Alkalibacter saccharofermentans DSM 14828

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Alkalibacter; Alkalibacter saccharofermentans

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2181 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M4WK38|A0A1M4WK38_9FIRM MinD superfamily P-loop ATPase contains an inserted ferredoxin domain OS=Alkalibacter saccharofermentans DSM 14828 OX=1120975 GN=SAMN02746064_01266 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.62MKK4 pKa = 10.51KK5 pKa = 10.16FLVFLLAALLILSLAACGGNGDD27 pKa = 4.17TNGSQVTDD35 pKa = 3.38NGEE38 pKa = 3.98GSSEE42 pKa = 4.2SEE44 pKa = 3.57EE45 pKa = 5.22DD46 pKa = 3.1ITFEE50 pKa = 5.46DD51 pKa = 3.7SLKK54 pKa = 11.24AEE56 pKa = 5.23GIYY59 pKa = 10.41EE60 pKa = 3.96NYY62 pKa = 8.82YY63 pKa = 10.72FEE65 pKa = 4.92MEE67 pKa = 4.35VNVNGEE73 pKa = 4.47DD74 pKa = 4.08FPTSQLWFLGGDD86 pKa = 3.42MKK88 pKa = 11.26FEE90 pKa = 4.31NQDD93 pKa = 2.9QAIFIKK99 pKa = 10.51PSEE102 pKa = 4.26EE103 pKa = 3.6QMGIYY108 pKa = 9.39TKK110 pKa = 9.45EE111 pKa = 4.08TNQLVLMPISEE122 pKa = 4.45NEE124 pKa = 3.81DD125 pKa = 3.82FYY127 pKa = 11.28TPFIAADD134 pKa = 4.03EE135 pKa = 4.34IEE137 pKa = 4.24EE138 pKa = 4.02EE139 pKa = 4.6VYY141 pKa = 10.88EE142 pKa = 4.76GILYY146 pKa = 10.19KK147 pKa = 10.84GSEE150 pKa = 4.22TLDD153 pKa = 3.43GKK155 pKa = 9.05TVDD158 pKa = 3.44VFEE161 pKa = 4.54YY162 pKa = 11.17DD163 pKa = 4.29AMDD166 pKa = 3.87VYY168 pKa = 11.42AKK170 pKa = 10.57YY171 pKa = 10.71YY172 pKa = 8.63IWRR175 pKa = 11.84DD176 pKa = 3.02TGIIVKK182 pKa = 9.06MIVHH186 pKa = 5.96SDD188 pKa = 3.45EE189 pKa = 4.65YY190 pKa = 10.34EE191 pKa = 3.82SEE193 pKa = 4.63YY194 pKa = 10.8YY195 pKa = 9.39FRR197 pKa = 11.84NMRR200 pKa = 11.84VNEE203 pKa = 4.0LTADD207 pKa = 3.76DD208 pKa = 4.24LAYY211 pKa = 10.13PEE213 pKa = 5.08DD214 pKa = 4.42AEE216 pKa = 4.26IMDD219 pKa = 3.51MSEE222 pKa = 4.87FLVQQ226 pKa = 3.89

Molecular weight: 25.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M4T900|A0A1M4T900_9FIRM EamA-like transporter family protein OS=Alkalibacter saccharofermentans DSM 14828 OX=1120975 GN=SAMN02746064_00419 PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 8.52MTYY5 pKa = 8.4QPKK8 pKa = 8.68VRR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.44GFRR19 pKa = 11.84QRR21 pKa = 11.84MKK23 pKa = 9.4SASGRR28 pKa = 11.84KK29 pKa = 8.73VLSNRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.97GRR39 pKa = 11.84KK40 pKa = 8.84RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2181

0

2181

692899

40

2944

317.7

35.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.627 ± 0.047

1.029 ± 0.02

5.938 ± 0.047

7.559 ± 0.055

4.271 ± 0.04

7.279 ± 0.049

1.657 ± 0.022

8.735 ± 0.047

7.862 ± 0.052

9.124 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.052 ± 0.028

4.723 ± 0.038

3.216 ± 0.029

2.514 ± 0.023

3.953 ± 0.031

6.188 ± 0.037

4.845 ± 0.037

6.944 ± 0.041

0.736 ± 0.017

3.747 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski