Sinocyclocheilus grahami (Dianchi golden-line fish) (Barbus grahami)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai;

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102128 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A672RYX2|A0A672RYX2_SINGR Isoform of A0A672RXC5 Myotubularin-related protein 5-like OS=Sinocyclocheilus grahami OX=75366 GN=LOC107581285 PE=3 SV=1
KKK2 pKa = 10.5HH3 pKa = 6.07ISEEE7 pKa = 4.41KKK9 pKa = 7.93TGDDD13 pKa = 3.53SFCPEEE19 pKa = 5.15GPTDDD24 pKa = 4.03KK25 pKa = 10.76CTSGQSEEE33 pKa = 4.19LGDDD37 pKa = 3.63SSSGQLEEE45 pKa = 4.07TAEEE49 pKa = 4.45FTTDDD54 pKa = 2.94SGLTGEEE61 pKa = 4.35SSSGQLGPTAEEE73 pKa = 4.88FTTDDD78 pKa = 2.94SGLTGEEE85 pKa = 4.35SSSGQLGPTAEEE97 pKa = 4.88FTTDDD102 pKa = 2.94SGLTGEEE109 pKa = 4.35SSSGQLGPTAEEE121 pKa = 5.2FTTDDD126 pKa = 3.17SGLTGEEE133 pKa = 4.35SSSGQLGPTAEEE145 pKa = 5.2FTTDDD150 pKa = 3.17SGLTGEEE157 pKa = 4.35SSSGQLGPTAEEE169 pKa = 5.2FTTDDD174 pKa = 3.17SGLTGEEE181 pKa = 4.35SSSGQLGPTAEEE193 pKa = 5.2FTTDDD198 pKa = 3.17SGLTGEEE205 pKa = 4.35SSSGQLGPTAEEE217 pKa = 5.2FTTDDD222 pKa = 3.17SGLTGEEE229 pKa = 4.35SSSGQLGPTAEEE241 pKa = 5.2FTTDDD246 pKa = 3.17SGLTGEEE253 pKa = 4.35SSSGQLGPTAEEE265 pKa = 5.2FTTDDD270 pKa = 3.17SGLTGEEE277 pKa = 4.35SSSGQLGPTAEEE289 pKa = 5.2FTTDDD294 pKa = 3.17SGLTGEEE301 pKa = 4.35SSSGQLGPTAEEE313 pKa = 5.2FTTDDD318 pKa = 3.17SGLTGEEE325 pKa = 4.35SSSGQLGPTAEEE337 pKa = 5.2FTTDDD342 pKa = 3.17SGLTGEEE349 pKa = 4.35SSSGQLGPTAEEE361 pKa = 5.2FTTDDD366 pKa = 3.17SGLTGEEE373 pKa = 4.35SSSGQLGPTAEEE385 pKa = 5.2FTTDDD390 pKa = 3.17SGLTGEEE397 pKa = 4.35SSSGQLGPTAEEE409 pKa = 5.2FTTDDD414 pKa = 3.17SGLTGEEE421 pKa = 4.35SSSGQLGPTAEEE433 pKa = 5.2FTTDDD438 pKa = 3.17SGLTGEEE445 pKa = 4.35SSSGQLGPTAEEE457 pKa = 5.2FTTDDD462 pKa = 3.17SGLTGEEE469 pKa = 4.35SSSGQLGPTAEEE481 pKa = 5.2FTTDDD486 pKa = 3.17SGLTGEEE493 pKa = 4.35SSSGQLGPTAEEE505 pKa = 5.2FTTDDD510 pKa = 3.17SGLTGEEE517 pKa = 4.35SSSGQLGPTAEEE529 pKa = 5.2FTTDDD534 pKa = 3.17SGLTGEEE541 pKa = 4.35SSSGQLGPTAEEE553 pKa = 5.2FTTDDD558 pKa = 3.17SGLTGEEE565 pKa = 4.35SSSGQLGPTSMICKKK580 pKa = 9.4SSPSRR585 pKa = 11.84RR586 pKa = 11.84YYY588 pKa = 9.47VGAHHH593 pKa = 5.98DDD595 pKa = 3.86IKKK598 pKa = 10.46HH599 pKa = 6.15GAIRR603 pKa = 11.84YYY605 pKa = 7.26GICQTLSMHHH615 pKa = 7.01LPFKKK620 pKa = 10.46LRR622 pKa = 11.84LMFLKKK628 pKa = 10.51DD629 pKa = 3.06SYYY632 pKa = 10.73YYY634 pKa = 9.99DDD636 pKa = 3.76IYYY639 pKa = 10.3IKKK642 pKa = 10.4TVQINIVKKK651 pKa = 7.77YY652 pKa = 9.67YY653 pKa = 10.74YYY655 pKa = 10.3LC

Molecular weight: 67.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A672MC37|A0A672MC37_SINGR A kinase (PRKA) anchor protein 7 OS=Sinocyclocheilus grahami OX=75366 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84SAAAMDD8 pKa = 3.55VGVTVLLHH16 pKa = 5.55IQVFVIFSFGQLLIVGGGGGRR37 pKa = 11.84LWPGPGQRR45 pKa = 11.84LWPGPGQRR53 pKa = 11.84LWPGPGQRR61 pKa = 11.84LWAGPGQRR69 pKa = 11.84LWSRR73 pKa = 11.84PGKK76 pKa = 10.04RR77 pKa = 11.84LWPGKK82 pKa = 9.54RR83 pKa = 11.84LWSRR87 pKa = 11.84PGKK90 pKa = 10.04RR91 pKa = 11.84LWPGKK96 pKa = 9.62RR97 pKa = 11.84LWPGKK102 pKa = 9.62RR103 pKa = 11.84LWPGKK108 pKa = 9.62RR109 pKa = 11.84LWPGKK114 pKa = 9.62RR115 pKa = 11.84LWPGKK120 pKa = 9.62RR121 pKa = 11.84LWPGKK126 pKa = 9.62RR127 pKa = 11.84LWPGKK132 pKa = 9.62RR133 pKa = 11.84LWPGKK138 pKa = 9.54RR139 pKa = 11.84LWSRR143 pKa = 11.84PGKK146 pKa = 10.04RR147 pKa = 11.84LWPGKK152 pKa = 9.54RR153 pKa = 11.84LWSRR157 pKa = 11.84PGKK160 pKa = 10.04RR161 pKa = 11.84LWPGKK166 pKa = 9.54RR167 pKa = 11.84LWSRR171 pKa = 11.84PGKK174 pKa = 10.04RR175 pKa = 11.84LWPGKK180 pKa = 10.27RR181 pKa = 11.84PGKK184 pKa = 10.32RR185 pKa = 11.84PGKK188 pKa = 9.84RR189 pKa = 11.84LWPGKK194 pKa = 8.31WLWPGKK200 pKa = 7.46WARR203 pKa = 11.84KK204 pKa = 8.0WSMKK208 pKa = 10.39

Molecular weight: 24.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44175

57953

102128

62724889

18

8640

614.2

68.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.261 ± 0.007

2.319 ± 0.007

5.238 ± 0.005

6.816 ± 0.008

3.903 ± 0.006

6.007 ± 0.008

2.68 ± 0.003

4.885 ± 0.005

5.835 ± 0.008

9.708 ± 0.01

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.479 ± 0.003

4.041 ± 0.005

5.276 ± 0.008

4.661 ± 0.007

5.427 ± 0.005

8.322 ± 0.008

5.565 ± 0.006

6.426 ± 0.005

1.174 ± 0.003

2.952 ± 0.004
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski