Lachnospiraceae bacterium RM5

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1991 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9AAY1|A0A1H9AAY1_9FIRM Protease PrsW OS=Lachnospiraceae bacterium RM5 OX=1200721 GN=SAMN02910289_00492 PE=3 SV=1
MM1 pKa = 7.91SYY3 pKa = 10.93RR4 pKa = 11.84DD5 pKa = 3.48DD6 pKa = 3.63RR7 pKa = 11.84RR8 pKa = 11.84NKK10 pKa = 8.39EE11 pKa = 4.08TEE13 pKa = 3.55KK14 pKa = 11.23DD15 pKa = 3.33DD16 pKa = 4.41FFIEE20 pKa = 5.74FYY22 pKa = 11.29DD23 pKa = 4.43NDD25 pKa = 3.89YY26 pKa = 11.83DD27 pKa = 6.63DD28 pKa = 5.6IDD30 pKa = 4.1EE31 pKa = 4.73KK32 pKa = 11.56NSDD35 pKa = 4.11EE36 pKa = 5.17EE37 pKa = 3.91FDD39 pKa = 4.35EE40 pKa = 4.73YY41 pKa = 11.49DD42 pKa = 4.04DD43 pKa = 4.16YY44 pKa = 12.03EE45 pKa = 4.42EE46 pKa = 5.27YY47 pKa = 10.79EE48 pKa = 4.21DD49 pKa = 4.32DD50 pKa = 4.33EE51 pKa = 4.96EE52 pKa = 4.92YY53 pKa = 11.08EE54 pKa = 4.21EE55 pKa = 4.65YY56 pKa = 10.68EE57 pKa = 3.94GDD59 pKa = 3.84EE60 pKa = 4.4EE61 pKa = 4.7YY62 pKa = 11.1EE63 pKa = 4.35EE64 pKa = 4.64YY65 pKa = 10.94DD66 pKa = 3.6EE67 pKa = 6.6LDD69 pKa = 4.34DD70 pKa = 5.41EE71 pKa = 6.37DD72 pKa = 6.25YY73 pKa = 11.69DD74 pKa = 5.83DD75 pKa = 6.79DD76 pKa = 6.0YY77 pKa = 12.01DD78 pKa = 6.1DD79 pKa = 6.01YY80 pKa = 12.07DD81 pKa = 4.24DD82 pKa = 4.2EE83 pKa = 5.9PYY85 pKa = 10.61VEE87 pKa = 5.43KK88 pKa = 10.0NTKK91 pKa = 8.72VKK93 pKa = 10.69KK94 pKa = 10.47KK95 pKa = 9.08LTSNQKK101 pKa = 8.83VNRR104 pKa = 11.84AIAILSGVIFCFTAFYY120 pKa = 10.38GAIAISSFAGKK131 pKa = 8.07TKK133 pKa = 10.38AAQEE137 pKa = 4.15APADD141 pKa = 3.83NQTEE145 pKa = 4.17AEE147 pKa = 4.11EE148 pKa = 4.87GFFVSQNSDD157 pKa = 2.59ATISQEE163 pKa = 5.02IKK165 pKa = 9.91DD166 pKa = 4.19TLNSATLEE174 pKa = 4.16PLTTGYY180 pKa = 10.42EE181 pKa = 4.22AIDD184 pKa = 3.44NSVANMLKK192 pKa = 8.65VTCKK196 pKa = 10.91DD197 pKa = 4.25DD198 pKa = 3.07MTTYY202 pKa = 11.17DD203 pKa = 3.82KK204 pKa = 11.32VRR206 pKa = 11.84NIYY209 pKa = 10.59DD210 pKa = 3.39YY211 pKa = 11.78MMFTYY216 pKa = 10.21EE217 pKa = 3.86NADD220 pKa = 3.13ASFVDD225 pKa = 4.0EE226 pKa = 4.58DD227 pKa = 4.62SVYY230 pKa = 11.14DD231 pKa = 3.56FCNKK235 pKa = 9.75YY236 pKa = 10.84SYY238 pKa = 11.11TNYY241 pKa = 9.89FDD243 pKa = 4.82MEE245 pKa = 4.1MLYY248 pKa = 10.56RR249 pKa = 11.84ADD251 pKa = 4.38KK252 pKa = 11.23LMASKK257 pKa = 10.85AGDD260 pKa = 3.46SRR262 pKa = 11.84DD263 pKa = 3.71YY264 pKa = 11.57GSTFAIALRR273 pKa = 11.84ALGLDD278 pKa = 3.67AYY280 pKa = 10.54YY281 pKa = 11.02VATSSDD287 pKa = 3.44TNYY290 pKa = 10.75LAGLDD295 pKa = 3.97TIKK298 pKa = 10.86GYY300 pKa = 8.01TVVVIDD306 pKa = 3.81DD307 pKa = 3.81EE308 pKa = 5.79KK309 pKa = 11.63YY310 pKa = 9.9VFDD313 pKa = 4.81VNAEE317 pKa = 4.46DD318 pKa = 4.82IEE320 pKa = 4.73STDD323 pKa = 3.3SEE325 pKa = 4.72AGVVYY330 pKa = 10.72SFFGKK335 pKa = 10.31DD336 pKa = 3.53FLDD339 pKa = 4.2LPEE342 pKa = 4.52QYY344 pKa = 10.03TLEE347 pKa = 4.29DD348 pKa = 3.53AEE350 pKa = 4.38ASIEE354 pKa = 4.0AFGNFGTLGEE364 pKa = 4.28FGISVVATTKK374 pKa = 10.37SGEE377 pKa = 4.17YY378 pKa = 10.07SSGAVSYY385 pKa = 10.94NSSGEE390 pKa = 4.0NLIEE394 pKa = 4.97ADD396 pKa = 4.53TIGIDD401 pKa = 3.46QGEE404 pKa = 4.38TIYY407 pKa = 10.87FKK409 pKa = 10.74GTVSGSEE416 pKa = 4.0TNTWKK421 pKa = 11.02LIADD425 pKa = 4.09VYY427 pKa = 9.96DD428 pKa = 4.04HH429 pKa = 6.45NWNYY433 pKa = 7.98VTEE436 pKa = 4.16ATLYY440 pKa = 11.15NEE442 pKa = 4.21THH444 pKa = 6.52SNRR447 pKa = 11.84TDD449 pKa = 3.66EE450 pKa = 4.13ITYY453 pKa = 10.12APSRR457 pKa = 11.84GGFIVIRR464 pKa = 11.84FIVTDD469 pKa = 3.73EE470 pKa = 3.96NGRR473 pKa = 11.84TCTVKK478 pKa = 10.62FIADD482 pKa = 3.57VAGDD486 pKa = 3.79DD487 pKa = 4.51YY488 pKa = 11.7YY489 pKa = 11.4FEE491 pKa = 5.71PEE493 pKa = 3.84TTTVYY498 pKa = 8.05EE499 pKa = 4.48TTTEE503 pKa = 4.19EE504 pKa = 4.6YY505 pKa = 7.81TTEE508 pKa = 3.85EE509 pKa = 4.12VTTIEE514 pKa = 3.98EE515 pKa = 4.44TTTVEE520 pKa = 3.76EE521 pKa = 4.51TTTVEE526 pKa = 4.02EE527 pKa = 4.45TTSQEE532 pKa = 3.84EE533 pKa = 4.36TTSQEE538 pKa = 3.9EE539 pKa = 4.35SSSQEE544 pKa = 3.72EE545 pKa = 4.25TSSQEE550 pKa = 3.9EE551 pKa = 4.46TSSQTEE557 pKa = 4.49PEE559 pKa = 4.53PQPQPQPP566 pKa = 3.37

Molecular weight: 64.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9DRP7|A0A1H9DRP7_9FIRM tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG OS=Lachnospiraceae bacterium RM5 OX=1200721 GN=mnmG PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.61KK9 pKa = 7.54RR10 pKa = 11.84QRR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.37GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTASGRR28 pKa = 11.84KK29 pKa = 8.42VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1991

0

1991

697987

39

4040

350.6

39.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.783 ± 0.06

1.278 ± 0.019

6.744 ± 0.053

7.762 ± 0.066

4.295 ± 0.039

6.368 ± 0.058

1.358 ± 0.022

8.908 ± 0.058

8.853 ± 0.066

8.126 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.733 ± 0.026

6.199 ± 0.054

2.575 ± 0.027

1.964 ± 0.027

3.461 ± 0.04

6.237 ± 0.059

5.148 ± 0.076

6.639 ± 0.044

0.71 ± 0.021

4.854 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski