Amino acid dipepetide frequency for Arthrobacter phage Adat

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.201AlaAla: 7.201 ± 0.846
0.36AlaCys: 0.36 ± 0.152
4.321AlaAsp: 4.321 ± 0.565
5.329AlaGlu: 5.329 ± 0.606
2.376AlaPhe: 2.376 ± 0.307
6.481AlaGly: 6.481 ± 0.869
2.16AlaHis: 2.16 ± 0.371
4.393AlaIle: 4.393 ± 0.676
5.833AlaLys: 5.833 ± 0.968
6.913AlaLeu: 6.913 ± 0.675
2.809AlaMet: 2.809 ± 0.436
5.545AlaAsn: 5.545 ± 0.75
3.817AlaPro: 3.817 ± 0.641
4.105AlaGln: 4.105 ± 0.771
3.673AlaArg: 3.673 ± 0.599
4.033AlaSer: 4.033 ± 0.544
5.041AlaThr: 5.041 ± 0.615
5.185AlaVal: 5.185 ± 0.557
1.008AlaTrp: 1.008 ± 0.283
3.241AlaTyr: 3.241 ± 0.474
0.0AlaXaa: 0.0 ± 0.0
Cys
0.432CysAla: 0.432 ± 0.205
0.144CysCys: 0.144 ± 0.105
0.288CysAsp: 0.288 ± 0.14
0.504CysGlu: 0.504 ± 0.193
0.0CysPhe: 0.0 ± 0.0
0.432CysGly: 0.432 ± 0.169
0.144CysHis: 0.144 ± 0.086
0.144CysIle: 0.144 ± 0.093
0.432CysLys: 0.432 ± 0.181
0.432CysLeu: 0.432 ± 0.204
0.36CysMet: 0.36 ± 0.153
0.216CysAsn: 0.216 ± 0.167
0.216CysPro: 0.216 ± 0.136
0.144CysGln: 0.144 ± 0.108
0.576CysArg: 0.576 ± 0.206
0.504CysSer: 0.504 ± 0.237
0.432CysThr: 0.432 ± 0.186
0.432CysVal: 0.432 ± 0.181
0.072CysTrp: 0.072 ± 0.067
0.072CysTyr: 0.072 ± 0.077
0.0CysXaa: 0.0 ± 0.0
Asp
4.537AspAla: 4.537 ± 0.542
0.0AspCys: 0.0 ± 0.0
3.313AspAsp: 3.313 ± 0.547
4.321AspGlu: 4.321 ± 0.634
3.169AspPhe: 3.169 ± 0.515
5.113AspGly: 5.113 ± 0.622
0.72AspHis: 0.72 ± 0.205
4.321AspIle: 4.321 ± 0.503
4.033AspLys: 4.033 ± 0.552
4.969AspLeu: 4.969 ± 0.708
2.304AspMet: 2.304 ± 0.403
3.313AspAsn: 3.313 ± 0.506
3.529AspPro: 3.529 ± 0.561
2.232AspGln: 2.232 ± 0.396
2.737AspArg: 2.737 ± 0.458
3.457AspSer: 3.457 ± 0.61
2.809AspThr: 2.809 ± 0.447
4.537AspVal: 4.537 ± 0.575
0.936AspTrp: 0.936 ± 0.227
1.728AspTyr: 1.728 ± 0.273
0.0AspXaa: 0.0 ± 0.0
Glu
6.553GluAla: 6.553 ± 0.865
0.648GluCys: 0.648 ± 0.282
4.177GluAsp: 4.177 ± 0.542
5.977GluGlu: 5.977 ± 1.269
3.817GluPhe: 3.817 ± 0.371
3.385GluGly: 3.385 ± 0.49
1.08GluHis: 1.08 ± 0.306
4.393GluIle: 4.393 ± 0.662
4.897GluLys: 4.897 ± 0.53
4.681GluLeu: 4.681 ± 0.739
2.521GluMet: 2.521 ± 0.468
4.681GluAsn: 4.681 ± 0.621
2.232GluPro: 2.232 ± 0.601
3.241GluGln: 3.241 ± 0.513
2.809GluArg: 2.809 ± 0.452
4.681GluSer: 4.681 ± 0.791
4.249GluThr: 4.249 ± 0.606
4.105GluVal: 4.105 ± 0.515
0.864GluTrp: 0.864 ± 0.231
2.376GluTyr: 2.376 ± 0.536
0.0GluXaa: 0.0 ± 0.0
Phe
2.521PheAla: 2.521 ± 0.48
0.288PheCys: 0.288 ± 0.145
3.601PheAsp: 3.601 ± 0.586
2.953PheGlu: 2.953 ± 0.379
1.728PhePhe: 1.728 ± 0.278
2.737PheGly: 2.737 ± 0.467
1.584PheHis: 1.584 ± 0.339
2.376PheIle: 2.376 ± 0.388
2.232PheLys: 2.232 ± 0.428
2.665PheLeu: 2.665 ± 0.452
0.864PheMet: 0.864 ± 0.228
2.521PheAsn: 2.521 ± 0.448
1.512PhePro: 1.512 ± 0.338
1.152PheGln: 1.152 ± 0.267
1.8PheArg: 1.8 ± 0.286
2.809PheSer: 2.809 ± 0.463
2.304PheThr: 2.304 ± 0.395
2.449PheVal: 2.449 ± 0.411
0.36PheTrp: 0.36 ± 0.159
1.08PheTyr: 1.08 ± 0.251
0.0PheXaa: 0.0 ± 0.0
Gly
4.753GlyAla: 4.753 ± 0.662
0.144GlyCys: 0.144 ± 0.125
3.889GlyAsp: 3.889 ± 0.777
3.601GlyGlu: 3.601 ± 0.427
3.889GlyPhe: 3.889 ± 0.464
6.769GlyGly: 6.769 ± 0.684
0.792GlyHis: 0.792 ± 0.241
4.537GlyIle: 4.537 ± 0.55
5.473GlyLys: 5.473 ± 0.861
5.113GlyLeu: 5.113 ± 0.538
2.449GlyMet: 2.449 ± 0.468
3.673GlyAsn: 3.673 ± 0.451
2.16GlyPro: 2.16 ± 0.443
2.449GlyGln: 2.449 ± 0.461
3.241GlyArg: 3.241 ± 0.441
5.977GlySer: 5.977 ± 0.796
5.185GlyThr: 5.185 ± 0.7
4.825GlyVal: 4.825 ± 0.522
1.512GlyTrp: 1.512 ± 0.27
2.304GlyTyr: 2.304 ± 0.541
0.0GlyXaa: 0.0 ± 0.0
His
1.08HisAla: 1.08 ± 0.24
0.0HisCys: 0.0 ± 0.0
1.224HisAsp: 1.224 ± 0.251
1.008HisGlu: 1.008 ± 0.229
0.792HisPhe: 0.792 ± 0.202
1.512HisGly: 1.512 ± 0.301
0.648HisHis: 0.648 ± 0.188
1.008HisIle: 1.008 ± 0.229
1.944HisLys: 1.944 ± 0.345
1.08HisLeu: 1.08 ± 0.279
0.792HisMet: 0.792 ± 0.241
1.224HisAsn: 1.224 ± 0.261
0.72HisPro: 0.72 ± 0.242
0.648HisGln: 0.648 ± 0.173
1.224HisArg: 1.224 ± 0.336
0.72HisSer: 0.72 ± 0.216
0.864HisThr: 0.864 ± 0.271
1.368HisVal: 1.368 ± 0.329
0.36HisTrp: 0.36 ± 0.185
0.36HisTyr: 0.36 ± 0.17
0.0HisXaa: 0.0 ± 0.0
Ile
5.617IleAla: 5.617 ± 0.741
0.648IleCys: 0.648 ± 0.22
3.241IleAsp: 3.241 ± 0.502
4.177IleGlu: 4.177 ± 0.561
2.088IlePhe: 2.088 ± 0.33
3.241IleGly: 3.241 ± 0.478
1.152IleHis: 1.152 ± 0.266
2.376IleIle: 2.376 ± 0.399
3.025IleLys: 3.025 ± 0.467
3.961IleLeu: 3.961 ± 0.496
1.224IleMet: 1.224 ± 0.265
3.241IleAsn: 3.241 ± 0.413
2.232IlePro: 2.232 ± 0.337
3.313IleGln: 3.313 ± 0.462
2.953IleArg: 2.953 ± 0.488
3.673IleSer: 3.673 ± 0.448
3.313IleThr: 3.313 ± 0.507
2.521IleVal: 2.521 ± 0.434
0.648IleTrp: 0.648 ± 0.212
1.296IleTyr: 1.296 ± 0.326
0.0IleXaa: 0.0 ± 0.0
Lys
6.121LysAla: 6.121 ± 0.825
0.36LysCys: 0.36 ± 0.159
3.817LysAsp: 3.817 ± 0.517
5.617LysGlu: 5.617 ± 0.581
2.304LysPhe: 2.304 ± 0.378
2.953LysGly: 2.953 ± 0.597
0.648LysHis: 0.648 ± 0.2
3.097LysIle: 3.097 ± 0.364
4.033LysLys: 4.033 ± 0.535
4.825LysLeu: 4.825 ± 0.751
1.728LysMet: 1.728 ± 0.332
3.385LysAsn: 3.385 ± 0.446
4.033LysPro: 4.033 ± 0.659
2.809LysGln: 2.809 ± 0.392
2.881LysArg: 2.881 ± 0.691
3.601LysSer: 3.601 ± 0.494
4.105LysThr: 4.105 ± 0.635
4.033LysVal: 4.033 ± 0.466
0.936LysTrp: 0.936 ± 0.248
2.521LysTyr: 2.521 ± 0.376
0.0LysXaa: 0.0 ± 0.0
Leu
8.498LeuAla: 8.498 ± 0.677
0.216LeuCys: 0.216 ± 0.127
6.049LeuAsp: 6.049 ± 0.731
5.185LeuGlu: 5.185 ± 0.79
2.232LeuPhe: 2.232 ± 0.41
5.617LeuGly: 5.617 ± 0.68
1.08LeuHis: 1.08 ± 0.222
4.033LeuIle: 4.033 ± 0.531
4.105LeuLys: 4.105 ± 0.53
4.753LeuLeu: 4.753 ± 0.521
1.656LeuMet: 1.656 ± 0.292
3.745LeuAsn: 3.745 ± 0.48
3.745LeuPro: 3.745 ± 0.554
3.313LeuGln: 3.313 ± 0.622
3.097LeuArg: 3.097 ± 0.415
5.185LeuSer: 5.185 ± 0.638
3.673LeuThr: 3.673 ± 0.604
4.033LeuVal: 4.033 ± 0.577
1.152LeuTrp: 1.152 ± 0.21
2.016LeuTyr: 2.016 ± 0.395
0.0LeuXaa: 0.0 ± 0.0
Met
2.953MetAla: 2.953 ± 0.408
0.072MetCys: 0.072 ± 0.07
1.008MetAsp: 1.008 ± 0.228
1.944MetGlu: 1.944 ± 0.392
0.864MetPhe: 0.864 ± 0.213
2.376MetGly: 2.376 ± 0.39
0.72MetHis: 0.72 ± 0.188
1.08MetIle: 1.08 ± 0.266
2.665MetLys: 2.665 ± 0.388
2.088MetLeu: 2.088 ± 0.421
0.504MetMet: 0.504 ± 0.207
1.08MetAsn: 1.08 ± 0.269
1.512MetPro: 1.512 ± 0.294
0.936MetGln: 0.936 ± 0.229
1.44MetArg: 1.44 ± 0.244
2.088MetSer: 2.088 ± 0.389
1.656MetThr: 1.656 ± 0.252
2.232MetVal: 2.232 ± 0.36
0.432MetTrp: 0.432 ± 0.159
0.936MetTyr: 0.936 ± 0.212
0.0MetXaa: 0.0 ± 0.0
Asn
4.681AsnAla: 4.681 ± 0.497
0.576AsnCys: 0.576 ± 0.194
3.817AsnAsp: 3.817 ± 0.447
4.249AsnGlu: 4.249 ± 0.599
2.016AsnPhe: 2.016 ± 0.336
4.537AsnGly: 4.537 ± 0.658
0.72AsnHis: 0.72 ± 0.191
2.809AsnIle: 2.809 ± 0.556
3.241AsnLys: 3.241 ± 0.55
3.961AsnLeu: 3.961 ± 0.388
1.728AsnMet: 1.728 ± 0.332
2.809AsnAsn: 2.809 ± 0.564
3.313AsnPro: 3.313 ± 0.496
1.944AsnGln: 1.944 ± 0.39
2.593AsnArg: 2.593 ± 0.415
2.881AsnSer: 2.881 ± 0.543
2.953AsnThr: 2.953 ± 0.456
3.025AsnVal: 3.025 ± 0.438
0.504AsnTrp: 0.504 ± 0.229
1.8AsnTyr: 1.8 ± 0.397
0.0AsnXaa: 0.0 ± 0.0
Pro
3.169ProAla: 3.169 ± 0.557
0.072ProCys: 0.072 ± 0.074
3.241ProAsp: 3.241 ± 0.497
4.753ProGlu: 4.753 ± 0.711
2.16ProPhe: 2.16 ± 0.295
2.593ProGly: 2.593 ± 0.431
0.648ProHis: 0.648 ± 0.258
1.944ProIle: 1.944 ± 0.331
3.097ProLys: 3.097 ± 0.429
2.881ProLeu: 2.881 ± 0.485
1.08ProMet: 1.08 ± 0.324
2.449ProAsn: 2.449 ± 0.453
2.088ProPro: 2.088 ± 0.766
2.304ProGln: 2.304 ± 0.33
1.656ProArg: 1.656 ± 0.285
3.169ProSer: 3.169 ± 0.439
3.457ProThr: 3.457 ± 0.52
3.889ProVal: 3.889 ± 0.559
0.648ProTrp: 0.648 ± 0.2
0.936ProTyr: 0.936 ± 0.22
0.0ProXaa: 0.0 ± 0.0
Gln
3.889GlnAla: 3.889 ± 0.613
0.144GlnCys: 0.144 ± 0.098
2.376GlnAsp: 2.376 ± 0.449
2.953GlnGlu: 2.953 ± 0.51
1.728GlnPhe: 1.728 ± 0.322
2.809GlnGly: 2.809 ± 0.375
0.648GlnHis: 0.648 ± 0.228
2.16GlnIle: 2.16 ± 0.464
3.241GlnLys: 3.241 ± 0.431
3.817GlnLeu: 3.817 ± 0.565
1.152GlnMet: 1.152 ± 0.234
2.449GlnAsn: 2.449 ± 0.55
1.512GlnPro: 1.512 ± 0.356
2.593GlnGln: 2.593 ± 1.024
2.593GlnArg: 2.593 ± 0.498
3.097GlnSer: 3.097 ± 0.517
2.449GlnThr: 2.449 ± 0.443
2.665GlnVal: 2.665 ± 0.54
0.792GlnTrp: 0.792 ± 0.239
1.44GlnTyr: 1.44 ± 0.282
0.0GlnXaa: 0.0 ± 0.0
Arg
3.313ArgAla: 3.313 ± 0.39
0.432ArgCys: 0.432 ± 0.209
2.593ArgAsp: 2.593 ± 0.403
3.313ArgGlu: 3.313 ± 0.566
1.944ArgPhe: 1.944 ± 0.367
2.737ArgGly: 2.737 ± 0.367
1.512ArgHis: 1.512 ± 0.281
3.241ArgIle: 3.241 ± 0.403
2.016ArgLys: 2.016 ± 0.431
3.673ArgLeu: 3.673 ± 0.498
1.44ArgMet: 1.44 ± 0.291
2.521ArgAsn: 2.521 ± 0.473
1.656ArgPro: 1.656 ± 0.299
2.449ArgGln: 2.449 ± 0.436
2.521ArgArg: 2.521 ± 0.506
2.304ArgSer: 2.304 ± 0.422
3.097ArgThr: 3.097 ± 0.429
2.665ArgVal: 2.665 ± 0.502
0.792ArgTrp: 0.792 ± 0.251
2.304ArgTyr: 2.304 ± 0.389
0.0ArgXaa: 0.0 ± 0.0
Ser
4.897SerAla: 4.897 ± 0.683
0.36SerCys: 0.36 ± 0.181
3.745SerAsp: 3.745 ± 0.563
4.753SerGlu: 4.753 ± 0.532
1.512SerPhe: 1.512 ± 0.338
4.969SerGly: 4.969 ± 0.577
0.792SerHis: 0.792 ± 0.255
3.601SerIle: 3.601 ± 0.514
3.457SerLys: 3.457 ± 0.499
5.905SerLeu: 5.905 ± 0.613
1.584SerMet: 1.584 ± 0.36
2.881SerAsn: 2.881 ± 0.667
3.313SerPro: 3.313 ± 0.489
2.953SerGln: 2.953 ± 0.498
2.665SerArg: 2.665 ± 0.397
3.961SerSer: 3.961 ± 0.54
4.033SerThr: 4.033 ± 0.642
4.249SerVal: 4.249 ± 0.508
0.936SerTrp: 0.936 ± 0.326
2.304SerTyr: 2.304 ± 0.55
0.0SerXaa: 0.0 ± 0.0
Thr
5.041ThrAla: 5.041 ± 0.651
0.216ThrCys: 0.216 ± 0.132
3.457ThrAsp: 3.457 ± 0.57
3.169ThrGlu: 3.169 ± 0.453
2.016ThrPhe: 2.016 ± 0.291
5.833ThrGly: 5.833 ± 0.698
1.08ThrHis: 1.08 ± 0.299
3.025ThrIle: 3.025 ± 0.418
3.025ThrLys: 3.025 ± 0.563
4.105ThrLeu: 4.105 ± 0.635
1.728ThrMet: 1.728 ± 0.324
3.313ThrAsn: 3.313 ± 0.578
3.457ThrPro: 3.457 ± 0.502
2.809ThrGln: 2.809 ± 0.504
2.521ThrArg: 2.521 ± 0.479
3.745ThrSer: 3.745 ± 0.558
4.177ThrThr: 4.177 ± 0.663
4.249ThrVal: 4.249 ± 0.818
1.008ThrTrp: 1.008 ± 0.242
2.304ThrTyr: 2.304 ± 0.364
0.0ThrXaa: 0.0 ± 0.0
Val
4.969ValAla: 4.969 ± 0.582
0.576ValCys: 0.576 ± 0.193
4.825ValAsp: 4.825 ± 0.581
4.393ValGlu: 4.393 ± 0.718
2.593ValPhe: 2.593 ± 0.426
5.329ValGly: 5.329 ± 0.715
1.512ValHis: 1.512 ± 0.376
3.241ValIle: 3.241 ± 0.429
3.817ValLys: 3.817 ± 0.43
3.025ValLeu: 3.025 ± 0.481
1.656ValMet: 1.656 ± 0.405
2.376ValAsn: 2.376 ± 0.309
2.953ValPro: 2.953 ± 0.503
2.665ValGln: 2.665 ± 0.356
3.385ValArg: 3.385 ± 0.445
4.609ValSer: 4.609 ± 0.635
4.105ValThr: 4.105 ± 0.55
4.537ValVal: 4.537 ± 0.679
0.72ValTrp: 0.72 ± 0.238
2.304ValTyr: 2.304 ± 0.503
0.0ValXaa: 0.0 ± 0.0
Trp
0.936TrpAla: 0.936 ± 0.249
0.144TrpCys: 0.144 ± 0.101
0.936TrpAsp: 0.936 ± 0.309
1.08TrpGlu: 1.08 ± 0.36
0.432TrpPhe: 0.432 ± 0.187
1.08TrpGly: 1.08 ± 0.293
0.504TrpHis: 0.504 ± 0.215
0.864TrpIle: 0.864 ± 0.245
1.08TrpLys: 1.08 ± 0.302
1.152TrpLeu: 1.152 ± 0.232
0.144TrpMet: 0.144 ± 0.095
1.08TrpAsn: 1.08 ± 0.29
0.576TrpPro: 0.576 ± 0.202
0.792TrpGln: 0.792 ± 0.201
0.648TrpArg: 0.648 ± 0.286
0.72TrpSer: 0.72 ± 0.214
0.864TrpThr: 0.864 ± 0.21
0.72TrpVal: 0.72 ± 0.218
0.216TrpTrp: 0.216 ± 0.116
0.576TrpTyr: 0.576 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.737TyrAla: 2.737 ± 0.388
0.576TyrCys: 0.576 ± 0.205
2.16TyrAsp: 2.16 ± 0.392
2.088TyrGlu: 2.088 ± 0.41
1.872TyrPhe: 1.872 ± 0.436
2.232TyrGly: 2.232 ± 0.478
0.432TyrHis: 0.432 ± 0.17
1.296TyrIle: 1.296 ± 0.295
2.16TyrLys: 2.16 ± 0.298
3.385TyrLeu: 3.385 ± 0.574
0.792TyrMet: 0.792 ± 0.224
1.656TyrAsn: 1.656 ± 0.378
1.656TyrPro: 1.656 ± 0.377
1.656TyrGln: 1.656 ± 0.348
1.512TyrArg: 1.512 ± 0.328
1.656TyrSer: 1.656 ± 0.39
1.512TyrThr: 1.512 ± 0.316
1.872TyrVal: 1.872 ± 0.462
0.648TyrTrp: 0.648 ± 0.195
1.584TyrTyr: 1.584 ± 0.301
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (13887 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski