Agromyces protaetiae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Agromyces

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3136 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P6FAI9|A0A4P6FAI9_9MICO ATP-binding cassette domain-containing protein OS=Agromyces protaetiae OX=2509455 GN=ET445_06055 PE=4 SV=1
MM1 pKa = 7.44HH2 pKa = 7.05SSIVRR7 pKa = 11.84GAAALAGLTLAFGLAACSTPSSAEE31 pKa = 3.78DD32 pKa = 3.36TASDD36 pKa = 3.51DD37 pKa = 3.66TAAGTFASGDD47 pKa = 3.58EE48 pKa = 4.16NTLVFATLPDD58 pKa = 3.86HH59 pKa = 7.17EE60 pKa = 5.57GADD63 pKa = 3.83QDD65 pKa = 4.16AQPIADD71 pKa = 4.86WIAAITGKK79 pKa = 10.28DD80 pKa = 3.39VEE82 pKa = 4.6FFEE85 pKa = 4.63ATDD88 pKa = 3.57YY89 pKa = 10.19TAVVQGLAAGQIDD102 pKa = 3.74IAQISAFTYY111 pKa = 9.98YY112 pKa = 10.04QAQNAGADD120 pKa = 3.51IVPIGAQITSEE131 pKa = 4.06GAEE134 pKa = 3.7PGYY137 pKa = 11.03YY138 pKa = 9.82SVALKK143 pKa = 10.85NPAATDD149 pKa = 3.59VTSLADD155 pKa = 3.58FADD158 pKa = 4.1RR159 pKa = 11.84PVCFVNPTSTSGRR172 pKa = 11.84AIPAGQLKK180 pKa = 10.34SAGVTVSEE188 pKa = 4.42EE189 pKa = 3.76NTVFGEE195 pKa = 4.14EE196 pKa = 4.19HH197 pKa = 7.45DD198 pKa = 3.96LTAAKK203 pKa = 9.88IAEE206 pKa = 4.42GVDD209 pKa = 3.7CQVGFAQDD217 pKa = 3.03VDD219 pKa = 4.01ADD221 pKa = 4.0PLIEE225 pKa = 4.79SGALEE230 pKa = 4.66EE231 pKa = 4.15IDD233 pKa = 4.51RR234 pKa = 11.84YY235 pKa = 10.31LVPAAPIVMQAGLPQALQDD254 pKa = 3.81QLIEE258 pKa = 4.86AIADD262 pKa = 3.69STQSSLTDD270 pKa = 3.23AGVEE274 pKa = 3.95LNEE277 pKa = 4.44FLTEE281 pKa = 3.54NWFGFGKK288 pKa = 10.43VDD290 pKa = 4.22DD291 pKa = 4.74SYY293 pKa = 11.57YY294 pKa = 11.18DD295 pKa = 3.64NIRR298 pKa = 11.84TVCDD302 pKa = 3.34EE303 pKa = 3.88VADD306 pKa = 3.89IVEE309 pKa = 4.28ACAAA313 pKa = 3.97

Molecular weight: 32.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P6F9C9|A0A4P6F9C9_9MICO Transcriptional regulator OS=Agromyces protaetiae OX=2509455 GN=ET445_04805 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3136

0

3136

1040253

32

2823

331.7

35.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.191 ± 0.07

0.465 ± 0.01

6.311 ± 0.038

5.841 ± 0.039

3.209 ± 0.03

9.124 ± 0.033

1.896 ± 0.019

4.308 ± 0.03

1.945 ± 0.033

9.987 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.501 ± 0.018

1.817 ± 0.028

5.54 ± 0.033

2.52 ± 0.022

7.492 ± 0.052

5.546 ± 0.03

5.74 ± 0.04

9.167 ± 0.046

1.516 ± 0.018

1.885 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski