Actibacterium mucosum KCTC 23349

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Actibacterium; Actibacterium mucosum

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3616 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A037ZFG2|A0A037ZFG2_9RHOB Phosphonates import ATP-binding protein PhnC OS=Actibacterium mucosum KCTC 23349 OX=1454373 GN=phnC PE=3 SV=1
MM1 pKa = 7.78KK2 pKa = 9.82STTFTAKK9 pKa = 10.29RR10 pKa = 11.84AGPEE14 pKa = 3.81GARR17 pKa = 11.84TNEE20 pKa = 3.45VMFRR24 pKa = 11.84DD25 pKa = 4.26GGLDD29 pKa = 3.38PLNAFSPLLPVDD41 pKa = 5.21DD42 pKa = 5.44LFANQWYY49 pKa = 9.85LLNTGQSGGVPGVDD63 pKa = 3.53LNVVDD68 pKa = 4.37VWDD71 pKa = 5.18DD72 pKa = 3.56YY73 pKa = 10.85TGEE76 pKa = 4.2GVTVGIWDD84 pKa = 4.62DD85 pKa = 3.74GVQYY89 pKa = 9.02THH91 pKa = 7.41HH92 pKa = 7.5DD93 pKa = 3.44LDD95 pKa = 4.77DD96 pKa = 4.8NYY98 pKa = 11.56DD99 pKa = 3.86EE100 pKa = 4.86NLHH103 pKa = 5.49ITVGGFVHH111 pKa = 7.36DD112 pKa = 4.75PAPQAFDD119 pKa = 3.7SAHH122 pKa = 5.21GTSVAGIIGAEE133 pKa = 4.1NNGEE137 pKa = 4.18GTVGVAYY144 pKa = 10.09DD145 pKa = 3.47ATLAGVDD152 pKa = 3.18IFFDD156 pKa = 4.08FFLNFEE162 pKa = 4.03QSFYY166 pKa = 11.27EE167 pKa = 4.23LDD169 pKa = 3.59NFDD172 pKa = 3.96VTNHH176 pKa = 4.32SWGWSSPYY184 pKa = 10.24LDD186 pKa = 4.91SIYY189 pKa = 10.11DD190 pKa = 3.62TSGSSGTDD198 pKa = 2.64WQTFFGGIQQSVITGRR214 pKa = 11.84DD215 pKa = 3.18GLGTINVVANGNDD228 pKa = 3.46RR229 pKa = 11.84EE230 pKa = 4.18IGRR233 pKa = 11.84DD234 pKa = 3.73GNDD237 pKa = 3.19SNLNNIPQTIAVGATSHH254 pKa = 6.35EE255 pKa = 4.6GFVSYY260 pKa = 11.12YY261 pKa = 8.42STPGANLLISAPSNGEE277 pKa = 3.93PGSGVYY283 pKa = 7.51TTDD286 pKa = 2.93RR287 pKa = 11.84TGFSGYY293 pKa = 10.24EE294 pKa = 3.81LGDD297 pKa = 3.56YY298 pKa = 10.44TSDD301 pKa = 3.76FGGTSSAAPAVAGVVALMLEE321 pKa = 4.43ANPDD325 pKa = 3.7LGWRR329 pKa = 11.84DD330 pKa = 3.49VQEE333 pKa = 4.17ILAVTARR340 pKa = 11.84HH341 pKa = 5.66TGSDD345 pKa = 2.96IGAGPSFEE353 pKa = 5.14EE354 pKa = 3.8LHH356 pKa = 5.27TWEE359 pKa = 5.4FNGATNWNGGGLHH372 pKa = 6.85FSNDD376 pKa = 3.45YY377 pKa = 11.18GFGLIDD383 pKa = 3.58ALAAVRR389 pKa = 11.84LAEE392 pKa = 4.17TWTEE396 pKa = 4.07QQTSANWEE404 pKa = 4.14TPIVGSQTLNTFIQDD419 pKa = 3.58GNPNGISFTFEE430 pKa = 3.41VTNAFDD436 pKa = 4.08LEE438 pKa = 4.67HH439 pKa = 6.86VGLTLDD445 pKa = 4.07FASGRR450 pKa = 11.84TGDD453 pKa = 3.65YY454 pKa = 10.43TIVLVSPDD462 pKa = 3.39GTRR465 pKa = 11.84STLAVSNTGDD475 pKa = 3.36AFTDD479 pKa = 2.88EE480 pKa = 4.4WFYY483 pKa = 10.9MSNEE487 pKa = 3.71FRR489 pKa = 11.84GEE491 pKa = 4.18TGVGTWTVEE500 pKa = 3.99IVDD503 pKa = 3.79EE504 pKa = 4.22RR505 pKa = 11.84LGVSGILEE513 pKa = 4.06SAEE516 pKa = 3.78LQFFGHH522 pKa = 6.31SADD525 pKa = 4.29ANDD528 pKa = 3.71TYY530 pKa = 11.62VYY532 pKa = 10.19TNEE535 pKa = 4.07FSDD538 pKa = 3.98FAGDD542 pKa = 3.93GNHH545 pKa = 5.2VTVLQDD551 pKa = 3.25TNGGVDD557 pKa = 3.73TLNAAAVTADD567 pKa = 3.42AFVFLNFGAFIDD579 pKa = 3.83STFISGVTGIEE590 pKa = 3.74NVYY593 pKa = 10.02TGDD596 pKa = 3.35GNDD599 pKa = 2.97RR600 pKa = 11.84VFGNNDD606 pKa = 3.82DD607 pKa = 3.74NTLWGGRR614 pKa = 11.84GNDD617 pKa = 3.91QISGGQGSDD626 pKa = 3.26TLVDD630 pKa = 3.65GGGTDD635 pKa = 3.59VLIGGAGGDD644 pKa = 3.5IYY646 pKa = 11.58SLGVDD651 pKa = 3.41TAFDD655 pKa = 4.24IIFGFADD662 pKa = 3.91GFDD665 pKa = 4.95FIQFEE670 pKa = 4.5AEE672 pKa = 4.0DD673 pKa = 3.7VSYY676 pKa = 11.61EE677 pKa = 4.0DD678 pKa = 5.31LSVFNLNAGRR688 pKa = 11.84VLVQYY693 pKa = 10.2QGEE696 pKa = 4.47SVLVIEE702 pKa = 5.49AGLTAADD709 pKa = 3.66FDD711 pKa = 4.17EE712 pKa = 6.56ADD714 pKa = 3.65FLFAA718 pKa = 6.2

Molecular weight: 76.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A037ZGX6|A0A037ZGX6_9RHOB NAD(P) transhydrogenase subunit alpha OS=Actibacterium mucosum KCTC 23349 OX=1454373 GN=ACMU_13570 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.5ILNARR34 pKa = 11.84RR35 pKa = 11.84LRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3616

0

3616

1123337

41

2766

310.7

33.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.5 ± 0.059

0.844 ± 0.013

6.222 ± 0.039

5.597 ± 0.04

3.91 ± 0.026

8.708 ± 0.035

2.055 ± 0.019

5.094 ± 0.03

3.143 ± 0.032

10.016 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.786 ± 0.02

2.852 ± 0.019

5.057 ± 0.028

3.412 ± 0.027

6.289 ± 0.038

4.959 ± 0.027

5.541 ± 0.025

7.465 ± 0.033

1.377 ± 0.015

2.174 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski