Amino acid dipepetide frequency for Bacillus phage Claudi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.541AlaAla: 0.541 ± 0.259
0.947AlaCys: 0.947 ± 0.341
2.03AlaAsp: 2.03 ± 0.395
3.383AlaGlu: 3.383 ± 0.9
2.436AlaPhe: 2.436 ± 0.514
3.113AlaGly: 3.113 ± 0.835
0.0AlaHis: 0.0 ± 0.0
3.248AlaIle: 3.248 ± 0.736
3.519AlaLys: 3.519 ± 0.623
2.436AlaLeu: 2.436 ± 0.458
1.895AlaMet: 1.895 ± 0.452
2.301AlaAsn: 2.301 ± 0.417
1.353AlaPro: 1.353 ± 0.464
2.301AlaGln: 2.301 ± 0.551
1.489AlaArg: 1.489 ± 0.358
2.301AlaSer: 2.301 ± 0.519
2.165AlaThr: 2.165 ± 0.467
2.301AlaVal: 2.301 ± 0.658
0.677AlaTrp: 0.677 ± 0.332
2.03AlaTyr: 2.03 ± 0.412
0.0AlaXaa: 0.0 ± 0.0
Cys
0.406CysAla: 0.406 ± 0.269
0.135CysCys: 0.135 ± 0.164
1.624CysAsp: 1.624 ± 0.475
1.353CysGlu: 1.353 ± 0.369
0.541CysPhe: 0.541 ± 0.302
1.353CysGly: 1.353 ± 0.437
0.135CysHis: 0.135 ± 0.109
0.406CysIle: 0.406 ± 0.206
1.489CysLys: 1.489 ± 0.546
0.947CysLeu: 0.947 ± 0.297
0.406CysMet: 0.406 ± 0.229
0.271CysAsn: 0.271 ± 0.204
0.677CysPro: 0.677 ± 0.354
0.0CysGln: 0.0 ± 0.0
0.406CysArg: 0.406 ± 0.263
0.271CysSer: 0.271 ± 0.18
0.271CysThr: 0.271 ± 0.277
0.406CysVal: 0.406 ± 0.267
0.135CysTrp: 0.135 ± 0.111
0.677CysTyr: 0.677 ± 0.249
0.0CysXaa: 0.0 ± 0.0
Asp
2.436AspAla: 2.436 ± 0.453
1.489AspCys: 1.489 ± 0.516
3.789AspAsp: 3.789 ± 0.705
5.413AspGlu: 5.413 ± 0.73
3.925AspPhe: 3.925 ± 0.542
5.143AspGly: 5.143 ± 1.104
0.947AspHis: 0.947 ± 0.391
5.143AspIle: 5.143 ± 0.766
5.549AspLys: 5.549 ± 0.644
4.06AspLeu: 4.06 ± 0.72
2.707AspMet: 2.707 ± 0.582
3.654AspAsn: 3.654 ± 0.614
2.301AspPro: 2.301 ± 0.533
2.707AspGln: 2.707 ± 0.484
1.759AspArg: 1.759 ± 0.475
2.842AspSer: 2.842 ± 0.655
2.707AspThr: 2.707 ± 0.547
5.549AspVal: 5.549 ± 0.752
0.406AspTrp: 0.406 ± 0.193
2.842AspTyr: 2.842 ± 0.722
0.0AspXaa: 0.0 ± 0.0
Glu
3.113GluAla: 3.113 ± 0.74
0.541GluCys: 0.541 ± 0.225
3.925GluAsp: 3.925 ± 0.701
6.767GluGlu: 6.767 ± 1.505
4.331GluPhe: 4.331 ± 0.902
4.331GluGly: 4.331 ± 0.752
0.677GluHis: 0.677 ± 0.306
6.902GluIle: 6.902 ± 0.937
5.819GluLys: 5.819 ± 1.128
8.526GluLeu: 8.526 ± 1.371
2.707GluMet: 2.707 ± 0.718
5.549GluAsn: 5.549 ± 0.838
0.947GluPro: 0.947 ± 0.348
2.842GluGln: 2.842 ± 0.601
3.925GluArg: 3.925 ± 0.85
4.195GluSer: 4.195 ± 0.725
4.601GluThr: 4.601 ± 1.05
6.09GluVal: 6.09 ± 0.932
1.083GluTrp: 1.083 ± 0.269
4.195GluTyr: 4.195 ± 0.778
0.0GluXaa: 0.0 ± 0.0
Phe
1.759PheAla: 1.759 ± 0.447
0.541PheCys: 0.541 ± 0.257
3.519PheAsp: 3.519 ± 0.579
4.06PheGlu: 4.06 ± 0.826
1.489PhePhe: 1.489 ± 0.363
2.301PheGly: 2.301 ± 0.667
0.947PheHis: 0.947 ± 0.376
4.872PheIle: 4.872 ± 0.786
5.007PheLys: 5.007 ± 0.863
2.977PheLeu: 2.977 ± 0.527
2.03PheMet: 2.03 ± 0.549
3.113PheAsn: 3.113 ± 0.841
0.812PhePro: 0.812 ± 0.327
0.812PheGln: 0.812 ± 0.35
1.083PheArg: 1.083 ± 0.335
3.248PheSer: 3.248 ± 0.835
2.436PheThr: 2.436 ± 0.606
2.707PheVal: 2.707 ± 0.689
0.406PheTrp: 0.406 ± 0.193
2.03PheTyr: 2.03 ± 0.531
0.0PheXaa: 0.0 ± 0.0
Gly
2.977GlyAla: 2.977 ± 0.843
0.271GlyCys: 0.271 ± 0.232
2.707GlyAsp: 2.707 ± 0.669
4.466GlyGlu: 4.466 ± 0.634
3.519GlyPhe: 3.519 ± 0.491
4.06GlyGly: 4.06 ± 0.915
0.947GlyHis: 0.947 ± 0.357
4.872GlyIle: 4.872 ± 0.846
6.361GlyLys: 6.361 ± 1.164
4.872GlyLeu: 4.872 ± 0.804
2.165GlyMet: 2.165 ± 0.517
4.331GlyAsn: 4.331 ± 0.943
0.271GlyPro: 0.271 ± 0.152
2.03GlyGln: 2.03 ± 0.513
1.895GlyArg: 1.895 ± 0.464
4.737GlySer: 4.737 ± 0.76
5.819GlyThr: 5.819 ± 1.246
3.654GlyVal: 3.654 ± 0.727
0.406GlyTrp: 0.406 ± 0.234
2.977GlyTyr: 2.977 ± 0.543
0.0GlyXaa: 0.0 ± 0.0
His
0.677HisAla: 0.677 ± 0.259
0.135HisCys: 0.135 ± 0.132
1.083HisAsp: 1.083 ± 0.459
0.677HisGlu: 0.677 ± 0.361
0.947HisPhe: 0.947 ± 0.291
0.271HisGly: 0.271 ± 0.209
0.541HisHis: 0.541 ± 0.284
1.624HisIle: 1.624 ± 0.409
1.624HisLys: 1.624 ± 0.527
1.489HisLeu: 1.489 ± 0.483
0.271HisMet: 0.271 ± 0.155
0.541HisAsn: 0.541 ± 0.2
0.0HisPro: 0.0 ± 0.0
0.677HisGln: 0.677 ± 0.313
0.406HisArg: 0.406 ± 0.199
0.406HisSer: 0.406 ± 0.289
1.353HisThr: 1.353 ± 0.334
0.541HisVal: 0.541 ± 0.298
0.135HisTrp: 0.135 ± 0.133
1.218HisTyr: 1.218 ± 0.385
0.0HisXaa: 0.0 ± 0.0
Ile
2.707IleAla: 2.707 ± 0.496
0.541IleCys: 0.541 ± 0.277
6.496IleAsp: 6.496 ± 0.744
8.12IleGlu: 8.12 ± 1.282
2.301IlePhe: 2.301 ± 0.457
4.872IleGly: 4.872 ± 0.64
0.812IleHis: 0.812 ± 0.337
5.684IleIle: 5.684 ± 1.199
6.631IleLys: 6.631 ± 1.032
2.842IleLeu: 2.842 ± 0.677
2.707IleMet: 2.707 ± 0.552
5.549IleAsn: 5.549 ± 0.749
1.895IlePro: 1.895 ± 0.389
2.571IleGln: 2.571 ± 0.415
4.331IleArg: 4.331 ± 0.828
3.248IleSer: 3.248 ± 0.651
4.737IleThr: 4.737 ± 0.857
4.331IleVal: 4.331 ± 0.603
0.812IleTrp: 0.812 ± 0.281
2.842IleTyr: 2.842 ± 0.721
0.0IleXaa: 0.0 ± 0.0
Lys
3.789LysAla: 3.789 ± 0.727
1.083LysCys: 1.083 ± 0.456
6.631LysAsp: 6.631 ± 1.223
9.203LysGlu: 9.203 ± 1.392
3.654LysPhe: 3.654 ± 0.74
6.631LysGly: 6.631 ± 0.984
1.624LysHis: 1.624 ± 0.351
5.819LysIle: 5.819 ± 0.728
10.015LysLys: 10.015 ± 1.078
7.985LysLeu: 7.985 ± 1.121
3.248LysMet: 3.248 ± 0.749
5.684LysAsn: 5.684 ± 0.871
0.812LysPro: 0.812 ± 0.32
3.248LysGln: 3.248 ± 0.917
4.06LysArg: 4.06 ± 0.695
4.06LysSer: 4.06 ± 0.755
5.955LysThr: 5.955 ± 0.861
6.225LysVal: 6.225 ± 0.719
1.083LysTrp: 1.083 ± 0.331
3.925LysTyr: 3.925 ± 0.673
0.0LysXaa: 0.0 ± 0.0
Leu
2.571LeuAla: 2.571 ± 0.514
0.406LeuCys: 0.406 ± 0.252
5.007LeuAsp: 5.007 ± 0.895
5.819LeuGlu: 5.819 ± 1.046
2.436LeuPhe: 2.436 ± 0.656
4.331LeuGly: 4.331 ± 0.915
1.624LeuHis: 1.624 ± 0.458
5.413LeuIle: 5.413 ± 0.915
8.932LeuLys: 8.932 ± 1.526
5.007LeuLeu: 5.007 ± 0.644
1.895LeuMet: 1.895 ± 0.488
4.737LeuAsn: 4.737 ± 0.623
2.301LeuPro: 2.301 ± 0.491
3.789LeuGln: 3.789 ± 0.784
2.842LeuArg: 2.842 ± 0.554
4.06LeuSer: 4.06 ± 0.631
3.925LeuThr: 3.925 ± 0.698
3.925LeuVal: 3.925 ± 0.715
0.947LeuTrp: 0.947 ± 0.33
2.571LeuTyr: 2.571 ± 0.503
0.0LeuXaa: 0.0 ± 0.0
Met
0.812MetAla: 0.812 ± 0.283
0.541MetCys: 0.541 ± 0.301
2.436MetAsp: 2.436 ± 0.532
2.03MetGlu: 2.03 ± 0.622
2.301MetPhe: 2.301 ± 0.55
2.03MetGly: 2.03 ± 0.503
0.135MetHis: 0.135 ± 0.132
1.895MetIle: 1.895 ± 0.521
2.571MetLys: 2.571 ± 0.549
2.436MetLeu: 2.436 ± 0.61
1.218MetMet: 1.218 ± 0.487
3.654MetAsn: 3.654 ± 0.708
0.812MetPro: 0.812 ± 0.306
0.677MetGln: 0.677 ± 0.259
1.353MetArg: 1.353 ± 0.405
2.301MetSer: 2.301 ± 0.504
1.759MetThr: 1.759 ± 0.485
1.218MetVal: 1.218 ± 0.36
0.947MetTrp: 0.947 ± 0.331
1.624MetTyr: 1.624 ± 0.383
0.0MetXaa: 0.0 ± 0.0
Asn
2.165AsnAla: 2.165 ± 0.707
1.218AsnCys: 1.218 ± 0.459
4.872AsnAsp: 4.872 ± 0.669
5.684AsnGlu: 5.684 ± 0.683
3.519AsnPhe: 3.519 ± 0.816
3.925AsnGly: 3.925 ± 0.841
0.677AsnHis: 0.677 ± 0.33
4.466AsnIle: 4.466 ± 0.788
6.361AsnLys: 6.361 ± 1.015
4.601AsnLeu: 4.601 ± 0.805
1.353AsnMet: 1.353 ± 0.528
6.361AsnAsn: 6.361 ± 1.221
2.165AsnPro: 2.165 ± 0.603
2.977AsnGln: 2.977 ± 0.518
2.842AsnArg: 2.842 ± 0.762
4.06AsnSer: 4.06 ± 0.713
3.654AsnThr: 3.654 ± 0.877
5.413AsnVal: 5.413 ± 0.924
0.812AsnTrp: 0.812 ± 0.244
2.977AsnTyr: 2.977 ± 0.698
0.0AsnXaa: 0.0 ± 0.0
Pro
1.353ProAla: 1.353 ± 0.45
0.271ProCys: 0.271 ± 0.154
1.489ProAsp: 1.489 ± 0.396
1.624ProGlu: 1.624 ± 0.494
1.218ProPhe: 1.218 ± 0.452
0.541ProGly: 0.541 ± 0.226
0.135ProHis: 0.135 ± 0.117
1.353ProIle: 1.353 ± 0.427
1.759ProLys: 1.759 ± 0.538
1.218ProLeu: 1.218 ± 0.364
0.947ProMet: 0.947 ± 0.494
1.624ProAsn: 1.624 ± 0.498
0.406ProPro: 0.406 ± 0.177
0.406ProGln: 0.406 ± 0.195
0.677ProArg: 0.677 ± 0.315
1.624ProSer: 1.624 ± 0.452
2.165ProThr: 2.165 ± 0.535
1.895ProVal: 1.895 ± 0.503
0.271ProTrp: 0.271 ± 0.176
1.489ProTyr: 1.489 ± 0.584
0.0ProXaa: 0.0 ± 0.0
Gln
0.947GlnAla: 0.947 ± 0.342
0.271GlnCys: 0.271 ± 0.171
1.489GlnAsp: 1.489 ± 0.429
1.895GlnGlu: 1.895 ± 0.498
1.218GlnPhe: 1.218 ± 0.448
2.301GlnGly: 2.301 ± 0.599
0.406GlnHis: 0.406 ± 0.184
1.759GlnIle: 1.759 ± 0.529
3.383GlnLys: 3.383 ± 0.626
4.06GlnLeu: 4.06 ± 0.897
0.541GlnMet: 0.541 ± 0.244
3.248GlnAsn: 3.248 ± 0.514
0.947GlnPro: 0.947 ± 0.309
0.947GlnGln: 0.947 ± 0.343
1.489GlnArg: 1.489 ± 0.438
1.759GlnSer: 1.759 ± 0.391
2.436GlnThr: 2.436 ± 0.463
1.895GlnVal: 1.895 ± 0.419
0.541GlnTrp: 0.541 ± 0.282
2.977GlnTyr: 2.977 ± 0.572
0.0GlnXaa: 0.0 ± 0.0
Arg
2.842ArgAla: 2.842 ± 0.501
0.812ArgCys: 0.812 ± 0.302
1.895ArgAsp: 1.895 ± 0.557
2.977ArgGlu: 2.977 ± 0.749
2.842ArgPhe: 2.842 ± 0.456
2.436ArgGly: 2.436 ± 0.686
0.406ArgHis: 0.406 ± 0.194
2.301ArgIle: 2.301 ± 0.6
3.789ArgLys: 3.789 ± 0.731
2.571ArgLeu: 2.571 ± 0.615
1.353ArgMet: 1.353 ± 0.422
2.165ArgAsn: 2.165 ± 0.697
0.541ArgPro: 0.541 ± 0.255
2.03ArgGln: 2.03 ± 0.543
2.03ArgArg: 2.03 ± 0.516
2.165ArgSer: 2.165 ± 0.417
1.759ArgThr: 1.759 ± 0.531
2.03ArgVal: 2.03 ± 0.511
0.541ArgTrp: 0.541 ± 0.233
2.301ArgTyr: 2.301 ± 0.687
0.0ArgXaa: 0.0 ± 0.0
Ser
2.977SerAla: 2.977 ± 0.569
0.677SerCys: 0.677 ± 0.26
3.519SerAsp: 3.519 ± 0.701
4.06SerGlu: 4.06 ± 0.757
2.436SerPhe: 2.436 ± 0.647
3.113SerGly: 3.113 ± 0.715
1.353SerHis: 1.353 ± 0.348
3.925SerIle: 3.925 ± 0.822
4.331SerLys: 4.331 ± 0.657
3.925SerLeu: 3.925 ± 0.819
2.03SerMet: 2.03 ± 0.622
4.601SerAsn: 4.601 ± 0.862
0.541SerPro: 0.541 ± 0.25
2.165SerGln: 2.165 ± 0.605
2.165SerArg: 2.165 ± 0.529
2.436SerSer: 2.436 ± 0.761
2.03SerThr: 2.03 ± 0.415
3.383SerVal: 3.383 ± 0.638
0.135SerTrp: 0.135 ± 0.133
3.519SerTyr: 3.519 ± 0.62
0.0SerXaa: 0.0 ± 0.0
Thr
2.301ThrAla: 2.301 ± 0.575
0.541ThrCys: 0.541 ± 0.215
3.519ThrAsp: 3.519 ± 0.637
3.654ThrGlu: 3.654 ± 0.692
2.436ThrPhe: 2.436 ± 0.573
4.466ThrGly: 4.466 ± 1.195
1.083ThrHis: 1.083 ± 0.409
5.278ThrIle: 5.278 ± 0.636
4.872ThrLys: 4.872 ± 0.836
4.601ThrLeu: 4.601 ± 0.866
1.895ThrMet: 1.895 ± 0.502
3.789ThrAsn: 3.789 ± 0.733
1.895ThrPro: 1.895 ± 0.418
1.083ThrGln: 1.083 ± 0.337
2.436ThrArg: 2.436 ± 0.514
4.06ThrSer: 4.06 ± 0.768
4.737ThrThr: 4.737 ± 1.601
4.195ThrVal: 4.195 ± 0.729
0.541ThrTrp: 0.541 ± 0.249
1.353ThrTyr: 1.353 ± 0.433
0.0ThrXaa: 0.0 ± 0.0
Val
3.113ValAla: 3.113 ± 0.629
0.271ValCys: 0.271 ± 0.204
5.007ValAsp: 5.007 ± 0.847
5.684ValGlu: 5.684 ± 0.717
1.895ValPhe: 1.895 ± 0.492
4.872ValGly: 4.872 ± 0.64
1.218ValHis: 1.218 ± 0.397
4.737ValIle: 4.737 ± 0.848
6.631ValLys: 6.631 ± 0.672
4.195ValLeu: 4.195 ± 0.589
1.624ValMet: 1.624 ± 0.438
3.789ValAsn: 3.789 ± 0.762
2.571ValPro: 2.571 ± 0.686
1.489ValGln: 1.489 ± 0.396
2.301ValArg: 2.301 ± 0.62
3.519ValSer: 3.519 ± 0.561
3.248ValThr: 3.248 ± 0.7
4.331ValVal: 4.331 ± 0.855
0.812ValTrp: 0.812 ± 0.356
3.248ValTyr: 3.248 ± 0.69
0.0ValXaa: 0.0 ± 0.0
Trp
0.271TrpAla: 0.271 ± 0.181
0.271TrpCys: 0.271 ± 0.173
0.271TrpAsp: 0.271 ± 0.18
0.812TrpGlu: 0.812 ± 0.362
1.218TrpPhe: 1.218 ± 0.371
0.812TrpGly: 0.812 ± 0.298
0.541TrpHis: 0.541 ± 0.227
0.541TrpIle: 0.541 ± 0.261
1.218TrpLys: 1.218 ± 0.372
0.947TrpLeu: 0.947 ± 0.482
0.406TrpMet: 0.406 ± 0.209
1.083TrpAsn: 1.083 ± 0.365
0.0TrpPro: 0.0 ± 0.0
0.406TrpGln: 0.406 ± 0.233
0.947TrpArg: 0.947 ± 0.325
0.271TrpSer: 0.271 ± 0.206
0.271TrpThr: 0.271 ± 0.181
1.083TrpVal: 1.083 ± 0.402
0.271TrpTrp: 0.271 ± 0.243
0.541TrpTyr: 0.541 ± 0.249
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.707TyrAla: 2.707 ± 0.612
0.947TyrCys: 0.947 ± 0.32
4.06TyrAsp: 4.06 ± 0.654
3.383TyrGlu: 3.383 ± 0.574
1.624TyrPhe: 1.624 ± 0.301
2.436TyrGly: 2.436 ± 0.477
0.406TyrHis: 0.406 ± 0.22
3.789TyrIle: 3.789 ± 0.816
4.872TyrLys: 4.872 ± 0.664
2.842TyrLeu: 2.842 ± 0.657
1.218TyrMet: 1.218 ± 0.502
4.06TyrAsn: 4.06 ± 0.589
1.218TyrPro: 1.218 ± 0.293
1.353TyrGln: 1.353 ± 0.326
1.353TyrArg: 1.353 ± 0.309
1.759TyrSer: 1.759 ± 0.47
2.707TyrThr: 2.707 ± 0.525
3.383TyrVal: 3.383 ± 0.664
1.218TyrTrp: 1.218 ± 0.512
2.03TyrTyr: 2.03 ± 0.455
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (7390 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski