Streptococcus phage Javan536

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BEU3|A0A4D6BEU3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan536 OX=2548249 GN=Javan536_0008 PE=4 SV=1
MM1 pKa = 7.17TSKK4 pKa = 8.78TQLLNTLEE12 pKa = 4.22SLVNQRR18 pKa = 11.84VTVPTNPYY26 pKa = 9.94GGQCAALIDD35 pKa = 4.29YY36 pKa = 8.32VLQYY40 pKa = 11.52AGLFNLDD47 pKa = 3.78FSYY50 pKa = 11.08MNAIDD55 pKa = 4.08GLDD58 pKa = 3.27RR59 pKa = 11.84AEE61 pKa = 4.02NLGLKK66 pKa = 7.36VTRR69 pKa = 11.84FNGSNNPPVGSVWVTNCLPYY89 pKa = 10.51HH90 pKa = 5.36QFGHH94 pKa = 6.24IGFVVAEE101 pKa = 4.33NPDD104 pKa = 3.56GTVTTVEE111 pKa = 3.96QNIDD115 pKa = 3.34GNADD119 pKa = 3.43CLYY122 pKa = 10.67NGGWTRR128 pKa = 11.84KK129 pKa = 5.87VTRR132 pKa = 11.84NLDD135 pKa = 3.39SAGNFSYY142 pKa = 10.63IDD144 pKa = 3.24WSAPSQQMVGWFEE157 pKa = 4.62LPFDD161 pKa = 4.39GMTEE165 pKa = 3.7NNYY168 pKa = 10.45FIDD171 pKa = 3.6VSAYY175 pKa = 9.87QPGDD179 pKa = 3.36LTSICSASGTNNTVIKK195 pKa = 9.37VTEE198 pKa = 4.14GVGWVSPVATQQTNTSNCIGYY219 pKa = 7.92YY220 pKa = 9.85HH221 pKa = 7.1FARR224 pKa = 11.84FGGDD228 pKa = 2.81VATAQAEE235 pKa = 4.18ANYY238 pKa = 9.8FISNLPSHH246 pKa = 6.82PRR248 pKa = 11.84YY249 pKa = 9.38LVCDD253 pKa = 4.07YY254 pKa = 11.32EE255 pKa = 6.54DD256 pKa = 4.01GASGDD261 pKa = 3.62KK262 pKa = 10.06QANTNAVLAFMDD274 pKa = 3.3ICKK277 pKa = 10.71ANGFEE282 pKa = 4.51PIYY285 pKa = 10.66YY286 pKa = 9.81SYY288 pKa = 11.36KK289 pKa = 10.34PYY291 pKa = 10.33TLANVYY297 pKa = 9.91VDD299 pKa = 4.33QITARR304 pKa = 11.84YY305 pKa = 7.8PNSLWIAAYY314 pKa = 8.94PDD316 pKa = 3.74YY317 pKa = 10.64EE318 pKa = 4.52VRR320 pKa = 11.84PEE322 pKa = 4.38PYY324 pKa = 9.07WGVYY328 pKa = 10.28PNMEE332 pKa = 4.23HH333 pKa = 5.47TRR335 pKa = 11.84WWQFTSTGLAGGLDD349 pKa = 3.76KK350 pKa = 11.39NVVIINDD357 pKa = 3.56GDD359 pKa = 4.01SLVNKK364 pKa = 9.9EE365 pKa = 4.19KK366 pKa = 11.01EE367 pKa = 4.39EE368 pKa = 4.09EE369 pKa = 4.06NMDD372 pKa = 3.59YY373 pKa = 10.92VVRR376 pKa = 11.84SEE378 pKa = 4.67SGSQGYY384 pKa = 10.02VGVVNGRR391 pKa = 11.84VFGIGSMGTVDD402 pKa = 3.97ALRR405 pKa = 11.84SAGAKK410 pKa = 10.07HH411 pKa = 5.78LTLPDD416 pKa = 3.46ADD418 pKa = 3.99FDD420 pKa = 4.53RR421 pKa = 11.84FLNSQSNDD429 pKa = 3.02TQAVAKK435 pKa = 10.01AVSEE439 pKa = 4.22ASASVVKK446 pKa = 10.68AIEE449 pKa = 3.89EE450 pKa = 4.05RR451 pKa = 11.84AQATQGQTGVV461 pKa = 3.31

Molecular weight: 50.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BEX6|A0A4D6BEX6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan536 OX=2548249 GN=Javan536_0014 PE=4 SV=1
MM1 pKa = 7.79IVIRR5 pKa = 11.84HH6 pKa = 4.96NAKK9 pKa = 10.45VIEE12 pKa = 4.43GQVAVLNGTQYY23 pKa = 11.38DD24 pKa = 3.86IVRR27 pKa = 11.84VSPNEE32 pKa = 3.62NFGLNRR38 pKa = 11.84YY39 pKa = 9.61DD40 pKa = 4.26FLTLRR45 pKa = 11.84KK46 pKa = 8.91HH47 pKa = 6.08KK48 pKa = 10.69KK49 pKa = 9.55VGG51 pKa = 3.25

Molecular weight: 5.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

11581

47

1654

269.3

30.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.046 ± 0.596

0.518 ± 0.12

6.364 ± 0.255

6.606 ± 0.538

4.076 ± 0.191

7.193 ± 0.527

1.2 ± 0.121

5.725 ± 0.261

8.013 ± 0.491

7.745 ± 0.388

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.34 ± 0.187

6.398 ± 0.321

3.488 ± 0.415

4.404 ± 0.242

3.877 ± 0.305

6.407 ± 0.308

7.098 ± 0.396

6.39 ± 0.241

1.382 ± 0.153

3.73 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski