Marbled eel polyomavirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; unclassified Polyomaviridae

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A161IWK5|A0A161IWK5_9POLY ORF02R OS=Marbled eel polyomavirus OX=1662286 PE=4 SV=1
MM1 pKa = 7.79ASQPKK6 pKa = 9.76RR7 pKa = 11.84FKK9 pKa = 10.58QGSEE13 pKa = 4.12EE14 pKa = 4.17EE15 pKa = 4.03EE16 pKa = 4.01DD17 pKa = 3.99VYY19 pKa = 11.08GTVVFSDD26 pKa = 3.06CHH28 pKa = 6.8IVVNGNVSIVVNIDD42 pKa = 2.82AAGAEE47 pKa = 4.28EE48 pKa = 4.33QEE50 pKa = 4.62GDD52 pKa = 4.06TGDD55 pKa = 4.02DD56 pKa = 3.71TPTSDD61 pKa = 5.21GGEE64 pKa = 4.04QAQGEE69 pKa = 4.55SAEE72 pKa = 4.48AEE74 pKa = 4.04AEE76 pKa = 4.21EE77 pKa = 5.17PGDD80 pKa = 5.04SPICFQAFVPPPPPPPGPVALCCVDD105 pKa = 3.46AAQGEE110 pKa = 4.65EE111 pKa = 4.32EE112 pKa = 4.69GDD114 pKa = 3.74ANPTDD119 pKa = 4.16GADD122 pKa = 3.45EE123 pKa = 4.79DD124 pKa = 4.89GEE126 pKa = 4.38GGIDD130 pKa = 3.32VGRR133 pKa = 11.84RR134 pKa = 11.84LPDD137 pKa = 3.29GCEE140 pKa = 3.81TDD142 pKa = 3.39GGLSFEE148 pKa = 4.76AGLMVKK154 pKa = 10.18AHH156 pKa = 6.11IQRR159 pKa = 11.84SVDD162 pKa = 3.29QQDD165 pKa = 3.8SVRR168 pKa = 11.84QPSTSTQAAPAASEE182 pKa = 4.14TDD184 pKa = 3.99GPVHH188 pKa = 7.38PDD190 pKa = 2.72TGEE193 pKa = 3.95AVAPAAPAAPAAEE206 pKa = 4.7PEE208 pKa = 4.35PTPAPVPIPAPPPPAAAALTPTAAPVSPQPAAEE241 pKa = 4.51GSAPAPAAPAVV252 pKa = 3.49

Molecular weight: 25.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A168T4T6|A0A168T4T6_9POLY ORF01L OS=Marbled eel polyomavirus OX=1662286 PE=4 SV=1
MM1 pKa = 8.01DD2 pKa = 4.54GARR5 pKa = 11.84RR6 pKa = 11.84HH7 pKa = 5.87GGGVGPWMRR16 pKa = 11.84GAGGGPYY23 pKa = 8.93WDD25 pKa = 4.37SSAAAQKK32 pKa = 9.54WLSRR36 pKa = 11.84QAGKK40 pKa = 10.54RR41 pKa = 11.84GWKK44 pKa = 8.45NRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84GGGLNIKK55 pKa = 9.73QLASKK60 pKa = 9.65AWQGIRR66 pKa = 11.84NNLIPLISRR75 pKa = 11.84HH76 pKa = 5.41ARR78 pKa = 11.84SFLSQAAEE86 pKa = 3.73HH87 pKa = 6.5AVQNGPQYY95 pKa = 11.39YY96 pKa = 10.03SAFKK100 pKa = 10.41QGGVQGLRR108 pKa = 11.84DD109 pKa = 3.85SVLDD113 pKa = 4.18NLPGMAGSFIRR124 pKa = 11.84SVAKK128 pKa = 9.54TGRR131 pKa = 11.84GSGPHH136 pKa = 6.84CATIGQMLLSARR148 pKa = 11.84PHH150 pKa = 5.66IQSVTLPMPSHH161 pKa = 6.37LRR163 pKa = 11.84SEE165 pKa = 4.64GLNRR169 pKa = 11.84AYY171 pKa = 10.74AKK173 pKa = 9.05MARR176 pKa = 11.84GEE178 pKa = 4.24SLNPYY183 pKa = 9.51SQDD186 pKa = 3.16DD187 pKa = 3.81CAAPFLAVQDD197 pKa = 3.97CALRR201 pKa = 11.84EE202 pKa = 4.35AVKK205 pKa = 10.7HH206 pKa = 5.25MNPEE210 pKa = 3.76EE211 pKa = 4.26RR212 pKa = 11.84GGFLPLLIPLLGGAISSLVGSIPKK236 pKa = 9.85FVEE239 pKa = 3.51LAQANKK245 pKa = 9.9RR246 pKa = 11.84GKK248 pKa = 10.76GSDD251 pKa = 3.67LLGMFSPPPFTSSGHH266 pKa = 4.98SPRR269 pKa = 11.84GAGPRR274 pKa = 11.84ASTFLTAQFEE284 pKa = 4.48NGATVQMSRR293 pKa = 11.84RR294 pKa = 11.84KK295 pKa = 10.04SEE297 pKa = 3.7EE298 pKa = 3.75TPYY301 pKa = 10.84KK302 pKa = 10.4IIVKK306 pKa = 10.22KK307 pKa = 10.64EE308 pKa = 3.76GGGSRR313 pKa = 11.84GRR315 pKa = 11.84PRR317 pKa = 11.84QLTWYY322 pKa = 10.4ASADD326 pKa = 3.67LFF328 pKa = 4.86

Molecular weight: 35.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16

0

16

4658

104

820

291.1

32.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.128 ± 0.719

1.889 ± 0.345

5.303 ± 0.621

5.174 ± 0.577

3.392 ± 0.363

6.977 ± 0.842

2.748 ± 0.281

4.165 ± 0.443

3.242 ± 0.427

8.437 ± 0.674

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.533 ± 0.249

4.122 ± 0.416

9.124 ± 1.3

4.938 ± 0.308

6.977 ± 0.483

8.416 ± 0.518

5.539 ± 0.488

6.526 ± 0.373

0.859 ± 0.166

2.512 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski