Candidatus Jidaibacter acanthamoeba

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Candidatus Midichloriaceae; Candidatus Jidaibacter

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C1R1F2|A0A0C1R1F2_9RICK Putative membrane protein insertion efficiency factor OS=Candidatus Jidaibacter acanthamoeba OX=86105 GN=NF27_BK00410 PE=3 SV=1
MM1 pKa = 7.4FNIGYY6 pKa = 10.52YY7 pKa = 10.88NMNSFKK13 pKa = 11.12NPFYY17 pKa = 10.82LSSLNGRR24 pKa = 11.84NGFAINGTTEE34 pKa = 3.83SMMEE38 pKa = 3.94SGFSVSRR45 pKa = 11.84AGDD48 pKa = 3.4INGDD52 pKa = 4.21GIADD56 pKa = 5.08LIIGAPGARR65 pKa = 11.84LKK67 pKa = 10.43MGASYY72 pKa = 11.32VVFGSQSPFSANTSLSDD89 pKa = 3.74LNGNNGFAIYY99 pKa = 10.46GRR101 pKa = 11.84TSLDD105 pKa = 3.38SLGVSVNGAGDD116 pKa = 3.52INGDD120 pKa = 4.06SIADD124 pKa = 4.69LIVSGAQTGAGYY136 pKa = 10.6VIFGSRR142 pKa = 11.84SPFPGNISLSDD153 pKa = 3.42INGKK157 pKa = 9.62NGFTISGLGNNYY169 pKa = 8.27WSKK172 pKa = 10.81PPVSGAGDD180 pKa = 3.4INADD184 pKa = 4.44GIADD188 pKa = 4.27MIIGDD193 pKa = 4.06SDD195 pKa = 3.52AGASYY200 pKa = 9.84TIFGSKK206 pKa = 10.52YY207 pKa = 9.37PFPANINVSEE217 pKa = 4.29LNGKK221 pKa = 9.24NGFTIYY227 pKa = 10.65GITSFDD233 pKa = 3.4SLGYY237 pKa = 9.3SVSGAGDD244 pKa = 3.29INADD248 pKa = 4.24GIADD252 pKa = 3.6IVIGAFTAGSNVGASYY268 pKa = 11.41VVFGSQSPFPTSINVYY284 pKa = 10.08SLNGKK289 pKa = 9.33NGFAINGIASLDD301 pKa = 3.64ALGSSVSGAGDD312 pKa = 3.32INGDD316 pKa = 4.23GIADD320 pKa = 5.08LIIGAPGASLKK331 pKa = 10.91AGASYY336 pKa = 10.97VIFGSQSTFPASINVSDD353 pKa = 4.88LNGKK357 pKa = 9.32NGFAINGITNPDD369 pKa = 3.42ALGSSVSGAGDD380 pKa = 3.32INGDD384 pKa = 4.15GIADD388 pKa = 4.72FIIGAPGAGSNVGASYY404 pKa = 11.41VVFGSQSFLPASINVSDD421 pKa = 4.05LNGRR425 pKa = 11.84NGFAIYY431 pKa = 9.64GAKK434 pKa = 10.09AYY436 pKa = 8.35EE437 pKa = 3.84NSGYY441 pKa = 10.16SVSGAGDD448 pKa = 3.46INDD451 pKa = 5.08DD452 pKa = 4.6GIADD456 pKa = 5.17LIIGAPEE463 pKa = 4.41AGSGTSYY470 pKa = 11.33VVFGMKK476 pKa = 8.82STTASLQISGSSYY489 pKa = 10.76SDD491 pKa = 3.07SLIYY495 pKa = 10.03EE496 pKa = 4.56DD497 pKa = 5.98KK498 pKa = 10.42EE499 pKa = 4.57TFLMDD504 pKa = 3.31TDD506 pKa = 4.14LTLKK510 pKa = 10.82LEE512 pKa = 4.31SEE514 pKa = 4.46II515 pKa = 4.81

Molecular weight: 51.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C1QFZ1|A0A0C1QFZ1_9RICK Uncharacterized protein OS=Candidatus Jidaibacter acanthamoeba OX=86105 GN=NF27_HQ00070 PE=4 SV=1
MM1 pKa = 7.89SYY3 pKa = 10.46RR4 pKa = 11.84IPKK7 pKa = 8.73TLDD10 pKa = 2.88NPIRR14 pKa = 11.84CVGIPIDD21 pKa = 3.62TLMVFMVIWSALFLFDD37 pKa = 3.7QPIWGMVAGVIGANVFSRR55 pKa = 11.84YY56 pKa = 8.32RR57 pKa = 11.84SRR59 pKa = 11.84SITRR63 pKa = 11.84RR64 pKa = 11.84LVRR67 pKa = 11.84FIYY70 pKa = 9.47WYY72 pKa = 10.27LPCEE76 pKa = 4.1VNFIRR81 pKa = 11.84GVQGHH86 pKa = 4.57QRR88 pKa = 11.84KK89 pKa = 8.33MNMEE93 pKa = 4.32LKK95 pKa = 9.83KK96 pKa = 10.1WKK98 pKa = 10.27

Molecular weight: 11.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2204

0

2204

678764

29

3780

308.0

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.489 ± 0.05

1.016 ± 0.016

4.84 ± 0.04

7.327 ± 0.071

4.443 ± 0.049

5.718 ± 0.055

1.869 ± 0.021

9.199 ± 0.056

8.592 ± 0.056

10.108 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.125 ± 0.024

6.332 ± 0.058

3.086 ± 0.033

3.238 ± 0.033

3.782 ± 0.047

6.871 ± 0.047

4.738 ± 0.041

5.392 ± 0.047

0.816 ± 0.016

4.014 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski