Amino acid dipepetide frequency for Coffea canephora (Robusta coffee)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.407AlaAla: 6.407 ± 0.039	1.293AlaCys: 1.293 ± 0.013	3.201AlaAsp: 3.201 ± 0.019	4.244AlaGlu: 4.244 ± 0.026	2.868AlaPhe: 2.868 ± 0.018	4.233AlaGly: 4.233 ± 0.024	1.278AlaHis: 1.278 ± 0.011	3.894AlaIle: 3.894 ± 0.022	3.944AlaLys: 3.944 ± 0.022	6.808AlaLeu: 6.808 ± 0.029	1.67AlaMet: 1.67 ± 0.013	2.666AlaAsn: 2.666 ± 0.019	2.657AlaPro: 2.657 ± 0.021	2.084AlaGln: 2.084 ± 0.017	3.297AlaArg: 3.297 ± 0.018	5.995AlaSer: 5.995 ± 0.03	3.525AlaThr: 3.525 ± 0.021	4.747AlaVal: 4.747 ± 0.025	0.815AlaTrp: 0.815 ± 0.009	1.88AlaTyr: 1.88 ± 0.016	0.0AlaXaa: 0.0 ± 0.0
Cys	0.982CysAla: 0.982 ± 0.011	0.568CysCys: 0.568 ± 0.009	0.906CysAsp: 0.906 ± 0.011	0.867CysGlu: 0.867 ± 0.009	0.99CysPhe: 0.99 ± 0.009	1.407CysGly: 1.407 ± 0.016	0.5CysHis: 0.5 ± 0.008	1.09CysIle: 1.09 ± 0.01	1.184CysLys: 1.184 ± 0.015	2.076CysLeu: 2.076 ± 0.015	0.455CysMet: 0.455 ± 0.007	0.884CysAsn: 0.884 ± 0.01	1.056CysPro: 1.056 ± 0.013	0.662CysGln: 0.662 ± 0.008	1.085CysArg: 1.085 ± 0.012	1.907CysSer: 1.907 ± 0.016	0.924CysThr: 0.924 ± 0.011	1.019CysVal: 1.019 ± 0.011	0.283CysTrp: 0.283 ± 0.005	0.577CysTyr: 0.577 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	3.47AspAla: 3.47 ± 0.021	1.015AspCys: 1.015 ± 0.012	3.502AspAsp: 3.502 ± 0.025	3.791AspGlu: 3.791 ± 0.023	2.468AspPhe: 2.468 ± 0.016	3.683AspGly: 3.683 ± 0.026	1.229AspHis: 1.229 ± 0.012	3.009AspIle: 3.009 ± 0.018	2.614AspLys: 2.614 ± 0.016	5.357AspLeu: 5.357 ± 0.027	1.31AspMet: 1.31 ± 0.012	2.031AspAsn: 2.031 ± 0.013	2.513AspPro: 2.513 ± 0.017	1.781AspGln: 1.781 ± 0.014	2.293AspArg: 2.293 ± 0.018	4.033AspSer: 4.033 ± 0.025	2.062AspThr: 2.062 ± 0.015	3.58AspVal: 3.58 ± 0.019	0.75AspTrp: 0.75 ± 0.009	1.557AspTyr: 1.557 ± 0.013	0.0AspXaa: 0.0 ± 0.0
Glu	4.599GluAla: 4.599 ± 0.028	0.91GluCys: 0.91 ± 0.008	3.81GluAsp: 3.81 ± 0.025	5.791GluGlu: 5.791 ± 0.042	2.392GluPhe: 2.392 ± 0.016	3.715GluGly: 3.715 ± 0.021	1.184GluHis: 1.184 ± 0.011	3.886GluIle: 3.886 ± 0.023	4.6GluLys: 4.6 ± 0.028	6.218GluLeu: 6.218 ± 0.03	1.739GluMet: 1.739 ± 0.015	3.051GluAsn: 3.051 ± 0.019	1.956GluPro: 1.956 ± 0.015	2.062GluGln: 2.062 ± 0.016	3.237GluArg: 3.237 ± 0.022	4.42GluSer: 4.42 ± 0.024	2.885GluThr: 2.885 ± 0.019	4.127GluVal: 4.127 ± 0.024	0.779GluTrp: 0.779 ± 0.009	1.626GluTyr: 1.626 ± 0.015	0.0GluXaa: 0.0 ± 0.0
Phe	2.608PheAla: 2.608 ± 0.018	1.007PheCys: 1.007 ± 0.01	2.378PheAsp: 2.378 ± 0.015	2.258PheGlu: 2.258 ± 0.016	2.183PhePhe: 2.183 ± 0.019	3.224PheGly: 3.224 ± 0.019	1.149PheHis: 1.149 ± 0.012	2.134PheIle: 2.134 ± 0.017	2.159PheLys: 2.159 ± 0.015	4.811PheLeu: 4.811 ± 0.029	0.964PheMet: 0.964 ± 0.01	1.862PheAsn: 1.862 ± 0.015	2.27PhePro: 2.27 ± 0.017	1.62PheGln: 1.62 ± 0.013	2.103PheArg: 2.103 ± 0.014	4.195PheSer: 4.195 ± 0.022	2.006PheThr: 2.006 ± 0.015	2.735PheVal: 2.735 ± 0.019	0.607PheTrp: 0.607 ± 0.008	1.334PheTyr: 1.334 ± 0.013	0.0PheXaa: 0.0 ± 0.0
Gly	3.842GlyAla: 3.842 ± 0.023	1.328GlyCys: 1.328 ± 0.014	3.252GlyAsp: 3.252 ± 0.019	3.574GlyGlu: 3.574 ± 0.019	3.19GlyPhe: 3.19 ± 0.019	5.268GlyGly: 5.268 ± 0.042	1.503GlyHis: 1.503 ± 0.014	3.835GlyIle: 3.835 ± 0.021	4.174GlyLys: 4.174 ± 0.02	6.258GlyLeu: 6.258 ± 0.029	1.531GlyMet: 1.531 ± 0.014	3.262GlyAsn: 3.262 ± 0.024	2.451GlyPro: 2.451 ± 0.017	2.146GlyGln: 2.146 ± 0.015	3.579GlyArg: 3.579 ± 0.024	5.83GlySer: 5.83 ± 0.026	3.21GlyThr: 3.21 ± 0.02	3.995GlyVal: 3.995 ± 0.024	0.897GlyTrp: 0.897 ± 0.009	2.127GlyTyr: 2.127 ± 0.018	0.0GlyXaa: 0.0 ± 0.0
His	1.382HisAla: 1.382 ± 0.013	0.551HisCys: 0.551 ± 0.007	1.174HisAsp: 1.174 ± 0.011	1.231HisGlu: 1.231 ± 0.01	1.179HisPhe: 1.179 ± 0.01	1.671HisGly: 1.671 ± 0.013	0.945HisHis: 0.945 ± 0.013	1.208HisIle: 1.208 ± 0.01	1.125HisLys: 1.125 ± 0.012	2.737HisLeu: 2.737 ± 0.02	0.535HisMet: 0.535 ± 0.007	0.954HisAsn: 0.954 ± 0.01	1.375HisPro: 1.375 ± 0.012	1.037HisGln: 1.037 ± 0.011	1.357HisArg: 1.357 ± 0.013	1.878HisSer: 1.878 ± 0.014	0.906HisThr: 0.906 ± 0.008	1.531HisVal: 1.531 ± 0.013	0.328HisTrp: 0.328 ± 0.005	0.718HisTyr: 0.718 ± 0.009	0.0HisXaa: 0.0 ± 0.0
Ile	3.677IleAla: 3.677 ± 0.022	1.172IleCys: 1.172 ± 0.011	2.86IleAsp: 2.86 ± 0.02	3.138IleGlu: 3.138 ± 0.021	2.467IlePhe: 2.467 ± 0.015	3.635IleGly: 3.635 ± 0.021	1.367IleHis: 1.367 ± 0.013	2.886IleIle: 2.886 ± 0.021	2.981IleLys: 2.981 ± 0.019	5.699IleLeu: 5.699 ± 0.024	1.139IleMet: 1.139 ± 0.011	2.216IleAsn: 2.216 ± 0.015	3.277IlePro: 3.277 ± 0.03	2.051IleGln: 2.051 ± 0.013	2.725IleArg: 2.725 ± 0.015	5.13IleSer: 5.13 ± 0.024	2.579IleThr: 2.579 ± 0.018	3.481IleVal: 3.481 ± 0.019	0.758IleTrp: 0.758 ± 0.009	1.602IleTyr: 1.602 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	3.977LysAla: 3.977 ± 0.021	1.027LysCys: 1.027 ± 0.012	3.184LysAsp: 3.184 ± 0.023	4.413LysGlu: 4.413 ± 0.028	2.303LysPhe: 2.303 ± 0.016	3.542LysGly: 3.542 ± 0.021	1.321LysHis: 1.321 ± 0.01	3.335LysIle: 3.335 ± 0.019	4.504LysLys: 4.504 ± 0.033	6.533LysLeu: 6.533 ± 0.03	1.501LysMet: 1.501 ± 0.014	2.683LysAsn: 2.683 ± 0.016	2.588LysPro: 2.588 ± 0.017	2.165LysGln: 2.165 ± 0.015	3.405LysArg: 3.405 ± 0.023	4.494LysSer: 4.494 ± 0.025	2.715LysThr: 2.715 ± 0.014	3.829LysVal: 3.829 ± 0.019	0.781LysTrp: 0.781 ± 0.008	1.635LysTyr: 1.635 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	6.904LeuAla: 6.904 ± 0.027	1.952LeuCys: 1.952 ± 0.016	5.454LeuAsp: 5.454 ± 0.025	6.878LeuGlu: 6.878 ± 0.039	4.089LeuPhe: 4.089 ± 0.024	6.1LeuGly: 6.1 ± 0.032	2.82LeuHis: 2.82 ± 0.018	5.011LeuIle: 5.011 ± 0.027	6.521LeuLys: 6.521 ± 0.03	10.648LeuLeu: 10.648 ± 0.046	2.193LeuMet: 2.193 ± 0.015	4.161LeuAsn: 4.161 ± 0.022	5.61LeuPro: 5.61 ± 0.03	4.705LeuGln: 4.705 ± 0.029	5.831LeuArg: 5.831 ± 0.031	9.33LeuSer: 9.33 ± 0.06	4.71LeuThr: 4.71 ± 0.031	6.694LeuVal: 6.694 ± 0.029	1.248LeuTrp: 1.248 ± 0.012	2.733LeuTyr: 2.733 ± 0.017	0.0LeuXaa: 0.0 ± 0.0
Met	2.063MetAla: 2.063 ± 0.016	0.328MetCys: 0.328 ± 0.006	1.369MetAsp: 1.369 ± 0.011	1.886MetGlu: 1.886 ± 0.014	0.811MetPhe: 0.811 ± 0.009	1.548MetGly: 1.548 ± 0.013	0.571MetHis: 0.571 ± 0.007	1.246MetIle: 1.246 ± 0.012	1.579MetLys: 1.579 ± 0.013	2.26MetLeu: 2.26 ± 0.014	0.627MetMet: 0.627 ± 0.008	0.974MetAsn: 0.974 ± 0.01	1.081MetPro: 1.081 ± 0.011	0.915MetGln: 0.915 ± 0.01	1.177MetArg: 1.177 ± 0.012	1.662MetSer: 1.662 ± 0.013	1.036MetThr: 1.036 ± 0.01	1.607MetVal: 1.607 ± 0.014	0.258MetTrp: 0.258 ± 0.005	0.58MetTyr: 0.58 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	2.693AsnAla: 2.693 ± 0.017	0.971AsnCys: 0.971 ± 0.011	2.024AsnAsp: 2.024 ± 0.015	2.407AsnGlu: 2.407 ± 0.018	2.14AsnPhe: 2.14 ± 0.015	3.17AsnGly: 3.17 ± 0.02	1.112AsnHis: 1.112 ± 0.01	2.489AsnIle: 2.489 ± 0.018	2.244AsnLys: 2.244 ± 0.017	5.49AsnLeu: 5.49 ± 0.048	1.061AsnMet: 1.061 ± 0.011	2.165AsnAsn: 2.165 ± 0.026	2.308AsnPro: 2.308 ± 0.016	1.764AsnGln: 1.764 ± 0.015	1.997AsnArg: 1.997 ± 0.012	3.918AsnSer: 3.918 ± 0.023	1.8AsnThr: 1.8 ± 0.014	2.685AsnVal: 2.685 ± 0.017	0.601AsnTrp: 0.601 ± 0.008	1.335AsnTyr: 1.335 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	3.056ProAla: 3.056 ± 0.023	0.803ProCys: 0.803 ± 0.009	2.515ProAsp: 2.515 ± 0.014	3.165ProGlu: 3.165 ± 0.021	2.014ProPhe: 2.014 ± 0.013	2.687ProGly: 2.687 ± 0.018	1.134ProHis: 1.134 ± 0.011	2.319ProIle: 2.319 ± 0.015	2.733ProLys: 2.733 ± 0.017	4.357ProLeu: 4.357 ± 0.02	0.916ProMet: 0.916 ± 0.009	2.309ProAsn: 2.309 ± 0.017	3.73ProPro: 3.73 ± 0.057	1.819ProGln: 1.819 ± 0.02	2.336ProArg: 2.336 ± 0.019	5.004ProSer: 5.004 ± 0.029	2.485ProThr: 2.485 ± 0.019	2.992ProVal: 2.992 ± 0.022	0.594ProTrp: 0.594 ± 0.008	1.315ProTyr: 1.315 ± 0.013	0.0ProXaa: 0.0 ± 0.0
Gln	2.368GlnAla: 2.368 ± 0.017	0.584GlnCys: 0.584 ± 0.008	1.677GlnAsp: 1.677 ± 0.013	2.415GlnGlu: 2.415 ± 0.015	1.491GlnPhe: 1.491 ± 0.013	2.083GlnGly: 2.083 ± 0.015	0.934GlnHis: 0.934 ± 0.009	2.111GlnIle: 2.111 ± 0.015	2.397GlnLys: 2.397 ± 0.017	3.958GlnLeu: 3.958 ± 0.023	0.967GlnMet: 0.967 ± 0.01	1.888GlnAsn: 1.888 ± 0.015	1.68GlnPro: 1.68 ± 0.015	1.944GlnGln: 1.944 ± 0.028	2.026GlnArg: 2.026 ± 0.014	2.778GlnSer: 2.778 ± 0.021	1.675GlnThr: 1.675 ± 0.014	2.374GlnVal: 2.374 ± 0.016	0.457GlnTrp: 0.457 ± 0.007	0.952GlnTyr: 0.952 ± 0.01	0.0GlnXaa: 0.0 ± 0.0
Arg	3.201ArgAla: 3.201 ± 0.02	0.99ArgCys: 0.99 ± 0.012	2.592ArgAsp: 2.592 ± 0.018	3.255ArgGlu: 3.255 ± 0.022	2.165ArgPhe: 2.165 ± 0.014	3.127ArgGly: 3.127 ± 0.019	1.312ArgHis: 1.312 ± 0.012	3.013ArgIle: 3.013 ± 0.018	3.649ArgLys: 3.649 ± 0.021	5.285ArgLeu: 5.285 ± 0.023	1.306ArgMet: 1.306 ± 0.011	2.486ArgAsn: 2.486 ± 0.018	2.193ArgPro: 2.193 ± 0.016	1.829ArgGln: 1.829 ± 0.014	3.789ArgArg: 3.789 ± 0.026	4.155ArgSer: 4.155 ± 0.026	2.4ArgThr: 2.4 ± 0.017	3.21ArgVal: 3.21 ± 0.019	0.737ArgTrp: 0.737 ± 0.009	1.503ArgTyr: 1.503 ± 0.013	0.0ArgXaa: 0.0 ± 0.0
Ser	5.4SerAla: 5.4 ± 0.031	1.84SerCys: 1.84 ± 0.013	4.19SerAsp: 4.19 ± 0.019	4.564SerGlu: 4.564 ± 0.025	4.181SerPhe: 4.181 ± 0.024	5.894SerGly: 5.894 ± 0.032	1.948SerHis: 1.948 ± 0.014	4.85SerIle: 4.85 ± 0.024	4.657SerLys: 4.657 ± 0.028	9.309SerLeu: 9.309 ± 0.054	2.014SerMet: 2.014 ± 0.014	4.061SerAsn: 4.061 ± 0.024	4.265SerPro: 4.265 ± 0.031	2.982SerGln: 2.982 ± 0.02	4.429SerArg: 4.429 ± 0.024	10.751SerSer: 10.751 ± 0.05	4.611SerThr: 4.611 ± 0.025	5.01SerVal: 5.01 ± 0.024	1.254SerTrp: 1.254 ± 0.013	2.33SerTyr: 2.33 ± 0.015	0.0SerXaa: 0.0 ± 0.0
Thr	3.41ThrAla: 3.41 ± 0.021	0.97ThrCys: 0.97 ± 0.009	2.186ThrAsp: 2.186 ± 0.015	2.634ThrGlu: 2.634 ± 0.017	2.066ThrPhe: 2.066 ± 0.014	3.253ThrGly: 3.253 ± 0.018	0.956ThrHis: 0.956 ± 0.01	2.795ThrIle: 2.795 ± 0.017	2.568ThrLys: 2.568 ± 0.016	4.797ThrLeu: 4.797 ± 0.025	1.089ThrMet: 1.089 ± 0.01	2.021ThrAsn: 2.021 ± 0.016	2.405ThrPro: 2.405 ± 0.019	1.416ThrGln: 1.416 ± 0.012	2.297ThrArg: 2.297 ± 0.016	4.532ThrSer: 4.532 ± 0.025	2.914ThrThr: 2.914 ± 0.02	3.169ThrVal: 3.169 ± 0.019	0.63ThrTrp: 0.63 ± 0.009	1.363ThrTyr: 1.363 ± 0.012	0.0ThrXaa: 0.0 ± 0.0
Val	4.853ValAla: 4.853 ± 0.026	1.14ValCys: 1.14 ± 0.014	3.546ValAsp: 3.546 ± 0.02	4.157ValGlu: 4.157 ± 0.024	2.684ValPhe: 2.684 ± 0.017	4.132ValGly: 4.132 ± 0.02	1.524ValHis: 1.524 ± 0.013	3.444ValIle: 3.444 ± 0.018	3.851ValLys: 3.851 ± 0.021	6.566ValLeu: 6.566 ± 0.03	1.457ValMet: 1.457 ± 0.011	2.569ValAsn: 2.569 ± 0.018	3.068ValPro: 3.068 ± 0.021	2.328ValGln: 2.328 ± 0.015	2.993ValArg: 2.993 ± 0.017	5.211ValSer: 5.211 ± 0.021	3.064ValThr: 3.064 ± 0.019	4.716ValVal: 4.716 ± 0.028	0.776ValTrp: 0.776 ± 0.01	1.977ValTyr: 1.977 ± 0.013	0.0ValXaa: 0.0 ± 0.0
Trp	0.803TrpAla: 0.803 ± 0.009	0.26TrpCys: 0.26 ± 0.005	0.715TrpAsp: 0.715 ± 0.008	0.783TrpGlu: 0.783 ± 0.01	0.547TrpPhe: 0.547 ± 0.007	0.749TrpGly: 0.749 ± 0.01	0.294TrpHis: 0.294 ± 0.005	0.788TrpIle: 0.788 ± 0.009	0.947TrpLys: 0.947 ± 0.009	1.326TrpLeu: 1.326 ± 0.013	0.363TrpMet: 0.363 ± 0.006	0.762TrpAsn: 0.762 ± 0.009	0.513TrpPro: 0.513 ± 0.008	0.459TrpGln: 0.459 ± 0.006	0.807TrpArg: 0.807 ± 0.009	1.015TrpSer: 1.015 ± 0.011	0.671TrpThr: 0.671 ± 0.009	0.804TrpVal: 0.804 ± 0.01	0.234TrpTrp: 0.234 ± 0.006	0.363TrpTyr: 0.363 ± 0.007	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.809TyrAla: 1.809 ± 0.014	0.713TyrCys: 0.713 ± 0.009	1.512TyrAsp: 1.512 ± 0.012	1.536TyrGlu: 1.536 ± 0.013	1.381TyrPhe: 1.381 ± 0.013	2.133TyrGly: 2.133 ± 0.015	0.744TyrHis: 0.744 ± 0.009	1.494TyrIle: 1.494 ± 0.016	1.504TyrLys: 1.504 ± 0.013	3.099TyrLeu: 3.099 ± 0.022	0.74TyrMet: 0.74 ± 0.008	1.371TyrAsn: 1.371 ± 0.014	1.287TyrPro: 1.287 ± 0.012	0.988TyrGln: 0.988 ± 0.01	1.436TyrArg: 1.436 ± 0.013	2.303TyrSer: 2.303 ± 0.015	1.291TyrThr: 1.291 ± 0.013	1.75TyrVal: 1.75 ± 0.015	0.416TyrTrp: 0.416 ± 0.007	1.027TyrTyr: 1.027 ± 0.012	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.001

Statistics based on 25401 proteins (10225770 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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