Mycobacterium phage Journey13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9SFA2|A0A1X9SFA2_9CAUD Minor tail protein OS=Mycobacterium phage Journey13 OX=1983408 GN=SEA_JOURNEY13_26 PE=4 SV=1
MM1 pKa = 7.38YY2 pKa = 10.61RR3 pKa = 11.84LTDD6 pKa = 3.06NHH8 pKa = 6.03GNVWQASDD16 pKa = 4.76IDD18 pKa = 4.3EE19 pKa = 4.57AWDD22 pKa = 3.47AAEE25 pKa = 5.41EE26 pKa = 4.11IATDD30 pKa = 3.44SGIWGGGFDD39 pKa = 4.18IEE41 pKa = 4.47GTEE44 pKa = 4.02VLAYY48 pKa = 9.72DD49 pKa = 4.29AKK51 pKa = 10.94GVAHH55 pKa = 6.15TVGTCVVGG63 pKa = 3.65

Molecular weight: 6.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9SF99|A0A1X9SF99_9CAUD DNA primase OS=Mycobacterium phage Journey13 OX=1983408 GN=SEA_JOURNEY13_53 PE=4 SV=1
MM1 pKa = 7.51SGGTALMPARR11 pKa = 11.84ASIQQTAEE19 pKa = 3.87YY20 pKa = 9.91LGVSTKK26 pKa = 7.26TVRR29 pKa = 11.84RR30 pKa = 11.84YY31 pKa = 9.81IADD34 pKa = 3.74GKK36 pKa = 10.71LKK38 pKa = 10.44AVRR41 pKa = 11.84LGPRR45 pKa = 11.84LIRR48 pKa = 11.84VEE50 pKa = 4.05RR51 pKa = 11.84DD52 pKa = 3.15SVEE55 pKa = 3.78ALMRR59 pKa = 11.84PIGKK63 pKa = 9.51

Molecular weight: 6.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

15017

24

838

185.4

20.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.969 ± 0.36

0.926 ± 0.118

6.439 ± 0.253

7.072 ± 0.323

3.383 ± 0.175

8.337 ± 0.426

1.838 ± 0.165

5.307 ± 0.182

4.362 ± 0.274

8.031 ± 0.37

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.504 ± 0.137

3.336 ± 0.168

5.574 ± 0.222

3.496 ± 0.204

6.599 ± 0.402

5.128 ± 0.224

5.873 ± 0.281

6.932 ± 0.189

1.871 ± 0.155

3.023 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski