Bacteroides sp. OF04-15BH

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; unclassified Bacteroides

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3052 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A416GAY5|A0A416GAY5_9BACE 50S ribosomal protein L11 OS=Bacteroides sp. OF04-15BH OX=2292281 GN=rplK PE=3 SV=1
MM1 pKa = 7.63KK2 pKa = 9.34KK3 pKa = 9.02TIVVYY8 pKa = 10.52GSSTGTCEE16 pKa = 3.73EE17 pKa = 4.33LAGKK21 pKa = 9.64IATALGLDD29 pKa = 3.88DD30 pKa = 4.0SAVINVSEE38 pKa = 4.81LTPDD42 pKa = 3.82MISEE46 pKa = 4.1NEE48 pKa = 3.81ALILGSATWGCGEE61 pKa = 5.15LQDD64 pKa = 4.94DD65 pKa = 4.48WYY67 pKa = 10.79DD68 pKa = 3.59GVKK71 pKa = 10.23VLQSADD77 pKa = 3.69LNGKK81 pKa = 8.84IVALFGCGDD90 pKa = 3.7SEE92 pKa = 5.35SYY94 pKa = 11.7GDD96 pKa = 4.07TFCDD100 pKa = 4.82AIGLLYY106 pKa = 10.44QEE108 pKa = 5.29IKK110 pKa = 10.96DD111 pKa = 4.14KK112 pKa = 11.43GCTLAGSGISTDD124 pKa = 4.14GYY126 pKa = 10.79LHH128 pKa = 6.7TDD130 pKa = 3.23SVALDD135 pKa = 3.45AEE137 pKa = 5.15GNFLGLALDD146 pKa = 4.25EE147 pKa = 4.57VNQPEE152 pKa = 4.47LTDD155 pKa = 3.42SRR157 pKa = 11.84IAAWVEE163 pKa = 4.02QIAPALQQ170 pKa = 3.53

Molecular weight: 17.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A416G344|A0A416G344_9BACE DNA-binding protein (Fragment) OS=Bacteroides sp. OF04-15BH OX=2292281 GN=DXA74_15985 PE=4 SV=1
MM1 pKa = 6.97QKK3 pKa = 10.28KK4 pKa = 10.11GEE6 pKa = 4.16KK7 pKa = 9.37TSLEE11 pKa = 4.14NLVPICPALSRR22 pKa = 11.84FVHH25 pKa = 5.67QPPILQHH32 pKa = 5.72YY33 pKa = 8.45KK34 pKa = 10.75SPLLCSGVTNRR45 pKa = 11.84RR46 pKa = 11.84GRR48 pKa = 11.84RR49 pKa = 11.84CKK51 pKa = 9.97TGLVNGWAYY60 pKa = 10.88RR61 pKa = 11.84STLTPIRR68 pKa = 11.84PP69 pKa = 3.51

Molecular weight: 7.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3052

0

3052

1099428

23

2524

360.2

40.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.255 ± 0.035

1.372 ± 0.017

5.41 ± 0.03

6.767 ± 0.044

4.514 ± 0.032

6.676 ± 0.042

1.944 ± 0.021

6.387 ± 0.04

6.411 ± 0.038

9.461 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.761 ± 0.017

4.837 ± 0.036

3.756 ± 0.025

3.96 ± 0.026

4.959 ± 0.037

5.981 ± 0.031

5.474 ± 0.031

6.473 ± 0.034

1.277 ± 0.019

4.323 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski