Peanut chlorotic fan-spot virus

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9YUD6|Q9YUD6_9VIRU Nonstructural protein NSs OS=Peanut chlorotic fan-spot virus OX=80271 PE=4 SV=1
MM1 pKa = 7.0STEE4 pKa = 3.96SSNPGSTEE12 pKa = 3.61NVALIPEE19 pKa = 4.72PFDD22 pKa = 3.33EE23 pKa = 4.79QYY25 pKa = 11.04CEE27 pKa = 4.02EE28 pKa = 4.58ALTSYY33 pKa = 11.03GNNLDD38 pKa = 3.4KK39 pKa = 11.17SALVDD44 pKa = 3.78VYY46 pKa = 11.9SMFEE50 pKa = 3.81DD51 pKa = 4.07DD52 pKa = 5.31KK53 pKa = 11.8LVFQSIHH60 pKa = 5.14ATNGRR65 pKa = 11.84FKK67 pKa = 9.88ATCAFGHH74 pKa = 6.36SDD76 pKa = 3.28MVLDD80 pKa = 5.13SEE82 pKa = 5.06QEE84 pKa = 4.12LKK86 pKa = 10.35TSISSEE92 pKa = 3.75IFHH95 pKa = 6.4QEE97 pKa = 2.75FDD99 pKa = 4.4AISGINSIISLQGEE113 pKa = 4.5NIHH116 pKa = 6.64VIISHH121 pKa = 6.9PEE123 pKa = 3.8QKK125 pKa = 10.46VKK127 pKa = 10.14SYY129 pKa = 10.82KK130 pKa = 9.61YY131 pKa = 9.85AFHH134 pKa = 6.96GRR136 pKa = 11.84IASNDD141 pKa = 3.26VFPRR145 pKa = 11.84ISGFDD150 pKa = 3.46EE151 pKa = 4.44FRR153 pKa = 11.84SQYY156 pKa = 10.64SCNPYY161 pKa = 10.22KK162 pKa = 10.37FVYY165 pKa = 9.33NPGMLGLEE173 pKa = 4.42SEE175 pKa = 4.8TNTVIFPVEE184 pKa = 3.9KK185 pKa = 10.67LGTLPAHH192 pKa = 7.01ASAFGNNFDD201 pKa = 3.93RR202 pKa = 11.84CHH204 pKa = 6.58FSGSSVPGFMTVKK217 pKa = 10.33AVSEE221 pKa = 4.33SSKK224 pKa = 9.56RR225 pKa = 11.84TQEE228 pKa = 3.77KK229 pKa = 10.03AHH231 pKa = 6.19RR232 pKa = 11.84QLHH235 pKa = 5.33NKK237 pKa = 7.96SLKK240 pKa = 10.39ALEE243 pKa = 4.34IVSNSDD249 pKa = 3.09FRR251 pKa = 11.84STYY254 pKa = 7.99ITKK257 pKa = 8.01TTLKK261 pKa = 10.24ARR263 pKa = 11.84KK264 pKa = 8.7NGMMTIQVQVKK275 pKa = 9.83ISTASLKK282 pKa = 10.32RR283 pKa = 11.84EE284 pKa = 3.95HH285 pKa = 7.04LIAIPDD291 pKa = 4.04DD292 pKa = 3.72PLKK295 pKa = 10.92RR296 pKa = 11.84LVYY299 pKa = 10.41CSSEE303 pKa = 4.17CLGQTSPGLSTFIFEE318 pKa = 5.27VMCLDD323 pKa = 4.02CLVDD327 pKa = 4.43RR328 pKa = 11.84VTPDD332 pKa = 2.92VTYY335 pKa = 10.11FAGALSTCFTDD346 pKa = 4.15EE347 pKa = 4.6PPHH350 pKa = 7.2ILLKK354 pKa = 10.59ARR356 pKa = 11.84SGILSSLADD365 pKa = 3.62SDD367 pKa = 3.49RR368 pKa = 11.84DD369 pKa = 3.7KK370 pKa = 11.29INEE373 pKa = 4.31IICKK377 pKa = 9.95NLVSVHH383 pKa = 6.43LGLATDD389 pKa = 4.77LAKK392 pKa = 10.84KK393 pKa = 10.03LNKK396 pKa = 9.04PINVFTIKK404 pKa = 9.74DD405 pKa = 3.9TSSMTTDD412 pKa = 3.12MVEE415 pKa = 4.04VDD417 pKa = 3.33GKK419 pKa = 10.67KK420 pKa = 10.5YY421 pKa = 10.26RR422 pKa = 11.84VLKK425 pKa = 10.61DD426 pKa = 3.43SNGDD430 pKa = 3.6YY431 pKa = 10.83YY432 pKa = 10.43FTSATFEE439 pKa = 3.99KK440 pKa = 10.13TLIGAYY446 pKa = 9.41KK447 pKa = 9.98SCQTFVDD454 pKa = 3.74YY455 pKa = 11.2CDD457 pKa = 3.63NKK459 pKa = 10.59KK460 pKa = 10.88LSINGDD466 pKa = 3.1NVFIFNN472 pKa = 4.41

Molecular weight: 52.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9YUD6|Q9YUD6_9VIRU Nonstructural protein NSs OS=Peanut chlorotic fan-spot virus OX=80271 PE=4 SV=1
MM1 pKa = 7.6SKK3 pKa = 9.31TKK5 pKa = 10.66VKK7 pKa = 10.5NDD9 pKa = 3.16KK10 pKa = 10.52EE11 pKa = 4.27LFEE14 pKa = 4.37SLSRR18 pKa = 11.84NAKK21 pKa = 9.74IEE23 pKa = 3.85LSQEE27 pKa = 3.76QANFTFKK34 pKa = 10.89KK35 pKa = 10.26FFSEE39 pKa = 4.04NNSKK43 pKa = 10.55VEE45 pKa = 3.79ISDD48 pKa = 3.31EE49 pKa = 4.19NMILFINNSNKK60 pKa = 8.95MKK62 pKa = 10.7AIGRR66 pKa = 11.84EE67 pKa = 4.15SSVSKK72 pKa = 10.79YY73 pKa = 10.2LGHH76 pKa = 6.76SIVKK80 pKa = 10.31SSPGVNEE87 pKa = 4.21FTWSRR92 pKa = 11.84MDD94 pKa = 3.2SLIRR98 pKa = 11.84MKK100 pKa = 10.78YY101 pKa = 8.12IEE103 pKa = 4.23RR104 pKa = 11.84VKK106 pKa = 10.86DD107 pKa = 3.55YY108 pKa = 11.55GDD110 pKa = 4.13DD111 pKa = 3.49RR112 pKa = 11.84MKK114 pKa = 11.2AEE116 pKa = 4.09NAKK119 pKa = 8.65LTNWLLEE126 pKa = 4.06VFNLHH131 pKa = 6.06TDD133 pKa = 3.11AMSPRR138 pKa = 11.84DD139 pKa = 3.51EE140 pKa = 5.1VIFKK144 pKa = 10.75VVTGGNLNHH153 pKa = 6.95LMGFKK158 pKa = 8.22TTFAHH163 pKa = 7.16AFAIANYY170 pKa = 5.88QHH172 pKa = 6.58KK173 pKa = 10.19RR174 pKa = 11.84SEE176 pKa = 3.93EE177 pKa = 3.78LGIKK181 pKa = 10.49NFDD184 pKa = 3.3TKK186 pKa = 11.2AQLDD190 pKa = 3.73RR191 pKa = 11.84MVVIGEE197 pKa = 4.24RR198 pKa = 11.84CSLLPSPTPKK208 pKa = 10.03TLIEE212 pKa = 4.42SYY214 pKa = 10.43FRR216 pKa = 11.84NAVPKK221 pKa = 10.47AKK223 pKa = 10.03SDD225 pKa = 4.1SKK227 pKa = 11.27DD228 pKa = 3.22QSSKK232 pKa = 11.55YY233 pKa = 10.59NDD235 pKa = 3.72LQNAIAAVNFEE246 pKa = 4.43VPWRR250 pKa = 11.84QVMLYY255 pKa = 9.78QVLNIYY261 pKa = 10.38LLIVKK266 pKa = 10.18AFLFF270 pKa = 4.32

Molecular weight: 31.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

742

270

472

371.0

41.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.795 ± 0.298

1.752 ± 0.822

5.795 ± 0.584

6.065 ± 0.358

6.065 ± 0.138

4.447 ± 0.663

2.426 ± 0.342

6.334 ± 0.243

8.221 ± 1.059

8.086 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.6

6.334 ± 1.08

3.235 ± 0.382

2.83 ± 0.079

3.639 ± 0.48

10.377 ± 0.666

5.795 ± 1.024

6.469 ± 0.118

0.404 ± 0.421

3.235 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski