Estrella lausannensis

Taxonomy: cellular organisms; Bacteria; PVC group; Chlamydiae; Chlamydiia; Parachlamydiales; Criblamydiaceae; Estrella

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2216 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H5DN22|A0A0H5DN22_9BACT Putative caspase OS=Estrella lausannensis OX=483423 GN=ELAC_0250 PE=4 SV=1
MM1 pKa = 7.06NQFHH5 pKa = 7.42LIKK8 pKa = 10.03TFFLTTLIPLNLMSAPAFIEE28 pKa = 4.48MIEE31 pKa = 4.51GPQASCACQPPQQGPEE47 pKa = 4.29GPPGEE52 pKa = 4.63AGNPFTTLGSNYY64 pKa = 10.15YY65 pKa = 9.8IDD67 pKa = 5.17GDD69 pKa = 3.9TDD71 pKa = 3.57IDD73 pKa = 3.99PGEE76 pKa = 4.5QIPIDD81 pKa = 3.37IDD83 pKa = 3.59GSNVNVVRR91 pKa = 11.84LNDD94 pKa = 3.4FQFQVEE100 pKa = 4.07EE101 pKa = 4.13AGNYY105 pKa = 10.15FIMLGCTSNDD115 pKa = 3.08QLEE118 pKa = 4.46QGNSATLGLFVNGSFVTGSEE138 pKa = 3.92LSLVRR143 pKa = 11.84NSKK146 pKa = 10.48LYY148 pKa = 10.02NLSLIVPLQVGDD160 pKa = 3.88ILDD163 pKa = 3.92LRR165 pKa = 11.84STAEE169 pKa = 4.02EE170 pKa = 4.1SWVIGSDD177 pKa = 4.04YY178 pKa = 11.17YY179 pKa = 11.56SGLLWGTYY187 pKa = 7.32VTATLTLFRR196 pKa = 11.84IGPAPVNN203 pKa = 3.57

Molecular weight: 22.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H5DNM6|A0A0H5DNM6_9BACT Leucine rich repeat protein OS=Estrella lausannensis OX=483423 GN=ELAC_0024 PE=4 SV=1
MM1 pKa = 7.8PEE3 pKa = 4.11KK4 pKa = 10.45KK5 pKa = 10.02KK6 pKa = 9.69KK7 pKa = 9.11TSAKK11 pKa = 8.32STKK14 pKa = 10.05SQGKK18 pKa = 7.41STTVVASVKK27 pKa = 10.3KK28 pKa = 9.84KK29 pKa = 10.86AGVKK33 pKa = 9.32SAKK36 pKa = 8.5QTTAAGAAKK45 pKa = 10.23AKK47 pKa = 10.22RR48 pKa = 11.84AGAASKK54 pKa = 10.7PPVKK58 pKa = 10.52AKK60 pKa = 10.11AAPKK64 pKa = 9.82RR65 pKa = 11.84GQAHH69 pKa = 6.92KK70 pKa = 8.73PTAKK74 pKa = 10.29AASTTRR80 pKa = 11.84SKK82 pKa = 11.05AVKK85 pKa = 9.78KK86 pKa = 10.25GSSSAASRR94 pKa = 11.84TAPSRR99 pKa = 11.84QAAKK103 pKa = 10.08KK104 pKa = 9.82SSPAARR110 pKa = 11.84AKK112 pKa = 10.44APKK115 pKa = 9.65KK116 pKa = 10.04AAPKK120 pKa = 9.41SAKK123 pKa = 9.36QRR125 pKa = 11.84APVAKK130 pKa = 9.56KK131 pKa = 9.92AAVAKK136 pKa = 10.38GSAKK140 pKa = 10.19RR141 pKa = 11.84SLPAKK146 pKa = 10.1KK147 pKa = 9.96KK148 pKa = 10.28VATQGAKK155 pKa = 9.84KK156 pKa = 9.73RR157 pKa = 11.84APVAKK162 pKa = 9.48KK163 pKa = 8.62GTKK166 pKa = 10.13AGVKK170 pKa = 9.92SKK172 pKa = 10.32SARR175 pKa = 3.44

Molecular weight: 17.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2216

0

2216

808995

34

4173

365.1

40.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.041 ± 0.048

1.095 ± 0.021

4.948 ± 0.032

7.483 ± 0.062

4.831 ± 0.039

6.619 ± 0.048

2.154 ± 0.024

6.546 ± 0.048

7.014 ± 0.059

10.913 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.37 ± 0.023

3.467 ± 0.031

4.419 ± 0.036

3.631 ± 0.032

4.911 ± 0.037

6.888 ± 0.045

4.809 ± 0.031

5.917 ± 0.038

1.057 ± 0.018

2.885 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski