Morganella phage IME1369_02

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JP24|A0A1W6JP24_9CAUD Uncharacterized protein OS=Morganella phage IME1369_02 OX=1965488 PE=4 SV=1
MM1 pKa = 8.02SEE3 pKa = 3.87TTYY6 pKa = 11.28KK7 pKa = 10.16AVSWGEE13 pKa = 3.92VKK15 pKa = 10.75ADD17 pKa = 3.59VYY19 pKa = 11.31SGEE22 pKa = 4.21SCNEE26 pKa = 3.36HH27 pKa = 6.57RR28 pKa = 11.84PFIEE32 pKa = 4.12MLCDD36 pKa = 4.26GDD38 pKa = 4.09MEE40 pKa = 4.84SCIEE44 pKa = 4.23DD45 pKa = 3.32EE46 pKa = 4.58VTFRR50 pKa = 11.84ASRR53 pKa = 11.84WPVGTKK59 pKa = 8.52ITVEE63 pKa = 4.48VPCCPVCSLDD73 pKa = 5.72AEE75 pKa = 4.51YY76 pKa = 10.65QDD78 pKa = 3.22KK79 pKa = 11.01AGRR82 pKa = 11.84CEE84 pKa = 5.57CGFDD88 pKa = 3.09WKK90 pKa = 10.67EE91 pKa = 3.6WAEE94 pKa = 4.11NKK96 pKa = 10.37YY97 pKa = 10.88SS98 pKa = 3.35

Molecular weight: 11.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JP45|A0A1W6JP45_9CAUD Uncharacterized protein OS=Morganella phage IME1369_02 OX=1965488 PE=4 SV=1
MM1 pKa = 6.54QAGRR5 pKa = 11.84LRR7 pKa = 11.84HH8 pKa = 5.62RR9 pKa = 11.84VTFQRR14 pKa = 11.84AEE16 pKa = 4.1RR17 pKa = 11.84VTLPSGQRR25 pKa = 11.84EE26 pKa = 4.33NQWLPVAVTWAEE38 pKa = 4.0VKK40 pKa = 9.99PVSGRR45 pKa = 11.84EE46 pKa = 4.07LITAGAEE53 pKa = 3.8MSEE56 pKa = 3.77ITVRR60 pKa = 11.84VWMRR64 pKa = 11.84YY65 pKa = 9.46RR66 pKa = 11.84PDD68 pKa = 2.78IHH70 pKa = 6.36PACRR74 pKa = 11.84MVYY77 pKa = 9.85RR78 pKa = 11.84GQVYY82 pKa = 10.02DD83 pKa = 3.75IQAVIPDD90 pKa = 3.64VKK92 pKa = 10.15FTRR95 pKa = 11.84LEE97 pKa = 4.09LLCKK101 pKa = 10.37QGVKK105 pKa = 10.59DD106 pKa = 3.73GG107 pKa = 3.93

Molecular weight: 12.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

11941

46

1183

199.0

22.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.86 ± 0.489

1.323 ± 0.162

5.845 ± 0.288

6.733 ± 0.234

3.459 ± 0.186

7.21 ± 0.345

2.152 ± 0.225

5.703 ± 0.212

5.728 ± 0.268

7.83 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.856 ± 0.155

4.288 ± 0.207

4.179 ± 0.234

4.087 ± 0.263

5.896 ± 0.261

6.666 ± 0.298

5.762 ± 0.269

6.373 ± 0.275

1.75 ± 0.115

3.3 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski