Amino acid dipepetide frequency for SAR116 cluster alpha proteobacterium HIMB100

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.715AlaAla: 13.715 ± 0.221	1.315AlaCys: 1.315 ± 0.046	7.665AlaAsp: 7.665 ± 0.116	7.98AlaGlu: 7.98 ± 0.151	3.925AlaPhe: 3.925 ± 0.076	9.831AlaGly: 9.831 ± 0.152	2.323AlaHis: 2.323 ± 0.062	5.326AlaIle: 5.326 ± 0.097	3.998AlaLys: 3.998 ± 0.092	10.962AlaLeu: 10.962 ± 0.147	2.87AlaMet: 2.87 ± 0.078	2.846AlaAsn: 2.846 ± 0.075	4.076AlaPro: 4.076 ± 0.092	4.043AlaGln: 4.043 ± 0.089	6.272AlaArg: 6.272 ± 0.115	6.506AlaSer: 6.506 ± 0.125	4.709AlaThr: 4.709 ± 0.088	8.126AlaVal: 8.126 ± 0.109	0.993AlaTrp: 0.993 ± 0.032	2.455AlaTyr: 2.455 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	1.113CysAla: 1.113 ± 0.038	0.146CysCys: 0.146 ± 0.015	0.737CysAsp: 0.737 ± 0.033	0.525CysGlu: 0.525 ± 0.029	0.55CysPhe: 0.55 ± 0.029	1.12CysGly: 1.12 ± 0.047	0.346CysHis: 0.346 ± 0.023	0.509CysIle: 0.509 ± 0.027	0.3CysLys: 0.3 ± 0.024	1.388CysLeu: 1.388 ± 0.05	0.227CysMet: 0.227 ± 0.017	0.296CysAsn: 0.296 ± 0.019	0.593CysPro: 0.593 ± 0.033	0.45CysGln: 0.45 ± 0.031	0.639CysArg: 0.639 ± 0.032	0.722CysSer: 0.722 ± 0.034	0.462CysThr: 0.462 ± 0.027	0.788CysVal: 0.788 ± 0.03	0.14CysTrp: 0.14 ± 0.013	0.306CysTyr: 0.306 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	5.734AspAla: 5.734 ± 0.1	0.698AspCys: 0.698 ± 0.033	3.589AspAsp: 3.589 ± 0.086	4.024AspGlu: 4.024 ± 0.071	2.275AspPhe: 2.275 ± 0.055	5.151AspGly: 5.151 ± 0.103	1.38AspHis: 1.38 ± 0.046	4.496AspIle: 4.496 ± 0.069	2.906AspLys: 2.906 ± 0.067	5.519AspLeu: 5.519 ± 0.091	1.872AspMet: 1.872 ± 0.056	1.916AspAsn: 1.916 ± 0.101	2.503AspPro: 2.503 ± 0.064	2.647AspGln: 2.647 ± 0.062	3.558AspArg: 3.558 ± 0.07	2.965AspSer: 2.965 ± 0.068	3.072AspThr: 3.072 ± 0.077	4.225AspVal: 4.225 ± 0.097	1.021AspTrp: 1.021 ± 0.036	1.893AspTyr: 1.893 ± 0.053	0.0AspXaa: 0.0 ± 0.0
Glu	6.734GluAla: 6.734 ± 0.112	0.35GluCys: 0.35 ± 0.022	3.108GluAsp: 3.108 ± 0.07	2.792GluGlu: 2.792 ± 0.08	1.909GluPhe: 1.909 ± 0.056	3.525GluGly: 3.525 ± 0.073	1.24GluHis: 1.24 ± 0.041	3.492GluIle: 3.492 ± 0.072	2.487GluLys: 2.487 ± 0.067	5.44GluLeu: 5.44 ± 0.087	1.756GluMet: 1.756 ± 0.052	1.857GluAsn: 1.857 ± 0.047	1.834GluPro: 1.834 ± 0.055	3.287GluGln: 3.287 ± 0.082	3.057GluArg: 3.057 ± 0.073	2.091GluSer: 2.091 ± 0.053	3.606GluThr: 3.606 ± 0.077	3.553GluVal: 3.553 ± 0.071	0.515GluTrp: 0.515 ± 0.027	1.012GluTyr: 1.012 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	4.312PheAla: 4.312 ± 0.083	0.61PheCys: 0.61 ± 0.031	2.858PheAsp: 2.858 ± 0.062	2.195PheGlu: 2.195 ± 0.057	1.589PhePhe: 1.589 ± 0.063	3.607PheGly: 3.607 ± 0.077	0.76PheHis: 0.76 ± 0.036	2.403PheIle: 2.403 ± 0.058	1.215PheLys: 1.215 ± 0.04	3.597PheLeu: 3.597 ± 0.08	0.991PheMet: 0.991 ± 0.034	1.251PheAsn: 1.251 ± 0.041	1.402PhePro: 1.402 ± 0.041	1.081PheGln: 1.081 ± 0.041	1.808PheArg: 1.808 ± 0.05	3.089PheSer: 3.089 ± 0.069	2.509PheThr: 2.509 ± 0.063	2.822PheVal: 2.822 ± 0.066	0.63PheTrp: 0.63 ± 0.031	1.101PheTyr: 1.101 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	7.659GlyAla: 7.659 ± 0.136	1.057GlyCys: 1.057 ± 0.044	3.969GlyAsp: 3.969 ± 0.114	3.79GlyGlu: 3.79 ± 0.071	3.718GlyPhe: 3.718 ± 0.084	6.687GlyGly: 6.687 ± 0.155	1.98GlyHis: 1.98 ± 0.064	4.316GlyIle: 4.316 ± 0.095	3.217GlyLys: 3.217 ± 0.08	9.359GlyLeu: 9.359 ± 0.141	2.294GlyMet: 2.294 ± 0.062	2.141GlyAsn: 2.141 ± 0.073	3.059GlyPro: 3.059 ± 0.075	3.912GlyGln: 3.912 ± 0.087	4.989GlyArg: 4.989 ± 0.089	4.271GlySer: 4.271 ± 0.11	4.144GlyThr: 4.144 ± 0.104	5.384GlyVal: 5.384 ± 0.114	1.37GlyTrp: 1.37 ± 0.048	2.432GlyTyr: 2.432 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	2.017HisAla: 2.017 ± 0.066	0.311HisCys: 0.311 ± 0.024	1.236HisAsp: 1.236 ± 0.048	1.055HisGlu: 1.055 ± 0.039	0.943HisPhe: 0.943 ± 0.037	1.689HisGly: 1.689 ± 0.053	0.768HisHis: 0.768 ± 0.041	1.583HisIle: 1.583 ± 0.057	0.898HisLys: 0.898 ± 0.036	2.711HisLeu: 2.711 ± 0.066	0.59HisMet: 0.59 ± 0.032	0.797HisAsn: 0.797 ± 0.032	1.332HisPro: 1.332 ± 0.045	1.036HisGln: 1.036 ± 0.041	1.121HisArg: 1.121 ± 0.043	1.135HisSer: 1.135 ± 0.039	1.024HisThr: 1.024 ± 0.04	1.374HisVal: 1.374 ± 0.048	0.268HisTrp: 0.268 ± 0.017	0.689HisTyr: 0.689 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	6.378IleAla: 6.378 ± 0.111	0.924IleCys: 0.924 ± 0.039	4.089IleAsp: 4.089 ± 0.068	3.483IleGlu: 3.483 ± 0.07	2.212IlePhe: 2.212 ± 0.057	4.808IleGly: 4.808 ± 0.098	1.148IleHis: 1.148 ± 0.042	3.352IleIle: 3.352 ± 0.073	2.105IleLys: 2.105 ± 0.056	5.443IleLeu: 5.443 ± 0.101	1.26IleMet: 1.26 ± 0.038	1.992IleAsn: 1.992 ± 0.064	2.296IlePro: 2.296 ± 0.073	1.772IleGln: 1.772 ± 0.051	2.94IleArg: 2.94 ± 0.075	4.308IleSer: 4.308 ± 0.075	3.613IleThr: 3.613 ± 0.092	3.63IleVal: 3.63 ± 0.073	0.764IleTrp: 0.764 ± 0.034	1.478IleTyr: 1.478 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	4.512LysAla: 4.512 ± 0.085	0.275LysCys: 0.275 ± 0.018	2.18LysAsp: 2.18 ± 0.058	1.489LysGlu: 1.489 ± 0.048	1.276LysPhe: 1.276 ± 0.046	2.886LysGly: 2.886 ± 0.07	0.728LysHis: 0.728 ± 0.035	2.474LysIle: 2.474 ± 0.06	1.814LysLys: 1.814 ± 0.072	3.934LysLeu: 3.934 ± 0.079	1.081LysMet: 1.081 ± 0.038	1.33LysAsn: 1.33 ± 0.05	1.892LysPro: 1.892 ± 0.061	1.996LysGln: 1.996 ± 0.062	2.29LysArg: 2.29 ± 0.065	2.571LysSer: 2.571 ± 0.064	2.757LysThr: 2.757 ± 0.062	2.105LysVal: 2.105 ± 0.062	0.463LysTrp: 0.463 ± 0.028	0.748LysTyr: 0.748 ± 0.04	0.0LysXaa: 0.0 ± 0.0
Leu	12.162LeuAla: 12.162 ± 0.156	1.202LeuCys: 1.202 ± 0.044	6.159LeuAsp: 6.159 ± 0.104	4.484LeuGlu: 4.484 ± 0.088	4.2LeuPhe: 4.2 ± 0.086	7.348LeuGly: 7.348 ± 0.114	2.193LeuHis: 2.193 ± 0.058	5.758LeuIle: 5.758 ± 0.094	4.006LeuLys: 4.006 ± 0.08	8.955LeuLeu: 8.955 ± 0.153	2.541LeuMet: 2.541 ± 0.057	3.631LeuAsn: 3.631 ± 0.082	5.38LeuPro: 5.38 ± 0.098	3.422LeuGln: 3.422 ± 0.065	5.51LeuArg: 5.51 ± 0.104	8.839LeuSer: 8.839 ± 0.13	5.974LeuThr: 5.974 ± 0.113	6.669LeuVal: 6.669 ± 0.108	1.103LeuTrp: 1.103 ± 0.042	2.29LeuTyr: 2.29 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	3.419MetAla: 3.419 ± 0.072	0.218MetCys: 0.218 ± 0.017	1.328MetAsp: 1.328 ± 0.04	0.928MetGlu: 0.928 ± 0.036	0.902MetPhe: 0.902 ± 0.032	1.983MetGly: 1.983 ± 0.06	0.522MetHis: 0.522 ± 0.027	1.495MetIle: 1.495 ± 0.047	1.252MetLys: 1.252 ± 0.041	2.586MetLeu: 2.586 ± 0.06	0.882MetMet: 0.882 ± 0.036	0.983MetAsn: 0.983 ± 0.037	1.359MetPro: 1.359 ± 0.041	0.941MetGln: 0.941 ± 0.037	1.619MetArg: 1.619 ± 0.049	1.92MetSer: 1.92 ± 0.05	2.017MetThr: 2.017 ± 0.049	1.816MetVal: 1.816 ± 0.053	0.215MetTrp: 0.215 ± 0.017	0.38MetTyr: 0.38 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	3.085AsnAla: 3.085 ± 0.089	0.419AsnCys: 0.419 ± 0.023	1.587AsnAsp: 1.587 ± 0.057	1.511AsnGlu: 1.511 ± 0.047	1.25AsnPhe: 1.25 ± 0.044	2.446AsnGly: 2.446 ± 0.063	0.676AsnHis: 0.676 ± 0.032	2.108AsnIle: 2.108 ± 0.07	1.307AsnLys: 1.307 ± 0.044	3.284AsnLeu: 3.284 ± 0.075	0.856AsnMet: 0.856 ± 0.035	1.107AsnAsn: 1.107 ± 0.062	1.85AsnPro: 1.85 ± 0.052	1.338AsnGln: 1.338 ± 0.044	1.905AsnArg: 1.905 ± 0.051	1.884AsnSer: 1.884 ± 0.082	1.694AsnThr: 1.694 ± 0.074	1.88AsnVal: 1.88 ± 0.068	0.482AsnTrp: 0.482 ± 0.028	0.82AsnTyr: 0.82 ± 0.039	0.0AsnXaa: 0.0 ± 0.0
Pro	4.876ProAla: 4.876 ± 0.104	0.397ProCys: 0.397 ± 0.026	3.789ProAsp: 3.789 ± 0.081	3.439ProGlu: 3.439 ± 0.087	1.985ProPhe: 1.985 ± 0.053	3.175ProGly: 3.175 ± 0.08	1.117ProHis: 1.117 ± 0.049	1.938ProIle: 1.938 ± 0.058	1.613ProLys: 1.613 ± 0.056	4.371ProLeu: 4.371 ± 0.082	0.944ProMet: 0.944 ± 0.04	1.211ProAsn: 1.211 ± 0.041	1.792ProPro: 1.792 ± 0.054	1.638ProGln: 1.638 ± 0.048	1.856ProArg: 1.856 ± 0.051	2.744ProSer: 2.744 ± 0.06	1.952ProThr: 1.952 ± 0.064	4.119ProVal: 4.119 ± 0.095	0.465ProTrp: 0.465 ± 0.029	1.116ProTyr: 1.116 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	5.063GlnAla: 5.063 ± 0.099	0.23GlnCys: 0.23 ± 0.016	2.031GlnAsp: 2.031 ± 0.051	1.446GlnGlu: 1.446 ± 0.037	1.538GlnPhe: 1.538 ± 0.048	2.44GlnGly: 2.44 ± 0.053	0.929GlnHis: 0.929 ± 0.035	3.148GlnIle: 3.148 ± 0.072	1.71GlnLys: 1.71 ± 0.051	4.414GlnLeu: 4.414 ± 0.09	1.387GlnMet: 1.387 ± 0.042	1.571GlnAsn: 1.571 ± 0.048	2.156GlnPro: 2.156 ± 0.053	1.967GlnGln: 1.967 ± 0.058	2.071GlnArg: 2.071 ± 0.056	2.384GlnSer: 2.384 ± 0.062	3.059GlnThr: 3.059 ± 0.066	2.442GlnVal: 2.442 ± 0.057	0.352GlnTrp: 0.352 ± 0.022	0.633GlnTyr: 0.633 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	5.248ArgAla: 5.248 ± 0.089	0.521ArgCys: 0.521 ± 0.027	3.069ArgAsp: 3.069 ± 0.073	2.681ArgGlu: 2.681 ± 0.076	2.477ArgPhe: 2.477 ± 0.061	3.402ArgGly: 3.402 ± 0.069	1.558ArgHis: 1.558 ± 0.051	3.017ArgIle: 3.017 ± 0.06	2.204ArgLys: 2.204 ± 0.056	7.034ArgLeu: 7.034 ± 0.127	1.424ArgMet: 1.424 ± 0.044	1.689ArgAsn: 1.689 ± 0.052	2.786ArgPro: 2.786 ± 0.075	3.051ArgGln: 3.051 ± 0.073	3.634ArgArg: 3.634 ± 0.094	3.088ArgSer: 3.088 ± 0.071	2.784ArgThr: 2.784 ± 0.069	3.366ArgVal: 3.366 ± 0.066	0.744ArgTrp: 0.744 ± 0.033	1.565ArgTyr: 1.565 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	7.009SerAla: 7.009 ± 0.123	0.769SerCys: 0.769 ± 0.035	4.001SerAsp: 4.001 ± 0.078	3.468SerGlu: 3.468 ± 0.076	2.922SerPhe: 2.922 ± 0.072	6.196SerGly: 6.196 ± 0.129	1.298SerHis: 1.298 ± 0.041	2.946SerIle: 2.946 ± 0.076	1.971SerLys: 1.971 ± 0.059	6.588SerLeu: 6.588 ± 0.104	1.537SerMet: 1.537 ± 0.05	1.638SerAsn: 1.638 ± 0.055	2.642SerPro: 2.642 ± 0.067	2.334SerGln: 2.334 ± 0.057	3.307SerArg: 3.307 ± 0.07	4.221SerSer: 4.221 ± 0.126	2.912SerThr: 2.912 ± 0.083	4.988SerVal: 4.988 ± 0.097	0.959SerTrp: 0.959 ± 0.038	1.733SerTyr: 1.733 ± 0.052	0.0SerXaa: 0.0 ± 0.0
Thr	6.815ThrAla: 6.815 ± 0.13	0.533ThrCys: 0.533 ± 0.033	4.0ThrAsp: 4.0 ± 0.092	2.987ThrGlu: 2.987 ± 0.058	1.717ThrPhe: 1.717 ± 0.053	5.719ThrGly: 5.719 ± 0.102	1.145ThrHis: 1.145 ± 0.044	3.213ThrIle: 3.213 ± 0.088	1.971ThrLys: 1.971 ± 0.05	5.067ThrLeu: 5.067 ± 0.086	1.136ThrMet: 1.136 ± 0.037	1.602ThrAsn: 1.602 ± 0.064	2.884ThrPro: 2.884 ± 0.062	1.874ThrGln: 1.874 ± 0.056	2.663ThrArg: 2.663 ± 0.059	3.611ThrSer: 3.611 ± 0.1	3.188ThrThr: 3.188 ± 0.104	3.906ThrVal: 3.906 ± 0.102	0.435ThrTrp: 0.435 ± 0.027	1.168ThrTyr: 1.168 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	6.63ValAla: 6.63 ± 0.116	0.897ValCys: 0.897 ± 0.034	3.837ValAsp: 3.837 ± 0.078	3.598ValGlu: 3.598 ± 0.075	2.79ValPhe: 2.79 ± 0.057	4.854ValGly: 4.854 ± 0.119	1.374ValHis: 1.374 ± 0.052	4.563ValIle: 4.563 ± 0.085	2.582ValLys: 2.582 ± 0.056	6.865ValLeu: 6.865 ± 0.102	2.207ValMet: 2.207 ± 0.063	2.469ValAsn: 2.469 ± 0.069	2.956ValPro: 2.956 ± 0.069	2.256ValGln: 2.256 ± 0.059	3.822ValArg: 3.822 ± 0.073	4.906ValSer: 4.906 ± 0.107	4.363ValThr: 4.363 ± 0.092	4.731ValVal: 4.731 ± 0.095	0.876ValTrp: 0.876 ± 0.038	1.514ValTyr: 1.514 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	1.164TrpAla: 1.164 ± 0.044	0.184TrpCys: 0.184 ± 0.016	0.622TrpAsp: 0.622 ± 0.03	0.427TrpGlu: 0.427 ± 0.022	0.522TrpPhe: 0.522 ± 0.026	0.816TrpGly: 0.816 ± 0.035	0.379TrpHis: 0.379 ± 0.023	0.577TrpIle: 0.577 ± 0.03	0.363TrpLys: 0.363 ± 0.022	1.57TrpLeu: 1.57 ± 0.054	0.382TrpMet: 0.382 ± 0.024	0.336TrpAsn: 0.336 ± 0.024	0.665TrpPro: 0.665 ± 0.029	0.788TrpGln: 0.788 ± 0.034	0.894TrpArg: 0.894 ± 0.037	0.781TrpSer: 0.781 ± 0.033	0.649TrpThr: 0.649 ± 0.032	0.725TrpVal: 0.725 ± 0.034	0.188TrpTrp: 0.188 ± 0.018	0.284TrpTyr: 0.284 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.29TyrAla: 2.29 ± 0.053	0.303TyrCys: 0.303 ± 0.018	1.591TyrAsp: 1.591 ± 0.051	1.344TyrGlu: 1.344 ± 0.039	1.131TyrPhe: 1.131 ± 0.039	2.067TyrGly: 2.067 ± 0.048	0.714TyrHis: 0.714 ± 0.033	1.278TyrIle: 1.278 ± 0.042	0.88TyrLys: 0.88 ± 0.032	2.738TyrLeu: 2.738 ± 0.069	0.529TyrMet: 0.529 ± 0.031	0.825TyrAsn: 0.825 ± 0.036	1.077TyrPro: 1.077 ± 0.038	1.139TyrGln: 1.139 ± 0.047	1.384TyrArg: 1.384 ± 0.043	1.402TyrSer: 1.402 ± 0.055	1.149TyrThr: 1.149 ± 0.056	1.442TyrVal: 1.442 ± 0.044	0.334TyrTrp: 0.334 ± 0.021	0.638TyrTyr: 0.638 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2334 proteins (749038 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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