Enterococcus phage phiFL3B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Phifelvirus; Enterococcus virus FL3

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2IZY9|D2IZY9_9CAUD Uncharacterized protein gp33 OS=Enterococcus phage phiFL3B OX=673838 GN=gp33 PE=4 SV=1
MM1 pKa = 7.35IPKK4 pKa = 9.8FRR6 pKa = 11.84ARR8 pKa = 11.84DD9 pKa = 3.49QRR11 pKa = 11.84GNWHH15 pKa = 6.14VGLLTFMFGQYY26 pKa = 10.82AIVNEE31 pKa = 4.01SDD33 pKa = 3.49EE34 pKa = 4.72NSICLIDD41 pKa = 4.92KK42 pKa = 7.56EE43 pKa = 5.03TIGQSIGLKK52 pKa = 10.2DD53 pKa = 3.67KK54 pKa = 11.31NGVDD58 pKa = 3.14IFEE61 pKa = 5.0GDD63 pKa = 3.34GVQYY67 pKa = 10.89RR68 pKa = 11.84DD69 pKa = 3.43GEE71 pKa = 4.21YY72 pKa = 10.38SYY74 pKa = 11.55LGIVKK79 pKa = 9.75RR80 pKa = 11.84DD81 pKa = 3.49CYY83 pKa = 11.08QFFIDD88 pKa = 6.53GIEE91 pKa = 4.16PDD93 pKa = 4.34DD94 pKa = 4.62NYY96 pKa = 11.81DD97 pKa = 4.71FIDD100 pKa = 3.64VSNTFDD106 pKa = 3.46GTSSLEE112 pKa = 3.64ILGNIHH118 pKa = 6.68EE119 pKa = 4.55NPEE122 pKa = 4.16LLEE125 pKa = 4.24GTEE128 pKa = 4.1

Molecular weight: 14.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2IZZ9|D2IZZ9_9CAUD Uncharacterized protein gp43 OS=Enterococcus phage phiFL3B OX=673838 GN=gp43 PE=4 SV=1
MM1 pKa = 8.19DD2 pKa = 4.69FLRR5 pKa = 11.84WIFVDD10 pKa = 3.79NDD12 pKa = 3.81GNFQWMSITALISAGGVWATFNRR35 pKa = 11.84SKK37 pKa = 10.58KK38 pKa = 9.01QYY40 pKa = 10.33RR41 pKa = 11.84LTFILKK47 pKa = 9.43QRR49 pKa = 11.84LKK51 pKa = 10.88SS52 pKa = 3.5

Molecular weight: 6.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

12142

32

1511

184.0

20.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.515 ± 0.479

0.585 ± 0.104

6.07 ± 0.289

8.046 ± 0.616

3.805 ± 0.176

6.259 ± 0.514

1.244 ± 0.143

7.083 ± 0.23

9.496 ± 0.336

8.714 ± 0.371

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.331 ± 0.124

5.93 ± 0.257

3.014 ± 0.171

3.805 ± 0.23

3.994 ± 0.256

6.671 ± 0.339

5.189 ± 0.275

6.185 ± 0.185

1.277 ± 0.146

3.789 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski