Amino acid dipepetide frequency for Enterobacter phage phiT5282H

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.674AlaAla: 9.674 ± 1.438
0.412AlaCys: 0.412 ± 0.17
5.763AlaAsp: 5.763 ± 1.037
5.763AlaGlu: 5.763 ± 1.025
3.293AlaPhe: 3.293 ± 0.57
8.233AlaGly: 8.233 ± 1.03
1.647AlaHis: 1.647 ± 0.342
3.705AlaIle: 3.705 ± 0.422
4.94AlaLys: 4.94 ± 0.636
9.88AlaLeu: 9.88 ± 1.373
2.779AlaMet: 2.779 ± 0.467
3.087AlaAsn: 3.087 ± 0.515
3.911AlaPro: 3.911 ± 0.659
4.014AlaGln: 4.014 ± 0.595
5.66AlaArg: 5.66 ± 0.876
6.175AlaSer: 6.175 ± 0.867
4.116AlaThr: 4.116 ± 0.729
6.792AlaVal: 6.792 ± 0.726
1.544AlaTrp: 1.544 ± 0.36
2.264AlaTyr: 2.264 ± 0.52
0.0AlaXaa: 0.0 ± 0.0
Cys
0.617CysAla: 0.617 ± 0.306
0.206CysCys: 0.206 ± 0.162
0.515CysAsp: 0.515 ± 0.331
0.412CysGlu: 0.412 ± 0.188
0.103CysPhe: 0.103 ± 0.103
0.926CysGly: 0.926 ± 0.298
0.103CysHis: 0.103 ± 0.118
0.926CysIle: 0.926 ± 0.315
0.412CysLys: 0.412 ± 0.158
0.515CysLeu: 0.515 ± 0.223
0.206CysMet: 0.206 ± 0.147
0.309CysAsn: 0.309 ± 0.178
0.72CysPro: 0.72 ± 0.248
0.412CysGln: 0.412 ± 0.246
1.235CysArg: 1.235 ± 0.492
0.309CysSer: 0.309 ± 0.181
0.72CysThr: 0.72 ± 0.29
0.617CysVal: 0.617 ± 0.21
0.206CysTrp: 0.206 ± 0.132
0.309CysTyr: 0.309 ± 0.182
0.0CysXaa: 0.0 ± 0.0
Asp
6.072AspAla: 6.072 ± 0.643
0.72AspCys: 0.72 ± 0.392
3.499AspAsp: 3.499 ± 0.781
4.631AspGlu: 4.631 ± 0.65
2.984AspPhe: 2.984 ± 0.67
5.351AspGly: 5.351 ± 0.733
1.132AspHis: 1.132 ± 0.339
3.911AspIle: 3.911 ± 0.655
3.087AspLys: 3.087 ± 0.618
6.175AspLeu: 6.175 ± 0.724
1.132AspMet: 1.132 ± 0.341
2.676AspAsn: 2.676 ± 0.458
1.544AspPro: 1.544 ± 0.366
2.058AspGln: 2.058 ± 0.42
2.984AspArg: 2.984 ± 0.534
2.779AspSer: 2.779 ± 0.407
4.425AspThr: 4.425 ± 0.733
3.499AspVal: 3.499 ± 0.509
1.132AspTrp: 1.132 ± 0.422
2.161AspTyr: 2.161 ± 0.466
0.0AspXaa: 0.0 ± 0.0
Glu
4.631GluAla: 4.631 ± 0.71
0.823GluCys: 0.823 ± 0.263
3.293GluAsp: 3.293 ± 0.604
3.19GluGlu: 3.19 ± 0.574
2.161GluPhe: 2.161 ± 0.51
3.602GluGly: 3.602 ± 0.52
1.338GluHis: 1.338 ± 0.401
4.014GluIle: 4.014 ± 0.616
3.087GluLys: 3.087 ± 0.46
5.866GluLeu: 5.866 ± 0.98
2.47GluMet: 2.47 ± 0.629
2.573GluAsn: 2.573 ± 0.474
3.087GluPro: 3.087 ± 0.554
2.676GluGln: 2.676 ± 0.495
3.705GluArg: 3.705 ± 0.555
3.293GluSer: 3.293 ± 0.521
4.425GluThr: 4.425 ± 0.643
3.705GluVal: 3.705 ± 0.708
1.235GluTrp: 1.235 ± 0.308
1.75GluTyr: 1.75 ± 0.463
0.0GluXaa: 0.0 ± 0.0
Phe
2.882PheAla: 2.882 ± 0.431
0.412PheCys: 0.412 ± 0.209
2.47PheAsp: 2.47 ± 0.423
2.573PheGlu: 2.573 ± 0.623
1.029PhePhe: 1.029 ± 0.275
1.955PheGly: 1.955 ± 0.437
0.206PheHis: 0.206 ± 0.143
2.161PheIle: 2.161 ± 0.48
2.264PheLys: 2.264 ± 0.512
2.47PheLeu: 2.47 ± 0.473
1.132PheMet: 1.132 ± 0.341
1.852PheAsn: 1.852 ± 0.415
1.235PhePro: 1.235 ± 0.375
1.235PheGln: 1.235 ± 0.332
2.573PheArg: 2.573 ± 0.4
2.573PheSer: 2.573 ± 0.355
3.602PheThr: 3.602 ± 0.611
1.955PheVal: 1.955 ± 0.457
0.515PheTrp: 0.515 ± 0.258
0.412PheTyr: 0.412 ± 0.166
0.0PheXaa: 0.0 ± 0.0
Gly
6.278GlyAla: 6.278 ± 0.588
1.132GlyCys: 1.132 ± 0.372
5.66GlyAsp: 5.66 ± 0.6
5.351GlyGlu: 5.351 ± 0.703
3.19GlyPhe: 3.19 ± 0.682
6.689GlyGly: 6.689 ± 1.011
0.823GlyHis: 0.823 ± 0.419
3.293GlyIle: 3.293 ± 0.567
4.631GlyLys: 4.631 ± 0.567
5.66GlyLeu: 5.66 ± 0.722
2.058GlyMet: 2.058 ± 0.425
3.293GlyAsn: 3.293 ± 0.52
1.852GlyPro: 1.852 ± 0.331
2.367GlyGln: 2.367 ± 0.507
4.219GlyArg: 4.219 ± 0.7
5.866GlySer: 5.866 ± 0.784
3.705GlyThr: 3.705 ± 0.704
5.763GlyVal: 5.763 ± 0.869
1.338GlyTrp: 1.338 ± 0.314
1.544GlyTyr: 1.544 ± 0.363
0.0GlyXaa: 0.0 ± 0.0
His
2.161HisAla: 2.161 ± 0.583
0.206HisCys: 0.206 ± 0.139
0.926HisAsp: 0.926 ± 0.569
1.029HisGlu: 1.029 ± 0.28
0.823HisPhe: 0.823 ± 0.326
1.75HisGly: 1.75 ± 0.352
0.309HisHis: 0.309 ± 0.13
1.132HisIle: 1.132 ± 0.369
0.515HisLys: 0.515 ± 0.207
1.955HisLeu: 1.955 ± 0.396
0.309HisMet: 0.309 ± 0.161
0.206HisAsn: 0.206 ± 0.143
0.823HisPro: 0.823 ± 0.287
0.926HisGln: 0.926 ± 0.324
1.338HisArg: 1.338 ± 0.329
1.029HisSer: 1.029 ± 0.329
0.617HisThr: 0.617 ± 0.28
1.647HisVal: 1.647 ± 0.355
0.309HisTrp: 0.309 ± 0.196
0.617HisTyr: 0.617 ± 0.282
0.0HisXaa: 0.0 ± 0.0
Ile
5.043IleAla: 5.043 ± 0.71
0.309IleCys: 0.309 ± 0.147
3.293IleAsp: 3.293 ± 0.629
3.293IleGlu: 3.293 ± 0.636
1.338IlePhe: 1.338 ± 0.308
4.425IleGly: 4.425 ± 0.737
0.617IleHis: 0.617 ± 0.286
2.058IleIle: 2.058 ± 0.484
3.19IleLys: 3.19 ± 0.552
3.293IleLeu: 3.293 ± 0.492
1.029IleMet: 1.029 ± 0.278
3.293IleAsn: 3.293 ± 0.56
1.955IlePro: 1.955 ± 0.522
2.058IleGln: 2.058 ± 0.532
3.499IleArg: 3.499 ± 0.587
4.219IleSer: 4.219 ± 0.698
3.705IleThr: 3.705 ± 0.701
2.882IleVal: 2.882 ± 0.515
0.617IleTrp: 0.617 ± 0.231
1.647IleTyr: 1.647 ± 0.407
0.0IleXaa: 0.0 ± 0.0
Lys
5.454LysAla: 5.454 ± 0.839
0.309LysCys: 0.309 ± 0.166
2.882LysAsp: 2.882 ± 0.525
3.19LysGlu: 3.19 ± 0.619
1.338LysPhe: 1.338 ± 0.426
2.264LysGly: 2.264 ± 0.473
0.823LysHis: 0.823 ± 0.292
2.779LysIle: 2.779 ± 0.468
5.557LysLys: 5.557 ± 0.824
5.351LysLeu: 5.351 ± 1.042
1.235LysMet: 1.235 ± 0.375
3.087LysAsn: 3.087 ± 0.6
2.264LysPro: 2.264 ± 0.395
1.544LysGln: 1.544 ± 0.417
3.499LysArg: 3.499 ± 0.789
3.19LysSer: 3.19 ± 0.517
3.705LysThr: 3.705 ± 0.632
3.293LysVal: 3.293 ± 0.554
0.72LysTrp: 0.72 ± 0.281
2.058LysTyr: 2.058 ± 0.378
0.0LysXaa: 0.0 ± 0.0
Leu
8.439LeuAla: 8.439 ± 1.27
1.544LeuCys: 1.544 ± 0.395
5.351LeuAsp: 5.351 ± 0.623
4.631LeuGlu: 4.631 ± 0.804
2.367LeuPhe: 2.367 ± 0.473
4.631LeuGly: 4.631 ± 0.957
1.235LeuHis: 1.235 ± 0.291
5.249LeuIle: 5.249 ± 0.627
4.837LeuLys: 4.837 ± 0.865
8.85LeuLeu: 8.85 ± 1.172
2.058LeuMet: 2.058 ± 0.352
4.734LeuAsn: 4.734 ± 0.623
3.705LeuPro: 3.705 ± 0.604
3.396LeuGln: 3.396 ± 0.743
7.307LeuArg: 7.307 ± 0.667
8.233LeuSer: 8.233 ± 0.968
7.924LeuThr: 7.924 ± 1.005
3.808LeuVal: 3.808 ± 0.782
1.132LeuTrp: 1.132 ± 0.313
2.161LeuTyr: 2.161 ± 0.469
0.0LeuXaa: 0.0 ± 0.0
Met
2.676MetAla: 2.676 ± 0.677
0.412MetCys: 0.412 ± 0.19
1.338MetAsp: 1.338 ± 0.407
0.926MetGlu: 0.926 ± 0.201
0.515MetPhe: 0.515 ± 0.246
1.544MetGly: 1.544 ± 0.41
0.515MetHis: 0.515 ± 0.242
1.544MetIle: 1.544 ± 0.343
1.544MetLys: 1.544 ± 0.384
1.955MetLeu: 1.955 ± 0.47
0.823MetMet: 0.823 ± 0.326
0.926MetAsn: 0.926 ± 0.31
1.235MetPro: 1.235 ± 0.379
0.617MetGln: 0.617 ± 0.221
1.852MetArg: 1.852 ± 0.446
2.47MetSer: 2.47 ± 0.481
1.441MetThr: 1.441 ± 0.391
1.647MetVal: 1.647 ± 0.389
0.206MetTrp: 0.206 ± 0.156
0.515MetTyr: 0.515 ± 0.227
0.0MetXaa: 0.0 ± 0.0
Asn
2.573AsnAla: 2.573 ± 0.548
0.309AsnCys: 0.309 ± 0.182
2.573AsnAsp: 2.573 ± 0.454
2.264AsnGlu: 2.264 ± 0.538
1.544AsnPhe: 1.544 ± 0.315
4.014AsnGly: 4.014 ± 0.581
1.338AsnHis: 1.338 ± 0.406
2.058AsnIle: 2.058 ± 0.517
1.75AsnLys: 1.75 ± 0.411
2.47AsnLeu: 2.47 ± 0.529
1.132AsnMet: 1.132 ± 0.269
1.955AsnAsn: 1.955 ± 0.514
2.676AsnPro: 2.676 ± 0.518
1.647AsnGln: 1.647 ± 0.407
3.087AsnArg: 3.087 ± 0.575
2.779AsnSer: 2.779 ± 0.552
1.955AsnThr: 1.955 ± 0.393
3.602AsnVal: 3.602 ± 0.642
0.617AsnTrp: 0.617 ± 0.229
1.029AsnTyr: 1.029 ± 0.287
0.0AsnXaa: 0.0 ± 0.0
Pro
4.631ProAla: 4.631 ± 0.579
0.309ProCys: 0.309 ± 0.159
4.014ProAsp: 4.014 ± 0.605
3.808ProGlu: 3.808 ± 0.58
2.264ProPhe: 2.264 ± 0.798
2.882ProGly: 2.882 ± 0.465
0.72ProHis: 0.72 ± 0.232
1.544ProIle: 1.544 ± 0.487
1.441ProLys: 1.441 ± 0.345
3.499ProLeu: 3.499 ± 0.572
1.029ProMet: 1.029 ± 0.229
0.515ProAsn: 0.515 ± 0.286
1.955ProPro: 1.955 ± 0.332
1.441ProGln: 1.441 ± 0.379
2.161ProArg: 2.161 ± 0.437
2.264ProSer: 2.264 ± 0.464
1.235ProThr: 1.235 ± 0.303
2.367ProVal: 2.367 ± 0.448
0.206ProTrp: 0.206 ± 0.138
2.161ProTyr: 2.161 ± 0.487
0.0ProXaa: 0.0 ± 0.0
Gln
4.219GlnAla: 4.219 ± 0.934
0.309GlnCys: 0.309 ± 0.193
1.441GlnAsp: 1.441 ± 0.32
1.75GlnGlu: 1.75 ± 0.499
1.441GlnPhe: 1.441 ± 0.381
2.779GlnGly: 2.779 ± 0.551
1.029GlnHis: 1.029 ± 0.295
2.367GlnIle: 2.367 ± 0.492
2.573GlnLys: 2.573 ± 0.393
4.528GlnLeu: 4.528 ± 0.583
0.515GlnMet: 0.515 ± 0.208
1.029GlnAsn: 1.029 ± 0.233
1.235GlnPro: 1.235 ± 0.236
2.573GlnGln: 2.573 ± 0.671
4.116GlnArg: 4.116 ± 0.761
2.367GlnSer: 2.367 ± 0.462
2.161GlnThr: 2.161 ± 0.447
2.573GlnVal: 2.573 ± 0.557
0.412GlnTrp: 0.412 ± 0.161
1.544GlnTyr: 1.544 ± 0.389
0.0GlnXaa: 0.0 ± 0.0
Arg
5.454ArgAla: 5.454 ± 0.724
0.515ArgCys: 0.515 ± 0.21
4.219ArgAsp: 4.219 ± 0.688
5.146ArgGlu: 5.146 ± 0.698
2.058ArgPhe: 2.058 ± 0.443
4.425ArgGly: 4.425 ± 0.75
1.852ArgHis: 1.852 ± 0.419
3.499ArgIle: 3.499 ± 0.58
3.705ArgLys: 3.705 ± 0.551
5.146ArgLeu: 5.146 ± 0.895
1.235ArgMet: 1.235 ± 0.336
2.779ArgAsn: 2.779 ± 0.524
2.161ArgPro: 2.161 ± 0.502
3.808ArgGln: 3.808 ± 0.56
4.528ArgArg: 4.528 ± 0.973
3.396ArgSer: 3.396 ± 0.64
4.014ArgThr: 4.014 ± 0.713
4.631ArgVal: 4.631 ± 0.606
1.029ArgTrp: 1.029 ± 0.4
2.47ArgTyr: 2.47 ± 0.679
0.0ArgXaa: 0.0 ± 0.0
Ser
7.718SerAla: 7.718 ± 1.02
0.309SerCys: 0.309 ± 0.156
4.322SerAsp: 4.322 ± 0.703
3.293SerGlu: 3.293 ± 0.587
2.058SerPhe: 2.058 ± 0.357
5.043SerGly: 5.043 ± 0.595
1.338SerHis: 1.338 ± 0.324
2.779SerIle: 2.779 ± 0.563
3.19SerLys: 3.19 ± 0.563
7.307SerLeu: 7.307 ± 1.0
1.132SerMet: 1.132 ± 0.338
3.087SerAsn: 3.087 ± 0.56
2.47SerPro: 2.47 ± 0.466
2.676SerGln: 2.676 ± 0.586
3.911SerArg: 3.911 ± 0.517
4.631SerSer: 4.631 ± 0.789
2.367SerThr: 2.367 ± 0.457
5.969SerVal: 5.969 ± 0.644
0.823SerTrp: 0.823 ± 0.316
1.235SerTyr: 1.235 ± 0.333
0.0SerXaa: 0.0 ± 0.0
Thr
6.689ThrAla: 6.689 ± 1.108
0.309ThrCys: 0.309 ± 0.159
4.425ThrAsp: 4.425 ± 0.75
4.219ThrGlu: 4.219 ± 0.62
2.367ThrPhe: 2.367 ± 0.496
6.381ThrGly: 6.381 ± 0.905
0.926ThrHis: 0.926 ± 0.293
3.087ThrIle: 3.087 ± 0.502
1.75ThrLys: 1.75 ± 0.545
5.66ThrLeu: 5.66 ± 0.829
1.544ThrMet: 1.544 ± 0.349
0.823ThrAsn: 0.823 ± 0.275
2.984ThrPro: 2.984 ± 0.46
3.087ThrGln: 3.087 ± 0.664
4.219ThrArg: 4.219 ± 0.65
3.396ThrSer: 3.396 ± 0.516
3.808ThrThr: 3.808 ± 0.847
3.911ThrVal: 3.911 ± 0.654
0.617ThrTrp: 0.617 ± 0.299
1.338ThrTyr: 1.338 ± 0.424
0.0ThrXaa: 0.0 ± 0.0
Val
5.66ValAla: 5.66 ± 0.827
0.412ValCys: 0.412 ± 0.179
4.014ValAsp: 4.014 ± 0.461
3.499ValGlu: 3.499 ± 0.594
2.573ValPhe: 2.573 ± 0.581
4.014ValGly: 4.014 ± 0.625
1.132ValHis: 1.132 ± 0.403
3.396ValIle: 3.396 ± 0.797
3.602ValLys: 3.602 ± 0.644
6.792ValLeu: 6.792 ± 0.861
1.852ValMet: 1.852 ± 0.468
3.293ValAsn: 3.293 ± 0.597
2.161ValPro: 2.161 ± 0.299
2.47ValGln: 2.47 ± 0.438
3.396ValArg: 3.396 ± 0.661
4.528ValSer: 4.528 ± 0.72
4.94ValThr: 4.94 ± 0.768
3.19ValVal: 3.19 ± 0.458
0.823ValTrp: 0.823 ± 0.279
1.75ValTyr: 1.75 ± 0.443
0.0ValXaa: 0.0 ± 0.0
Trp
1.029TrpAla: 1.029 ± 0.373
0.206TrpCys: 0.206 ± 0.162
0.412TrpAsp: 0.412 ± 0.168
0.926TrpGlu: 0.926 ± 0.27
0.823TrpPhe: 0.823 ± 0.321
0.823TrpGly: 0.823 ± 0.297
0.72TrpHis: 0.72 ± 0.231
0.926TrpIle: 0.926 ± 0.37
1.029TrpLys: 1.029 ± 0.361
2.264TrpLeu: 2.264 ± 0.55
0.103TrpMet: 0.103 ± 0.106
0.617TrpAsn: 0.617 ± 0.264
0.72TrpPro: 0.72 ± 0.234
0.823TrpGln: 0.823 ± 0.281
0.926TrpArg: 0.926 ± 0.301
0.515TrpSer: 0.515 ± 0.215
0.412TrpThr: 0.412 ± 0.161
0.515TrpVal: 0.515 ± 0.208
0.309TrpTrp: 0.309 ± 0.168
0.309TrpTyr: 0.309 ± 0.248
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.75TyrAla: 1.75 ± 0.363
0.617TyrCys: 0.617 ± 0.323
1.544TyrAsp: 1.544 ± 0.451
1.132TyrGlu: 1.132 ± 0.39
1.235TyrPhe: 1.235 ± 0.385
2.984TyrGly: 2.984 ± 0.594
0.823TyrHis: 0.823 ± 0.253
1.338TyrIle: 1.338 ± 0.311
1.441TyrLys: 1.441 ± 0.397
1.955TyrLeu: 1.955 ± 0.532
0.823TyrMet: 0.823 ± 0.259
1.235TyrAsn: 1.235 ± 0.426
1.75TyrPro: 1.75 ± 0.383
1.132TyrGln: 1.132 ± 0.327
1.852TyrArg: 1.852 ± 0.537
1.647TyrSer: 1.647 ± 0.413
2.264TyrThr: 2.264 ± 0.496
1.132TyrVal: 1.132 ± 0.252
0.617TyrTrp: 0.617 ± 0.219
1.029TyrTyr: 1.029 ± 0.376
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 39 proteins (9718 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski