Candidatus Nitrosocaldus cavascurensis

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Nitrososphaeria; Candidatus Nitrosocaldales; Candidatus Nitrosocaldaceae; Candidatus Nitrosocaldus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1766 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K5ARG9|A0A2K5ARG9_9ARCH Uncharacterized protein OS=Candidatus Nitrosocaldus cavascurensis OX=2058097 GN=NCAV_1022 PE=4 SV=1
MM1 pKa = 7.78AEE3 pKa = 3.87NGGITRR9 pKa = 11.84AEE11 pKa = 4.01KK12 pKa = 10.42RR13 pKa = 11.84VTSATAITITITTILLLMVVSMTVTDD39 pKa = 5.21GIRR42 pKa = 11.84TVYY45 pKa = 10.18AQSATITAEE54 pKa = 4.47CNGHH58 pKa = 5.0TIEE61 pKa = 5.45RR62 pKa = 11.84GNFDD66 pKa = 4.19ADD68 pKa = 3.27GDD70 pKa = 4.36DD71 pKa = 3.69EE72 pKa = 5.05VKK74 pKa = 10.83VDD76 pKa = 3.41GTVYY80 pKa = 11.06NDD82 pKa = 3.56GDD84 pKa = 4.65TITLPSGTYY93 pKa = 8.81TIRR96 pKa = 11.84IATPSWPTPYY106 pKa = 10.76NGTDD110 pKa = 3.37DD111 pKa = 3.87NDD113 pKa = 4.78FIVGTSGDD121 pKa = 3.71DD122 pKa = 3.6YY123 pKa = 11.33IFGRR127 pKa = 11.84GEE129 pKa = 4.01DD130 pKa = 3.7DD131 pKa = 4.47FIVGFGGNDD140 pKa = 3.38YY141 pKa = 11.09LYY143 pKa = 10.95GDD145 pKa = 4.08VVKK148 pKa = 9.88GTDD151 pKa = 3.72GADD154 pKa = 3.36TLEE157 pKa = 4.71GGDD160 pKa = 6.15DD161 pKa = 3.67ILCGNDD167 pKa = 3.23GDD169 pKa = 4.66DD170 pKa = 3.75YY171 pKa = 11.58LYY173 pKa = 11.4GDD175 pKa = 4.61VIGGGNGNDD184 pKa = 3.32TMTGGNDD191 pKa = 3.1TLYY194 pKa = 11.01GGKK197 pKa = 10.79GNDD200 pKa = 3.3NLFGDD205 pKa = 5.46GIGGAYY211 pKa = 10.15GDD213 pKa = 4.26DD214 pKa = 3.83TLTGGDD220 pKa = 3.44DD221 pKa = 3.32TLYY224 pKa = 11.06GGEE227 pKa = 4.16GDD229 pKa = 4.71DD230 pKa = 3.67WLEE233 pKa = 3.98GDD235 pKa = 4.67VIGGGDD241 pKa = 4.28GDD243 pKa = 3.71NTMTGGNDD251 pKa = 3.07TLYY254 pKa = 10.85GGKK257 pKa = 10.51GSDD260 pKa = 2.98RR261 pKa = 11.84LYY263 pKa = 11.46GDD265 pKa = 5.19GIWGEE270 pKa = 4.59DD271 pKa = 3.74GDD273 pKa = 4.15NTMTGGNDD281 pKa = 3.07TLYY284 pKa = 10.85GGKK287 pKa = 10.51GSDD290 pKa = 2.98RR291 pKa = 11.84LYY293 pKa = 11.27GDD295 pKa = 4.82GIGGGVGNDD304 pKa = 3.45TLTGGDD310 pKa = 3.81DD311 pKa = 3.63TLKK314 pKa = 11.24GGDD317 pKa = 4.05GDD319 pKa = 4.38DD320 pKa = 3.8YY321 pKa = 11.61LYY323 pKa = 11.26GDD325 pKa = 5.18SIDD328 pKa = 4.47GGPGVDD334 pKa = 5.11KK335 pKa = 10.96IQGGNDD341 pKa = 3.06TLYY344 pKa = 11.01GGKK347 pKa = 10.74GNDD350 pKa = 3.41KK351 pKa = 10.98LFGDD355 pKa = 5.47GIWGDD360 pKa = 3.72ADD362 pKa = 3.94NDD364 pKa = 3.95TAYY367 pKa = 10.69GGNDD371 pKa = 3.35VIDD374 pKa = 4.03ARR376 pKa = 11.84DD377 pKa = 3.6NVEE380 pKa = 5.08DD381 pKa = 4.13NDD383 pKa = 5.44SIDD386 pKa = 3.25GDD388 pKa = 4.3YY389 pKa = 10.75IILAEE394 pKa = 4.29STTEE398 pKa = 4.07GNQDD402 pKa = 2.62ICASDD407 pKa = 4.02PEE409 pKa = 4.33DD410 pKa = 3.63TEE412 pKa = 4.95VNCEE416 pKa = 3.81YY417 pKa = 11.06HH418 pKa = 7.41DD419 pKa = 4.01ISGLATLTTDD429 pKa = 3.62SATIPLGGSVQIIFNSSVDD448 pKa = 3.75NPVKK452 pKa = 9.74ITNLRR457 pKa = 11.84VITPTGNTCTYY468 pKa = 9.78TGTLPIIVPSNPFTATYY485 pKa = 9.22PDD487 pKa = 3.72NFIDD491 pKa = 4.18IDD493 pKa = 3.79TGTDD497 pKa = 3.38CDD499 pKa = 3.73TSAVGEE505 pKa = 4.28YY506 pKa = 9.81TVVAEE511 pKa = 4.63TEE513 pKa = 4.06VGDD516 pKa = 5.26PITTNFNISFNVVPEE531 pKa = 4.11AVVGAVGMVGTGFLSILLYY550 pKa = 10.4RR551 pKa = 11.84RR552 pKa = 11.84LRR554 pKa = 11.84GKK556 pKa = 9.14SRR558 pKa = 11.84KK559 pKa = 7.93EE560 pKa = 3.55

Molecular weight: 58.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K5ASG8|A0A2K5ASG8_9ARCH Uncharacterized protein OS=Candidatus Nitrosocaldus cavascurensis OX=2058097 GN=NCAV_1432 PE=4 SV=1
MM1 pKa = 7.81GSRR4 pKa = 11.84KK5 pKa = 7.97TKK7 pKa = 8.99PRR9 pKa = 11.84KK10 pKa = 8.81NRR12 pKa = 11.84LMKK15 pKa = 9.39KK16 pKa = 7.91TKK18 pKa = 10.09QNSPVPLWVILKK30 pKa = 7.0TNRR33 pKa = 11.84RR34 pKa = 11.84VRR36 pKa = 11.84RR37 pKa = 11.84NPQQRR42 pKa = 11.84HH43 pKa = 3.83WRR45 pKa = 11.84RR46 pKa = 11.84SSVDD50 pKa = 3.09VGG52 pKa = 3.49

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1766

0

1766

461185

24

1803

261.1

29.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.613 ± 0.059

1.06 ± 0.024

6.09 ± 0.061

6.57 ± 0.074

3.18 ± 0.04

6.909 ± 0.064

1.976 ± 0.025

8.286 ± 0.052

5.683 ± 0.058

9.21 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.435 ± 0.036

4.111 ± 0.044

3.365 ± 0.04

1.994 ± 0.034

6.462 ± 0.061

7.021 ± 0.069

4.302 ± 0.057

7.809 ± 0.052

0.884 ± 0.021

4.04 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski