Streptococcus phage Javan272

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AXB0|A0A4D6AXB0_9CAUD Membrane protein OS=Streptococcus phage Javan272 OX=2548087 GN=Javan272_0031 PE=4 SV=1
MM1 pKa = 7.97EE2 pKa = 4.6YY3 pKa = 8.11TTEE6 pKa = 4.07LNGKK10 pKa = 8.7KK11 pKa = 10.11LIYY14 pKa = 10.32GPLVEE19 pKa = 5.0NSNFTAKK26 pKa = 9.37EE27 pKa = 3.44WSAIYY32 pKa = 10.82AEE34 pKa = 4.49TVKK37 pKa = 10.67QNQPEE42 pKa = 4.28VFGTKK47 pKa = 10.21KK48 pKa = 10.3NDD50 pKa = 3.12KK51 pKa = 10.7DD52 pKa = 4.12YY53 pKa = 11.3IDD55 pKa = 3.84VFGALISLEE64 pKa = 3.77EE65 pKa = 4.28RR66 pKa = 11.84YY67 pKa = 9.72EE68 pKa = 4.0VLLEE72 pKa = 3.91NLPQNSYY79 pKa = 10.97SKK81 pKa = 11.04AGTHH85 pKa = 5.96PMWVANAVEE94 pKa = 4.88DD95 pKa = 3.66NTLNRR100 pKa = 11.84EE101 pKa = 3.87ATTADD106 pKa = 2.99ISEE109 pKa = 4.35MIEE112 pKa = 3.81NCEE115 pKa = 4.0SLEE118 pKa = 3.95EE119 pKa = 4.01LVEE122 pKa = 4.01EE123 pKa = 4.05LTDD126 pKa = 3.74YY127 pKa = 11.5FEE129 pKa = 6.26LEE131 pKa = 4.24VEE133 pKa = 4.29

Molecular weight: 15.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AWL0|A0A4D6AWL0_9CAUD Uncharacterized protein OS=Streptococcus phage Javan272 OX=2548087 GN=Javan272_0012 PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 4.78EE3 pKa = 3.74VSPIKK8 pKa = 11.04DD9 pKa = 3.39NDD11 pKa = 4.55DD12 pKa = 3.23IQAMKK17 pKa = 10.62DD18 pKa = 3.6YY19 pKa = 10.67LRR21 pKa = 11.84EE22 pKa = 4.13WNEE25 pKa = 3.47MYY27 pKa = 11.37YY28 pKa = 9.99MLFITGLNTGLRR40 pKa = 11.84VGDD43 pKa = 3.65ILTLKK48 pKa = 10.66VKK50 pKa = 10.31DD51 pKa = 3.59VQGWHH56 pKa = 5.33IKK58 pKa = 9.99LRR60 pKa = 11.84EE61 pKa = 4.04RR62 pKa = 11.84KK63 pKa = 7.72TGKK66 pKa = 10.21QITRR70 pKa = 11.84RR71 pKa = 11.84MTKK74 pKa = 8.23EE75 pKa = 3.51LKK77 pKa = 10.21KK78 pKa = 10.38EE79 pKa = 3.51MRR81 pKa = 11.84RR82 pKa = 11.84YY83 pKa = 10.15VEE85 pKa = 5.19DD86 pKa = 3.53KK87 pKa = 10.36PFHH90 pKa = 6.39HH91 pKa = 7.05FLFKK95 pKa = 10.73SRR97 pKa = 11.84QGKK100 pKa = 8.92NKK102 pKa = 9.84PITRR106 pKa = 11.84EE107 pKa = 3.16RR108 pKa = 11.84AYY110 pKa = 10.73QIIHH114 pKa = 6.19EE115 pKa = 4.43AAEE118 pKa = 3.91EE119 pKa = 3.97LGIDD123 pKa = 3.49NVGTHH128 pKa = 5.72TMRR131 pKa = 11.84KK132 pKa = 6.62TFGYY136 pKa = 10.16KK137 pKa = 10.08YY138 pKa = 9.91YY139 pKa = 11.16NKK141 pKa = 9.47TKK143 pKa = 10.72DD144 pKa = 3.11VGTLQKK150 pKa = 9.79MFNHH154 pKa = 6.53SSPAITLRR162 pKa = 11.84YY163 pKa = 9.45IGIEE167 pKa = 3.91QAEE170 pKa = 4.45LDD172 pKa = 3.58DD173 pKa = 4.2ALRR176 pKa = 11.84NFVII180 pKa = 4.81

Molecular weight: 21.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

9170

40

934

195.1

22.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.532 ± 0.53

0.534 ± 0.107

6.314 ± 0.344

7.721 ± 0.511

4.351 ± 0.347

5.758 ± 0.4

1.527 ± 0.196

7.426 ± 0.378

8.135 ± 0.395

8.288 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.661 ± 0.177

6.02 ± 0.295

2.65 ± 0.181

3.806 ± 0.276

3.991 ± 0.336

6.423 ± 0.408

6.281 ± 0.368

6.27 ± 0.262

1.167 ± 0.138

4.144 ± 0.462
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski