Bacteroidales bacterium KA00344

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; unclassified Bacteroidales

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2869 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133YIG9|A0A133YIG9_9BACT Uncharacterized protein OS=Bacteroidales bacterium KA00344 OX=1497954 GN=HMPREF1870_01375 PE=4 SV=1
MM1 pKa = 7.65TYY3 pKa = 9.77FCKK6 pKa = 9.59SHH8 pKa = 6.52EE9 pKa = 4.24YY10 pKa = 10.49VRR12 pKa = 11.84VEE14 pKa = 4.41GEE16 pKa = 3.59FGYY19 pKa = 10.32IGITDD24 pKa = 3.95YY25 pKa = 11.29AQKK28 pKa = 11.21ALGNIVYY35 pKa = 10.47VDD37 pKa = 3.88MPDD40 pKa = 3.24VDD42 pKa = 5.32DD43 pKa = 4.74EE44 pKa = 4.68VTAGEE49 pKa = 4.33DD50 pKa = 3.39FGAVEE55 pKa = 4.41SVKK58 pKa = 10.62AASDD62 pKa = 3.47LMSPVSGVVVEE73 pKa = 4.31VNEE76 pKa = 4.1ALEE79 pKa = 4.93DD80 pKa = 3.9EE81 pKa = 5.08PEE83 pKa = 6.02LINQDD88 pKa = 4.53ANANWIIKK96 pKa = 9.93VKK98 pKa = 10.76LSDD101 pKa = 3.77VSEE104 pKa = 4.95LDD106 pKa = 3.7DD107 pKa = 5.87LMDD110 pKa = 4.02EE111 pKa = 4.31EE112 pKa = 6.48AYY114 pKa = 10.48LASVEE119 pKa = 4.12

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133Y580|A0A133Y580_9BACT Alanine--tRNA ligase OS=Bacteroidales bacterium KA00344 OX=1497954 GN=alaS PE=3 SV=1
MM1 pKa = 7.65LIVLIEE7 pKa = 3.72AVIRR11 pKa = 11.84KK12 pKa = 9.19RR13 pKa = 11.84ADD15 pKa = 3.1SVLKK19 pKa = 10.79VLAPASKK26 pKa = 10.72VKK28 pKa = 9.9ATTKK32 pKa = 8.74TAHH35 pKa = 6.98HH36 pKa = 6.43ITTIISHH43 pKa = 6.33VATASAKK50 pKa = 10.03AVTVLVTTIRR60 pKa = 11.84RR61 pKa = 11.84AVISLVRR68 pKa = 11.84VAIVPVITTIIKK80 pKa = 9.65RR81 pKa = 11.84AAISLVRR88 pKa = 11.84VVIVPVITTIIKK100 pKa = 9.65RR101 pKa = 11.84AAISLVRR108 pKa = 11.84VVIVPAIITIIRR120 pKa = 11.84RR121 pKa = 11.84ATISLVRR128 pKa = 11.84VVIAPVIITIIRR140 pKa = 11.84RR141 pKa = 11.84VAINLVRR148 pKa = 11.84AVIVPVIITIIRR160 pKa = 11.84RR161 pKa = 11.84AVINRR166 pKa = 11.84VATDD170 pKa = 3.07VRR172 pKa = 11.84VVINRR177 pKa = 11.84AVTVGRR183 pKa = 11.84TMIPMPNITT192 pKa = 4.17

Molecular weight: 20.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2869

0

2869

880993

35

2545

307.1

34.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.102 ± 0.047

1.274 ± 0.019

5.746 ± 0.034

5.959 ± 0.046

4.511 ± 0.028

6.764 ± 0.044

2.112 ± 0.021

6.714 ± 0.045

6.682 ± 0.038

8.755 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.003 ± 0.024

5.246 ± 0.046

3.64 ± 0.023

3.659 ± 0.025

4.967 ± 0.03

6.032 ± 0.041

5.714 ± 0.037

6.527 ± 0.033

1.258 ± 0.019

4.334 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski