Denitrobaculum tricleocarpae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Denitrobaculum

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5784 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A545TSZ1|A0A545TSZ1_9PROT Uncharacterized protein OS=Denitrobaculum tricleocarpae OX=2591009 GN=FKG95_09075 PE=4 SV=1
MM1 pKa = 6.82VAPVFFNQYY10 pKa = 10.02FADD13 pKa = 4.06KK14 pKa = 11.14NNGVHH19 pKa = 6.88DD20 pKa = 4.42WSSHH24 pKa = 3.75TFRR27 pKa = 11.84VAFCNAANAPVVGNSVLANLTTVSEE52 pKa = 4.33STIASTVVTVTSSTQTAGVWSAVLADD78 pKa = 4.14RR79 pKa = 11.84DD80 pKa = 3.93IVVTGTMPTWRR91 pKa = 11.84YY92 pKa = 8.72IVLYY96 pKa = 9.74NDD98 pKa = 3.86TPTTPADD105 pKa = 3.5PLVYY109 pKa = 10.4FIDD112 pKa = 3.61TGVDD116 pKa = 3.29NNITDD121 pKa = 3.63DD122 pKa = 3.8TYY124 pKa = 11.24PIDD127 pKa = 3.62FAAALTLSEE136 pKa = 4.18QAAA139 pKa = 3.64

Molecular weight: 14.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A545U1E6|A0A545U1E6_9PROT LysR family transcriptional regulator OS=Denitrobaculum tricleocarpae OX=2591009 GN=FKG95_01540 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLVRR12 pKa = 11.84KK13 pKa = 9.31RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.5GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.81ATVGGRR28 pKa = 11.84KK29 pKa = 9.44VLVARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.79GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5784

0

5784

1843579

25

4098

318.7

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.433 ± 0.043

0.952 ± 0.011

5.835 ± 0.032

6.431 ± 0.028

3.906 ± 0.023

8.335 ± 0.045

1.951 ± 0.015

5.303 ± 0.024

3.562 ± 0.028

10.307 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.018

2.825 ± 0.018

4.865 ± 0.025

3.322 ± 0.017

6.481 ± 0.032

6.056 ± 0.024

5.18 ± 0.021

7.236 ± 0.026

1.301 ± 0.014

2.306 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski