Mycobacterium phage Blinn1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VRI1|A0A649VRI1_9CAUD Uncharacterized protein OS=Mycobacterium phage Blinn1 OX=2656562 GN=43 PE=4 SV=1
MM1 pKa = 7.29TPTRR5 pKa = 11.84RR6 pKa = 11.84EE7 pKa = 3.93VMSDD11 pKa = 3.45YY12 pKa = 11.61AWIIDD17 pKa = 3.68KK18 pKa = 11.02DD19 pKa = 3.64HH20 pKa = 6.96LYY22 pKa = 10.97EE23 pKa = 4.37EE24 pKa = 5.26GGICGDD30 pKa = 3.56EE31 pKa = 4.1AGVIGPHH38 pKa = 6.99DD39 pKa = 4.62ANLSEE44 pKa = 5.42GSDD47 pKa = 3.62DD48 pKa = 4.16PKK50 pKa = 11.6AEE52 pKa = 3.95LTRR55 pKa = 11.84NYY57 pKa = 9.6QRR59 pKa = 11.84HH60 pKa = 4.62NQFRR64 pKa = 11.84MYY66 pKa = 11.12DD67 pKa = 3.94DD68 pKa = 5.83DD69 pKa = 6.61DD70 pKa = 3.68EE71 pKa = 6.21LYY73 pKa = 9.5YY74 pKa = 10.84TGTLFWNGDD83 pKa = 3.55DD84 pKa = 3.93PEE86 pKa = 4.44EE87 pKa = 4.15EE88 pKa = 4.32FVYY91 pKa = 10.83GPLGDD96 pKa = 4.19FGMPNAGCTDD106 pKa = 2.74IRR108 pKa = 11.84YY109 pKa = 7.36TGHH112 pKa = 7.4PEE114 pKa = 3.61WDD116 pKa = 3.79CGG118 pKa = 3.79

Molecular weight: 13.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VRJ0|A0A649VRJ0_9CAUD Uncharacterized protein OS=Mycobacterium phage Blinn1 OX=2656562 GN=53 PE=4 SV=1
MM1 pKa = 6.94TWAGSRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84DD10 pKa = 3.66EE11 pKa = 5.73LPPDD15 pKa = 3.31WEE17 pKa = 4.17LKK19 pKa = 9.99YY20 pKa = 10.54RR21 pKa = 11.84LPVLSAANWLCEE33 pKa = 4.13VNGPGCVRR41 pKa = 11.84AATDD45 pKa = 3.09VDD47 pKa = 3.71HH48 pKa = 7.41KK49 pKa = 11.35KK50 pKa = 10.48RR51 pKa = 11.84GNDD54 pKa = 3.22HH55 pKa = 6.52SRR57 pKa = 11.84SNLQAICRR65 pKa = 11.84VCHH68 pKa = 5.35GRR70 pKa = 11.84KK71 pKa = 8.97SAAEE75 pKa = 3.85GVARR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84EE82 pKa = 3.88LKK84 pKa = 10.28ARR86 pKa = 11.84RR87 pKa = 11.84KK88 pKa = 9.81RR89 pKa = 11.84PPQRR93 pKa = 11.84HH94 pKa = 6.14PGQLL98 pKa = 3.22

Molecular weight: 11.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

16113

33

742

166.1

18.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.34 ± 0.316

0.931 ± 0.127

6.498 ± 0.169

7.106 ± 0.247

3.569 ± 0.169

8.819 ± 0.548

1.974 ± 0.164

5.114 ± 0.129

5.077 ± 0.242

7.77 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.259 ± 0.116

3.364 ± 0.174

5.468 ± 0.242

3.494 ± 0.181

6.051 ± 0.29

5.492 ± 0.208

5.896 ± 0.197

6.827 ± 0.22

1.998 ± 0.128

2.954 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski