Amino acid dipepetide frequency for Mycobacterium phage Hades

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.793AlaAla: 14.793 ± 1.929
1.134AlaCys: 1.134 ± 0.272
6.802AlaAsp: 6.802 ± 0.561
8.219AlaGlu: 8.219 ± 0.869
2.777AlaPhe: 2.777 ± 0.459
9.409AlaGly: 9.409 ± 1.115
2.154AlaHis: 2.154 ± 0.348
4.874AlaIle: 4.874 ± 0.747
3.457AlaLys: 3.457 ± 0.346
8.445AlaLeu: 8.445 ± 0.79
2.324AlaMet: 2.324 ± 0.386
2.721AlaAsn: 2.721 ± 0.396
4.704AlaPro: 4.704 ± 0.572
3.287AlaGln: 3.287 ± 0.392
8.105AlaArg: 8.105 ± 0.979
4.704AlaSer: 4.704 ± 0.593
6.178AlaThr: 6.178 ± 0.53
6.291AlaVal: 6.291 ± 0.662
2.947AlaTrp: 2.947 ± 0.536
2.381AlaTyr: 2.381 ± 0.399
0.0AlaXaa: 0.0 ± 0.0
Cys
0.907CysAla: 0.907 ± 0.274
0.0CysCys: 0.0 ± 0.0
1.02CysAsp: 1.02 ± 0.323
0.737CysGlu: 0.737 ± 0.205
0.227CysPhe: 0.227 ± 0.11
1.7CysGly: 1.7 ± 0.36
0.17CysHis: 0.17 ± 0.093
0.057CysIle: 0.057 ± 0.059
0.34CysLys: 0.34 ± 0.135
0.794CysLeu: 0.794 ± 0.228
0.113CysMet: 0.113 ± 0.081
0.51CysAsn: 0.51 ± 0.172
1.134CysPro: 1.134 ± 0.33
0.34CysGln: 0.34 ± 0.136
0.567CysArg: 0.567 ± 0.197
0.85CysSer: 0.85 ± 0.269
0.68CysThr: 0.68 ± 0.239
0.68CysVal: 0.68 ± 0.187
0.283CysTrp: 0.283 ± 0.113
0.227CysTyr: 0.227 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
6.802AspAla: 6.802 ± 0.684
0.623AspCys: 0.623 ± 0.177
4.478AspAsp: 4.478 ± 0.615
3.231AspGlu: 3.231 ± 0.375
1.87AspPhe: 1.87 ± 0.296
6.178AspGly: 6.178 ± 0.616
1.247AspHis: 1.247 ± 0.22
2.494AspIle: 2.494 ± 0.421
1.814AspLys: 1.814 ± 0.324
6.065AspLeu: 6.065 ± 0.579
1.134AspMet: 1.134 ± 0.267
1.757AspAsn: 1.757 ± 0.331
4.818AspPro: 4.818 ± 0.517
2.607AspGln: 2.607 ± 0.381
4.534AspArg: 4.534 ± 0.576
3.457AspSer: 3.457 ± 0.457
4.251AspThr: 4.251 ± 0.595
4.421AspVal: 4.421 ± 0.574
1.304AspTrp: 1.304 ± 0.267
1.757AspTyr: 1.757 ± 0.353
0.0AspXaa: 0.0 ± 0.0
Glu
5.101GluAla: 5.101 ± 0.59
0.794GluCys: 0.794 ± 0.312
2.891GluAsp: 2.891 ± 0.374
2.891GluGlu: 2.891 ± 0.507
2.437GluPhe: 2.437 ± 0.354
3.117GluGly: 3.117 ± 0.342
1.417GluHis: 1.417 ± 0.394
1.984GluIle: 1.984 ± 0.324
2.211GluLys: 2.211 ± 0.281
4.988GluLeu: 4.988 ± 0.699
1.7GluMet: 1.7 ± 0.275
1.927GluAsn: 1.927 ± 0.312
3.117GluPro: 3.117 ± 0.453
3.174GluGln: 3.174 ± 0.42
5.044GluArg: 5.044 ± 0.685
3.174GluSer: 3.174 ± 0.644
4.704GluThr: 4.704 ± 0.586
4.138GluVal: 4.138 ± 0.538
1.644GluTrp: 1.644 ± 0.279
1.304GluTyr: 1.304 ± 0.279
0.0GluXaa: 0.0 ± 0.0
Phe
2.721PheAla: 2.721 ± 0.44
0.283PheCys: 0.283 ± 0.138
2.381PheAsp: 2.381 ± 0.5
1.587PheGlu: 1.587 ± 0.297
0.68PhePhe: 0.68 ± 0.22
2.891PheGly: 2.891 ± 0.732
0.51PheHis: 0.51 ± 0.146
1.36PheIle: 1.36 ± 0.334
0.794PheLys: 0.794 ± 0.219
2.154PheLeu: 2.154 ± 0.341
0.964PheMet: 0.964 ± 0.29
1.077PheAsn: 1.077 ± 0.333
1.644PhePro: 1.644 ± 0.328
1.077PheGln: 1.077 ± 0.321
1.474PheArg: 1.474 ± 0.232
1.36PheSer: 1.36 ± 0.276
2.267PheThr: 2.267 ± 0.351
2.211PheVal: 2.211 ± 0.3
0.68PheTrp: 0.68 ± 0.178
0.85PheTyr: 0.85 ± 0.248
0.0PheXaa: 0.0 ± 0.0
Gly
9.069GlyAla: 9.069 ± 1.158
1.02GlyCys: 1.02 ± 0.277
5.895GlyAsp: 5.895 ± 0.552
3.571GlyGlu: 3.571 ± 0.477
2.777GlyPhe: 2.777 ± 0.428
11.959GlyGly: 11.959 ± 2.096
1.7GlyHis: 1.7 ± 0.26
4.251GlyIle: 4.251 ± 0.656
2.664GlyLys: 2.664 ± 0.384
5.555GlyLeu: 5.555 ± 0.545
2.947GlyMet: 2.947 ± 0.537
3.457GlyAsn: 3.457 ± 0.394
4.421GlyPro: 4.421 ± 0.52
2.267GlyGln: 2.267 ± 0.568
5.328GlyArg: 5.328 ± 0.648
6.575GlySer: 6.575 ± 0.938
6.461GlyThr: 6.461 ± 0.862
5.668GlyVal: 5.668 ± 0.601
2.154GlyTrp: 2.154 ± 0.344
2.211GlyTyr: 2.211 ± 0.402
0.0GlyXaa: 0.0 ± 0.0
His
1.87HisAla: 1.87 ± 0.29
0.34HisCys: 0.34 ± 0.181
1.36HisAsp: 1.36 ± 0.236
1.02HisGlu: 1.02 ± 0.256
0.453HisPhe: 0.453 ± 0.144
1.7HisGly: 1.7 ± 0.314
0.737HisHis: 0.737 ± 0.247
1.36HisIle: 1.36 ± 0.299
0.737HisLys: 0.737 ± 0.234
1.53HisLeu: 1.53 ± 0.3
0.623HisMet: 0.623 ± 0.162
0.907HisAsn: 0.907 ± 0.215
1.417HisPro: 1.417 ± 0.292
0.85HisGln: 0.85 ± 0.218
2.097HisArg: 2.097 ± 0.401
1.077HisSer: 1.077 ± 0.231
1.474HisThr: 1.474 ± 0.361
1.474HisVal: 1.474 ± 0.298
0.453HisTrp: 0.453 ± 0.158
0.737HisTyr: 0.737 ± 0.201
0.0HisXaa: 0.0 ± 0.0
Ile
4.818IleAla: 4.818 ± 0.606
0.68IleCys: 0.68 ± 0.256
3.798IleAsp: 3.798 ± 0.509
3.287IleGlu: 3.287 ± 0.401
0.623IlePhe: 0.623 ± 0.134
3.854IleGly: 3.854 ± 0.494
1.53IleHis: 1.53 ± 0.319
1.417IleIle: 1.417 ± 0.323
1.02IleLys: 1.02 ± 0.219
1.814IleLeu: 1.814 ± 0.383
0.453IleMet: 0.453 ± 0.17
1.927IleAsn: 1.927 ± 0.318
2.834IlePro: 2.834 ± 0.324
1.587IleGln: 1.587 ± 0.259
3.004IleArg: 3.004 ± 0.513
2.211IleSer: 2.211 ± 0.498
4.421IleThr: 4.421 ± 0.542
3.117IleVal: 3.117 ± 0.401
0.85IleTrp: 0.85 ± 0.19
0.85IleTyr: 0.85 ± 0.192
0.0IleXaa: 0.0 ± 0.0
Lys
3.628LysAla: 3.628 ± 0.524
0.34LysCys: 0.34 ± 0.125
1.474LysAsp: 1.474 ± 0.292
1.417LysGlu: 1.417 ± 0.327
1.417LysPhe: 1.417 ± 0.226
2.494LysGly: 2.494 ± 0.358
1.134LysHis: 1.134 ± 0.266
0.907LysIle: 0.907 ± 0.233
1.7LysLys: 1.7 ± 0.422
2.437LysLeu: 2.437 ± 0.485
0.794LysMet: 0.794 ± 0.198
0.907LysAsn: 0.907 ± 0.252
2.154LysPro: 2.154 ± 0.367
1.53LysGln: 1.53 ± 0.272
2.437LysArg: 2.437 ± 0.344
2.154LysSer: 2.154 ± 0.291
1.927LysThr: 1.927 ± 0.303
2.664LysVal: 2.664 ± 0.362
1.077LysTrp: 1.077 ± 0.277
1.02LysTyr: 1.02 ± 0.261
0.0LysXaa: 0.0 ± 0.0
Leu
8.049LeuAla: 8.049 ± 0.675
0.567LeuCys: 0.567 ± 0.208
4.818LeuAsp: 4.818 ± 0.646
4.081LeuGlu: 4.081 ± 0.484
2.154LeuPhe: 2.154 ± 0.297
5.044LeuGly: 5.044 ± 0.458
1.19LeuHis: 1.19 ± 0.305
3.004LeuIle: 3.004 ± 0.388
1.87LeuLys: 1.87 ± 0.358
4.308LeuLeu: 4.308 ± 0.512
1.814LeuMet: 1.814 ± 0.266
2.664LeuAsn: 2.664 ± 0.374
5.271LeuPro: 5.271 ± 0.568
2.664LeuGln: 2.664 ± 0.395
4.874LeuArg: 4.874 ± 0.677
5.044LeuSer: 5.044 ± 0.504
5.441LeuThr: 5.441 ± 0.545
4.874LeuVal: 4.874 ± 0.461
1.36LeuTrp: 1.36 ± 0.308
1.984LeuTyr: 1.984 ± 0.378
0.0LeuXaa: 0.0 ± 0.0
Met
2.437MetAla: 2.437 ± 0.411
0.283MetCys: 0.283 ± 0.174
1.304MetAsp: 1.304 ± 0.252
1.02MetGlu: 1.02 ± 0.207
0.737MetPhe: 0.737 ± 0.226
1.587MetGly: 1.587 ± 0.263
0.113MetHis: 0.113 ± 0.092
0.68MetIle: 0.68 ± 0.206
0.907MetLys: 0.907 ± 0.249
1.757MetLeu: 1.757 ± 0.258
0.51MetMet: 0.51 ± 0.216
0.964MetAsn: 0.964 ± 0.214
1.247MetPro: 1.247 ± 0.28
0.453MetGln: 0.453 ± 0.113
1.644MetArg: 1.644 ± 0.297
2.664MetSer: 2.664 ± 0.371
2.664MetThr: 2.664 ± 0.358
1.587MetVal: 1.587 ± 0.307
0.397MetTrp: 0.397 ± 0.129
0.453MetTyr: 0.453 ± 0.151
0.0MetXaa: 0.0 ± 0.0
Asn
3.514AsnAla: 3.514 ± 0.427
0.227AsnCys: 0.227 ± 0.122
2.04AsnAsp: 2.04 ± 0.311
1.814AsnGlu: 1.814 ± 0.355
0.794AsnPhe: 0.794 ± 0.243
4.534AsnGly: 4.534 ± 0.579
0.68AsnHis: 0.68 ± 0.184
1.587AsnIle: 1.587 ± 0.501
1.19AsnLys: 1.19 ± 0.262
2.381AsnLeu: 2.381 ± 0.343
0.737AsnMet: 0.737 ± 0.175
1.757AsnAsn: 1.757 ± 0.369
2.324AsnPro: 2.324 ± 0.366
1.304AsnGln: 1.304 ± 0.324
1.984AsnArg: 1.984 ± 0.364
1.644AsnSer: 1.644 ± 0.358
2.211AsnThr: 2.211 ± 0.356
2.097AsnVal: 2.097 ± 0.352
0.623AsnTrp: 0.623 ± 0.184
0.794AsnTyr: 0.794 ± 0.187
0.0AsnXaa: 0.0 ± 0.0
Pro
5.158ProAla: 5.158 ± 0.65
0.737ProCys: 0.737 ± 0.23
4.251ProAsp: 4.251 ± 0.491
4.251ProGlu: 4.251 ± 0.586
1.7ProPhe: 1.7 ± 0.34
6.461ProGly: 6.461 ± 0.664
1.87ProHis: 1.87 ± 0.323
1.757ProIle: 1.757 ± 0.345
2.551ProLys: 2.551 ± 0.532
4.648ProLeu: 4.648 ± 0.588
1.36ProMet: 1.36 ± 0.293
2.097ProAsn: 2.097 ± 0.286
4.024ProPro: 4.024 ± 0.508
2.381ProGln: 2.381 ± 0.371
3.628ProArg: 3.628 ± 0.642
3.174ProSer: 3.174 ± 0.494
3.628ProThr: 3.628 ± 0.486
4.421ProVal: 4.421 ± 0.459
1.134ProTrp: 1.134 ± 0.22
1.19ProTyr: 1.19 ± 0.261
0.0ProXaa: 0.0 ± 0.0
Gln
4.138GlnAla: 4.138 ± 0.552
0.567GlnCys: 0.567 ± 0.235
1.247GlnAsp: 1.247 ± 0.223
1.87GlnGlu: 1.87 ± 0.384
1.247GlnPhe: 1.247 ± 0.283
2.721GlnGly: 2.721 ± 0.446
0.794GlnHis: 0.794 ± 0.183
1.927GlnIle: 1.927 ± 0.327
1.19GlnLys: 1.19 ± 0.207
3.061GlnLeu: 3.061 ± 0.411
0.737GlnMet: 0.737 ± 0.219
0.794GlnAsn: 0.794 ± 0.271
3.174GlnPro: 3.174 ± 0.414
1.53GlnGln: 1.53 ± 0.32
2.267GlnArg: 2.267 ± 0.349
1.984GlnSer: 1.984 ± 0.294
1.474GlnThr: 1.474 ± 0.237
2.551GlnVal: 2.551 ± 0.337
0.794GlnTrp: 0.794 ± 0.182
0.964GlnTyr: 0.964 ± 0.281
0.0GlnXaa: 0.0 ± 0.0
Arg
6.972ArgAla: 6.972 ± 0.717
1.134ArgCys: 1.134 ± 0.313
4.251ArgAsp: 4.251 ± 0.501
4.704ArgGlu: 4.704 ± 0.639
1.87ArgPhe: 1.87 ± 0.409
4.194ArgGly: 4.194 ± 0.529
1.19ArgHis: 1.19 ± 0.259
3.741ArgIle: 3.741 ± 0.56
2.324ArgLys: 2.324 ± 0.421
5.158ArgLeu: 5.158 ± 0.636
2.437ArgMet: 2.437 ± 0.395
2.324ArgAsn: 2.324 ± 0.41
3.457ArgPro: 3.457 ± 0.445
1.927ArgGln: 1.927 ± 0.433
5.668ArgArg: 5.668 ± 0.817
3.911ArgSer: 3.911 ± 0.429
4.194ArgThr: 4.194 ± 0.501
5.385ArgVal: 5.385 ± 0.709
1.814ArgTrp: 1.814 ± 0.355
2.097ArgTyr: 2.097 ± 0.294
0.0ArgXaa: 0.0 ± 0.0
Ser
6.688SerAla: 6.688 ± 1.06
0.453SerCys: 0.453 ± 0.188
4.534SerAsp: 4.534 ± 0.517
2.607SerGlu: 2.607 ± 0.415
1.644SerPhe: 1.644 ± 0.234
6.802SerGly: 6.802 ± 0.906
1.134SerHis: 1.134 ± 0.217
3.004SerIle: 3.004 ± 0.423
2.551SerLys: 2.551 ± 0.421
3.401SerLeu: 3.401 ± 0.433
1.19SerMet: 1.19 ± 0.238
2.494SerAsn: 2.494 ± 0.483
3.684SerPro: 3.684 ± 0.397
1.474SerGln: 1.474 ± 0.283
3.344SerArg: 3.344 ± 0.388
3.741SerSer: 3.741 ± 0.688
3.344SerThr: 3.344 ± 0.518
4.988SerVal: 4.988 ± 0.673
1.19SerTrp: 1.19 ± 0.248
1.247SerTyr: 1.247 ± 0.208
0.0SerXaa: 0.0 ± 0.0
Thr
6.802ThrAla: 6.802 ± 0.658
0.567ThrCys: 0.567 ± 0.173
4.024ThrAsp: 4.024 ± 0.54
3.854ThrGlu: 3.854 ± 0.394
1.757ThrPhe: 1.757 ± 0.3
6.461ThrGly: 6.461 ± 0.594
2.381ThrHis: 2.381 ± 0.34
4.024ThrIle: 4.024 ± 0.486
2.097ThrLys: 2.097 ± 0.302
4.081ThrLeu: 4.081 ± 0.537
1.247ThrMet: 1.247 ± 0.272
2.04ThrAsn: 2.04 ± 0.408
5.044ThrPro: 5.044 ± 0.539
2.324ThrGln: 2.324 ± 0.372
4.421ThrArg: 4.421 ± 0.569
3.854ThrSer: 3.854 ± 0.51
4.591ThrThr: 4.591 ± 0.621
6.405ThrVal: 6.405 ± 0.556
0.964ThrTrp: 0.964 ± 0.273
1.814ThrTyr: 1.814 ± 0.333
0.0ThrXaa: 0.0 ± 0.0
Val
7.708ValAla: 7.708 ± 0.83
1.077ValCys: 1.077 ± 0.293
4.988ValAsp: 4.988 ± 0.509
4.534ValGlu: 4.534 ± 0.59
2.267ValPhe: 2.267 ± 0.357
5.781ValGly: 5.781 ± 0.646
1.417ValHis: 1.417 ± 0.294
2.777ValIle: 2.777 ± 0.393
2.664ValLys: 2.664 ± 0.365
4.931ValLeu: 4.931 ± 0.537
1.304ValMet: 1.304 ± 0.261
2.551ValAsn: 2.551 ± 0.398
3.798ValPro: 3.798 ± 0.385
2.664ValGln: 2.664 ± 0.324
4.308ValArg: 4.308 ± 0.523
5.498ValSer: 5.498 ± 0.622
5.441ValThr: 5.441 ± 0.565
6.235ValVal: 6.235 ± 0.733
1.814ValTrp: 1.814 ± 0.335
1.36ValTyr: 1.36 ± 0.299
0.0ValXaa: 0.0 ± 0.0
Trp
1.984TrpAla: 1.984 ± 0.304
0.227TrpCys: 0.227 ± 0.115
1.417TrpAsp: 1.417 ± 0.248
1.247TrpGlu: 1.247 ± 0.364
0.794TrpPhe: 0.794 ± 0.208
1.02TrpGly: 1.02 ± 0.228
0.51TrpHis: 0.51 ± 0.174
1.53TrpIle: 1.53 ± 0.247
0.85TrpLys: 0.85 ± 0.186
1.644TrpLeu: 1.644 ± 0.365
0.85TrpMet: 0.85 ± 0.238
0.623TrpAsn: 0.623 ± 0.209
0.794TrpPro: 0.794 ± 0.247
0.85TrpGln: 0.85 ± 0.223
2.267TrpArg: 2.267 ± 0.418
1.644TrpSer: 1.644 ± 0.381
1.587TrpThr: 1.587 ± 0.317
1.757TrpVal: 1.757 ± 0.433
1.077TrpTrp: 1.077 ± 0.221
0.397TrpTyr: 0.397 ± 0.151
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.607TyrAla: 2.607 ± 0.388
0.283TyrCys: 0.283 ± 0.126
1.87TyrAsp: 1.87 ± 0.42
1.814TyrGlu: 1.814 ± 0.335
0.737TyrPhe: 0.737 ± 0.221
2.04TyrGly: 2.04 ± 0.362
0.34TyrHis: 0.34 ± 0.109
1.36TyrIle: 1.36 ± 0.282
0.68TyrLys: 0.68 ± 0.187
1.927TyrLeu: 1.927 ± 0.266
0.113TyrMet: 0.113 ± 0.076
0.737TyrAsn: 0.737 ± 0.206
1.36TyrPro: 1.36 ± 0.265
0.737TyrGln: 0.737 ± 0.233
1.757TyrArg: 1.757 ± 0.33
0.794TyrSer: 0.794 ± 0.209
1.814TyrThr: 1.814 ± 0.346
2.154TyrVal: 2.154 ± 0.326
0.51TyrTrp: 0.51 ± 0.154
0.51TyrTyr: 0.51 ± 0.151
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 102 proteins (17644 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski