Amino acid dipepetide frequency for Staphylothermus marinus (strain ATCC 43588 / DSM 3639 / JCM 9404 / F1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.564AlaAla: 3.564 ± 0.119
0.33AlaCys: 0.33 ± 0.03
2.313AlaAsp: 2.313 ± 0.077
3.936AlaGlu: 3.936 ± 0.117
2.306AlaPhe: 2.306 ± 0.075
4.175AlaGly: 4.175 ± 0.097
0.901AlaHis: 0.901 ± 0.046
5.286AlaIle: 5.286 ± 0.103
4.204AlaLys: 4.204 ± 0.097
6.902AlaLeu: 6.902 ± 0.139
1.315AlaMet: 1.315 ± 0.055
1.781AlaAsn: 1.781 ± 0.066
2.128AlaPro: 2.128 ± 0.078
0.932AlaGln: 0.932 ± 0.046
3.472AlaArg: 3.472 ± 0.096
3.661AlaSer: 3.661 ± 0.097
2.891AlaThr: 2.891 ± 0.082
4.417AlaVal: 4.417 ± 0.095
0.796AlaTrp: 0.796 ± 0.048
3.03AlaTyr: 3.03 ± 0.074
0.0AlaXaa: 0.0 ± 0.0
Cys
0.213CysAla: 0.213 ± 0.024
0.064CysCys: 0.064 ± 0.012
0.27CysAsp: 0.27 ± 0.022
0.259CysGlu: 0.259 ± 0.023
0.257CysPhe: 0.257 ± 0.024
0.732CysGly: 0.732 ± 0.052
0.117CysHis: 0.117 ± 0.015
0.569CysIle: 0.569 ± 0.04
0.378CysLys: 0.378 ± 0.029
0.547CysLeu: 0.547 ± 0.035
0.167CysMet: 0.167 ± 0.022
0.266CysAsn: 0.266 ± 0.028
0.6CysPro: 0.6 ± 0.046
0.09CysGln: 0.09 ± 0.015
0.466CysArg: 0.466 ± 0.034
0.416CysSer: 0.416 ± 0.033
0.224CysThr: 0.224 ± 0.025
0.314CysVal: 0.314 ± 0.028
0.084CysTrp: 0.084 ± 0.014
0.251CysTyr: 0.251 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
2.579AspAla: 2.579 ± 0.084
0.189AspCys: 0.189 ± 0.02
1.746AspAsp: 1.746 ± 0.072
3.566AspGlu: 3.566 ± 0.098
1.451AspPhe: 1.451 ± 0.058
2.304AspGly: 2.304 ± 0.079
0.864AspHis: 0.864 ± 0.047
4.949AspIle: 4.949 ± 0.111
4.305AspLys: 4.305 ± 0.101
5.396AspLeu: 5.396 ± 0.114
0.954AspMet: 0.954 ± 0.051
1.933AspAsn: 1.933 ± 0.061
3.186AspPro: 3.186 ± 0.086
0.954AspGln: 0.954 ± 0.052
2.056AspArg: 2.056 ± 0.067
1.931AspSer: 1.931 ± 0.061
2.117AspThr: 2.117 ± 0.07
3.054AspVal: 3.054 ± 0.091
0.624AspTrp: 0.624 ± 0.037
2.542AspTyr: 2.542 ± 0.081
0.0AspXaa: 0.0 ± 0.0
Glu
4.941GluAla: 4.941 ± 0.112
0.356GluCys: 0.356 ± 0.031
2.9GluAsp: 2.9 ± 0.093
6.464GluGlu: 6.464 ± 0.142
2.137GluPhe: 2.137 ± 0.069
3.241GluGly: 3.241 ± 0.086
1.176GluHis: 1.176 ± 0.051
6.897GluIle: 6.897 ± 0.165
7.799GluLys: 7.799 ± 0.162
7.007GluLeu: 7.007 ± 0.133
1.249GluMet: 1.249 ± 0.056
3.342GluAsn: 3.342 ± 0.093
2.502GluPro: 2.502 ± 0.092
1.383GluGln: 1.383 ± 0.057
2.718GluArg: 2.718 ± 0.079
2.491GluSer: 2.491 ± 0.079
3.294GluThr: 3.294 ± 0.075
3.993GluVal: 3.993 ± 0.085
0.825GluTrp: 0.825 ± 0.047
3.698GluTyr: 3.698 ± 0.093
0.0GluXaa: 0.0 ± 0.0
Phe
1.805PheAla: 1.805 ± 0.072
0.22PheCys: 0.22 ± 0.021
1.689PheAsp: 1.689 ± 0.062
1.911PheGlu: 1.911 ± 0.06
1.409PhePhe: 1.409 ± 0.06
1.992PheGly: 1.992 ± 0.073
0.602PheHis: 0.602 ± 0.038
4.437PheIle: 4.437 ± 0.122
2.579PheLys: 2.579 ± 0.07
3.912PheLeu: 3.912 ± 0.106
0.908PheMet: 0.908 ± 0.047
1.759PheAsn: 1.759 ± 0.075
1.262PhePro: 1.262 ± 0.055
0.673PheGln: 0.673 ± 0.038
1.816PheArg: 1.816 ± 0.074
2.397PheSer: 2.397 ± 0.079
1.898PheThr: 1.898 ± 0.07
2.265PheVal: 2.265 ± 0.072
0.391PheTrp: 0.391 ± 0.031
1.843PheTyr: 1.843 ± 0.069
0.0PheXaa: 0.0 ± 0.0
Gly
3.602GlyAla: 3.602 ± 0.103
0.545GlyCys: 0.545 ± 0.042
2.902GlyAsp: 2.902 ± 0.086
4.343GlyGlu: 4.343 ± 0.108
3.423GlyPhe: 3.423 ± 0.105
4.252GlyGly: 4.252 ± 0.128
1.02GlyHis: 1.02 ± 0.055
6.28GlyIle: 6.28 ± 0.124
5.046GlyLys: 5.046 ± 0.106
7.245GlyLeu: 7.245 ± 0.157
1.33GlyMet: 1.33 ± 0.045
1.966GlyAsn: 1.966 ± 0.067
1.909GlyPro: 1.909 ± 0.069
0.926GlyGln: 0.926 ± 0.048
3.454GlyArg: 3.454 ± 0.079
3.465GlySer: 3.465 ± 0.103
2.603GlyThr: 2.603 ± 0.077
5.455GlyVal: 5.455 ± 0.118
0.965GlyTrp: 0.965 ± 0.052
3.802GlyTyr: 3.802 ± 0.096
0.0GlyXaa: 0.0 ± 0.0
His
0.963HisAla: 0.963 ± 0.048
0.15HisCys: 0.15 ± 0.018
0.816HisAsp: 0.816 ± 0.041
1.058HisGlu: 1.058 ± 0.043
0.475HisPhe: 0.475 ± 0.032
1.722HisGly: 1.722 ± 0.065
0.431HisHis: 0.431 ± 0.034
1.737HisIle: 1.737 ± 0.069
1.088HisLys: 1.088 ± 0.043
1.563HisLeu: 1.563 ± 0.071
0.376HisMet: 0.376 ± 0.03
0.664HisAsn: 0.664 ± 0.035
0.994HisPro: 0.994 ± 0.054
0.264HisGln: 0.264 ± 0.028
1.15HisArg: 1.15 ± 0.046
0.858HisSer: 0.858 ± 0.046
0.743HisThr: 0.743 ± 0.04
1.394HisVal: 1.394 ± 0.057
0.209HisTrp: 0.209 ± 0.019
0.853HisTyr: 0.853 ± 0.041
0.0HisXaa: 0.0 ± 0.0
Ile
6.592IleAla: 6.592 ± 0.13
0.624IleCys: 0.624 ± 0.039
6.363IleAsp: 6.363 ± 0.129
6.576IleGlu: 6.576 ± 0.125
2.907IlePhe: 2.907 ± 0.096
6.816IleGly: 6.816 ± 0.153
1.878IleHis: 1.878 ± 0.063
11.51IleIle: 11.51 ± 0.209
6.324IleLys: 6.324 ± 0.107
10.007IleLeu: 10.007 ± 0.21
2.309IleMet: 2.309 ± 0.073
5.059IleAsn: 5.059 ± 0.135
4.49IlePro: 4.49 ± 0.103
1.55IleGln: 1.55 ± 0.062
5.246IleArg: 5.246 ± 0.126
5.853IleSer: 5.853 ± 0.145
4.908IleThr: 4.908 ± 0.114
8.962IleVal: 8.962 ± 0.143
0.895IleTrp: 0.895 ± 0.048
5.191IleTyr: 5.191 ± 0.123
0.0IleXaa: 0.0 ± 0.0
Lys
4.49LysAla: 4.49 ± 0.104
0.688LysCys: 0.688 ± 0.047
2.858LysAsp: 2.858 ± 0.078
5.088LysGlu: 5.088 ± 0.104
1.891LysPhe: 1.891 ± 0.061
4.059LysGly: 4.059 ± 0.097
1.891LysHis: 1.891 ± 0.075
8.382LysIle: 8.382 ± 0.133
5.981LysLys: 5.981 ± 0.148
8.008LysLeu: 8.008 ± 0.141
1.766LysMet: 1.766 ± 0.06
3.393LysAsn: 3.393 ± 0.08
4.167LysPro: 4.167 ± 0.097
1.735LysGln: 1.735 ± 0.063
3.542LysArg: 3.542 ± 0.095
3.021LysSer: 3.021 ± 0.088
4.303LysThr: 4.303 ± 0.106
4.279LysVal: 4.279 ± 0.113
0.818LysTrp: 0.818 ± 0.045
4.815LysTyr: 4.815 ± 0.106
0.0LysXaa: 0.0 ± 0.0
Leu
6.858LeuAla: 6.858 ± 0.142
0.475LeuCys: 0.475 ± 0.032
5.182LeuAsp: 5.182 ± 0.119
7.542LeuGlu: 7.542 ± 0.138
4.107LeuPhe: 4.107 ± 0.129
7.054LeuGly: 7.054 ± 0.16
1.605LeuHis: 1.605 ± 0.061
9.307LeuIle: 9.307 ± 0.19
7.328LeuLys: 7.328 ± 0.141
11.381LeuLeu: 11.381 ± 0.216
2.223LeuMet: 2.223 ± 0.086
3.907LeuAsn: 3.907 ± 0.105
3.951LeuPro: 3.951 ± 0.097
1.724LeuGln: 1.724 ± 0.065
6.077LeuArg: 6.077 ± 0.129
7.302LeuSer: 7.302 ± 0.149
4.576LeuThr: 4.576 ± 0.1
7.612LeuVal: 7.612 ± 0.122
1.126LeuTrp: 1.126 ± 0.055
4.602LeuTyr: 4.602 ± 0.104
0.0LeuXaa: 0.0 ± 0.0
Met
1.321MetAla: 1.321 ± 0.046
0.147MetCys: 0.147 ± 0.019
1.049MetAsp: 1.049 ± 0.044
1.348MetGlu: 1.348 ± 0.052
0.847MetPhe: 0.847 ± 0.044
1.605MetGly: 1.605 ± 0.056
0.402MetHis: 0.402 ± 0.03
2.216MetIle: 2.216 ± 0.074
1.75MetLys: 1.75 ± 0.064
2.361MetLeu: 2.361 ± 0.08
0.477MetMet: 0.477 ± 0.031
1.194MetAsn: 1.194 ± 0.053
0.974MetPro: 0.974 ± 0.049
0.363MetGln: 0.363 ± 0.026
1.185MetArg: 1.185 ± 0.055
1.583MetSer: 1.583 ± 0.06
0.886MetThr: 0.886 ± 0.05
1.55MetVal: 1.55 ± 0.061
0.193MetTrp: 0.193 ± 0.021
0.954MetTyr: 0.954 ± 0.042
0.0MetXaa: 0.0 ± 0.0
Asn
2.177AsnAla: 2.177 ± 0.084
0.257AsnCys: 0.257 ± 0.022
1.847AsnAsp: 1.847 ± 0.063
2.557AsnGlu: 2.557 ± 0.078
0.928AsnPhe: 0.928 ± 0.047
2.405AsnGly: 2.405 ± 0.087
0.6AsnHis: 0.6 ± 0.037
5.706AsnIle: 5.706 ± 0.122
3.553AsnLys: 3.553 ± 0.099
3.514AsnLeu: 3.514 ± 0.099
0.994AsnMet: 0.994 ± 0.046
2.951AsnAsn: 2.951 ± 0.095
2.311AsnPro: 2.311 ± 0.077
0.631AsnGln: 0.631 ± 0.046
1.702AsnArg: 1.702 ± 0.053
1.941AsnSer: 1.941 ± 0.075
2.557AsnThr: 2.557 ± 0.09
2.922AsnVal: 2.922 ± 0.081
0.433AsnTrp: 0.433 ± 0.035
2.346AsnTyr: 2.346 ± 0.082
0.0AsnXaa: 0.0 ± 0.0
Pro
2.069ProAla: 2.069 ± 0.068
0.281ProCys: 0.281 ± 0.023
2.355ProAsp: 2.355 ± 0.081
3.27ProGlu: 3.27 ± 0.112
1.535ProPhe: 1.535 ± 0.055
3.265ProGly: 3.265 ± 0.099
0.895ProHis: 0.895 ± 0.04
3.799ProIle: 3.799 ± 0.098
2.401ProLys: 2.401 ± 0.072
4.571ProLeu: 4.571 ± 0.1
0.765ProMet: 0.765 ± 0.038
1.458ProAsn: 1.458 ± 0.06
2.438ProPro: 2.438 ± 0.078
0.934ProGln: 0.934 ± 0.047
2.599ProArg: 2.599 ± 0.068
2.667ProSer: 2.667 ± 0.066
1.884ProThr: 1.884 ± 0.07
3.386ProVal: 3.386 ± 0.1
0.754ProTrp: 0.754 ± 0.044
2.304ProTyr: 2.304 ± 0.071
0.0ProXaa: 0.0 ± 0.0
Gln
1.227GlnAla: 1.227 ± 0.063
0.119GlnCys: 0.119 ± 0.016
0.589GlnAsp: 0.589 ± 0.038
1.088GlnGlu: 1.088 ± 0.048
0.523GlnPhe: 0.523 ± 0.034
1.095GlnGly: 1.095 ± 0.044
0.332GlnHis: 0.332 ± 0.031
1.645GlnIle: 1.645 ± 0.057
1.106GlnLys: 1.106 ± 0.055
2.148GlnLeu: 2.148 ± 0.075
0.42GlnMet: 0.42 ± 0.031
0.655GlnAsn: 0.655 ± 0.04
0.774GlnPro: 0.774 ± 0.042
0.556GlnGln: 0.556 ± 0.045
0.989GlnArg: 0.989 ± 0.047
0.796GlnSer: 0.796 ± 0.043
0.886GlnThr: 0.886 ± 0.044
1.31GlnVal: 1.31 ± 0.051
0.202GlnTrp: 0.202 ± 0.022
0.974GlnTyr: 0.974 ± 0.043
0.0GlnXaa: 0.0 ± 0.0
Arg
2.687ArgAla: 2.687 ± 0.082
0.418ArgCys: 0.418 ± 0.042
2.759ArgAsp: 2.759 ± 0.086
4.802ArgGlu: 4.802 ± 0.126
1.97ArgPhe: 1.97 ± 0.071
3.861ArgGly: 3.861 ± 0.095
0.811ArgHis: 0.811 ± 0.041
6.185ArgIle: 6.185 ± 0.113
4.749ArgLys: 4.749 ± 0.119
4.945ArgLeu: 4.945 ± 0.127
1.143ArgMet: 1.143 ± 0.057
2.346ArgAsn: 2.346 ± 0.074
1.627ArgPro: 1.627 ± 0.066
0.866ArgGln: 0.866 ± 0.044
3.916ArgArg: 3.916 ± 0.109
2.709ArgSer: 2.709 ± 0.081
2.01ArgThr: 2.01 ± 0.071
3.685ArgVal: 3.685 ± 0.108
0.521ArgTrp: 0.521 ± 0.038
2.289ArgTyr: 2.289 ± 0.083
0.0ArgXaa: 0.0 ± 0.0
Ser
2.652SerAla: 2.652 ± 0.087
0.251SerCys: 0.251 ± 0.021
2.449SerAsp: 2.449 ± 0.076
3.171SerGlu: 3.171 ± 0.091
2.324SerPhe: 2.324 ± 0.088
3.685SerGly: 3.685 ± 0.096
0.91SerHis: 0.91 ± 0.046
7.047SerIle: 7.047 ± 0.155
4.241SerLys: 4.241 ± 0.097
5.954SerLeu: 5.954 ± 0.126
1.598SerMet: 1.598 ± 0.065
2.276SerAsn: 2.276 ± 0.09
2.489SerPro: 2.489 ± 0.07
0.994SerGln: 0.994 ± 0.046
3.542SerArg: 3.542 ± 0.093
3.681SerSer: 3.681 ± 0.109
2.79SerThr: 2.79 ± 0.093
3.274SerVal: 3.274 ± 0.082
0.693SerTrp: 0.693 ± 0.044
2.562SerTyr: 2.562 ± 0.076
0.0SerXaa: 0.0 ± 0.0
Thr
2.759ThrAla: 2.759 ± 0.078
0.341ThrCys: 0.341 ± 0.032
1.752ThrAsp: 1.752 ± 0.059
1.836ThrGlu: 1.836 ± 0.07
1.321ThrPhe: 1.321 ± 0.057
3.89ThrGly: 3.89 ± 0.094
0.97ThrHis: 0.97 ± 0.044
5.715ThrIle: 5.715 ± 0.12
2.515ThrLys: 2.515 ± 0.091
4.738ThrLeu: 4.738 ± 0.124
1.216ThrMet: 1.216 ± 0.048
1.801ThrAsn: 1.801 ± 0.07
2.575ThrPro: 2.575 ± 0.081
0.82ThrGln: 0.82 ± 0.049
2.658ThrArg: 2.658 ± 0.079
3.08ThrSer: 3.08 ± 0.096
3.5ThrThr: 3.5 ± 0.297
3.21ThrVal: 3.21 ± 0.101
0.554ThrTrp: 0.554 ± 0.035
2.526ThrTyr: 2.526 ± 0.085
0.0ThrXaa: 0.0 ± 0.0
Val
4.404ValAla: 4.404 ± 0.112
0.365ValCys: 0.365 ± 0.027
3.775ValAsp: 3.775 ± 0.089
5.745ValGlu: 5.745 ± 0.133
3.527ValPhe: 3.527 ± 0.101
3.997ValGly: 3.997 ± 0.108
1.088ValHis: 1.088 ± 0.046
6.288ValIle: 6.288 ± 0.128
5.697ValLys: 5.697 ± 0.112
7.66ValLeu: 7.66 ± 0.145
1.502ValMet: 1.502 ± 0.054
2.711ValAsn: 2.711 ± 0.089
2.676ValPro: 2.676 ± 0.076
1.029ValGln: 1.029 ± 0.043
3.624ValArg: 3.624 ± 0.099
4.534ValSer: 4.534 ± 0.104
2.803ValThr: 2.803 ± 0.084
5.86ValVal: 5.86 ± 0.141
0.774ValTrp: 0.774 ± 0.047
3.582ValTyr: 3.582 ± 0.106
0.0ValXaa: 0.0 ± 0.0
Trp
0.585TrpAla: 0.585 ± 0.038
0.07TrpCys: 0.07 ± 0.013
0.624TrpAsp: 0.624 ± 0.036
0.708TrpGlu: 0.708 ± 0.044
0.611TrpPhe: 0.611 ± 0.037
0.767TrpGly: 0.767 ± 0.039
0.174TrpHis: 0.174 ± 0.021
1.251TrpIle: 1.251 ± 0.058
0.827TrpLys: 0.827 ± 0.04
1.183TrpLeu: 1.183 ± 0.052
0.303TrpMet: 0.303 ± 0.025
0.499TrpAsn: 0.499 ± 0.034
0.376TrpPro: 0.376 ± 0.029
0.204TrpGln: 0.204 ± 0.02
0.921TrpArg: 0.921 ± 0.056
0.734TrpSer: 0.734 ± 0.041
0.418TrpThr: 0.418 ± 0.036
0.761TrpVal: 0.761 ± 0.046
0.222TrpTrp: 0.222 ± 0.023
0.479TrpTyr: 0.479 ± 0.033
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.854TyrAla: 2.854 ± 0.076
0.396TyrCys: 0.396 ± 0.029
2.46TyrAsp: 2.46 ± 0.071
3.146TyrGlu: 3.146 ± 0.085
1.816TyrPhe: 1.816 ± 0.068
3.615TyrGly: 3.615 ± 0.096
0.838TyrHis: 0.838 ± 0.047
4.855TyrIle: 4.855 ± 0.115
3.3TyrLys: 3.3 ± 0.094
4.897TyrLeu: 4.897 ± 0.104
1.39TyrMet: 1.39 ± 0.051
2.372TyrAsn: 2.372 ± 0.076
2.203TyrPro: 2.203 ± 0.059
0.807TyrGln: 0.807 ± 0.046
3.166TyrArg: 3.166 ± 0.083
3.463TyrSer: 3.463 ± 0.105
2.487TyrThr: 2.487 ± 0.069
3.938TyrVal: 3.938 ± 0.102
0.64TyrTrp: 0.64 ± 0.041
2.803TyrTyr: 2.803 ± 0.085
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1570 proteins (454805 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski