Amino acid dipepetide frequency for alpha proteobacterium U9-1i

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.142AlaAla: 21.142 ± 0.212
1.322AlaCys: 1.322 ± 0.033
7.52AlaAsp: 7.52 ± 0.088
8.284AlaGlu: 8.284 ± 0.094
5.31AlaPhe: 5.31 ± 0.07
11.177AlaGly: 11.177 ± 0.109
2.836AlaHis: 2.836 ± 0.051
6.587AlaIle: 6.587 ± 0.086
4.086AlaLys: 4.086 ± 0.078
15.763AlaLeu: 15.763 ± 0.184
3.547AlaMet: 3.547 ± 0.055
3.702AlaAsn: 3.702 ± 0.057
8.098AlaPro: 8.098 ± 0.107
5.143AlaGln: 5.143 ± 0.067
11.354AlaArg: 11.354 ± 0.123
7.252AlaSer: 7.252 ± 0.084
6.232AlaThr: 6.232 ± 0.112
8.467AlaVal: 8.467 ± 0.091
1.942AlaTrp: 1.942 ± 0.043
2.859AlaTyr: 2.859 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
1.305CysAla: 1.305 ± 0.033
0.091CysCys: 0.091 ± 0.008
0.476CysAsp: 0.476 ± 0.021
0.414CysGlu: 0.414 ± 0.017
0.299CysPhe: 0.299 ± 0.017
0.84CysGly: 0.84 ± 0.028
0.173CysHis: 0.173 ± 0.013
0.32CysIle: 0.32 ± 0.015
0.178CysLys: 0.178 ± 0.013
0.641CysLeu: 0.641 ± 0.023
0.138CysMet: 0.138 ± 0.011
0.174CysAsn: 0.174 ± 0.013
0.382CysPro: 0.382 ± 0.019
0.196CysGln: 0.196 ± 0.012
0.493CysArg: 0.493 ± 0.02
0.377CysSer: 0.377 ± 0.018
0.383CysThr: 0.383 ± 0.018
0.64CysVal: 0.64 ± 0.027
0.115CysTrp: 0.115 ± 0.011
0.164CysTyr: 0.164 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
8.584AspAla: 8.584 ± 0.106
0.371AspCys: 0.371 ± 0.017
3.32AspAsp: 3.32 ± 0.069
3.493AspGlu: 3.493 ± 0.064
2.101AspPhe: 2.101 ± 0.049
5.384AspGly: 5.384 ± 0.087
1.149AspHis: 1.149 ± 0.031
2.632AspIle: 2.632 ± 0.051
1.436AspLys: 1.436 ± 0.037
5.725AspLeu: 5.725 ± 0.075
1.226AspMet: 1.226 ± 0.03
1.265AspAsn: 1.265 ± 0.04
3.335AspPro: 3.335 ± 0.049
1.73AspGln: 1.73 ± 0.037
4.076AspArg: 4.076 ± 0.069
2.06AspSer: 2.06 ± 0.042
2.469AspThr: 2.469 ± 0.051
4.499AspVal: 4.499 ± 0.053
1.041AspTrp: 1.041 ± 0.031
1.369AspTyr: 1.369 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
8.76GluAla: 8.76 ± 0.105
0.355GluCys: 0.355 ± 0.017
2.782GluAsp: 2.782 ± 0.059
3.083GluGlu: 3.083 ± 0.073
1.815GluPhe: 1.815 ± 0.042
4.599GluGly: 4.599 ± 0.062
1.259GluHis: 1.259 ± 0.032
3.347GluIle: 3.347 ± 0.055
1.75GluLys: 1.75 ± 0.049
5.169GluLeu: 5.169 ± 0.071
1.478GluMet: 1.478 ± 0.037
1.56GluAsn: 1.56 ± 0.034
2.737GluPro: 2.737 ± 0.056
2.142GluGln: 2.142 ± 0.043
5.78GluArg: 5.78 ± 0.082
2.499GluSer: 2.499 ± 0.049
3.511GluThr: 3.511 ± 0.055
3.597GluVal: 3.597 ± 0.058
0.763GluTrp: 0.763 ± 0.026
1.014GluTyr: 1.014 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
5.535PheAla: 5.535 ± 0.071
0.357PheCys: 0.357 ± 0.016
2.764PheAsp: 2.764 ± 0.045
2.406PheGlu: 2.406 ± 0.045
1.387PhePhe: 1.387 ± 0.04
3.549PheGly: 3.549 ± 0.058
0.722PheHis: 0.722 ± 0.024
1.581PheIle: 1.581 ± 0.04
0.952PheLys: 0.952 ± 0.027
3.166PheLeu: 3.166 ± 0.061
0.765PheMet: 0.765 ± 0.024
1.201PheAsn: 1.201 ± 0.032
1.466PhePro: 1.466 ± 0.037
1.0PheGln: 1.0 ± 0.031
2.173PheArg: 2.173 ± 0.045
2.198PheSer: 2.198 ± 0.044
2.058PheThr: 2.058 ± 0.045
2.903PheVal: 2.903 ± 0.048
0.56PheTrp: 0.56 ± 0.02
0.865PheTyr: 0.865 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
12.637GlyAla: 12.637 ± 0.123
0.696GlyCys: 0.696 ± 0.028
4.919GlyAsp: 4.919 ± 0.088
5.27GlyGlu: 5.27 ± 0.064
3.509GlyPhe: 3.509 ± 0.058
8.001GlyGly: 8.001 ± 0.118
1.57GlyHis: 1.57 ± 0.04
3.192GlyIle: 3.192 ± 0.061
2.566GlyLys: 2.566 ± 0.059
8.069GlyLeu: 8.069 ± 0.076
2.044GlyMet: 2.044 ± 0.038
1.794GlyAsn: 1.794 ± 0.072
3.69GlyPro: 3.69 ± 0.053
2.821GlyGln: 2.821 ± 0.05
6.322GlyArg: 6.322 ± 0.072
3.781GlySer: 3.781 ± 0.066
2.989GlyThr: 2.989 ± 0.057
7.354GlyVal: 7.354 ± 0.092
1.405GlyTrp: 1.405 ± 0.041
2.098GlyTyr: 2.098 ± 0.043
0.0GlyXaa: 0.0 ± 0.0
His
2.611HisAla: 2.611 ± 0.055
0.213HisCys: 0.213 ± 0.015
1.156HisAsp: 1.156 ± 0.026
1.113HisGlu: 1.113 ± 0.031
0.776HisPhe: 0.776 ± 0.025
1.855HisGly: 1.855 ± 0.044
0.463HisHis: 0.463 ± 0.022
0.819HisIle: 0.819 ± 0.025
0.457HisLys: 0.457 ± 0.019
1.802HisLeu: 1.802 ± 0.039
0.409HisMet: 0.409 ± 0.021
0.469HisAsn: 0.469 ± 0.021
1.156HisPro: 1.156 ± 0.031
0.534HisGln: 0.534 ± 0.022
1.288HisArg: 1.288 ± 0.037
0.843HisSer: 0.843 ± 0.023
0.81HisThr: 0.81 ± 0.026
1.487HisVal: 1.487 ± 0.04
0.336HisTrp: 0.336 ± 0.018
0.542HisTyr: 0.542 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
7.82IleAla: 7.82 ± 0.096
0.393IleCys: 0.393 ± 0.019
3.51IleAsp: 3.51 ± 0.052
3.509IleGlu: 3.509 ± 0.054
1.453IlePhe: 1.453 ± 0.041
4.554IleGly: 4.554 ± 0.075
0.762IleHis: 0.762 ± 0.026
1.995IleIle: 1.995 ± 0.044
1.181IleLys: 1.181 ± 0.04
3.586IleLeu: 3.586 ± 0.056
0.845IleMet: 0.845 ± 0.026
1.341IleAsn: 1.341 ± 0.039
1.887IlePro: 1.887 ± 0.041
1.13IleGln: 1.13 ± 0.032
2.715IleArg: 2.715 ± 0.055
2.589IleSer: 2.589 ± 0.045
2.4IleThr: 2.4 ± 0.048
4.221IleVal: 4.221 ± 0.067
0.603IleTrp: 0.603 ± 0.022
0.992IleTyr: 0.992 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
3.629LysAla: 3.629 ± 0.07
0.142LysCys: 0.142 ± 0.011
1.395LysAsp: 1.395 ± 0.046
1.276LysGlu: 1.276 ± 0.044
0.858LysPhe: 0.858 ± 0.03
2.077LysGly: 2.077 ± 0.05
0.663LysHis: 0.663 ± 0.025
1.415LysIle: 1.415 ± 0.037
1.229LysLys: 1.229 ± 0.048
3.124LysLeu: 3.124 ± 0.063
0.556LysMet: 0.556 ± 0.022
0.71LysAsn: 0.71 ± 0.026
1.862LysPro: 1.862 ± 0.041
0.901LysGln: 0.901 ± 0.031
2.66LysArg: 2.66 ± 0.062
1.503LysSer: 1.503 ± 0.038
1.506LysThr: 1.506 ± 0.039
1.73LysVal: 1.73 ± 0.049
0.313LysTrp: 0.313 ± 0.015
0.5LysTyr: 0.5 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
14.338LeuAla: 14.338 ± 0.153
0.726LeuCys: 0.726 ± 0.021
5.953LeuAsp: 5.953 ± 0.072
5.236LeuGlu: 5.236 ± 0.084
3.519LeuPhe: 3.519 ± 0.063
7.877LeuGly: 7.877 ± 0.086
1.707LeuHis: 1.707 ± 0.04
4.981LeuIle: 4.981 ± 0.07
3.148LeuLys: 3.148 ± 0.059
8.253LeuLeu: 8.253 ± 0.109
2.257LeuMet: 2.257 ± 0.04
2.837LeuAsn: 2.837 ± 0.059
4.621LeuPro: 4.621 ± 0.067
2.674LeuGln: 2.674 ± 0.04
7.235LeuArg: 7.235 ± 0.089
5.966LeuSer: 5.966 ± 0.083
5.539LeuThr: 5.539 ± 0.093
6.716LeuVal: 6.716 ± 0.081
1.167LeuTrp: 1.167 ± 0.033
2.006LeuTyr: 2.006 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
3.017MetAla: 3.017 ± 0.052
0.173MetCys: 0.173 ± 0.013
1.081MetAsp: 1.081 ± 0.031
1.058MetGlu: 1.058 ± 0.029
0.753MetPhe: 0.753 ± 0.028
1.788MetGly: 1.788 ± 0.045
0.434MetHis: 0.434 ± 0.021
1.109MetIle: 1.109 ± 0.03
0.843MetLys: 0.843 ± 0.025
2.431MetLeu: 2.431 ± 0.043
0.558MetMet: 0.558 ± 0.023
0.732MetAsn: 0.732 ± 0.025
1.226MetPro: 1.226 ± 0.032
0.817MetGln: 0.817 ± 0.028
2.163MetArg: 2.163 ± 0.042
1.675MetSer: 1.675 ± 0.037
1.525MetThr: 1.525 ± 0.037
1.347MetVal: 1.347 ± 0.033
0.241MetTrp: 0.241 ± 0.015
0.283MetTyr: 0.283 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
4.094AsnAla: 4.094 ± 0.065
0.221AsnCys: 0.221 ± 0.013
1.495AsnAsp: 1.495 ± 0.051
1.387AsnGlu: 1.387 ± 0.036
1.033AsnPhe: 1.033 ± 0.032
2.539AsnGly: 2.539 ± 0.07
0.452AsnHis: 0.452 ± 0.018
1.28AsnIle: 1.28 ± 0.037
0.537AsnLys: 0.537 ± 0.025
2.403AsnLeu: 2.403 ± 0.044
0.589AsnMet: 0.589 ± 0.024
0.762AsnAsn: 0.762 ± 0.035
1.773AsnPro: 1.773 ± 0.046
0.789AsnGln: 0.789 ± 0.025
1.727AsnArg: 1.727 ± 0.04
1.226AsnSer: 1.226 ± 0.037
1.29AsnThr: 1.29 ± 0.038
2.191AsnVal: 2.191 ± 0.05
0.444AsnTrp: 0.444 ± 0.019
0.658AsnTyr: 0.658 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
6.691ProAla: 6.691 ± 0.093
0.299ProCys: 0.299 ± 0.017
3.272ProAsp: 3.272 ± 0.05
3.435ProGlu: 3.435 ± 0.066
1.972ProPhe: 1.972 ± 0.042
4.213ProGly: 4.213 ± 0.066
1.074ProHis: 1.074 ± 0.032
2.45ProIle: 2.45 ± 0.049
1.555ProLys: 1.555 ± 0.037
4.569ProLeu: 4.569 ± 0.075
1.178ProMet: 1.178 ± 0.028
1.736ProAsn: 1.736 ± 0.043
3.249ProPro: 3.249 ± 0.083
1.724ProGln: 1.724 ± 0.043
3.45ProArg: 3.45 ± 0.056
2.912ProSer: 2.912 ± 0.047
2.771ProThr: 2.771 ± 0.052
3.457ProVal: 3.457 ± 0.053
0.708ProTrp: 0.708 ± 0.023
1.039ProTyr: 1.039 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
4.443GlnAla: 4.443 ± 0.066
0.233GlnCys: 0.233 ± 0.012
1.367GlnAsp: 1.367 ± 0.033
1.464GlnGlu: 1.464 ± 0.042
1.115GlnPhe: 1.115 ± 0.035
2.456GlnGly: 2.456 ± 0.044
0.641GlnHis: 0.641 ± 0.025
1.707GlnIle: 1.707 ± 0.062
0.825GlnLys: 0.825 ± 0.026
2.908GlnLeu: 2.908 ± 0.058
0.854GlnMet: 0.854 ± 0.028
0.874GlnAsn: 0.874 ± 0.027
1.63GlnPro: 1.63 ± 0.037
1.152GlnGln: 1.152 ± 0.038
2.992GlnArg: 2.992 ± 0.054
1.855GlnSer: 1.855 ± 0.041
1.996GlnThr: 1.996 ± 0.041
2.172GlnVal: 2.172 ± 0.081
0.467GlnTrp: 0.467 ± 0.02
0.584GlnTyr: 0.584 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
10.956ArgAla: 10.956 ± 0.116
0.531ArgCys: 0.531 ± 0.02
4.586ArgAsp: 4.586 ± 0.07
4.639ArgGlu: 4.639 ± 0.07
3.212ArgPhe: 3.212 ± 0.061
5.604ArgGly: 5.604 ± 0.072
1.394ArgHis: 1.394 ± 0.033
3.857ArgIle: 3.857 ± 0.058
2.022ArgLys: 2.022 ± 0.047
7.873ArgLeu: 7.873 ± 0.09
2.019ArgMet: 2.019 ± 0.043
1.968ArgAsn: 1.968 ± 0.044
3.714ArgPro: 3.714 ± 0.063
2.379ArgGln: 2.379 ± 0.044
6.368ArgArg: 6.368 ± 0.086
3.565ArgSer: 3.565 ± 0.056
3.883ArgThr: 3.883 ± 0.057
5.73ArgVal: 5.73 ± 0.067
1.068ArgTrp: 1.068 ± 0.029
1.809ArgTyr: 1.809 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
7.335SerAla: 7.335 ± 0.098
0.414SerCys: 0.414 ± 0.019
2.903SerAsp: 2.903 ± 0.05
2.952SerGlu: 2.952 ± 0.059
2.227SerPhe: 2.227 ± 0.045
5.229SerGly: 5.229 ± 0.076
0.901SerHis: 0.901 ± 0.026
2.5SerIle: 2.5 ± 0.049
1.339SerLys: 1.339 ± 0.039
4.901SerLeu: 4.901 ± 0.063
1.262SerMet: 1.262 ± 0.033
1.35SerAsn: 1.35 ± 0.034
2.727SerPro: 2.727 ± 0.05
1.544SerGln: 1.544 ± 0.04
3.543SerArg: 3.543 ± 0.054
2.679SerSer: 2.679 ± 0.056
2.526SerThr: 2.526 ± 0.056
3.812SerVal: 3.812 ± 0.064
0.771SerTrp: 0.771 ± 0.024
1.271SerTyr: 1.271 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
5.899ThrAla: 5.899 ± 0.089
0.349ThrCys: 0.349 ± 0.016
2.625ThrAsp: 2.625 ± 0.057
2.574ThrGlu: 2.574 ± 0.048
1.989ThrPhe: 1.989 ± 0.043
4.435ThrGly: 4.435 ± 0.069
1.063ThrHis: 1.063 ± 0.031
2.572ThrIle: 2.572 ± 0.045
1.284ThrLys: 1.284 ± 0.033
5.702ThrLeu: 5.702 ± 0.065
1.122ThrMet: 1.122 ± 0.032
1.41ThrAsn: 1.41 ± 0.047
3.773ThrPro: 3.773 ± 0.055
1.869ThrGln: 1.869 ± 0.132
3.695ThrArg: 3.695 ± 0.06
2.704ThrSer: 2.704 ± 0.053
2.682ThrThr: 2.682 ± 0.086
3.03ThrVal: 3.03 ± 0.05
0.692ThrTrp: 0.692 ± 0.026
1.185ThrTyr: 1.185 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
9.342ValAla: 9.342 ± 0.098
0.586ValCys: 0.586 ± 0.02
3.993ValAsp: 3.993 ± 0.062
4.483ValGlu: 4.483 ± 0.073
2.794ValPhe: 2.794 ± 0.052
5.538ValGly: 5.538 ± 0.072
1.228ValHis: 1.228 ± 0.035
3.633ValIle: 3.633 ± 0.062
1.846ValLys: 1.846 ± 0.046
7.116ValLeu: 7.116 ± 0.085
1.639ValMet: 1.639 ± 0.045
1.961ValAsn: 1.961 ± 0.04
2.73ValPro: 2.73 ± 0.043
2.06ValGln: 2.06 ± 0.043
5.813ValArg: 5.813 ± 0.08
4.433ValSer: 4.433 ± 0.059
4.266ValThr: 4.266 ± 0.067
5.345ValVal: 5.345 ± 0.079
1.035ValTrp: 1.035 ± 0.033
1.379ValTyr: 1.379 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.651TrpAla: 1.651 ± 0.04
0.135TrpCys: 0.135 ± 0.011
0.696TrpAsp: 0.696 ± 0.024
0.586TrpGlu: 0.586 ± 0.023
0.579TrpPhe: 0.579 ± 0.024
1.013TrpGly: 1.013 ± 0.029
0.234TrpHis: 0.234 ± 0.014
0.713TrpIle: 0.713 ± 0.025
0.368TrpLys: 0.368 ± 0.018
1.59TrpLeu: 1.59 ± 0.04
0.35TrpMet: 0.35 ± 0.016
0.447TrpAsn: 0.447 ± 0.019
0.748TrpPro: 0.748 ± 0.031
0.405TrpGln: 0.405 ± 0.017
1.668TrpArg: 1.668 ± 0.04
0.96TrpSer: 0.96 ± 0.032
0.846TrpThr: 0.846 ± 0.03
0.831TrpVal: 0.831 ± 0.026
0.234TrpTrp: 0.234 ± 0.013
0.24TrpTyr: 0.24 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.835TyrAla: 2.835 ± 0.051
0.193TyrCys: 0.193 ± 0.013
1.451TyrAsp: 1.451 ± 0.05
1.339TyrGlu: 1.339 ± 0.034
0.907TyrPhe: 0.907 ± 0.029
1.997TyrGly: 1.997 ± 0.042
0.413TyrHis: 0.413 ± 0.019
0.797TyrIle: 0.797 ± 0.026
0.449TyrLys: 0.449 ± 0.022
1.965TyrLeu: 1.965 ± 0.038
0.416TyrMet: 0.416 ± 0.019
0.596TyrAsn: 0.596 ± 0.026
0.948TyrPro: 0.948 ± 0.032
0.725TyrGln: 0.725 ± 0.024
1.664TyrArg: 1.664 ± 0.035
1.106TyrSer: 1.106 ± 0.033
0.974TyrThr: 0.974 ± 0.033
1.69TyrVal: 1.69 ± 0.039
0.389TyrTrp: 0.389 ± 0.018
0.528TyrTyr: 0.528 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4195 proteins (1222329 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski