.251
2.16SerAsn: 2.16 ± 0.345
3.455SerPro: 3.455 ± 0.49
2.304SerGln: 2.304 ± 0.327
2.736SerArg: 2.736 ± 0.414
2.808SerSer: 2.808 ± 0.443
4.103SerThr: 4.103 ± 0.679
4.391SerVal: 4.391 ± 0.618
1.152SerTrp: 1.152 ± 0.427
1.224SerTyr: 1.224 ± 0.292
0.072SerXaa: 0.072 ± 0.073
Thr
8.639ThrAla: 8.639 ± 1.012
0.36ThrCys: 0.36 ± 0.16
3.599ThrAsp: 3.599 ± 0.48
2.088ThrGlu: 2.088 ± 0.368
1.512ThrPhe: 1.512 ± 0.313
5.687ThrGly: 5.687 ± 0.936
1.44ThrHis: 1.44 ± 0.259
2.952ThrIle: 2.952 ± 0.497
1.872ThrLys: 1.872 ± 0.354
6.263ThrLeu: 6.263 ± 0.873
1.512ThrMet: 1.512 ± 0.363
2.16ThrAsn: 2.16 ± 0.368
4.391ThrPro: 4.391 ± 0.579
2.664ThrGln: 2.664 ± 0.436
2.88ThrArg: 2.88 ± 0.491
3.455ThrSer: 3.455 ± 0.532
3.887ThrThr: 3.887 ± 0.953
5.471ThrVal: 5.471 ± 0.777
1.368ThrTrp: 1.368 ± 0.305
1.872ThrTyr: 1.872 ± 0.499
0.0ThrXaa: 0.0 ± 0.0
Val
9.575ValAla: 9.575 ± 0.896
0.792ValCys: 0.792 ± 0.22
4.247ValAsp: 4.247 ± 0.59
3.311ValGlu: 3.311 ± 0.358
2.232ValPhe: 2.232 ± 0.522
5.111ValGly: 5.111 ± 0.674
0.936ValHis: 0.936 ± 0.223
2.808ValIle: 2.808 ± 0.409
2.592ValLys: 2.592 ± 0.489
5.615ValLeu: 5.615 ± 0.624
2.016ValMet: 2.016 ± 0.363
2.952ValAsn: 2.952 ± 0.472
4.535ValPro: 4.535 ± 0.708
2.736ValGln: 2.736 ± 0.418
5.111ValArg: 5.111 ± 0.682
4.175ValSer: 4.175 ± 0.602
4.247ValThr: 4.247 ± 0.665
5.039ValVal: 5.039 ± 0.658
1.224ValTrp: 1.224 ± 0.283
1.944ValTyr: 1.944 ± 0.4
0.0ValXaa: 0.0 ± 0.0
Trp
2.016TrpAla: 2.016 ± 0.44
0.36TrpCys: 0.36 ± 0.156
0.864TrpAsp: 0.864 ± 0.273
1.152TrpGlu: 1.152 ± 0.261
0.36TrpPhe: 0.36 ± 0.144
0.864TrpGly: 0.864 ± 0.222
0.288TrpHis: 0.288 ± 0.156
0.504TrpIle: 0.504 ± 0.232
0.504TrpLys: 0.504 ± 0.165
1.656TrpLeu: 1.656 ± 0.338
0.288TrpMet: 0.288 ± 0.142
0.792TrpAsn: 0.792 ± 0.23
0.936TrpPro: 0.936 ± 0.254
0.936TrpGln: 0.936 ± 0.251
1.224TrpArg: 1.224 ± 0.318
1.728TrpSer: 1.728 ± 0.362
1.008TrpThr: 1.008 ± 0.302
0.864TrpVal: 0.864 ± 0.261
0.504TrpTrp: 0.504 ± 0.182
0.648TrpTyr: 0.648 ± 0.207
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.808TyrAla: 2.808 ± 0.478
0.36TyrCys: 0.36 ± 0.142
1.8TyrAsp: 1.8 ± 0.299
0.648TyrGlu: 0.648 ± 0.275
0.864TyrPhe: 0.864 ± 0.272
2.592TyrGly: 2.592 ± 0.374
0.576TyrHis: 0.576 ± 0.197
0.792TyrIle: 0.792 ± 0.194
0.576TyrLys: 0.576 ± 0.181
2.304TyrLeu: 2.304 ± 0.499
0.288TyrMet: 0.288 ± 0.14
1.368TyrAsn: 1.368 ± 0.384
1.584TyrPro: 1.584 ± 0.385
1.296TyrGln: 1.296 ± 0.266
2.016TyrArg: 2.016 ± 0.333
1.656TyrSer: 1.656 ± 0.354
1.512TyrThr: 1.512 ± 0.314
2.304TyrVal: 2.304 ± 0.484
0.432TyrTrp: 0.432 ± 0.197
0.576TyrTyr: 0.576 ± 0.177
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.072XaaPhe: 0.072 ± 0.073
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.072XaaGln: 0.072 ± 0.076
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (13892 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski