Bifidobacterium phage BadAztec4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Badaztecvirus; unclassified Badaztecvirus

Average proteome isoelectric point is 5.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6Y018|A0A6G6Y018_9CAUD Uncharacterized protein OS=Bifidobacterium phage BadAztec4 OX=2713245 GN=BAAZ0010004c01_00014 PE=4 SV=1
MM1 pKa = 6.56ITKK4 pKa = 9.92VNYY7 pKa = 9.03EE8 pKa = 3.96FQNEE12 pKa = 3.91VLRR15 pKa = 11.84YY16 pKa = 9.92VNLIVDD22 pKa = 4.51KK23 pKa = 11.0YY24 pKa = 10.91SGRR27 pKa = 11.84ADD29 pKa = 2.99IEE31 pKa = 4.24FNEE34 pKa = 4.79TYY36 pKa = 11.0LEE38 pKa = 4.12ITLSCGDD45 pKa = 3.36ASLWINVYY53 pKa = 10.21FSGRR57 pKa = 11.84MTSVFYY63 pKa = 10.22PKK65 pKa = 10.46NGNEE69 pKa = 4.11YY70 pKa = 11.03VEE72 pKa = 4.24GTKK75 pKa = 10.76DD76 pKa = 3.22GFHH79 pKa = 7.29EE80 pKa = 4.3INYY83 pKa = 9.16VDD85 pKa = 4.58LRR87 pKa = 11.84RR88 pKa = 11.84MYY90 pKa = 11.36DD91 pKa = 3.13DD92 pKa = 5.43FIRR95 pKa = 11.84FFSEE99 pKa = 3.52YY100 pKa = 9.51SYY102 pKa = 11.18SIEE105 pKa = 3.97PSFF108 pKa = 4.67

Molecular weight: 12.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6Y031|A0A6G6Y031_9CAUD DNA-directed DNA polymerase OS=Bifidobacterium phage BadAztec4 OX=2713245 GN=BAAZ0010004c01_00021 PE=4 SV=1
MM1 pKa = 7.18ARR3 pKa = 11.84KK4 pKa = 9.43RR5 pKa = 11.84KK6 pKa = 9.33ISKK9 pKa = 8.15SQRR12 pKa = 11.84KK13 pKa = 7.9AAQRR17 pKa = 11.84KK18 pKa = 8.63RR19 pKa = 11.84NAQVRR24 pKa = 11.84SAQVARR30 pKa = 11.84GVSKK34 pKa = 10.85RR35 pKa = 11.84IDD37 pKa = 3.73TINPTKK43 pKa = 10.52ISVGTLNKK51 pKa = 10.28LSRR54 pKa = 11.84PQLEE58 pKa = 3.69RR59 pKa = 11.84LAQNVGNEE67 pKa = 4.07VYY69 pKa = 10.16DD70 pKa = 3.8AKK72 pKa = 11.32NSVEE76 pKa = 4.02KK77 pKa = 10.69YY78 pKa = 10.22LRR80 pKa = 11.84SKK82 pKa = 10.37PYY84 pKa = 8.88YY85 pKa = 7.66HH86 pKa = 7.0TPPVSVSKK94 pKa = 10.88RR95 pKa = 11.84EE96 pKa = 3.82RR97 pKa = 11.84EE98 pKa = 3.7FAARR102 pKa = 11.84KK103 pKa = 9.59IPTDD107 pKa = 3.51QQIAAAPPKK116 pKa = 9.7KK117 pKa = 10.34AKK119 pKa = 10.18LLRR122 pKa = 11.84QQKK125 pKa = 10.16RR126 pKa = 11.84KK127 pKa = 8.18ITAARR132 pKa = 11.84NKK134 pKa = 10.25LEE136 pKa = 3.68EE137 pKa = 4.16WKK139 pKa = 10.49QYY141 pKa = 8.98NAQVVAGRR149 pKa = 11.84NAKK152 pKa = 8.45EE153 pKa = 3.82EE154 pKa = 4.05RR155 pKa = 11.84ADD157 pKa = 3.69ALNGTGGKK165 pKa = 8.18QSRR168 pKa = 11.84TRR170 pKa = 11.84DD171 pKa = 3.04AGKK174 pKa = 10.48RR175 pKa = 11.84STSDD179 pKa = 2.42IDD181 pKa = 3.56RR182 pKa = 11.84LDD184 pKa = 3.48RR185 pKa = 11.84YY186 pKa = 10.99RR187 pKa = 11.84NVLKK191 pKa = 10.81NPQIVSKK198 pKa = 9.77LTDD201 pKa = 3.42DD202 pKa = 3.5EE203 pKa = 4.22LRR205 pKa = 11.84RR206 pKa = 11.84EE207 pKa = 3.62IRR209 pKa = 11.84ARR211 pKa = 11.84SRR213 pKa = 11.84DD214 pKa = 3.61LKK216 pKa = 10.82SAEE219 pKa = 3.78NLIDD223 pKa = 3.78KK224 pKa = 10.67GVGRR228 pKa = 11.84INARR232 pKa = 11.84DD233 pKa = 3.25NAYY236 pKa = 10.31VEE238 pKa = 4.44KK239 pKa = 10.43RR240 pKa = 11.84LKK242 pKa = 10.91AGFGQSALEE251 pKa = 4.07SWRR254 pKa = 11.84KK255 pKa = 9.22LSRR258 pKa = 11.84SEE260 pKa = 4.09RR261 pKa = 11.84YY262 pKa = 9.85SLINQTSLLNRR273 pKa = 11.84IEE275 pKa = 4.6FYY277 pKa = 10.88SVYY280 pKa = 10.65NEE282 pKa = 3.69EE283 pKa = 4.03THH285 pKa = 7.47AYY287 pKa = 8.91EE288 pKa = 4.39PWDD291 pKa = 4.11HH292 pKa = 7.42DD293 pKa = 3.47RR294 pKa = 11.84QDD296 pKa = 3.21QVKK299 pKa = 9.85RR300 pKa = 11.84EE301 pKa = 4.03RR302 pKa = 11.84SDD304 pKa = 2.67IMAFLRR310 pKa = 11.84NAKK313 pKa = 10.02NISQATLEE321 pKa = 4.14

Molecular weight: 37.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

6211

55

699

258.8

29.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.712 ± 0.506

1.014 ± 0.194

7.648 ± 0.462

4.878 ± 0.324

3.977 ± 0.384

6.666 ± 0.502

1.691 ± 0.183

6.086 ± 0.279

5.329 ± 0.493

6.988 ± 0.43

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.93 ± 0.245

6.424 ± 0.404

3.784 ± 0.257

4.218 ± 0.43

5.49 ± 0.542

6.972 ± 0.545

6.295 ± 0.398

5.844 ± 0.386

1.626 ± 0.172

4.395 ± 0.287
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski