Leucoagaricus sp. SymC.cos

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Agaricaceae; Leucoagaricus; unclassified Leucoagaricus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12747 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A137QRF3|A0A137QRF3_9AGAR Vacuolar amino acid transporter 1 OS=Leucoagaricus sp. SymC.cos OX=1714833 GN=AN958_04869 PE=3 SV=1
MM1 pKa = 7.47NSFFKK6 pKa = 10.58TLIALSLLAPGHH18 pKa = 6.92LFTLAGPVKK27 pKa = 10.23RR28 pKa = 11.84QDD30 pKa = 4.04DD31 pKa = 5.05GISTDD36 pKa = 3.55ATSTTPLASSQLASSTPTFPSDD58 pKa = 2.99SDD60 pKa = 3.83TFSVPTASPSDD71 pKa = 4.03FPSDD75 pKa = 3.53PTSTPSCSCQMTDD88 pKa = 3.42MNPIPVFSSGTPVDD102 pKa = 4.25PPAPTDD108 pKa = 3.34TMIPTWSDD116 pKa = 2.98GSHH119 pKa = 5.93CHH121 pKa = 5.68HH122 pKa = 7.2HH123 pKa = 6.86GGMPNPSDD131 pKa = 3.95GFPGGLGGDD140 pKa = 3.49NSTPTGDD147 pKa = 3.0IPYY150 pKa = 9.37PGGAMPTPPSTLTAVSVPFPSDD172 pKa = 3.22TSPAPPSTATEE183 pKa = 4.03VGVPAFSDD191 pKa = 3.66SSAEE195 pKa = 3.94PTPLSTATEE204 pKa = 4.16VGTPYY209 pKa = 10.57PGDD212 pKa = 3.89ASPTPPSTLTAVSITDD228 pKa = 3.53PTDD231 pKa = 3.23VPEE234 pKa = 4.28TSGGEE239 pKa = 3.99STAIPTSSTVTDD251 pKa = 4.16SEE253 pKa = 4.86DD254 pKa = 3.46PAPTSTT260 pKa = 4.5

Molecular weight: 26.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A137QIR8|A0A137QIR8_9AGAR von Willebrand factor A domain-containing protein 8 OS=Leucoagaricus sp. SymC.cos OX=1714833 GN=AN958_09143 PE=4 SV=1
MM1 pKa = 7.79TIRR4 pKa = 11.84PRR6 pKa = 11.84PRR8 pKa = 11.84TGTGMVYY15 pKa = 10.32RR16 pKa = 11.84KK17 pKa = 10.01SNSGSFTPNLAGIGAGPGPGPSAGVGTGGSRR48 pKa = 11.84IRR50 pKa = 11.84APMPLATASFTSVTMNTGSGGSVGSVVGKK79 pKa = 10.04SGTMRR84 pKa = 11.84NVSSGLAVRR93 pKa = 11.84RR94 pKa = 11.84ARR96 pKa = 11.84TPGPRR101 pKa = 11.84SASSPSPSPSSSSMPPPPSSMSATPIGQAFF131 pKa = 3.41

Molecular weight: 12.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12747

0

12747

5393887

16

4977

423.1

46.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.709 ± 0.019

1.268 ± 0.01

5.454 ± 0.014

5.904 ± 0.021

3.91 ± 0.016

6.396 ± 0.025

2.586 ± 0.01

5.262 ± 0.017

4.851 ± 0.019

9.346 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.042 ± 0.008

3.791 ± 0.012

6.376 ± 0.03

3.838 ± 0.013

5.85 ± 0.017

8.596 ± 0.032

6.089 ± 0.016

6.316 ± 0.016

1.462 ± 0.008

2.745 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski