Streptomyces phage Darolandstone

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Raleighvirus; Streptomyces virus Darolandstone

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386KKQ5|A0A386KKQ5_9CAUD Uncharacterized protein OS=Streptomyces phage Darolandstone OX=2315716 GN=47 PE=4 SV=1
MM1 pKa = 7.05FVEE4 pKa = 4.97FFPVVVTALPADD16 pKa = 4.35EE17 pKa = 5.28DD18 pKa = 4.14HH19 pKa = 7.31APLLVDD25 pKa = 3.83PAAARR30 pKa = 11.84IVRR33 pKa = 11.84AEE35 pKa = 3.85QVVEE39 pKa = 3.91GDD41 pKa = 3.49TVLASFVAYY50 pKa = 9.95GKK52 pKa = 10.07RR53 pKa = 11.84LPVADD58 pKa = 4.52YY59 pKa = 11.23FNDD62 pKa = 3.31QYY64 pKa = 10.56TARR67 pKa = 11.84PKK69 pKa = 10.45TYY71 pKa = 10.46DD72 pKa = 3.48PTCGCGSCATMADD85 pKa = 3.47HH86 pKa = 7.2EE87 pKa = 5.08GPVVDD92 pKa = 6.13LGDD95 pKa = 4.33DD96 pKa = 4.07NPWDD100 pKa = 3.87VCDD103 pKa = 4.17PWPAADD109 pKa = 4.7LVLIVPAAA117 pKa = 3.53

Molecular weight: 12.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386KLG2|A0A386KLG2_9CAUD MuF-like minor capsid protein OS=Streptomyces phage Darolandstone OX=2315716 GN=4 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 5.45RR3 pKa = 11.84PTPAARR9 pKa = 11.84FAAKK13 pKa = 10.13VNPAGPWSLRR23 pKa = 11.84RR24 pKa = 11.84DD25 pKa = 4.04CPGPCHH31 pKa = 6.82LWDD34 pKa = 4.1GAQNEE39 pKa = 4.44KK40 pKa = 10.9GYY42 pKa = 8.79GTFWVAGRR50 pKa = 11.84TVKK53 pKa = 10.35AHH55 pKa = 6.7RR56 pKa = 11.84YY57 pKa = 9.42AYY59 pKa = 8.9EE60 pKa = 3.88QALGPIPAGLEE71 pKa = 3.57VDD73 pKa = 3.36HH74 pKa = 6.83RR75 pKa = 11.84CRR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84EE80 pKa = 4.09CVAPGHH86 pKa = 6.61LEE88 pKa = 4.11AVTHH92 pKa = 6.0RR93 pKa = 11.84VNVLRR98 pKa = 11.84STNHH102 pKa = 4.33VAQRR106 pKa = 11.84AAVTHH111 pKa = 6.5CPAGHH116 pKa = 7.36AYY118 pKa = 10.08DD119 pKa = 3.49QANTIRR125 pKa = 11.84AKK127 pKa = 10.82DD128 pKa = 3.55GTRR131 pKa = 11.84KK132 pKa = 9.49CRR134 pKa = 11.84ACKK137 pKa = 9.94NAAARR142 pKa = 11.84AARR145 pKa = 11.84ARR147 pKa = 11.84ARR149 pKa = 11.84EE150 pKa = 4.02DD151 pKa = 2.9RR152 pKa = 11.84LAPVAPLRR160 pKa = 11.84PRR162 pKa = 11.84PTRR165 pKa = 11.84TLEE168 pKa = 3.81RR169 pKa = 11.84AAA171 pKa = 4.4

Molecular weight: 18.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12910

56

748

234.7

25.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.213 ± 0.51

0.728 ± 0.125

6.909 ± 0.364

5.848 ± 0.407

1.929 ± 0.138

8.9 ± 0.543

2.099 ± 0.205

3.122 ± 0.241

2.215 ± 0.23

8.675 ± 0.484

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.65 ± 0.132

1.882 ± 0.138

6.646 ± 0.28

3.904 ± 0.387

8.861 ± 0.592

4.198 ± 0.23

6.483 ± 0.429

6.917 ± 0.331

1.789 ± 0.144

2.029 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski