Qinghai Lake virophage

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Maveriviricetes; Priklausovirales; Lavidaviridae; unclassified Lavidaviridae

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R5K6B7|A0A0R5K6B7_9VIRU Uncharacterized protein OS=Qinghai Lake virophage OX=1516115 GN=QLV_13 PE=3 SV=1
MM1 pKa = 6.98SQQVSKK7 pKa = 10.3ATTPDD12 pKa = 2.87MVYY15 pKa = 11.06YY16 pKa = 10.46DD17 pKa = 3.12IVVTNFQSITTKK29 pKa = 10.64APKK32 pKa = 10.22LRR34 pKa = 11.84FNEE37 pKa = 4.09SRR39 pKa = 11.84TNPIIDD45 pKa = 3.65KK46 pKa = 10.47TEE48 pKa = 3.99DD49 pKa = 3.61YY50 pKa = 11.1YY51 pKa = 11.32MSIVRR56 pKa = 11.84FSLDD60 pKa = 3.13TYY62 pKa = 10.54NLPNIICEE70 pKa = 4.08IQPNQADD77 pKa = 4.25PNLSIYY83 pKa = 10.86SVTLQALLPDD93 pKa = 4.03GVGGFTTTEE102 pKa = 3.8VQTFLNWIPQNRR114 pKa = 11.84DD115 pKa = 2.47IPTPLAPSLTTTGFQMTNTEE135 pKa = 4.15YY136 pKa = 10.29YY137 pKa = 9.7YY138 pKa = 10.53CYY140 pKa = 10.01QFQYY144 pKa = 10.99FLALMNQAFQTCLVDD159 pKa = 6.08LITATGGGASPINTAQQPIILWDD182 pKa = 3.76FTSNSAILNAPSNFYY197 pKa = 10.73EE198 pKa = 4.25VTIDD202 pKa = 3.44PTVNPIINIFFNKK215 pKa = 9.72PLFALFNSFLNIDD228 pKa = 4.14FGVSITNGKK237 pKa = 7.76NHH239 pKa = 6.57QIVIDD244 pKa = 4.32SYY246 pKa = 11.66LGTQTIQFPYY256 pKa = 10.66GLDD259 pKa = 3.4PEE261 pKa = 5.41LEE263 pKa = 4.32YY264 pKa = 10.51TQTFQEE270 pKa = 4.99FSTIDD275 pKa = 2.63TWTPVSSIVFTSNTIPIVSNQLSAPLIFNDD305 pKa = 4.06GQIVVGDD312 pKa = 3.78GNNCNFAQIITDD324 pKa = 4.3LEE326 pKa = 4.51TNQQVYY332 pKa = 9.71KK333 pKa = 10.13PQLLYY338 pKa = 11.19VPTAEE343 pKa = 4.22YY344 pKa = 10.68RR345 pKa = 11.84RR346 pKa = 11.84IDD348 pKa = 3.48LTGNRR353 pKa = 11.84PLTNIDD359 pKa = 3.31IEE361 pKa = 4.82VYY363 pKa = 9.64WRR365 pKa = 11.84DD366 pKa = 3.24KK367 pKa = 11.4LGGLNQLNLEE377 pKa = 4.45SGGSATIKK385 pKa = 10.56FLFEE389 pKa = 4.49KK390 pKa = 10.31KK391 pKa = 10.1DD392 pKa = 3.27KK393 pKa = 11.34LGIKK397 pKa = 10.26GYY399 pKa = 10.86

Molecular weight: 44.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R5K4Q4|A0A0R5K4Q4_9VIRU Uncharacterized protein OS=Qinghai Lake virophage OX=1516115 GN=QLV_11 PE=4 SV=1
MM1 pKa = 7.6IEE3 pKa = 4.65AYY5 pKa = 9.86DD6 pKa = 3.71IKK8 pKa = 10.7KK9 pKa = 8.78TDD11 pKa = 4.12PLWKK15 pKa = 9.8YY16 pKa = 10.39SNPITAQNKK25 pKa = 7.18TYY27 pKa = 10.76SYY29 pKa = 10.17LGSDD33 pKa = 2.91ATLYY37 pKa = 10.92KK38 pKa = 9.55STRR41 pKa = 11.84EE42 pKa = 3.88GKK44 pKa = 10.37KK45 pKa = 10.86YY46 pKa = 9.4MVKK49 pKa = 10.28NPQGRR54 pKa = 11.84LIHH57 pKa = 6.5FGQLGYY63 pKa = 11.34EE64 pKa = 4.56DD65 pKa = 4.42FNKK68 pKa = 10.32HH69 pKa = 4.87KK70 pKa = 10.81SRR72 pKa = 11.84LRR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84AYY78 pKa = 10.68LNRR81 pKa = 11.84ATKK84 pKa = 8.79ITGDD88 pKa = 3.43WKK90 pKa = 10.54KK91 pKa = 11.12DD92 pKa = 3.09KK93 pKa = 10.96YY94 pKa = 11.28SPNNLSINILWW105 pKa = 3.71

Molecular weight: 12.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

6731

72

839

269.2

30.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.873 ± 0.814

1.307 ± 0.307

6.091 ± 0.435

6.522 ± 0.811

4.338 ± 0.378

6.983 ± 1.032

1.337 ± 0.261

7.488 ± 0.341

8.884 ± 1.264

8.439 ± 0.456

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.241

6.864 ± 0.379

3.863 ± 0.369

3.684 ± 0.55

4.487 ± 0.532

6.091 ± 0.888

5.854 ± 0.532

4.962 ± 0.359

0.906 ± 0.183

4.754 ± 0.444
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski