Amino acid dipepetide frequency for Mesonia sp. HuA40

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.669AlaAla: 4.669 ± 0.101
0.633AlaCys: 0.633 ± 0.033
3.316AlaAsp: 3.316 ± 0.081
4.073AlaGlu: 4.073 ± 0.077
3.64AlaPhe: 3.64 ± 0.075
4.361AlaGly: 4.361 ± 0.092
1.287AlaHis: 1.287 ± 0.043
5.513AlaIle: 5.513 ± 0.096
5.22AlaLys: 5.22 ± 0.091
7.031AlaLeu: 7.031 ± 0.123
1.567AlaMet: 1.567 ± 0.051
3.818AlaAsn: 3.818 ± 0.098
1.938AlaPro: 1.938 ± 0.053
3.643AlaGln: 3.643 ± 0.074
2.205AlaArg: 2.205 ± 0.059
4.353AlaSer: 4.353 ± 0.075
3.478AlaThr: 3.478 ± 0.075
4.174AlaVal: 4.174 ± 0.082
0.649AlaTrp: 0.649 ± 0.032
2.864AlaTyr: 2.864 ± 0.068
0.0AlaXaa: 0.0 ± 0.0
Cys
0.445CysAla: 0.445 ± 0.026
0.093CysCys: 0.093 ± 0.014
0.408CysAsp: 0.408 ± 0.025
0.458CysGlu: 0.458 ± 0.03
0.38CysPhe: 0.38 ± 0.023
0.56CysGly: 0.56 ± 0.031
0.154CysHis: 0.154 ± 0.015
0.543CysIle: 0.543 ± 0.031
0.448CysLys: 0.448 ± 0.024
0.704CysLeu: 0.704 ± 0.03
0.108CysMet: 0.108 ± 0.012
0.363CysAsn: 0.363 ± 0.023
0.326CysPro: 0.326 ± 0.027
0.286CysGln: 0.286 ± 0.019
0.187CysArg: 0.187 ± 0.016
0.505CysSer: 0.505 ± 0.027
0.398CysThr: 0.398 ± 0.025
0.439CysVal: 0.439 ± 0.023
0.075CysTrp: 0.075 ± 0.011
0.3CysTyr: 0.3 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
3.581AspAla: 3.581 ± 0.071
0.409AspCys: 0.409 ± 0.022
2.446AspAsp: 2.446 ± 0.064
3.696AspGlu: 3.696 ± 0.082
3.752AspPhe: 3.752 ± 0.062
2.911AspGly: 2.911 ± 0.075
0.847AspHis: 0.847 ± 0.034
3.802AspIle: 3.802 ± 0.068
3.835AspLys: 3.835 ± 0.085
5.723AspLeu: 5.723 ± 0.088
0.946AspMet: 0.946 ± 0.034
2.713AspAsn: 2.713 ± 0.076
1.626AspPro: 1.626 ± 0.053
2.06AspGln: 2.06 ± 0.056
1.775AspArg: 1.775 ± 0.045
2.882AspSer: 2.882 ± 0.069
2.512AspThr: 2.512 ± 0.064
3.144AspVal: 3.144 ± 0.075
0.7AspTrp: 0.7 ± 0.031
2.76AspTyr: 2.76 ± 0.069
0.0AspXaa: 0.0 ± 0.0
Glu
4.74GluAla: 4.74 ± 0.084
0.268GluCys: 0.268 ± 0.018
3.526GluAsp: 3.526 ± 0.071
5.323GluGlu: 5.323 ± 0.1
2.86GluPhe: 2.86 ± 0.064
3.394GluGly: 3.394 ± 0.081
1.227GluHis: 1.227 ± 0.047
5.474GluIle: 5.474 ± 0.084
6.065GluLys: 6.065 ± 0.116
6.328GluLeu: 6.328 ± 0.095
1.533GluMet: 1.533 ± 0.041
4.766GluAsn: 4.766 ± 0.083
1.658GluPro: 1.658 ± 0.045
3.1GluGln: 3.1 ± 0.06
2.707GluArg: 2.707 ± 0.059
3.182GluSer: 3.182 ± 0.069
3.279GluThr: 3.279 ± 0.069
4.509GluVal: 4.509 ± 0.085
0.552GluTrp: 0.552 ± 0.024
2.27GluTyr: 2.27 ± 0.065
0.0GluXaa: 0.0 ± 0.0
Phe
3.132PheAla: 3.132 ± 0.072
0.476PheCys: 0.476 ± 0.027
3.043PheAsp: 3.043 ± 0.066
3.354PheGlu: 3.354 ± 0.069
2.758PhePhe: 2.758 ± 0.076
3.382PheGly: 3.382 ± 0.077
0.769PheHis: 0.769 ± 0.032
4.061PheIle: 4.061 ± 0.079
4.187PheLys: 4.187 ± 0.076
5.162PheLeu: 5.162 ± 0.104
1.075PheMet: 1.075 ± 0.037
3.328PheAsn: 3.328 ± 0.073
1.654PhePro: 1.654 ± 0.048
1.575PheGln: 1.575 ± 0.042
1.57PheArg: 1.57 ± 0.048
3.775PheSer: 3.775 ± 0.075
3.188PheThr: 3.188 ± 0.069
2.86PheVal: 2.86 ± 0.066
0.631PheTrp: 0.631 ± 0.031
2.27PheTyr: 2.27 ± 0.059
0.0PheXaa: 0.0 ± 0.0
Gly
4.103GlyAla: 4.103 ± 0.087
0.527GlyCys: 0.527 ± 0.032
3.089GlyAsp: 3.089 ± 0.08
3.74GlyGlu: 3.74 ± 0.067
3.626GlyPhe: 3.626 ± 0.084
4.165GlyGly: 4.165 ± 0.093
1.113GlyHis: 1.113 ± 0.035
4.872GlyIle: 4.872 ± 0.1
4.713GlyLys: 4.713 ± 0.079
5.939GlyLeu: 5.939 ± 0.105
1.436GlyMet: 1.436 ± 0.051
3.244GlyAsn: 3.244 ± 0.076
1.294GlyPro: 1.294 ± 0.044
2.032GlyGln: 2.032 ± 0.051
2.006GlyArg: 2.006 ± 0.056
3.686GlySer: 3.686 ± 0.084
3.387GlyThr: 3.387 ± 0.082
4.272GlyVal: 4.272 ± 0.096
0.671GlyTrp: 0.671 ± 0.032
2.562GlyTyr: 2.562 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.028HisAla: 1.028 ± 0.034
0.199HisCys: 0.199 ± 0.019
0.71HisAsp: 0.71 ± 0.032
0.959HisGlu: 0.959 ± 0.036
1.236HisPhe: 1.236 ± 0.048
0.942HisGly: 0.942 ± 0.039
0.481HisHis: 0.481 ± 0.03
1.467HisIle: 1.467 ± 0.043
1.348HisLys: 1.348 ± 0.042
2.185HisLeu: 2.185 ± 0.058
0.32HisMet: 0.32 ± 0.02
0.903HisAsn: 0.903 ± 0.034
0.921HisPro: 0.921 ± 0.039
1.043HisGln: 1.043 ± 0.039
0.655HisArg: 0.655 ± 0.031
1.009HisSer: 1.009 ± 0.035
0.962HisThr: 0.962 ± 0.035
0.875HisVal: 0.875 ± 0.036
0.237HisTrp: 0.237 ± 0.019
0.919HisTyr: 0.919 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
5.644IleAla: 5.644 ± 0.093
0.608IleCys: 0.608 ± 0.028
4.856IleAsp: 4.856 ± 0.08
5.408IleGlu: 5.408 ± 0.1
3.51IlePhe: 3.51 ± 0.083
4.826IleGly: 4.826 ± 0.098
1.222IleHis: 1.222 ± 0.044
5.445IleIle: 5.445 ± 0.092
5.976IleLys: 5.976 ± 0.102
7.031IleLeu: 7.031 ± 0.106
1.245IleMet: 1.245 ± 0.041
4.665IleAsn: 4.665 ± 0.085
3.147IlePro: 3.147 ± 0.064
2.89IleGln: 2.89 ± 0.062
2.584IleArg: 2.584 ± 0.06
4.883IleSer: 4.883 ± 0.08
4.097IleThr: 4.097 ± 0.076
4.003IleVal: 4.003 ± 0.077
0.655IleTrp: 0.655 ± 0.03
2.897IleTyr: 2.897 ± 0.066
0.0IleXaa: 0.0 ± 0.0
Lys
5.595LysAla: 5.595 ± 0.098
0.312LysCys: 0.312 ± 0.02
4.171LysAsp: 4.171 ± 0.083
6.36LysGlu: 6.36 ± 0.112
2.816LysPhe: 2.816 ± 0.076
4.061LysGly: 4.061 ± 0.07
1.542LysHis: 1.542 ± 0.046
6.29LysIle: 6.29 ± 0.097
7.348LysLys: 7.348 ± 0.128
6.926LysLeu: 6.926 ± 0.105
1.927LysMet: 1.927 ± 0.053
5.662LysAsn: 5.662 ± 0.105
2.684LysPro: 2.684 ± 0.07
3.839LysGln: 3.839 ± 0.071
3.322LysArg: 3.322 ± 0.069
4.382LysSer: 4.382 ± 0.077
4.595LysThr: 4.595 ± 0.095
4.31LysVal: 4.31 ± 0.091
0.708LysTrp: 0.708 ± 0.027
2.766LysTyr: 2.766 ± 0.063
0.0LysXaa: 0.0 ± 0.0
Leu
7.059LeuAla: 7.059 ± 0.127
0.664LeuCys: 0.664 ± 0.025
5.171LeuAsp: 5.171 ± 0.082
6.624LeuGlu: 6.624 ± 0.098
5.065LeuPhe: 5.065 ± 0.102
6.237LeuGly: 6.237 ± 0.113
1.754LeuHis: 1.754 ± 0.047
7.292LeuIle: 7.292 ± 0.109
8.393LeuLys: 8.393 ± 0.127
9.58LeuLeu: 9.58 ± 0.172
2.01LeuMet: 2.01 ± 0.061
6.264LeuAsn: 6.264 ± 0.098
4.068LeuPro: 4.068 ± 0.078
4.076LeuGln: 4.076 ± 0.083
3.586LeuArg: 3.586 ± 0.067
6.963LeuSer: 6.963 ± 0.096
4.9LeuThr: 4.9 ± 0.084
5.768LeuVal: 5.768 ± 0.091
0.86LeuTrp: 0.86 ± 0.04
3.335LeuTyr: 3.335 ± 0.071
0.0LeuXaa: 0.0 ± 0.0
Met
1.653MetAla: 1.653 ± 0.051
0.125MetCys: 0.125 ± 0.013
0.856MetAsp: 0.856 ± 0.034
1.009MetGlu: 1.009 ± 0.042
0.775MetPhe: 0.775 ± 0.031
1.468MetGly: 1.468 ± 0.045
0.463MetHis: 0.463 ± 0.023
1.333MetIle: 1.333 ± 0.04
1.884MetLys: 1.884 ± 0.043
2.071MetLeu: 2.071 ± 0.061
0.531MetMet: 0.531 ± 0.028
1.152MetAsn: 1.152 ± 0.038
0.842MetPro: 0.842 ± 0.034
1.148MetGln: 1.148 ± 0.041
0.993MetArg: 0.993 ± 0.035
1.265MetSer: 1.265 ± 0.041
0.919MetThr: 0.919 ± 0.032
1.25MetVal: 1.25 ± 0.037
0.131MetTrp: 0.131 ± 0.013
0.616MetTyr: 0.616 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.906AsnAla: 3.906 ± 0.072
0.454AsnCys: 0.454 ± 0.03
2.85AsnAsp: 2.85 ± 0.075
3.693AsnGlu: 3.693 ± 0.075
3.22AsnPhe: 3.22 ± 0.074
3.344AsnGly: 3.344 ± 0.085
1.228AsnHis: 1.228 ± 0.039
4.174AsnIle: 4.174 ± 0.071
4.618AsnLys: 4.618 ± 0.092
6.445AsnLeu: 6.445 ± 0.111
1.12AsnMet: 1.12 ± 0.039
3.638AsnAsn: 3.638 ± 0.1
2.883AsnPro: 2.883 ± 0.066
3.411AsnGln: 3.411 ± 0.079
2.316AsnArg: 2.316 ± 0.059
3.289AsnSer: 3.289 ± 0.074
3.475AsnThr: 3.475 ± 0.078
2.823AsnVal: 2.823 ± 0.072
0.835AsnTrp: 0.835 ± 0.033
3.052AsnTyr: 3.052 ± 0.067
0.0AsnXaa: 0.0 ± 0.0
Pro
2.186ProAla: 2.186 ± 0.064
0.228ProCys: 0.228 ± 0.018
1.96ProAsp: 1.96 ± 0.062
2.725ProGlu: 2.725 ± 0.063
1.82ProPhe: 1.82 ± 0.047
2.012ProGly: 2.012 ± 0.053
0.672ProHis: 0.672 ± 0.031
2.711ProIle: 2.711 ± 0.065
2.716ProLys: 2.716 ± 0.061
3.17ProLeu: 3.17 ± 0.06
0.695ProMet: 0.695 ± 0.03
2.373ProAsn: 2.373 ± 0.067
0.868ProPro: 0.868 ± 0.035
1.62ProGln: 1.62 ± 0.046
1.001ProArg: 1.001 ± 0.036
2.113ProSer: 2.113 ± 0.059
1.862ProThr: 1.862 ± 0.06
2.398ProVal: 2.398 ± 0.054
0.297ProTrp: 0.297 ± 0.019
1.435ProTyr: 1.435 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
3.267GlnAla: 3.267 ± 0.066
0.179GlnCys: 0.179 ± 0.017
2.338GlnAsp: 2.338 ± 0.057
3.687GlnGlu: 3.687 ± 0.092
2.006GlnPhe: 2.006 ± 0.052
2.239GlnGly: 2.239 ± 0.06
0.811GlnHis: 0.811 ± 0.038
3.34GlnIle: 3.34 ± 0.073
3.643GlnLys: 3.643 ± 0.07
4.574GlnLeu: 4.574 ± 0.095
0.986GlnMet: 0.986 ± 0.037
2.927GlnAsn: 2.927 ± 0.072
1.403GlnPro: 1.403 ± 0.045
2.285GlnGln: 2.285 ± 0.06
1.553GlnArg: 1.553 ± 0.049
2.153GlnSer: 2.153 ± 0.052
2.107GlnThr: 2.107 ± 0.057
2.813GlnVal: 2.813 ± 0.069
0.395GlnTrp: 0.395 ± 0.023
1.459GlnTyr: 1.459 ± 0.043
0.0GlnXaa: 0.0 ± 0.0
Arg
2.245ArgAla: 2.245 ± 0.051
0.194ArgCys: 0.194 ± 0.018
1.715ArgAsp: 1.715 ± 0.046
2.457ArgGlu: 2.457 ± 0.062
1.991ArgPhe: 1.991 ± 0.049
1.914ArgGly: 1.914 ± 0.05
0.61ArgHis: 0.61 ± 0.032
2.915ArgIle: 2.915 ± 0.055
3.079ArgLys: 3.079 ± 0.074
3.828ArgLeu: 3.828 ± 0.07
0.844ArgMet: 0.844 ± 0.039
1.955ArgAsn: 1.955 ± 0.053
1.132ArgPro: 1.132 ± 0.038
1.418ArgGln: 1.418 ± 0.048
1.296ArgArg: 1.296 ± 0.045
1.875ArgSer: 1.875 ± 0.054
1.683ArgThr: 1.683 ± 0.049
2.293ArgVal: 2.293 ± 0.057
0.381ArgTrp: 0.381 ± 0.022
1.713ArgTyr: 1.713 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
3.756SerAla: 3.756 ± 0.077
0.58SerCys: 0.58 ± 0.027
2.829SerAsp: 2.829 ± 0.056
3.341SerGlu: 3.341 ± 0.069
3.873SerPhe: 3.873 ± 0.087
4.003SerGly: 4.003 ± 0.083
0.988SerHis: 0.988 ± 0.039
5.027SerIle: 5.027 ± 0.079
4.447SerLys: 4.447 ± 0.072
6.605SerLeu: 6.605 ± 0.103
1.155SerMet: 1.155 ± 0.04
3.367SerAsn: 3.367 ± 0.078
2.071SerPro: 2.071 ± 0.05
2.423SerGln: 2.423 ± 0.064
1.988SerArg: 1.988 ± 0.047
3.877SerSer: 3.877 ± 0.069
3.223SerThr: 3.223 ± 0.063
3.586SerVal: 3.586 ± 0.073
0.658SerTrp: 0.658 ± 0.031
2.609SerTyr: 2.609 ± 0.067
0.0SerXaa: 0.0 ± 0.0
Thr
3.849ThrAla: 3.849 ± 0.103
0.37ThrCys: 0.37 ± 0.025
2.882ThrAsp: 2.882 ± 0.067
3.035ThrGlu: 3.035 ± 0.074
2.535ThrPhe: 2.535 ± 0.057
3.885ThrGly: 3.885 ± 0.085
1.075ThrHis: 1.075 ± 0.04
3.632ThrIle: 3.632 ± 0.079
3.453ThrLys: 3.453 ± 0.075
5.18ThrLeu: 5.18 ± 0.086
0.806ThrMet: 0.806 ± 0.035
3.022ThrAsn: 3.022 ± 0.076
2.497ThrPro: 2.497 ± 0.065
2.725ThrGln: 2.725 ± 0.065
1.697ThrArg: 1.697 ± 0.049
3.346ThrSer: 3.346 ± 0.067
3.228ThrThr: 3.228 ± 0.1
2.894ThrVal: 2.894 ± 0.073
0.515ThrTrp: 0.515 ± 0.026
2.301ThrTyr: 2.301 ± 0.058
0.0ThrXaa: 0.0 ± 0.0
Val
4.192ValAla: 4.192 ± 0.076
0.476ValCys: 0.476 ± 0.022
3.177ValAsp: 3.177 ± 0.08
3.748ValGlu: 3.748 ± 0.077
3.298ValPhe: 3.298 ± 0.069
3.728ValGly: 3.728 ± 0.09
1.02ValHis: 1.02 ± 0.039
4.412ValIle: 4.412 ± 0.092
4.548ValLys: 4.548 ± 0.083
5.907ValLeu: 5.907 ± 0.09
1.215ValMet: 1.215 ± 0.04
3.663ValAsn: 3.663 ± 0.082
2.08ValPro: 2.08 ± 0.051
2.107ValGln: 2.107 ± 0.054
1.977ValArg: 1.977 ± 0.054
3.864ValSer: 3.864 ± 0.066
2.88ValThr: 2.88 ± 0.075
4.043ValVal: 4.043 ± 0.087
0.523ValTrp: 0.523 ± 0.024
2.459ValTyr: 2.459 ± 0.067
0.0ValXaa: 0.0 ± 0.0
Trp
0.62TrpAla: 0.62 ± 0.029
0.096TrpCys: 0.096 ± 0.012
0.552TrpAsp: 0.552 ± 0.03
0.645TrpGlu: 0.645 ± 0.03
0.593TrpPhe: 0.593 ± 0.031
0.599TrpGly: 0.599 ± 0.031
0.24TrpHis: 0.24 ± 0.018
0.662TrpIle: 0.662 ± 0.027
0.699TrpLys: 0.699 ± 0.028
1.115TrpLeu: 1.115 ± 0.045
0.3TrpMet: 0.3 ± 0.017
0.557TrpAsn: 0.557 ± 0.026
0.256TrpPro: 0.256 ± 0.019
0.492TrpGln: 0.492 ± 0.026
0.411TrpArg: 0.411 ± 0.024
0.593TrpSer: 0.593 ± 0.028
0.404TrpThr: 0.404 ± 0.022
0.698TrpVal: 0.698 ± 0.033
0.145TrpTrp: 0.145 ± 0.015
0.448TrpTyr: 0.448 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.765TyrAla: 2.765 ± 0.064
0.291TyrCys: 0.291 ± 0.019
2.224TyrAsp: 2.224 ± 0.056
2.424TyrGlu: 2.424 ± 0.06
2.497TyrPhe: 2.497 ± 0.063
2.445TyrGly: 2.445 ± 0.057
0.897TyrHis: 0.897 ± 0.035
2.63TyrIle: 2.63 ± 0.066
2.997TyrLys: 2.997 ± 0.072
4.164TyrLeu: 4.164 ± 0.079
0.662TyrMet: 0.662 ± 0.033
2.395TyrAsn: 2.395 ± 0.071
1.512TyrPro: 1.512 ± 0.049
1.993TyrGln: 1.993 ± 0.054
1.708TyrArg: 1.708 ± 0.048
2.413TyrSer: 2.413 ± 0.068
2.352TyrThr: 2.352 ± 0.064
2.163TyrVal: 2.163 ± 0.059
0.504TyrTrp: 0.504 ± 0.028
1.867TyrTyr: 1.867 ± 0.061
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2381 proteins (784139 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski