Amino acid dipepetide frequency for Bacillus sp. V5-8f

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.476AlaAla: 6.476 ± 0.097	0.586AlaCys: 0.586 ± 0.025	3.412AlaAsp: 3.412 ± 0.063	4.889AlaGlu: 4.889 ± 0.082	3.297AlaPhe: 3.297 ± 0.061	5.889AlaGly: 5.889 ± 0.068	1.277AlaHis: 1.277 ± 0.034	5.923AlaIle: 5.923 ± 0.078	4.768AlaLys: 4.768 ± 0.068	7.118AlaLeu: 7.118 ± 0.093	2.093AlaMet: 2.093 ± 0.044	2.797AlaAsn: 2.797 ± 0.05	2.15AlaPro: 2.15 ± 0.051	2.224AlaGln: 2.224 ± 0.048	2.854AlaArg: 2.854 ± 0.047	4.306AlaSer: 4.306 ± 0.065	3.396AlaThr: 3.396 ± 0.061	5.553AlaVal: 5.553 ± 0.065	0.592AlaTrp: 0.592 ± 0.02	2.274AlaTyr: 2.274 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.445CysAla: 0.445 ± 0.02	0.098CysCys: 0.098 ± 0.01	0.382CysAsp: 0.382 ± 0.017	0.433CysGlu: 0.433 ± 0.02	0.335CysPhe: 0.335 ± 0.015	0.666CysGly: 0.666 ± 0.026	0.201CysHis: 0.201 ± 0.013	0.515CysIle: 0.515 ± 0.021	0.385CysLys: 0.385 ± 0.019	0.679CysLeu: 0.679 ± 0.024	0.179CysMet: 0.179 ± 0.013	0.309CysAsn: 0.309 ± 0.017	0.37CysPro: 0.37 ± 0.019	0.228CysGln: 0.228 ± 0.015	0.302CysArg: 0.302 ± 0.017	0.499CysSer: 0.499 ± 0.025	0.404CysThr: 0.404 ± 0.019	0.42CysVal: 0.42 ± 0.021	0.07CysTrp: 0.07 ± 0.007	0.254CysTyr: 0.254 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.153AspAla: 3.153 ± 0.062	0.379AspCys: 0.379 ± 0.02	2.272AspAsp: 2.272 ± 0.057	4.065AspGlu: 4.065 ± 0.07	2.456AspPhe: 2.456 ± 0.051	3.392AspGly: 3.392 ± 0.073	1.144AspHis: 1.144 ± 0.034	4.291AspIle: 4.291 ± 0.071	3.105AspLys: 3.105 ± 0.06	4.872AspLeu: 4.872 ± 0.07	1.353AspMet: 1.353 ± 0.033	1.855AspAsn: 1.855 ± 0.052	2.079AspPro: 2.079 ± 0.05	1.801AspGln: 1.801 ± 0.043	2.333AspArg: 2.333 ± 0.046	2.74AspSer: 2.74 ± 0.049	2.434AspThr: 2.434 ± 0.049	3.569AspVal: 3.569 ± 0.061	0.613AspTrp: 0.613 ± 0.026	2.033AspTyr: 2.033 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	5.474GluAla: 5.474 ± 0.076	0.38GluCys: 0.38 ± 0.018	3.685GluAsp: 3.685 ± 0.058	6.711GluGlu: 6.711 ± 0.105	2.686GluPhe: 2.686 ± 0.049	4.539GluGly: 4.539 ± 0.075	1.486GluHis: 1.486 ± 0.038	5.86GluIle: 5.86 ± 0.078	6.544GluLys: 6.544 ± 0.094	6.94GluLeu: 6.94 ± 0.084	2.293GluMet: 2.293 ± 0.042	3.761GluAsn: 3.761 ± 0.06	1.941GluPro: 1.941 ± 0.044	3.24GluGln: 3.24 ± 0.062	3.542GluArg: 3.542 ± 0.059	3.639GluSer: 3.639 ± 0.055	3.932GluThr: 3.932 ± 0.057	4.824GluVal: 4.824 ± 0.074	0.779GluTrp: 0.779 ± 0.028	2.255GluTyr: 2.255 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	3.014PheAla: 3.014 ± 0.051	0.353PheCys: 0.353 ± 0.02	2.283PheAsp: 2.283 ± 0.045	2.879PheGlu: 2.879 ± 0.044	2.348PhePhe: 2.348 ± 0.05	3.373PheGly: 3.373 ± 0.06	1.037PheHis: 1.037 ± 0.029	3.811PheIle: 3.811 ± 0.071	2.33PheLys: 2.33 ± 0.044	4.597PheLeu: 4.597 ± 0.078	1.177PheMet: 1.177 ± 0.035	1.88PheAsn: 1.88 ± 0.036	1.782PhePro: 1.782 ± 0.041	1.546PheGln: 1.546 ± 0.037	1.571PheArg: 1.571 ± 0.041	3.277PheSer: 3.277 ± 0.058	2.507PheThr: 2.507 ± 0.045	3.016PheVal: 3.016 ± 0.052	0.462PheTrp: 0.462 ± 0.022	1.671PheTyr: 1.671 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	5.035GlyAla: 5.035 ± 0.079	0.675GlyCys: 0.675 ± 0.024	3.297GlyAsp: 3.297 ± 0.054	4.628GlyGlu: 4.628 ± 0.071	3.466GlyPhe: 3.466 ± 0.056	5.081GlyGly: 5.081 ± 0.083	1.508GlyHis: 1.508 ± 0.033	6.322GlyIle: 6.322 ± 0.085	5.488GlyLys: 5.488 ± 0.079	6.676GlyLeu: 6.676 ± 0.093	2.195GlyMet: 2.195 ± 0.046	3.044GlyAsn: 3.044 ± 0.055	1.846GlyPro: 1.846 ± 0.042	2.425GlyGln: 2.425 ± 0.054	3.039GlyArg: 3.039 ± 0.05	4.272GlySer: 4.272 ± 0.069	4.253GlyThr: 4.253 ± 0.064	4.931GlyVal: 4.931 ± 0.07	0.821GlyTrp: 0.821 ± 0.024	2.886GlyTyr: 2.886 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	1.267HisAla: 1.267 ± 0.033	0.194HisCys: 0.194 ± 0.013	1.093HisAsp: 1.093 ± 0.029	1.436HisGlu: 1.436 ± 0.037	1.127HisPhe: 1.127 ± 0.033	1.497HisGly: 1.497 ± 0.03	0.608HisHis: 0.608 ± 0.027	1.545HisIle: 1.545 ± 0.036	1.089HisLys: 1.089 ± 0.031	1.985HisLeu: 1.985 ± 0.042	0.489HisMet: 0.489 ± 0.017	0.847HisAsn: 0.847 ± 0.026	1.232HisPro: 1.232 ± 0.036	0.765HisGln: 0.765 ± 0.027	0.839HisArg: 0.839 ± 0.025	1.302HisSer: 1.302 ± 0.032	1.071HisThr: 1.071 ± 0.031	1.403HisVal: 1.403 ± 0.033	0.194HisTrp: 0.194 ± 0.013	0.842HisTyr: 0.842 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.982IleAla: 5.982 ± 0.083	0.636IleCys: 0.636 ± 0.023	4.14IleAsp: 4.14 ± 0.059	5.622IleGlu: 5.622 ± 0.075	3.3IlePhe: 3.3 ± 0.064	6.092IleGly: 6.092 ± 0.073	1.759IleHis: 1.759 ± 0.032	5.902IleIle: 5.902 ± 0.082	4.858IleLys: 4.858 ± 0.069	7.143IleLeu: 7.143 ± 0.09	1.954IleMet: 1.954 ± 0.04	3.369IleAsn: 3.369 ± 0.047	3.406IlePro: 3.406 ± 0.054	2.85IleGln: 2.85 ± 0.053	3.214IleArg: 3.214 ± 0.055	5.433IleSer: 5.433 ± 0.07	4.407IleThr: 4.407 ± 0.062	5.36IleVal: 5.36 ± 0.071	0.624IleTrp: 0.624 ± 0.028	2.389IleTyr: 2.389 ± 0.041	0.0IleXaa: 0.0 ± 0.0
Lys	4.815LysAla: 4.815 ± 0.067	0.32LysCys: 0.32 ± 0.015	3.716LysAsp: 3.716 ± 0.054	6.586LysGlu: 6.586 ± 0.089	1.967LysPhe: 1.967 ± 0.035	4.76LysGly: 4.76 ± 0.063	1.359LysHis: 1.359 ± 0.034	4.78LysIle: 4.78 ± 0.071	5.925LysLys: 5.925 ± 0.079	5.864LysLeu: 5.864 ± 0.083	2.13LysMet: 2.13 ± 0.041	3.462LysAsn: 3.462 ± 0.053	2.432LysPro: 2.432 ± 0.046	3.031LysGln: 3.031 ± 0.064	3.276LysArg: 3.276 ± 0.054	3.57LysSer: 3.57 ± 0.056	3.671LysThr: 3.671 ± 0.058	4.573LysVal: 4.573 ± 0.064	0.827LysTrp: 0.827 ± 0.024	2.146LysTyr: 2.146 ± 0.048	0.0LysXaa: 0.0 ± 0.0
Leu	7.394LeuAla: 7.394 ± 0.086	0.644LeuCys: 0.644 ± 0.026	4.698LeuAsp: 4.698 ± 0.07	6.574LeuGlu: 6.574 ± 0.083	4.54LeuPhe: 4.54 ± 0.08	6.575LeuGly: 6.575 ± 0.087	1.956LeuHis: 1.956 ± 0.045	6.881LeuIle: 6.881 ± 0.086	6.553LeuLys: 6.553 ± 0.08	9.674LeuLeu: 9.674 ± 0.124	2.404LeuMet: 2.404 ± 0.043	4.198LeuAsn: 4.198 ± 0.066	4.034LeuPro: 4.034 ± 0.06	3.516LeuGln: 3.516 ± 0.056	3.794LeuArg: 3.794 ± 0.061	6.909LeuSer: 6.909 ± 0.09	5.326LeuThr: 5.326 ± 0.067	6.28LeuVal: 6.28 ± 0.086	0.778LeuTrp: 0.778 ± 0.027	3.039LeuTyr: 3.039 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	2.247MetAla: 2.247 ± 0.05	0.153MetCys: 0.153 ± 0.013	1.566MetAsp: 1.566 ± 0.037	2.153MetGlu: 2.153 ± 0.047	1.076MetPhe: 1.076 ± 0.033	1.909MetGly: 1.909 ± 0.042	0.447MetHis: 0.447 ± 0.02	2.044MetIle: 2.044 ± 0.041	2.356MetLys: 2.356 ± 0.048	2.581MetLeu: 2.581 ± 0.056	0.799MetMet: 0.799 ± 0.026	1.453MetAsn: 1.453 ± 0.033	1.048MetPro: 1.048 ± 0.03	0.844MetGln: 0.844 ± 0.029	1.086MetArg: 1.086 ± 0.036	1.602MetSer: 1.602 ± 0.04	1.389MetThr: 1.389 ± 0.037	1.869MetVal: 1.869 ± 0.04	0.166MetTrp: 0.166 ± 0.012	0.726MetTyr: 0.726 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.946AsnAla: 2.946 ± 0.052	0.333AsnCys: 0.333 ± 0.019	2.162AsnAsp: 2.162 ± 0.044	3.427AsnGlu: 3.427 ± 0.058	1.711AsnPhe: 1.711 ± 0.035	3.541AsnGly: 3.541 ± 0.071	1.066AsnHis: 1.066 ± 0.034	3.468AsnIle: 3.468 ± 0.054	2.963AsnLys: 2.963 ± 0.053	3.934AsnLeu: 3.934 ± 0.06	1.206AsnMet: 1.206 ± 0.033	2.063AsnAsn: 2.063 ± 0.056	2.269AsnPro: 2.269 ± 0.044	1.893AsnGln: 1.893 ± 0.045	2.091AsnArg: 2.091 ± 0.042	2.428AsnSer: 2.428 ± 0.046	2.165AsnThr: 2.165 ± 0.044	3.043AsnVal: 3.043 ± 0.05	0.498AsnTrp: 0.498 ± 0.02	1.445AsnTyr: 1.445 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	2.5ProAla: 2.5 ± 0.046	0.228ProCys: 0.228 ± 0.015	2.205ProAsp: 2.205 ± 0.047	3.23ProGlu: 3.23 ± 0.053	2.058ProPhe: 2.058 ± 0.042	2.773ProGly: 2.773 ± 0.052	0.768ProHis: 0.768 ± 0.028	2.838ProIle: 2.838 ± 0.051	2.22ProLys: 2.22 ± 0.046	3.48ProLeu: 3.48 ± 0.059	0.863ProMet: 0.863 ± 0.028	1.624ProAsn: 1.624 ± 0.036	1.088ProPro: 1.088 ± 0.035	1.179ProGln: 1.179 ± 0.034	1.265ProArg: 1.265 ± 0.029	2.357ProSer: 2.357 ± 0.048	1.819ProThr: 1.819 ± 0.038	3.094ProVal: 3.094 ± 0.055	0.376ProTrp: 0.376 ± 0.018	1.449ProTyr: 1.449 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	2.744GlnAla: 2.744 ± 0.057	0.2GlnCys: 0.2 ± 0.013	1.666GlnAsp: 1.666 ± 0.044	3.034GlnGlu: 3.034 ± 0.054	1.54GlnPhe: 1.54 ± 0.038	2.255GlnGly: 2.255 ± 0.045	0.713GlnHis: 0.713 ± 0.027	2.573GlnIle: 2.573 ± 0.049	2.727GlnLys: 2.727 ± 0.052	3.76GlnLeu: 3.76 ± 0.059	1.104GlnMet: 1.104 ± 0.032	1.646GlnAsn: 1.646 ± 0.039	1.3GlnPro: 1.3 ± 0.04	1.663GlnGln: 1.663 ± 0.046	1.485GlnArg: 1.485 ± 0.036	2.088GlnSer: 2.088 ± 0.055	1.949GlnThr: 1.949 ± 0.04	2.366GlnVal: 2.366 ± 0.048	0.365GlnTrp: 0.365 ± 0.021	1.288GlnTyr: 1.288 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	2.577ArgAla: 2.577 ± 0.05	0.273ArgCys: 0.273 ± 0.016	2.195ArgAsp: 2.195 ± 0.044	3.437ArgGlu: 3.437 ± 0.064	1.998ArgPhe: 1.998 ± 0.044	2.469ArgGly: 2.469 ± 0.044	0.819ArgHis: 0.819 ± 0.026	3.253ArgIle: 3.253 ± 0.054	3.306ArgLys: 3.306 ± 0.052	4.073ArgLeu: 4.073 ± 0.064	1.298ArgMet: 1.298 ± 0.037	2.136ArgAsn: 2.136 ± 0.043	1.405ArgPro: 1.405 ± 0.034	1.619ArgGln: 1.619 ± 0.034	1.93ArgArg: 1.93 ± 0.046	2.331ArgSer: 2.331 ± 0.047	2.093ArgThr: 2.093 ± 0.044	2.685ArgVal: 2.685 ± 0.048	0.392ArgTrp: 0.392 ± 0.021	1.57ArgTyr: 1.57 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	3.965SerAla: 3.965 ± 0.067	0.437SerCys: 0.437 ± 0.019	2.783SerAsp: 2.783 ± 0.054	4.037SerGlu: 4.037 ± 0.059	3.312SerPhe: 3.312 ± 0.052	4.696SerGly: 4.696 ± 0.071	1.246SerHis: 1.246 ± 0.036	5.136SerIle: 5.136 ± 0.071	3.772SerLys: 3.772 ± 0.061	6.365SerLeu: 6.365 ± 0.088	1.758SerMet: 1.758 ± 0.037	2.616SerAsn: 2.616 ± 0.058	2.29SerPro: 2.29 ± 0.052	2.087SerGln: 2.087 ± 0.042	2.616SerArg: 2.616 ± 0.049	4.059SerSer: 4.059 ± 0.076	3.08SerThr: 3.08 ± 0.048	4.396SerVal: 4.396 ± 0.07	0.644SerTrp: 0.644 ± 0.024	2.166SerTyr: 2.166 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	4.192ThrAla: 4.192 ± 0.063	0.326ThrCys: 0.326 ± 0.018	2.638ThrAsp: 2.638 ± 0.053	3.574ThrGlu: 3.574 ± 0.049	2.455ThrPhe: 2.455 ± 0.045	4.541ThrGly: 4.541 ± 0.066	1.036ThrHis: 1.036 ± 0.028	4.416ThrIle: 4.416 ± 0.066	3.192ThrLys: 3.192 ± 0.058	5.0ThrLeu: 5.0 ± 0.068	1.33ThrMet: 1.33 ± 0.037	2.387ThrAsn: 2.387 ± 0.045	2.239ThrPro: 2.239 ± 0.046	1.382ThrGln: 1.382 ± 0.03	1.915ThrArg: 1.915 ± 0.04	3.118ThrSer: 3.118 ± 0.054	2.71ThrThr: 2.71 ± 0.056	4.229ThrVal: 4.229 ± 0.073	0.495ThrTrp: 0.495 ± 0.022	1.791ThrTyr: 1.791 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	5.006ValAla: 5.006 ± 0.072	0.571ValCys: 0.571 ± 0.021	3.467ValAsp: 3.467 ± 0.055	4.764ValGlu: 4.764 ± 0.067	3.057ValPhe: 3.057 ± 0.049	4.605ValGly: 4.605 ± 0.067	1.39ValHis: 1.39 ± 0.034	5.62ValIle: 5.62 ± 0.066	4.779ValLys: 4.779 ± 0.067	6.608ValLeu: 6.608 ± 0.091	1.754ValMet: 1.754 ± 0.042	3.115ValAsn: 3.115 ± 0.052	2.818ValPro: 2.818 ± 0.045	2.374ValGln: 2.374 ± 0.043	2.722ValArg: 2.722 ± 0.047	4.841ValSer: 4.841 ± 0.076	4.077ValThr: 4.077 ± 0.069	4.874ValVal: 4.874 ± 0.077	0.591ValTrp: 0.591 ± 0.025	2.255ValTyr: 2.255 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.616TrpAla: 0.616 ± 0.024	0.074TrpCys: 0.074 ± 0.007	0.503TrpAsp: 0.503 ± 0.025	0.642TrpGlu: 0.642 ± 0.024	0.479TrpPhe: 0.479 ± 0.02	0.725TrpGly: 0.725 ± 0.026	0.201TrpHis: 0.201 ± 0.013	0.784TrpIle: 0.784 ± 0.028	0.704TrpLys: 0.704 ± 0.025	1.094TrpLeu: 1.094 ± 0.034	0.289TrpMet: 0.289 ± 0.016	0.569TrpAsn: 0.569 ± 0.022	0.272TrpPro: 0.272 ± 0.015	0.326TrpGln: 0.326 ± 0.018	0.408TrpArg: 0.408 ± 0.021	0.525TrpSer: 0.525 ± 0.021	0.476TrpThr: 0.476 ± 0.021	0.675TrpVal: 0.675 ± 0.027	0.119TrpTrp: 0.119 ± 0.009	0.311TrpTyr: 0.311 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.026TyrAla: 2.026 ± 0.039	0.315TyrCys: 0.315 ± 0.016	1.777TyrAsp: 1.777 ± 0.045	2.389TyrGlu: 2.389 ± 0.051	1.709TyrPhe: 1.709 ± 0.039	2.51TyrGly: 2.51 ± 0.044	0.802TyrHis: 0.802 ± 0.027	2.478TyrIle: 2.478 ± 0.051	2.148TyrLys: 2.148 ± 0.048	3.321TyrLeu: 3.321 ± 0.055	0.845TyrMet: 0.845 ± 0.03	1.52TyrAsn: 1.52 ± 0.04	1.412TyrPro: 1.412 ± 0.031	1.476TyrGln: 1.476 ± 0.037	1.576TyrArg: 1.576 ± 0.034	2.165TyrSer: 2.165 ± 0.046	1.806TyrThr: 1.806 ± 0.039	2.13TyrVal: 2.13 ± 0.043	0.385TyrTrp: 0.385 ± 0.018	1.249TyrTyr: 1.249 ± 0.04	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4122 proteins (1167400 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---