Amino acid dipepetide frequency for Massilia sp. ONC3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.693AlaAla: 16.693 ± 0.177
1.245AlaCys: 1.245 ± 0.028
5.987AlaAsp: 5.987 ± 0.066
6.08AlaGlu: 6.08 ± 0.077
3.947AlaPhe: 3.947 ± 0.05
10.266AlaGly: 10.266 ± 0.095
2.618AlaHis: 2.618 ± 0.047
5.562AlaIle: 5.562 ± 0.066
4.045AlaLys: 4.045 ± 0.068
13.64AlaLeu: 13.64 ± 0.128
3.163AlaMet: 3.163 ± 0.043
3.546AlaAsn: 3.546 ± 0.049
6.121AlaPro: 6.121 ± 0.075
5.393AlaGln: 5.393 ± 0.075
8.612AlaArg: 8.612 ± 0.091
6.73AlaSer: 6.73 ± 0.063
5.949AlaThr: 5.949 ± 0.073
8.176AlaVal: 8.176 ± 0.079
1.693AlaTrp: 1.693 ± 0.036
2.664AlaTyr: 2.664 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
1.288CysAla: 1.288 ± 0.03
0.124CysCys: 0.124 ± 0.01
0.517CysAsp: 0.517 ± 0.019
0.479CysGlu: 0.479 ± 0.018
0.294CysPhe: 0.294 ± 0.014
0.946CysGly: 0.946 ± 0.025
0.254CysHis: 0.254 ± 0.012
0.396CysIle: 0.396 ± 0.016
0.246CysLys: 0.246 ± 0.013
0.806CysLeu: 0.806 ± 0.026
0.197CysMet: 0.197 ± 0.012
0.253CysAsn: 0.253 ± 0.016
0.467CysPro: 0.467 ± 0.021
0.289CysGln: 0.289 ± 0.014
0.573CysArg: 0.573 ± 0.02
0.533CysSer: 0.533 ± 0.016
0.468CysThr: 0.468 ± 0.019
0.67CysVal: 0.67 ± 0.024
0.13CysTrp: 0.13 ± 0.01
0.244CysTyr: 0.244 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.507AspAla: 6.507 ± 0.067
0.493AspCys: 0.493 ± 0.019
2.682AspAsp: 2.682 ± 0.048
3.319AspGlu: 3.319 ± 0.05
2.069AspPhe: 2.069 ± 0.039
4.718AspGly: 4.718 ± 0.06
1.051AspHis: 1.051 ± 0.029
2.579AspIle: 2.579 ± 0.043
2.035AspLys: 2.035 ± 0.043
5.298AspLeu: 5.298 ± 0.063
1.258AspMet: 1.258 ± 0.029
1.479AspAsn: 1.479 ± 0.034
3.188AspPro: 3.188 ± 0.051
1.971AspGln: 1.971 ± 0.037
3.094AspArg: 3.094 ± 0.05
2.509AspSer: 2.509 ± 0.04
2.576AspThr: 2.576 ± 0.042
3.907AspVal: 3.907 ± 0.046
0.925AspTrp: 0.925 ± 0.025
1.597AspTyr: 1.597 ± 0.032
0.0AspXaa: 0.0 ± 0.0
Glu
6.12GluAla: 6.12 ± 0.078
0.416GluCys: 0.416 ± 0.016
2.229GluAsp: 2.229 ± 0.037
2.764GluGlu: 2.764 ± 0.049
1.865GluPhe: 1.865 ± 0.035
3.55GluGly: 3.55 ± 0.047
1.442GluHis: 1.442 ± 0.03
2.833GluIle: 2.833 ± 0.041
2.065GluLys: 2.065 ± 0.042
6.16GluLeu: 6.16 ± 0.065
1.43GluMet: 1.43 ± 0.031
1.557GluAsn: 1.557 ± 0.033
2.584GluPro: 2.584 ± 0.045
3.033GluGln: 3.033 ± 0.049
4.76GluArg: 4.76 ± 0.067
2.554GluSer: 2.554 ± 0.042
2.533GluThr: 2.533 ± 0.039
3.872GluVal: 3.872 ± 0.056
0.764GluTrp: 0.764 ± 0.024
1.293GluTyr: 1.293 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
4.272PheAla: 4.272 ± 0.054
0.426PheCys: 0.426 ± 0.019
2.606PheAsp: 2.606 ± 0.04
2.091PheGlu: 2.091 ± 0.035
1.367PhePhe: 1.367 ± 0.037
3.507PheGly: 3.507 ± 0.058
0.752PheHis: 0.752 ± 0.024
1.587PheIle: 1.587 ± 0.028
1.283PheLys: 1.283 ± 0.03
3.039PheLeu: 3.039 ± 0.043
0.802PheMet: 0.802 ± 0.022
1.292PheAsn: 1.292 ± 0.033
1.566PhePro: 1.566 ± 0.034
1.08PheGln: 1.08 ± 0.027
2.038PheArg: 2.038 ± 0.032
2.231PheSer: 2.231 ± 0.04
2.019PheThr: 2.019 ± 0.036
2.801PheVal: 2.801 ± 0.044
0.508PheTrp: 0.508 ± 0.021
1.012PheTyr: 1.012 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
9.026GlyAla: 9.026 ± 0.086
0.85GlyCys: 0.85 ± 0.026
3.984GlyAsp: 3.984 ± 0.056
4.492GlyGlu: 4.492 ± 0.052
3.198GlyPhe: 3.198 ± 0.046
6.791GlyGly: 6.791 ± 0.088
1.864GlyHis: 1.864 ± 0.037
4.132GlyIle: 4.132 ± 0.051
3.807GlyLys: 3.807 ± 0.053
7.893GlyLeu: 7.893 ± 0.077
2.392GlyMet: 2.392 ± 0.038
2.571GlyAsn: 2.571 ± 0.048
3.094GlyPro: 3.094 ± 0.049
3.3GlyGln: 3.3 ± 0.045
5.126GlyArg: 5.126 ± 0.06
4.691GlySer: 4.691 ± 0.056
4.593GlyThr: 4.593 ± 0.063
6.118GlyVal: 6.118 ± 0.057
1.373GlyTrp: 1.373 ± 0.031
2.443GlyTyr: 2.443 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.686HisAla: 2.686 ± 0.045
0.262HisCys: 0.262 ± 0.014
1.228HisAsp: 1.228 ± 0.025
1.206HisGlu: 1.206 ± 0.028
0.953HisPhe: 0.953 ± 0.022
2.066HisGly: 2.066 ± 0.034
0.599HisHis: 0.599 ± 0.023
0.985HisIle: 0.985 ± 0.026
0.669HisLys: 0.669 ± 0.021
2.196HisLeu: 2.196 ± 0.042
0.54HisMet: 0.54 ± 0.019
0.631HisAsn: 0.631 ± 0.021
1.427HisPro: 1.427 ± 0.033
0.746HisGln: 0.746 ± 0.021
1.296HisArg: 1.296 ± 0.027
1.055HisSer: 1.055 ± 0.026
1.042HisThr: 1.042 ± 0.024
1.528HisVal: 1.528 ± 0.031
0.354HisTrp: 0.354 ± 0.016
0.678HisTyr: 0.678 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
6.242IleAla: 6.242 ± 0.068
0.428IleCys: 0.428 ± 0.018
3.403IleAsp: 3.403 ± 0.049
3.342IleGlu: 3.342 ± 0.051
1.334IlePhe: 1.334 ± 0.031
4.488IleGly: 4.488 ± 0.057
0.855IleHis: 0.855 ± 0.024
1.866IleIle: 1.866 ± 0.036
1.785IleLys: 1.785 ± 0.032
3.713IleLeu: 3.713 ± 0.057
0.887IleMet: 0.887 ± 0.024
1.659IleAsn: 1.659 ± 0.033
2.136IlePro: 2.136 ± 0.041
1.2IleGln: 1.2 ± 0.028
2.756IleArg: 2.756 ± 0.041
2.624IleSer: 2.624 ± 0.044
2.58IleThr: 2.58 ± 0.042
3.748IleVal: 3.748 ± 0.055
0.488IleTrp: 0.488 ± 0.018
1.092IleTyr: 1.092 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
4.126LysAla: 4.126 ± 0.067
0.215LysCys: 0.215 ± 0.012
1.82LysAsp: 1.82 ± 0.039
1.812LysGlu: 1.812 ± 0.04
1.1LysPhe: 1.1 ± 0.03
2.496LysGly: 2.496 ± 0.045
0.744LysHis: 0.744 ± 0.02
1.785LysIle: 1.785 ± 0.041
1.557LysLys: 1.557 ± 0.036
3.925LysLeu: 3.925 ± 0.055
0.998LysMet: 0.998 ± 0.028
1.253LysAsn: 1.253 ± 0.033
2.217LysPro: 2.217 ± 0.04
1.46LysGln: 1.46 ± 0.031
2.393LysArg: 2.393 ± 0.039
1.897LysSer: 1.897 ± 0.033
2.063LysThr: 2.063 ± 0.038
2.665LysVal: 2.665 ± 0.045
0.426LysTrp: 0.426 ± 0.017
0.85LysTyr: 0.85 ± 0.027
0.0LysXaa: 0.0 ± 0.0
Leu
14.686LeuAla: 14.686 ± 0.135
0.972LeuCys: 0.972 ± 0.022
5.892LeuAsp: 5.892 ± 0.066
5.366LeuGlu: 5.366 ± 0.072
3.7LeuPhe: 3.7 ± 0.053
7.974LeuGly: 7.974 ± 0.082
2.282LeuHis: 2.282 ± 0.041
4.19LeuIle: 4.19 ± 0.059
3.688LeuLys: 3.688 ± 0.048
10.648LeuLeu: 10.648 ± 0.118
2.311LeuMet: 2.311 ± 0.043
2.935LeuAsn: 2.935 ± 0.051
5.878LeuPro: 5.878 ± 0.066
3.648LeuGln: 3.648 ± 0.047
7.259LeuArg: 7.259 ± 0.08
5.853LeuSer: 5.853 ± 0.073
5.29LeuThr: 5.29 ± 0.052
7.743LeuVal: 7.743 ± 0.08
1.167LeuTrp: 1.167 ± 0.027
2.366LeuTyr: 2.366 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
2.884MetAla: 2.884 ± 0.048
0.168MetCys: 0.168 ± 0.009
1.091MetAsp: 1.091 ± 0.03
1.082MetGlu: 1.082 ± 0.025
0.841MetPhe: 0.841 ± 0.022
1.669MetGly: 1.669 ± 0.038
0.574MetHis: 0.574 ± 0.02
1.135MetIle: 1.135 ± 0.032
1.165MetLys: 1.165 ± 0.031
2.772MetLeu: 2.772 ± 0.044
0.632MetMet: 0.632 ± 0.022
0.941MetAsn: 0.941 ± 0.025
1.474MetPro: 1.474 ± 0.032
1.104MetGln: 1.104 ± 0.026
1.795MetArg: 1.795 ± 0.03
1.574MetSer: 1.574 ± 0.031
1.494MetThr: 1.494 ± 0.03
1.694MetVal: 1.694 ± 0.033
0.235MetTrp: 0.235 ± 0.013
0.477MetTyr: 0.477 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.491AsnAla: 3.491 ± 0.052
0.338AsnCys: 0.338 ± 0.019
1.579AsnAsp: 1.579 ± 0.03
1.5AsnGlu: 1.5 ± 0.033
1.149AsnPhe: 1.149 ± 0.029
2.837AsnGly: 2.837 ± 0.06
0.613AsnHis: 0.613 ± 0.02
1.519AsnIle: 1.519 ± 0.035
1.073AsnLys: 1.073 ± 0.024
3.119AsnLeu: 3.119 ± 0.05
0.7AsnMet: 0.7 ± 0.022
1.103AsnAsn: 1.103 ± 0.034
2.014AsnPro: 2.014 ± 0.04
1.135AsnGln: 1.135 ± 0.03
1.967AsnArg: 1.967 ± 0.037
1.565AsnSer: 1.565 ± 0.034
1.585AsnThr: 1.585 ± 0.038
2.382AsnVal: 2.382 ± 0.039
0.508AsnTrp: 0.508 ± 0.019
0.921AsnTyr: 0.921 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
6.779ProAla: 6.779 ± 0.09
0.387ProCys: 0.387 ± 0.016
3.362ProAsp: 3.362 ± 0.046
3.216ProGlu: 3.216 ± 0.054
1.815ProPhe: 1.815 ± 0.039
4.758ProGly: 4.758 ± 0.06
1.1ProHis: 1.1 ± 0.026
2.046ProIle: 2.046 ± 0.035
1.646ProLys: 1.646 ± 0.034
5.062ProLeu: 5.062 ± 0.057
1.173ProMet: 1.173 ± 0.024
1.664ProAsn: 1.664 ± 0.034
2.664ProPro: 2.664 ± 0.06
2.081ProGln: 2.081 ± 0.036
2.791ProArg: 2.791 ± 0.045
2.832ProSer: 2.832 ± 0.052
2.403ProThr: 2.403 ± 0.038
4.262ProVal: 4.262 ± 0.054
0.623ProTrp: 0.623 ± 0.017
1.268ProTyr: 1.268 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
5.256GlnAla: 5.256 ± 0.071
0.284GlnCys: 0.284 ± 0.014
1.707GlnAsp: 1.707 ± 0.034
1.852GlnGlu: 1.852 ± 0.039
1.482GlnPhe: 1.482 ± 0.025
2.76GlnGly: 2.76 ± 0.045
0.946GlnHis: 0.946 ± 0.024
1.838GlnIle: 1.838 ± 0.037
1.202GlnLys: 1.202 ± 0.034
4.327GlnLeu: 4.327 ± 0.06
1.062GlnMet: 1.062 ± 0.027
1.026GlnAsn: 1.026 ± 0.027
2.185GlnPro: 2.185 ± 0.039
2.091GlnGln: 2.091 ± 0.047
2.981GlnArg: 2.981 ± 0.052
1.99GlnSer: 1.99 ± 0.038
1.841GlnThr: 1.841 ± 0.037
3.218GlnVal: 3.218 ± 0.045
0.506GlnTrp: 0.506 ± 0.018
0.958GlnTyr: 0.958 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
7.508ArgAla: 7.508 ± 0.086
0.527ArgCys: 0.527 ± 0.016
3.643ArgAsp: 3.643 ± 0.054
4.037ArgGlu: 4.037 ± 0.055
2.785ArgPhe: 2.785 ± 0.045
4.368ArgGly: 4.368 ± 0.058
1.747ArgHis: 1.747 ± 0.032
3.611ArgIle: 3.611 ± 0.048
2.268ArgLys: 2.268 ± 0.04
7.524ArgLeu: 7.524 ± 0.081
1.871ArgMet: 1.871 ± 0.039
2.102ArgAsn: 2.102 ± 0.038
3.069ArgPro: 3.069 ± 0.042
2.959ArgGln: 2.959 ± 0.045
4.818ArgArg: 4.818 ± 0.068
3.355ArgSer: 3.355 ± 0.048
3.282ArgThr: 3.282 ± 0.046
4.737ArgVal: 4.737 ± 0.058
1.015ArgTrp: 1.015 ± 0.026
2.067ArgTyr: 2.067 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
6.202SerAla: 6.202 ± 0.071
0.49SerCys: 0.49 ± 0.022
2.738SerAsp: 2.738 ± 0.048
2.735SerGlu: 2.735 ± 0.046
2.193SerPhe: 2.193 ± 0.039
5.316SerGly: 5.316 ± 0.063
1.211SerHis: 1.211 ± 0.028
2.653SerIle: 2.653 ± 0.038
1.812SerLys: 1.812 ± 0.034
5.548SerLeu: 5.548 ± 0.068
1.385SerMet: 1.385 ± 0.032
1.786SerAsn: 1.786 ± 0.039
2.744SerPro: 2.744 ± 0.04
1.956SerGln: 1.956 ± 0.045
3.397SerArg: 3.397 ± 0.046
3.355SerSer: 3.355 ± 0.057
2.962SerThr: 2.962 ± 0.047
3.989SerVal: 3.989 ± 0.05
0.773SerTrp: 0.773 ± 0.024
1.492SerTyr: 1.492 ± 0.03
0.0SerXaa: 0.0 ± 0.0
Thr
5.538ThrAla: 5.538 ± 0.071
0.458ThrCys: 0.458 ± 0.019
2.619ThrAsp: 2.619 ± 0.036
2.428ThrGlu: 2.428 ± 0.038
1.874ThrPhe: 1.874 ± 0.032
4.727ThrGly: 4.727 ± 0.062
1.052ThrHis: 1.052 ± 0.025
2.686ThrIle: 2.686 ± 0.044
1.493ThrLys: 1.493 ± 0.035
5.88ThrLeu: 5.88 ± 0.059
1.243ThrMet: 1.243 ± 0.029
1.495ThrAsn: 1.495 ± 0.037
3.245ThrPro: 3.245 ± 0.049
1.729ThrGln: 1.729 ± 0.031
3.383ThrArg: 3.383 ± 0.043
2.993ThrSer: 2.993 ± 0.049
2.859ThrThr: 2.859 ± 0.057
4.289ThrVal: 4.289 ± 0.064
0.696ThrTrp: 0.696 ± 0.022
1.356ThrTyr: 1.356 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
9.027ValAla: 9.027 ± 0.077
0.7ValCys: 0.7 ± 0.02
4.005ValAsp: 4.005 ± 0.051
4.152ValGlu: 4.152 ± 0.056
2.661ValPhe: 2.661 ± 0.042
5.423ValGly: 5.423 ± 0.064
1.507ValHis: 1.507 ± 0.031
3.58ValIle: 3.58 ± 0.054
2.594ValLys: 2.594 ± 0.042
7.861ValLeu: 7.861 ± 0.085
1.816ValMet: 1.816 ± 0.038
2.44ValAsn: 2.44 ± 0.044
3.914ValPro: 3.914 ± 0.052
2.619ValGln: 2.619 ± 0.041
5.106ValArg: 5.106 ± 0.062
4.179ValSer: 4.179 ± 0.049
4.352ValThr: 4.352 ± 0.059
5.874ValVal: 5.874 ± 0.062
0.857ValTrp: 0.857 ± 0.024
1.728ValTyr: 1.728 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.106TrpAla: 1.106 ± 0.03
0.133TrpCys: 0.133 ± 0.01
0.696TrpAsp: 0.696 ± 0.025
0.596TrpGlu: 0.596 ± 0.019
0.577TrpPhe: 0.577 ± 0.022
0.873TrpGly: 0.873 ± 0.027
0.377TrpHis: 0.377 ± 0.015
0.693TrpIle: 0.693 ± 0.023
0.492TrpLys: 0.492 ± 0.017
1.73TrpLeu: 1.73 ± 0.034
0.418TrpMet: 0.418 ± 0.016
0.521TrpAsn: 0.521 ± 0.02
0.616TrpPro: 0.616 ± 0.022
0.664TrpGln: 0.664 ± 0.022
1.166TrpArg: 1.166 ± 0.027
0.793TrpSer: 0.793 ± 0.027
0.755TrpThr: 0.755 ± 0.025
0.889TrpVal: 0.889 ± 0.027
0.258TrpTrp: 0.258 ± 0.014
0.37TrpTyr: 0.37 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.693TyrAla: 2.693 ± 0.043
0.259TyrCys: 0.259 ± 0.013
1.465TyrAsp: 1.465 ± 0.034
1.276TyrGlu: 1.276 ± 0.028
1.073TyrPhe: 1.073 ± 0.025
2.206TyrGly: 2.206 ± 0.04
0.561TyrHis: 0.561 ± 0.018
0.948TyrIle: 0.948 ± 0.022
0.876TyrLys: 0.876 ± 0.024
2.704TyrLeu: 2.704 ± 0.039
0.523TyrMet: 0.523 ± 0.018
0.846TyrAsn: 0.846 ± 0.024
1.33TyrPro: 1.33 ± 0.031
1.054TyrGln: 1.054 ± 0.027
1.981TyrArg: 1.981 ± 0.035
1.425TyrSer: 1.425 ± 0.029
1.397TyrThr: 1.397 ± 0.034
1.834TyrVal: 1.834 ± 0.033
0.423TyrTrp: 0.423 ± 0.016
0.777TyrTyr: 0.777 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4650 proteins (1599580 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski