Amino acid dipepetide frequency for Curvibacter sp. AEP1-3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.644AlaAla: 15.644 ± 0.173
1.259AlaCys: 1.259 ± 0.032
6.12AlaAsp: 6.12 ± 0.093
6.178AlaGlu: 6.178 ± 0.081
3.914AlaPhe: 3.914 ± 0.063
9.736AlaGly: 9.736 ± 0.126
2.576AlaHis: 2.576 ± 0.05
5.273AlaIle: 5.273 ± 0.064
5.086AlaLys: 5.086 ± 0.086
14.227AlaLeu: 14.227 ± 0.139
3.65AlaMet: 3.65 ± 0.058
3.406AlaAsn: 3.406 ± 0.085
5.517AlaPro: 5.517 ± 0.084
5.636AlaGln: 5.636 ± 0.073
7.064AlaArg: 7.064 ± 0.09
6.877AlaSer: 6.877 ± 0.102
6.206AlaThr: 6.206 ± 0.115
8.628AlaVal: 8.628 ± 0.087
1.876AlaTrp: 1.876 ± 0.044
2.521AlaTyr: 2.521 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
1.126CysAla: 1.126 ± 0.028
0.115CysCys: 0.115 ± 0.01
0.487CysAsp: 0.487 ± 0.024
0.508CysGlu: 0.508 ± 0.02
0.333CysPhe: 0.333 ± 0.018
0.89CysGly: 0.89 ± 0.031
0.26CysHis: 0.26 ± 0.015
0.476CysIle: 0.476 ± 0.017
0.321CysLys: 0.321 ± 0.017
0.868CysLeu: 0.868 ± 0.028
0.247CysMet: 0.247 ± 0.013
0.285CysAsn: 0.285 ± 0.016
0.467CysPro: 0.467 ± 0.023
0.259CysGln: 0.259 ± 0.014
0.52CysArg: 0.52 ± 0.018
0.575CysSer: 0.575 ± 0.02
0.516CysThr: 0.516 ± 0.019
0.739CysVal: 0.739 ± 0.026
0.129CysTrp: 0.129 ± 0.009
0.214CysTyr: 0.214 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
6.682AspAla: 6.682 ± 0.077
0.471AspCys: 0.471 ± 0.021
2.483AspAsp: 2.483 ± 0.053
2.921AspGlu: 2.921 ± 0.056
2.096AspPhe: 2.096 ± 0.047
4.137AspGly: 4.137 ± 0.071
1.023AspHis: 1.023 ± 0.029
2.625AspIle: 2.625 ± 0.052
2.142AspLys: 2.142 ± 0.047
5.218AspLeu: 5.218 ± 0.079
1.342AspMet: 1.342 ± 0.036
1.341AspAsn: 1.341 ± 0.037
2.472AspPro: 2.472 ± 0.046
1.736AspGln: 1.736 ± 0.037
2.814AspArg: 2.814 ± 0.053
2.665AspSer: 2.665 ± 0.051
2.789AspThr: 2.789 ± 0.078
4.065AspVal: 4.065 ± 0.047
0.907AspTrp: 0.907 ± 0.027
1.406AspTyr: 1.406 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
6.536GluAla: 6.536 ± 0.091
0.444GluCys: 0.444 ± 0.018
2.275GluAsp: 2.275 ± 0.043
2.643GluGlu: 2.643 ± 0.062
2.004GluPhe: 2.004 ± 0.042
3.637GluGly: 3.637 ± 0.058
1.287GluHis: 1.287 ± 0.034
2.692GluIle: 2.692 ± 0.046
2.24GluLys: 2.24 ± 0.048
5.818GluLeu: 5.818 ± 0.079
1.355GluMet: 1.355 ± 0.043
1.354GluAsn: 1.354 ± 0.035
2.293GluPro: 2.293 ± 0.045
2.602GluGln: 2.602 ± 0.054
3.853GluArg: 3.853 ± 0.072
3.024GluSer: 3.024 ± 0.056
2.619GluThr: 2.619 ± 0.059
4.191GluVal: 4.191 ± 0.055
0.745GluTrp: 0.745 ± 0.026
1.08GluTyr: 1.08 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
4.167PheAla: 4.167 ± 0.063
0.418PheCys: 0.418 ± 0.02
2.412PheAsp: 2.412 ± 0.04
2.103PheGlu: 2.103 ± 0.046
1.418PhePhe: 1.418 ± 0.037
3.162PheGly: 3.162 ± 0.049
0.729PheHis: 0.729 ± 0.027
1.555PheIle: 1.555 ± 0.038
1.539PheLys: 1.539 ± 0.031
2.956PheLeu: 2.956 ± 0.061
0.873PheMet: 0.873 ± 0.023
1.249PheAsn: 1.249 ± 0.033
1.529PhePro: 1.529 ± 0.043
1.189PheGln: 1.189 ± 0.026
1.663PheArg: 1.663 ± 0.037
2.433PheSer: 2.433 ± 0.053
2.045PheThr: 2.045 ± 0.049
2.857PheVal: 2.857 ± 0.049
0.578PheTrp: 0.578 ± 0.022
0.928PheTyr: 0.928 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
8.433GlyAla: 8.433 ± 0.158
0.816GlyCys: 0.816 ± 0.026
3.76GlyAsp: 3.76 ± 0.051
4.02GlyGlu: 4.02 ± 0.059
3.163GlyPhe: 3.163 ± 0.056
6.377GlyGly: 6.377 ± 0.128
1.854GlyHis: 1.854 ± 0.045
3.945GlyIle: 3.945 ± 0.056
3.819GlyLys: 3.819 ± 0.069
8.57GlyLeu: 8.57 ± 0.092
2.335GlyMet: 2.335 ± 0.052
2.474GlyAsn: 2.474 ± 0.097
2.707GlyPro: 2.707 ± 0.051
3.258GlyGln: 3.258 ± 0.046
4.508GlyArg: 4.508 ± 0.073
4.739GlySer: 4.739 ± 0.1
4.617GlyThr: 4.617 ± 0.14
6.552GlyVal: 6.552 ± 0.085
1.33GlyTrp: 1.33 ± 0.039
2.295GlyTyr: 2.295 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
2.633HisAla: 2.633 ± 0.06
0.303HisCys: 0.303 ± 0.019
1.054HisAsp: 1.054 ± 0.031
1.049HisGlu: 1.049 ± 0.029
0.906HisPhe: 0.906 ± 0.025
1.896HisGly: 1.896 ± 0.044
0.631HisHis: 0.631 ± 0.028
1.072HisIle: 1.072 ± 0.033
0.683HisLys: 0.683 ± 0.026
2.234HisLeu: 2.234 ± 0.046
0.566HisMet: 0.566 ± 0.019
0.573HisAsn: 0.573 ± 0.021
1.342HisPro: 1.342 ± 0.034
0.833HisGln: 0.833 ± 0.029
1.314HisArg: 1.314 ± 0.035
1.243HisSer: 1.243 ± 0.031
1.217HisThr: 1.217 ± 0.033
1.539HisVal: 1.539 ± 0.034
0.416HisTrp: 0.416 ± 0.018
0.623HisTyr: 0.623 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.169IleAla: 6.169 ± 0.076
0.45IleCys: 0.45 ± 0.018
2.821IleAsp: 2.821 ± 0.053
2.98IleGlu: 2.98 ± 0.062
1.357IlePhe: 1.357 ± 0.034
3.902IleGly: 3.902 ± 0.066
0.94IleHis: 0.94 ± 0.027
1.691IleIle: 1.691 ± 0.036
1.912IleLys: 1.912 ± 0.044
3.481IleLeu: 3.481 ± 0.058
0.848IleMet: 0.848 ± 0.026
1.682IleAsn: 1.682 ± 0.037
2.148IlePro: 2.148 ± 0.044
1.628IleGln: 1.628 ± 0.036
2.607IleArg: 2.607 ± 0.049
2.887IleSer: 2.887 ± 0.045
2.934IleThr: 2.934 ± 0.095
3.295IleVal: 3.295 ± 0.057
0.534IleTrp: 0.534 ± 0.021
1.027IleTyr: 1.027 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
5.087LysAla: 5.087 ± 0.08
0.199LysCys: 0.199 ± 0.013
2.164LysAsp: 2.164 ± 0.053
2.002LysGlu: 2.002 ± 0.043
1.232LysPhe: 1.232 ± 0.038
2.958LysGly: 2.958 ± 0.051
0.829LysHis: 0.829 ± 0.03
1.747LysIle: 1.747 ± 0.036
1.901LysLys: 1.901 ± 0.054
4.212LysLeu: 4.212 ± 0.068
1.026LysMet: 1.026 ± 0.03
1.234LysAsn: 1.234 ± 0.035
2.437LysPro: 2.437 ± 0.054
1.543LysGln: 1.543 ± 0.034
2.489LysArg: 2.489 ± 0.052
2.338LysSer: 2.338 ± 0.048
2.406LysThr: 2.406 ± 0.052
3.318LysVal: 3.318 ± 0.059
0.426LysTrp: 0.426 ± 0.019
0.762LysTyr: 0.762 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
13.175LeuAla: 13.175 ± 0.12
1.043LeuCys: 1.043 ± 0.031
5.639LeuAsp: 5.639 ± 0.077
5.482LeuGlu: 5.482 ± 0.094
3.321LeuPhe: 3.321 ± 0.056
8.271LeuGly: 8.271 ± 0.13
2.378LeuHis: 2.378 ± 0.052
4.509LeuIle: 4.509 ± 0.072
4.303LeuLys: 4.303 ± 0.069
10.825LeuLeu: 10.825 ± 0.139
2.761LeuMet: 2.761 ± 0.05
3.305LeuAsn: 3.305 ± 0.05
5.619LeuPro: 5.619 ± 0.074
4.997LeuGln: 4.997 ± 0.077
6.652LeuArg: 6.652 ± 0.089
6.628LeuSer: 6.628 ± 0.088
5.617LeuThr: 5.617 ± 0.137
7.918LeuVal: 7.918 ± 0.101
1.399LeuTrp: 1.399 ± 0.039
2.0LeuTyr: 2.0 ± 0.036
0.0LeuXaa: 0.0 ± 0.0
Met
3.265MetAla: 3.265 ± 0.059
0.192MetCys: 0.192 ± 0.012
1.342MetAsp: 1.342 ± 0.037
1.326MetGlu: 1.326 ± 0.034
0.79MetPhe: 0.79 ± 0.026
2.12MetGly: 2.12 ± 0.045
0.603MetHis: 0.603 ± 0.024
0.895MetIle: 0.895 ± 0.024
1.142MetLys: 1.142 ± 0.034
2.732MetLeu: 2.732 ± 0.044
0.629MetMet: 0.629 ± 0.024
0.921MetAsn: 0.921 ± 0.028
1.505MetPro: 1.505 ± 0.041
1.306MetGln: 1.306 ± 0.036
1.671MetArg: 1.671 ± 0.039
1.73MetSer: 1.73 ± 0.034
1.602MetThr: 1.602 ± 0.038
2.017MetVal: 2.017 ± 0.044
0.261MetTrp: 0.261 ± 0.013
0.415MetTyr: 0.415 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.806AsnAla: 3.806 ± 0.07
0.282AsnCys: 0.282 ± 0.015
1.495AsnAsp: 1.495 ± 0.044
1.405AsnGlu: 1.405 ± 0.034
1.121AsnPhe: 1.121 ± 0.031
2.512AsnGly: 2.512 ± 0.059
0.61AsnHis: 0.61 ± 0.022
1.445AsnIle: 1.445 ± 0.038
1.082AsnLys: 1.082 ± 0.032
3.022AsnLeu: 3.022 ± 0.057
0.729AsnMet: 0.729 ± 0.025
0.93AsnAsn: 0.93 ± 0.033
1.97AsnPro: 1.97 ± 0.045
1.139AsnGln: 1.139 ± 0.034
1.698AsnArg: 1.698 ± 0.034
1.694AsnSer: 1.694 ± 0.054
1.778AsnThr: 1.778 ± 0.065
2.173AsnVal: 2.173 ± 0.054
0.451AsnTrp: 0.451 ± 0.021
0.779AsnTyr: 0.779 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
6.311ProAla: 6.311 ± 0.092
0.359ProCys: 0.359 ± 0.017
2.851ProAsp: 2.851 ± 0.05
3.181ProGlu: 3.181 ± 0.056
1.71ProPhe: 1.71 ± 0.037
3.867ProGly: 3.867 ± 0.065
1.064ProHis: 1.064 ± 0.032
1.836ProIle: 1.836 ± 0.036
1.888ProLys: 1.888 ± 0.044
4.902ProLeu: 4.902 ± 0.064
1.305ProMet: 1.305 ± 0.035
1.323ProAsn: 1.323 ± 0.032
2.024ProPro: 2.024 ± 0.047
2.12ProGln: 2.12 ± 0.044
2.237ProArg: 2.237 ± 0.043
2.704ProSer: 2.704 ± 0.043
2.585ProThr: 2.585 ± 0.061
4.306ProVal: 4.306 ± 0.067
0.777ProTrp: 0.777 ± 0.031
1.069ProTyr: 1.069 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
5.657GlnAla: 5.657 ± 0.081
0.337GlnCys: 0.337 ± 0.013
1.87GlnAsp: 1.87 ± 0.042
1.912GlnGlu: 1.912 ± 0.042
1.421GlnPhe: 1.421 ± 0.034
3.243GlnGly: 3.243 ± 0.052
1.004GlnHis: 1.004 ± 0.035
1.837GlnIle: 1.837 ± 0.038
1.439GlnLys: 1.439 ± 0.034
4.434GlnLeu: 4.434 ± 0.061
1.05GlnMet: 1.05 ± 0.029
1.048GlnAsn: 1.048 ± 0.028
2.003GlnPro: 2.003 ± 0.047
2.036GlnGln: 2.036 ± 0.048
3.168GlnArg: 3.168 ± 0.051
2.588GlnSer: 2.588 ± 0.048
2.331GlnThr: 2.331 ± 0.056
3.283GlnVal: 3.283 ± 0.05
0.756GlnTrp: 0.756 ± 0.027
0.813GlnTyr: 0.813 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
6.264ArgAla: 6.264 ± 0.091
0.549ArgCys: 0.549 ± 0.022
3.013ArgAsp: 3.013 ± 0.063
3.489ArgGlu: 3.489 ± 0.06
2.474ArgPhe: 2.474 ± 0.05
3.84ArgGly: 3.84 ± 0.062
1.463ArgHis: 1.463 ± 0.037
3.186ArgIle: 3.186 ± 0.06
2.449ArgLys: 2.449 ± 0.048
6.59ArgLeu: 6.59 ± 0.087
1.87ArgMet: 1.87 ± 0.039
1.967ArgAsn: 1.967 ± 0.04
2.47ArgPro: 2.47 ± 0.046
2.527ArgGln: 2.527 ± 0.039
3.589ArgArg: 3.589 ± 0.065
3.463ArgSer: 3.463 ± 0.054
3.046ArgThr: 3.046 ± 0.048
4.358ArgVal: 4.358 ± 0.062
1.063ArgTrp: 1.063 ± 0.031
1.609ArgTyr: 1.609 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
7.078SerAla: 7.078 ± 0.1
0.449SerCys: 0.449 ± 0.021
2.952SerAsp: 2.952 ± 0.058
2.862SerGlu: 2.862 ± 0.051
2.209SerPhe: 2.209 ± 0.043
5.594SerGly: 5.594 ± 0.115
1.291SerHis: 1.291 ± 0.032
2.847SerIle: 2.847 ± 0.058
2.309SerLys: 2.309 ± 0.043
6.164SerLeu: 6.164 ± 0.075
1.597SerMet: 1.597 ± 0.035
1.89SerAsn: 1.89 ± 0.053
2.871SerPro: 2.871 ± 0.053
2.197SerGln: 2.197 ± 0.044
3.32SerArg: 3.32 ± 0.052
3.707SerSer: 3.707 ± 0.087
3.522SerThr: 3.522 ± 0.08
4.386SerVal: 4.386 ± 0.069
0.757SerTrp: 0.757 ± 0.026
1.332SerTyr: 1.332 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
6.163ThrAla: 6.163 ± 0.142
0.433ThrCys: 0.433 ± 0.02
2.719ThrAsp: 2.719 ± 0.061
2.757ThrGlu: 2.757 ± 0.046
1.929ThrPhe: 1.929 ± 0.04
4.901ThrGly: 4.901 ± 0.127
1.219ThrHis: 1.219 ± 0.032
2.426ThrIle: 2.426 ± 0.072
1.772ThrLys: 1.772 ± 0.043
6.713ThrLeu: 6.713 ± 0.196
1.205ThrMet: 1.205 ± 0.032
1.55ThrAsn: 1.55 ± 0.059
3.507ThrPro: 3.507 ± 0.06
2.211ThrGln: 2.211 ± 0.036
3.022ThrArg: 3.022 ± 0.051
3.149ThrSer: 3.149 ± 0.083
3.116ThrThr: 3.116 ± 0.089
4.68ThrVal: 4.68 ± 0.13
0.723ThrTrp: 0.723 ± 0.023
1.172ThrTyr: 1.172 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
9.224ValAla: 9.224 ± 0.098
0.768ValCys: 0.768 ± 0.028
4.098ValAsp: 4.098 ± 0.062
4.159ValGlu: 4.159 ± 0.069
2.836ValPhe: 2.836 ± 0.048
5.579ValGly: 5.579 ± 0.08
1.603ValHis: 1.603 ± 0.043
3.659ValIle: 3.659 ± 0.051
2.922ValLys: 2.922 ± 0.057
8.465ValLeu: 8.465 ± 0.1
2.098ValMet: 2.098 ± 0.043
2.434ValAsn: 2.434 ± 0.048
3.783ValPro: 3.783 ± 0.055
3.312ValGln: 3.312 ± 0.052
4.559ValArg: 4.559 ± 0.068
4.657ValSer: 4.657 ± 0.078
4.284ValThr: 4.284 ± 0.1
6.464ValVal: 6.464 ± 0.082
1.102ValTrp: 1.102 ± 0.033
1.62ValTyr: 1.62 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
1.491TrpAla: 1.491 ± 0.04
0.174TrpCys: 0.174 ± 0.013
0.65TrpAsp: 0.65 ± 0.024
0.578TrpGlu: 0.578 ± 0.023
0.56TrpPhe: 0.56 ± 0.023
1.008TrpGly: 1.008 ± 0.029
0.36TrpHis: 0.36 ± 0.015
0.696TrpIle: 0.696 ± 0.025
0.529TrpLys: 0.529 ± 0.021
2.003TrpLeu: 2.003 ± 0.052
0.49TrpMet: 0.49 ± 0.019
0.485TrpAsn: 0.485 ± 0.018
0.636TrpPro: 0.636 ± 0.024
0.738TrpGln: 0.738 ± 0.024
1.024TrpArg: 1.024 ± 0.031
0.887TrpSer: 0.887 ± 0.033
0.838TrpThr: 0.838 ± 0.026
1.154TrpVal: 1.154 ± 0.033
0.314TrpTrp: 0.314 ± 0.017
0.309TrpTyr: 0.309 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.481TyrAla: 2.481 ± 0.048
0.273TyrCys: 0.273 ± 0.014
1.131TyrAsp: 1.131 ± 0.032
1.14TyrGlu: 1.14 ± 0.033
1.01TyrPhe: 1.01 ± 0.031
1.942TyrGly: 1.942 ± 0.044
0.403TyrHis: 0.403 ± 0.018
0.877TyrIle: 0.877 ± 0.026
0.835TyrLys: 0.835 ± 0.027
2.408TyrLeu: 2.408 ± 0.048
0.486TyrMet: 0.486 ± 0.019
0.667TyrAsn: 0.667 ± 0.022
1.12TyrPro: 1.12 ± 0.033
0.962TyrGln: 0.962 ± 0.029
1.509TyrArg: 1.509 ± 0.037
1.346TyrSer: 1.346 ± 0.03
1.301TyrThr: 1.301 ± 0.04
1.702TyrVal: 1.702 ± 0.031
0.38TyrTrp: 0.38 ± 0.017
0.564TyrTyr: 0.564 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4049 proteins (1329744 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski