Artemisia virus A

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1WZY7|I1WZY7_9VIRU N-terminal protein OS=Artemisia virus A OX=1133751 PE=4 SV=1
MM1 pKa = 7.44SSVIFAFGVSKK12 pKa = 9.23TYY14 pKa = 8.43PTIVEE19 pKa = 4.68KK20 pKa = 11.46YY21 pKa = 8.64CGSSKK26 pKa = 10.7KK27 pKa = 10.36VFFRR31 pKa = 11.84GLAPGIEE38 pKa = 4.32TSLTVDD44 pKa = 3.23SPIKK48 pKa = 10.74VKK50 pKa = 9.61VAKK53 pKa = 9.59WNRR56 pKa = 11.84DD57 pKa = 3.3SVDD60 pKa = 3.55EE61 pKa = 5.22LEE63 pKa = 4.47ITCKK67 pKa = 10.8CEE69 pKa = 3.7TCGAKK74 pKa = 10.28AFEE77 pKa = 4.13ILEE80 pKa = 4.18FKK82 pKa = 10.91YY83 pKa = 10.89ADD85 pKa = 3.64NGDD88 pKa = 4.65EE89 pKa = 3.85IWEE92 pKa = 4.44TEE94 pKa = 4.83VVADD98 pKa = 3.46WCSYY102 pKa = 11.43CEE104 pKa = 4.09TKK106 pKa = 10.72KK107 pKa = 10.95LFDD110 pKa = 4.2SGDD113 pKa = 3.72TEE115 pKa = 5.58HH116 pKa = 6.38YY117 pKa = 8.03TPCPSPPAPISNN129 pKa = 3.66

Molecular weight: 14.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1WZY8|I1WZY8_9VIRU Capsid protein OS=Artemisia virus A OX=1133751 PE=3 SV=1
MM1 pKa = 7.29TKK3 pKa = 10.35KK4 pKa = 10.51KK5 pKa = 7.71QTXSNNGNGQRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84TRR22 pKa = 11.84PIPLPSGAEE31 pKa = 3.77LGSVVRR37 pKa = 11.84APSAMSHH44 pKa = 5.28NVRR47 pKa = 11.84KK48 pKa = 9.81RR49 pKa = 11.84NQIIRR54 pKa = 11.84QSTLGEE60 pKa = 3.98MCIGTEE66 pKa = 4.11STPLLATHH74 pKa = 6.79ATANTEE80 pKa = 3.9VIGFVNIDD88 pKa = 3.5VRR90 pKa = 11.84NSTQLNRR97 pKa = 11.84LSIIGSAFSKK107 pKa = 10.57FRR109 pKa = 11.84VLKK112 pKa = 7.96MTAVYY117 pKa = 10.42SPEE120 pKa = 3.51IATNISGAIAMALIYY135 pKa = 10.79DD136 pKa = 4.03SADD139 pKa = 2.98VVAANWNIQRR149 pKa = 11.84ILATDD154 pKa = 3.59KK155 pKa = 11.03AVKK158 pKa = 10.31SNLWTMSPPIRR169 pKa = 11.84YY170 pKa = 9.55DD171 pKa = 2.98NRR173 pKa = 11.84LAAKK177 pKa = 8.84PWYY180 pKa = 9.72ISGTTAGVGAQNEE193 pKa = 4.29QTPVTIVYY201 pKa = 9.71GYY203 pKa = 10.32YY204 pKa = 10.58SNSTNIPTGRR214 pKa = 11.84IDD216 pKa = 3.06VHH218 pKa = 7.34YY219 pKa = 9.74EE220 pKa = 3.79VEE222 pKa = 4.45LVQPIAPLANTT233 pKa = 4.21

Molecular weight: 25.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1902

129

956

475.5

52.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.203 ± 0.686

1.577 ± 0.496

5.047 ± 0.542

7.203 ± 0.784

4.574 ± 0.732

6.782 ± 0.31

1.209 ± 0.106

5.047 ± 0.938

6.204 ± 0.543

7.729 ± 0.791

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.945 ± 0.163

3.523 ± 0.928

5.731 ± 0.647

3.628 ± 0.48

5.573 ± 0.6

9.727 ± 0.685

5.363 ± 1.06

6.887 ± 0.218

2.156 ± 0.214

2.787 ± 0.343
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski