Streptomyces dysideae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8992 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A117S2K3|A0A117S2K3_9ACTN Protease HtpX homolog OS=Streptomyces dysideae OX=909626 GN=htpX PE=3 SV=1
MM1 pKa = 7.51DD2 pKa = 3.01QWDD5 pKa = 4.29EE6 pKa = 4.23NRR8 pKa = 11.84QDD10 pKa = 3.04WTDD13 pKa = 3.34PDD15 pKa = 4.42LPEE18 pKa = 4.9GPPPGTRR25 pKa = 11.84TARR28 pKa = 11.84VAVVTAVAVVLCAVAGMGIWAWARR52 pKa = 11.84DD53 pKa = 3.97GGSSNAGSPGGSLPSAPYY71 pKa = 10.08GAEE74 pKa = 3.98TASGDD79 pKa = 3.55AAGSGEE85 pKa = 4.53VPSHH89 pKa = 6.4PCAAVSYY96 pKa = 9.96EE97 pKa = 4.23LAQEE101 pKa = 3.86WGLQHH106 pKa = 6.82DD107 pKa = 4.32TTMTEE112 pKa = 4.27PGCRR116 pKa = 11.84WAMQDD121 pKa = 3.83DD122 pKa = 3.96GRR124 pKa = 11.84TSFIVLRR131 pKa = 11.84YY132 pKa = 9.39SDD134 pKa = 4.79EE135 pKa = 5.1PPAQWDD141 pKa = 3.96SVSPIAVDD149 pKa = 4.11GVPSATSVKK158 pKa = 9.9VSNSICMVMWPTSYY172 pKa = 11.26GSANVSAIQGYY183 pKa = 8.48NAGGEE188 pKa = 4.38DD189 pKa = 3.8VCDD192 pKa = 3.45AAADD196 pKa = 4.08FTAAVAPNVPGG207 pKa = 3.56

Molecular weight: 21.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A101V2Z9|A0A101V2Z9_9ACTN 3-hydroxyacyl-CoA dehydrogenase OS=Streptomyces dysideae OX=909626 GN=AQJ91_09495 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.89GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8992

0

8992

2870677

29

13131

319.2

34.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.161 ± 0.041

0.816 ± 0.006

6.06 ± 0.018

5.766 ± 0.026

2.735 ± 0.013

9.238 ± 0.026

2.356 ± 0.013

3.243 ± 0.016

2.263 ± 0.022

10.225 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.752 ± 0.009

1.888 ± 0.015

6.008 ± 0.024

2.895 ± 0.014

7.955 ± 0.03

5.147 ± 0.02

6.309 ± 0.023

8.39 ± 0.025

1.592 ± 0.013

2.2 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski