Pacific flying fox faeces associated circular DNA virus-10

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTU9|A0A140CTU9_9VIRU ATP-dependent helicase Rep OS=Pacific flying fox faeces associated circular DNA virus-10 OX=1796005 PE=3 SV=1
MM1 pKa = 7.1SRR3 pKa = 11.84VRR5 pKa = 11.84VWVWTSFKK13 pKa = 11.07DD14 pKa = 4.68SLDD17 pKa = 4.01LNDD20 pKa = 4.73GGIEE24 pKa = 3.93YY25 pKa = 10.35SVYY28 pKa = 10.12QQEE31 pKa = 4.28QCPTTGRR38 pKa = 11.84LHH40 pKa = 5.45WQGVTRR46 pKa = 11.84FKK48 pKa = 10.88NARR51 pKa = 11.84QLGGVKK57 pKa = 9.8RR58 pKa = 11.84WLGDD62 pKa = 3.52PQAHH66 pKa = 6.68CEE68 pKa = 4.08PCKK71 pKa = 10.41KK72 pKa = 9.41EE73 pKa = 3.87HH74 pKa = 6.35EE75 pKa = 4.62AIKK78 pKa = 10.35YY79 pKa = 6.73CQKK82 pKa = 10.31EE83 pKa = 4.01EE84 pKa = 4.06TRR86 pKa = 11.84IGQATILGHH95 pKa = 5.04IQEE98 pKa = 4.52TLEE101 pKa = 4.3PGWWQKK107 pKa = 9.12LTINQLWEE115 pKa = 3.91QHH117 pKa = 5.98PEE119 pKa = 3.68WMLKK123 pKa = 10.08HH124 pKa = 5.46YY125 pKa = 10.87NGVQAYY131 pKa = 8.95RR132 pKa = 11.84RR133 pKa = 11.84SLNKK137 pKa = 10.36SQTEE141 pKa = 3.91RR142 pKa = 11.84QNIKK146 pKa = 10.14VFVFYY151 pKa = 8.01GTPGTGKK158 pKa = 10.16SYY160 pKa = 10.67SARR163 pKa = 11.84QFKK166 pKa = 10.36PYY168 pKa = 9.26FAKK171 pKa = 10.83ASGNWWDD178 pKa = 4.72GYY180 pKa = 10.7CGEE183 pKa = 4.43EE184 pKa = 3.93TVIFDD189 pKa = 4.48DD190 pKa = 5.64FYY192 pKa = 11.68GANEE196 pKa = 4.5KK197 pKa = 9.33YY198 pKa = 9.41TDD200 pKa = 3.26ILRR203 pKa = 11.84WCSEE207 pKa = 4.04LPISVPIKK215 pKa = 10.35GSMVPLEE222 pKa = 4.09ATTFIFTSNSHH233 pKa = 6.89PSLWWPGLLDD243 pKa = 3.21KK244 pKa = 11.17SAFQRR249 pKa = 11.84RR250 pKa = 11.84VTRR253 pKa = 11.84IYY255 pKa = 10.18QCFIEE260 pKa = 5.14KK261 pKa = 9.74FVKK264 pKa = 10.15QVWW267 pKa = 3.26

Molecular weight: 31.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTU9|A0A140CTU9_9VIRU ATP-dependent helicase Rep OS=Pacific flying fox faeces associated circular DNA virus-10 OX=1796005 PE=3 SV=1
MM1 pKa = 7.65AIVKK5 pKa = 9.56RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84VIRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84PVIRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 7.28YY22 pKa = 9.1GRR24 pKa = 11.84VARR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84LGRR32 pKa = 11.84RR33 pKa = 11.84SVRR36 pKa = 11.84VRR38 pKa = 11.84RR39 pKa = 11.84SRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84FAALPNWSKK52 pKa = 11.14RR53 pKa = 11.84DD54 pKa = 3.42YY55 pKa = 11.41SGISNLSNYY64 pKa = 9.43YY65 pKa = 10.15VVAQEE70 pKa = 4.3TPTVEE75 pKa = 3.92NSWYY79 pKa = 10.59HH80 pKa = 6.26LVFTQAQLYY89 pKa = 8.07PAFVQLWKK97 pKa = 10.35EE98 pKa = 3.69YY99 pKa = 8.92RR100 pKa = 11.84LAYY103 pKa = 10.1AVIAVRR109 pKa = 11.84RR110 pKa = 11.84RR111 pKa = 11.84NPQAVVYY118 pKa = 7.97STSSGSVTASRR129 pKa = 11.84VEE131 pKa = 4.45SNNIWWAPWAKK142 pKa = 10.15YY143 pKa = 9.72DD144 pKa = 5.2PPVQPPRR151 pKa = 11.84MVRR154 pKa = 11.84SAVLLTEE161 pKa = 4.18RR162 pKa = 11.84WTVRR166 pKa = 11.84KK167 pKa = 8.78LANKK171 pKa = 9.45CPYY174 pKa = 10.43YY175 pKa = 10.65DD176 pKa = 5.62FSWQPPFGTSTDD188 pKa = 3.11ISGVPDD194 pKa = 3.4IKK196 pKa = 10.8IPSRR200 pKa = 11.84VIAASLFDD208 pKa = 5.39ADD210 pKa = 4.04TTTPTSSEE218 pKa = 3.76QSLLKK223 pKa = 10.3TFGQFTARR231 pKa = 11.84WRR233 pKa = 11.84SVPFQSVDD241 pKa = 3.18TQEE244 pKa = 4.37QVVHH248 pKa = 6.61CGFFCTEE255 pKa = 4.02NKK257 pKa = 10.3SAIQPDD263 pKa = 4.73LIEE266 pKa = 3.96VCARR270 pKa = 11.84VVFQMRR276 pKa = 11.84GRR278 pKa = 11.84KK279 pKa = 8.84NLGTEE284 pKa = 4.23TDD286 pKa = 3.09SSGAFDD292 pKa = 3.34AAEE295 pKa = 3.92VVEE298 pKa = 5.4FSMDD302 pKa = 3.37QRR304 pKa = 11.84GTAPPKK310 pKa = 10.32LRR312 pKa = 11.84YY313 pKa = 9.62GPLKK317 pKa = 10.46ALPDD321 pKa = 3.74CSSFKK326 pKa = 10.46TSNPTDD332 pKa = 3.38LHH334 pKa = 5.86VQQITCSS341 pKa = 3.4

Molecular weight: 39.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

608

267

341

304.0

35.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.25 ± 1.321

2.138 ± 0.3

3.783 ± 0.256

4.934 ± 1.121

4.605 ± 0.163

5.428 ± 1.279

1.645 ± 0.606

4.441 ± 0.498

5.428 ± 1.047

5.921 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.151 ± 0.017

3.289 ± 0.05

5.757 ± 0.783

5.921 ± 0.741

9.046 ± 2.125

7.895 ± 1.179

6.579 ± 0.131

7.895 ± 1.412

3.783 ± 0.906

4.112 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski